Restriction map for
pF1KB7133
(Circular) (Six-base) MAPSORT of: pF1KB7133.seq Check: 7528 from: 1 to: 4070
pF1KB7133 4070 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 882 882
Size: 4070
AccI GT'mk_AC
Cuts at: 659 1108 659
Size: 449 3621
Acc65I G'GTAC_C
Cuts at: 997 1086 997
Size: 89 3981
AclI AA'CG_TT
Cuts at: 3910 3910
Size: 4070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 1895 2327 3074 3342 221
Size: 1674 432 747 268 949
Fragments arranged by size:
1674 949 747 432 268
AflIII A'CryG_T
Cuts at: 1422 2526 3503 1422
Size: 1104 977 1989
Fragments arranged by size:
1989 1104 977
AgeI A'CCGG_T
Cuts at: 2458 2458
Size: 4070
AhdI GACnn_n'nnGTC
Cuts at: 176 176
Size: 4070
AleI CACnn'nnGTG
Cuts at: 374 374
Size: 4070
AlwNI CAG_nnn'CTG
Cuts at: 471 692 763 1375 2942 471
Size: 221 71 612 1567 1599
Fragments arranged by size:
1599 1567 612 221 71
ApaLI G'TGCA_C
Cuts at: 2840 2840
Size: 4070
ApoI r'AATT_y
Cuts at: 781 1074 3423 3679 781
Size: 293 2349 256 1172
Fragments arranged by size:
2349 1172 293 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4070
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
AvaI C'yCGr_G
Cuts at: 161 173 1090 161
Size: 12 917 3141
Fragments arranged by size:
3141 917 12
BamHI G'GATC_C
Cuts at: 1095 1095
Size: 4070
BanI G'GyrC_C
Cuts at: 358 894 997 1086 1500 1748 1783 358
Size: 536 103 89 414 248 35 2645
Fragments arranged by size:
2645 536 414 248 103 89 35
BanII G_rGCy'C
Cuts at: 303 424 1084 2114 303
Size: 121 660 1030 2259
Fragments arranged by size:
2259 1030 660 121
BbeI G_GCGC'C
Cuts at: 1504 1752 1504
Size: 248 3822
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4036
BciVI GTATCCnnnnn_n'
Cuts at: 1968 2729 4070 1968
Size: 761 1341 1968
Fragments arranged by size:
1968 1341 761
BclI T'GATC_A
Cuts at: 1590 1590
Size: 4070
BglII A'GATC_T
Cuts at: 773 858 1585 3622 773
Size: 85 727 2037 1221
Fragments arranged by size:
2037 1221 727 85
BlpI GC'TnA_GC
Cuts at: 1182 1182
Size: 4070
Bme1580I G_kGCm'C
Cuts at: 141 899 1695 1788 2844 141
Size: 758 796 93 1056 1367
Fragments arranged by size:
1367 1056 796 758 93
BmrI ACTGGGnnnn_n'
Cuts at: 1282 1363 1698 3842 1282
Size: 81 335 2144 1510
Fragments arranged by size:
2144 1510 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 837 2437 837
Size: 1600 2470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 994 901
Size: 93 3977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 625 1246 2125 2617 2915 550
Size: 75 621 879 492 298 1705
Fragments arranged by size:
1705 879 621 492 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 163 184 3774 163
Size: 21 3590 459
Fragments arranged by size:
3590 459 21
BsaAI yAC'GTr
Cuts at: 1423 1423
Size: 4070
BsaBI GATnn'nnATC
Cuts at: 6 1584 3621 6
Size: 1578 2037 455
Fragments arranged by size:
2037 1578 455
BsaHI Gr'CG_yC
Cuts at: 1501 1749 2451 3947 1501
Size: 248 702 1496 1624
Fragments arranged by size:
1624 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1259 1780 2458 2732 2879 3290 1259
Size: 521 678 274 147 411 2039
Fragments arranged by size:
2039 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 829
Size: 4070
BseYI C'CCAG_C
Cuts at: 898 1495 2253 2830 3326 898
Size: 597 758 577 496 1642
Fragments arranged by size:
1642 758 597 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 886 484
Size: 402 3668
BsiEI CG_ry'CG
Cuts at: 104 1612 1658 2866 104
Size: 1508 46 1208 1308
Fragments arranged by size:
1508 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 551 821 1084 1862 2052 2844 551
Size: 270 263 778 190 792 1777
Fragments arranged by size:
1777 792 778 270 263 190
BsmI GAATG_Cn'
Cuts at: 278 278
Size: 4070
Bsp1286I G_dGCh'C
Cuts at: 141 303 424 551 821 899 1084 1695 1788
Size: 162 121 127 270 78 185 611 93
Cuts at: 1788 1862 2052 2114 2844 141
Size: 74 190 62 730 1367
Fragments arranged by size:
1367 730 611 270 190 185 162 127
121 93 78 74 62
BspEI T'CCGG_A
Cuts at: 3290 3290
Size: 4070
BspHI T'CATG_A
Cuts at: 3246 3246
Size: 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 882 1120 1334 1636 2017 882
Size: 238 214 302 381 2935
Fragments arranged by size:
2935 381 302 238 214
BsrBI CCG'CTC
Cuts at: 50 2362 2416 3900 4065 50
Size: 2312 54 1484 165 55
Fragments arranged by size:
2312 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1982 1982
Size: 4070
BsrFI r'CCGG_y
Cuts at: 2068 2249 2458 2068
Size: 181 209 3680
Fragments arranged by size:
3680 209 181
BsrGI T'GTAC_A
Cuts at: 476 476
Size: 4070
BssSI C'ACGA_G
Cuts at: 113 2341 2699 113
Size: 2228 358 1484
Fragments arranged by size:
2228 1484 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1447 1447
Size: 4070
BstBI TT'CG_AA
Cuts at: 2431 2431
Size: 4070
BstXI CCAn_nnnn'nTGG
Cuts at: 861 861
Size: 4070
BstYI r'GATC_y
Cuts at: 1 432 560 773 858 1095 1562 1585 1920
Size: 431 128 213 85 237 467 23 335
Cuts at: 1920 2166 3178 3264 3276 3622 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 431 346 335 246 237
213 128 86 85 23 12
Bsu36I CC'TnA_GG
Cuts at: 191 191
Size: 4070
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2020 2201 2364 2020
Size: 181 163 3726
Fragments arranged by size:
3726 181 163
BtsI GCAGTG_nn'
Cuts at: 308 909 1313 308
Size: 601 404 3065
Fragments arranged by size:
3065 601 404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 910 945 910
Size: 35 4035
DraI TTT'AAA
Cuts at: 1069 1069
Size: 4070
DrdI GACnn_nn'nnGTC
Cuts at: 1776 2634 1776
Size: 858 3212
EaeI y'GGCC_r
Cuts at: 630 983 1655 1829 2220 2247 3592 630
Size: 353 672 174 391 27 1345 1108
Fragments arranged by size:
1345 1108 672 391 353 174 27
EagI C'GGCC_G
Cuts at: 1655 1655
Size: 4070
EarI CTCTTCn'nnn_
Cuts at: 118 209 240 764 1043 2093 2303 118
Size: 91 31 524 279 1050 210 1885
Fragments arranged by size:
1885 1050 524 279 210 91 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2278 2588 2734 3880 2278
Size: 310 146 1146 2468
Fragments arranged by size:
2468 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1082 1082
Size: 4070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 901 994 1895 2327 3074 3342 221
Size: 680 93 901 432 747 268 949
Fragments arranged by size:
949 901 747 680 432 268 93
EcoO109I rG'GnC_Cy
Cuts at: 219 582 1209 219
Size: 363 627 3080
Fragments arranged by size:
3080 627 363
EcoRI G'AATT_C
Cuts at: 781 1074 781
Size: 293 3777
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 4070
FspI TGC'GCA
Cuts at: 1851 1851
Size: 4070
HaeII r_GCGC'y
Cuts at: 1504 1752 2774 3753 1504
Size: 248 1022 979 1821
Fragments arranged by size:
1821 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 162 194 661 693 1912 1942 1944 1974 162
Size: 32 467 32 1219 30 2 30 2258
Fragments arranged by size:
2258 1219 467 32 32 30 30 2
HincII GTy'rAC
Cuts at: 621 1109 621
Size: 488 3582
HindIII A'AGCT_T
Cuts at: 712 1034 712
Size: 322 3748
KasI G'GCGC_C
Cuts at: 1500 1748 1500
Size: 248 3822
KpnI G_GTAC'C
Cuts at: 1001 1090 1001
Size: 89 3981
MluI A'CGCG_T
Cuts at: 3503 3503
Size: 4070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2741 2925 2741
Size: 184 3886
MscI TGG'CCA
Cuts at: 985 1831 985
Size: 846 3224
MslI CAynn'nnrTG
Cuts at: 374 2059 2186 374
Size: 1685 127 2258
Fragments arranged by size:
2258 1685 127
MspA1I CmG'CkG
Cuts at: 316 1181 1495 1855 2868 3113 316
Size: 865 314 360 1013 245 1273
Fragments arranged by size:
1273 1013 865 360 314 245
NaeI GCC'GGC
Cuts at: 2251 2251
Size: 4070
NarI GG'CG_CC
Cuts at: 1501 1749 1501
Size: 248 3822
NgoMIV G'CCGG_C
Cuts at: 2249 2249
Size: 4070
NspI r_CATG'y
Cuts at: 1123 1623 2530 1123
Size: 500 907 2663
Fragments arranged by size:
2663 907 500
PciI A'CATG_T
Cuts at: 2526 2526
Size: 4070
PfoI T'CCnGG_A
Cuts at: 971 971
Size: 4070
PmeI GTTT'AAAC
Cuts at: 1069 1069
Size: 4070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 4038
PstI C_TGCA'G
Cuts at: 1117 1802 1117
Size: 685 3385
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4070
PvuII CAG'CTG
Cuts at: 1495 1855 1495
Size: 360 3710
RsrII CG'GwC_CG
Cuts at: 2265 2265
Size: 4070
SacI G_AGCT'C
Cuts at: 1084 1084
Size: 4070
SalI G'TCGA_C
Cuts at: 1107 1107
Size: 4070
SapI GCTCTTCn'nnn_
Cuts at: 2093 2303 2093
Size: 210 3860
SbfI CC_TGCA'GG
Cuts at: 1117 1117
Size: 4070
ScaI AGT'ACT
Cuts at: 3587 3587
Size: 4070
SfcI C'TryA_G
Cuts at: 33 1113 1798 2791 2982 33
Size: 1080 685 993 191 1121
Fragments arranged by size:
1121 1080 993 685 191
SfoI GGC'GCC
Cuts at: 1502 1750 1502
Size: 248 3822
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
SmaI CCC'GGG
Cuts at: 175 1092 175
Size: 917 3153
SmlI C'TyrA_G
Cuts at: 565 604 1225 2140 2632 2894 565
Size: 39 621 915 492 262 1741
Fragments arranged by size:
1741 915 621 492 262 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4070
SphI G_CATG'C
Cuts at: 1123 1623 1123
Size: 500 3570
StuI AGG'CCT
Cuts at: 403 403
Size: 4070
StyI C'CwwG_G
Cuts at: 372 986 1204 1558 372
Size: 614 218 354 2884
Fragments arranged by size:
2884 614 354 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 721 1653 1786 2245 2454 721
Size: 932 133 459 209 2337
Fragments arranged by size:
2337 932 459 209 133
TatI w'GTAC_w
Cuts at: 476 884 3585 476
Size: 408 2701 961
Fragments arranged by size:
2701 961 408
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4038
Tth111I GACn'n_nGTC
Cuts at: 1867 1867
Size: 4070
XbaI T'CTAG_A
Cuts at: 1101 1101
Size: 4070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1565 1565
Size: 4070
XmaI C'CCGG_G
Cuts at: 173 1090 173
Size: 917 3153
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BssHII BstEII BstZ17I BtgI ClaI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI