Restriction map for
pF1KB7132
(Circular) (Six-base) MAPSORT of: pF1KB7132.seq Check: 6551 from: 1 to: 4067
pF1KB7132 4067 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 582 664 582
Size: 82 3985
AccI GT'mk_AC
Cuts at: 1105 1105
Size: 4067
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 552 1892 2324 3071 3339 139
Size: 413 1340 432 747 268 867
Fragments arranged by size:
1340 867 747 432 413 268
AfeI AGC'GCT
Cuts at: 133 133
Size: 4067
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 236 236
Size: 4067
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 412 444 412
Size: 32 4035
AlwNI CAG_nnn'CTG
Cuts at: 1372 2939 1372
Size: 1567 2500
ApaI G_GGCC'C
Cuts at: 948 948
Size: 4067
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 1071 3420 3676 1071
Size: 2349 256 1462
Fragments arranged by size:
2349 1462 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 171 307 897 1083 1497 1745 1780 171
Size: 136 590 186 414 248 35 2458
Fragments arranged by size:
2458 590 414 248 186 136 35
BanII G_rGCy'C
Cuts at: 948 1081 2111 948
Size: 133 1030 2904
Fragments arranged by size:
2904 1030 133
BbeI G_GCGC'C
Cuts at: 901 1501 1749 901
Size: 600 248 3219
Fragments arranged by size:
3219 600 248
BbvCI CC'TCA_GC
Cuts at: 143 206 143
Size: 63 4004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4033
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 242 693 1587 242
Size: 451 894 2722
Fragments arranged by size:
2722 894 451
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 948 1692 1785 2841 948
Size: 744 93 1056 2174
Fragments arranged by size:
2174 1056 744 93
BmrI ACTGGGnnnn_n'
Cuts at: 1279 1360 1695 3839 1279
Size: 81 335 2144 1507
Fragments arranged by size:
2144 1507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 327 2434 327
Size: 2107 1960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 904 997 439
Size: 465 93 3509
Fragments arranged by size:
3509 465 93
Bpu10I CC'TnA_GC
Cuts at: 143 206 841 143
Size: 63 635 3369
Fragments arranged by size:
3369 635 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 544 1243 2122 2614 2912 277
Size: 267 699 879 492 298 1432
Fragments arranged by size:
1432 879 699 492 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 3771 3771
Size: 4067
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 1581 3618 6
Size: 1575 2037 455
Fragments arranged by size:
2037 1575 455
BsaHI Gr'CG_yC
Cuts at: 898 1498 1746 2448 3944 898
Size: 600 248 702 1496 1021
Fragments arranged by size:
1496 1021 702 600 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2729 2876 3287 1256
Size: 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 412 442 412
Size: 30 4037
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 219 222 223 274 303 397 784 1063 219
Size: 3 1 51 29 94 387 279 3223
Fragments arranged by size:
3223 387 279 94 51 29 3 1
BseYI C'CCAG_C
Cuts at: 408 684 1492 2250 2827 3323 408
Size: 276 808 758 577 496 1152
Fragments arranged by size:
1152 808 758 577 496 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 889 211
Size: 678 3389
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 824 1081 1859 2049 2841 824
Size: 257 778 190 792 2050
Fragments arranged by size:
2050 792 778 257 190
Bsp1286I G_dGCh'C
Cuts at: 824 948 1081 1692 1785 1859 2049 2111 2841
Size: 124 133 611 93 74 190 62 730
Cuts at: 2841 824
Size: 2050
Fragments arranged by size:
2050 730 611 190 133 124 93 74
62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 3243 3243
Size: 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 582 664 1117 1331 1633 2014 582
Size: 82 453 214 302 381 2635
Fragments arranged by size:
2635 453 381 302 214 82
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 381 1979 381
Size: 1598 2469
BsrFI r'CCGG_y
Cuts at: 501 631 712 2065 2246 2455 501
Size: 130 81 1353 181 209 2113
Fragments arranged by size:
2113 1353 209 181 130 81
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstEII G'GTnAC_C
Cuts at: 278 278
Size: 4067
BstYI r'GATC_y
Cuts at: 1 1092 1559 1582 1917 2163 3175 3261 3273
Size: 1091 467 23 335 246 1012 86 12
Cuts at: 3273 3619 1
Size: 346 449
Fragments arranged by size:
1091 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 302 302
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 912 1310 912
Size: 398 3669
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DraIII CAC_nnn'GTG
Cuts at: 344 344
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 633 1652 1826 2217 2244 3589 633
Size: 1019 174 391 27 1345 1111
Fragments arranged by size:
1345 1111 1019 391 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 118 321 374 1028 2090 2300 118
Size: 203 53 654 1062 210 1885
Fragments arranged by size:
1885 1062 654 210 203 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 439 552 904 997 1892 2324 3071 3339
Size: 300 113 352 93 895 432 747 268
Cuts at: 3339 139
Size: 867
Fragments arranged by size:
895 867 747 432 352 300 268 113
93
EcoNI CCTnn'n_nnAGG
Cuts at: 259 259
Size: 4067
EcoO109I rG'GnC_Cy
Cuts at: 944 945 1206 944
Size: 1 261 3805
Fragments arranged by size:
3805 261 1
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
EcoRV GAT'ATC
Cuts at: 564 564
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 570 2086 570
Size: 1516 2551
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 135 901 1501 1749 2771 3750 135
Size: 766 600 248 1022 979 452
Fragments arranged by size:
1022 979 766 600 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 1909 1939 1941 1971 262
Size: 32 1615 30 2 30 2358
Fragments arranged by size:
2358 1615 32 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
KasI G'GCGC_C
Cuts at: 897 1497 1745 897
Size: 600 248 3219
Fragments arranged by size:
3219 600 248
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2922 2738
Size: 184 3883
MscI TGG'CCA
Cuts at: 1828 1828
Size: 4067
MslI CAynn'nnrTG
Cuts at: 592 610 2056 2183 592
Size: 18 1446 127 2476
Fragments arranged by size:
2476 1446 127 18
MspA1I CmG'CkG
Cuts at: 205 1178 1492 1852 2865 3110 205
Size: 973 314 360 1013 245 1162
Fragments arranged by size:
1162 1013 973 360 314 245
NaeI GCC'GGC
Cuts at: 633 2248 633
Size: 1615 2452
NarI GG'CG_CC
Cuts at: 898 1498 1746 898
Size: 600 248 3219
Fragments arranged by size:
3219 600 248
NgoMIV G'CCGG_C
Cuts at: 631 2246 631
Size: 1615 2452
NspI r_CATG'y
Cuts at: 591 985 1120 1620 2527 591
Size: 394 135 500 907 2131
Fragments arranged by size:
2131 907 500 394 135
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PfoI T'CCnGG_A
Cuts at: 431 974 431
Size: 543 3524
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 412 444 3206 3238 412
Size: 32 2762 32 1241
Fragments arranged by size:
2762 1241 32 32
PspOMI G'GGCC_C
Cuts at: 944 944
Size: 4067
PstI C_TGCA'G
Cuts at: 1114 1799 1114
Size: 685 3382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 205 1492 1852 205
Size: 1287 360 2420
Fragments arranged by size:
2420 1287 360
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 1110 1795 2788 2979 33
Size: 1077 685 993 191 1121
Fragments arranged by size:
1121 1077 993 685 191
SfoI GGC'GCC
Cuts at: 899 1499 1747 899
Size: 600 248 3219
Fragments arranged by size:
3219 600 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 256 523 1222 2137 2629 2891 256
Size: 267 699 915 492 262 1432
Fragments arranged by size:
1432 915 699 492 267 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 591 1120 1620 591
Size: 529 500 3038
Fragments arranged by size:
3038 529 500
StuI AGG'CCT
Cuts at: 152 152
Size: 4067
StyI C'CwwG_G
Cuts at: 162 940 1201 1555 162
Size: 778 261 354 2674
Fragments arranged by size:
2674 778 354 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 676 855 871 1650 1783 2242 2451 676
Size: 179 16 779 133 459 209 2292
Fragments arranged by size:
2292 779 459 209 179 133 16
TatI w'GTAC_w
Cuts at: 3582 3582
Size: 4067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 406 438 3584 3616 406
Size: 32 3146 32 857
Fragments arranged by size:
3146 857 32 32
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1562 1562
Size: 4067
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
XmnI GAAnn'nnTTC
Cuts at: 152 152
Size: 4067
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BaeI BbsI
BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I BtgI ClaI CspCI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI