Restriction map for
pF1KB7084
(Circular) (Six-base) MAPSORT of: pF1KB7084.seq Check: 9949 from: 1 to: 3494
pF1KB7084 3494 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 532 532
Size: 3494
Acc65I G'GTAC_C
Cuts at: 510 510
Size: 3494
AclI AA'CG_TT
Cuts at: 3334 3334
Size: 3494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1751 2498 2766 1319
Size: 432 747 268 2047
Fragments arranged by size:
2047 747 432 268
AfeI AGC'GCT
Cuts at: 196 196
Size: 3494
AflIII A'CryG_T
Cuts at: 846 1950 2927 846
Size: 1104 977 1413
Fragments arranged by size:
1413 1104 977
AgeI A'CCGG_T
Cuts at: 1882 1882
Size: 3494
AleI CACnn'nnGTG
Cuts at: 331 331
Size: 3494
AlwNI CAG_nnn'CTG
Cuts at: 799 2366 799
Size: 1567 1927
ApaI G_GGCC'C
Cuts at: 456 456
Size: 3494
ApaLI G'TGCA_C
Cuts at: 2264 2264
Size: 3494
ApoI r'AATT_y
Cuts at: 498 2847 3103 498
Size: 2349 256 889
Fragments arranged by size:
2349 889 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3494
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
AvaI C'yCGr_G
Cuts at: 466 514 466
Size: 48 3446
AvrII C'CTAG_G
Cuts at: 405 405
Size: 3494
BamHI G'GATC_C
Cuts at: 519 519
Size: 3494
BanI G'GyrC_C
Cuts at: 311 510 924 1172 1207 311
Size: 199 414 248 35 2598
Fragments arranged by size:
2598 414 248 199 35
BanII G_rGCy'C
Cuts at: 266 456 508 1538 266
Size: 190 52 1030 2222
Fragments arranged by size:
2222 1030 190 52
BbeI G_GCGC'C
Cuts at: 315 928 1176 315
Size: 613 248 2633
Fragments arranged by size:
2633 613 248
BciVI GTATCCnnnnn_n'
Cuts at: 1392 2153 3494 1392
Size: 761 1341 1392
Fragments arranged by size:
1392 1341 761
BclI T'GATC_A
Cuts at: 1014 1014
Size: 3494
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3494
BglII A'GATC_T
Cuts at: 1009 3046 1009
Size: 2037 1457
BlpI GC'TnA_GC
Cuts at: 265 606 265
Size: 341 3153
Bme1580I G_kGCm'C
Cuts at: 244 456 1119 1212 2268 244
Size: 212 663 93 1056 1470
Fragments arranged by size:
1470 1056 663 212 93
BmrI ACTGGGnnnn_n'
Cuts at: 706 787 1122 3266 706
Size: 81 335 2144 934
Fragments arranged by size:
2144 934 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 3494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 670 1549 2041 2339 121
Size: 549 879 492 298 1276
Fragments arranged by size:
1276 879 549 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3198 3198
Size: 3494
BsaAI yAC'GTr
Cuts at: 847 847
Size: 3494
BsaBI GATnn'nnATC
Cuts at: 6 1008 3045 6
Size: 1002 2037 455
Fragments arranged by size:
2037 1002 455
BsaHI Gr'CG_yC
Cuts at: 312 925 1173 1875 3371 312
Size: 613 248 702 1496 435
Fragments arranged by size:
1496 702 613 435 248
BsaWI w'CCGG_w
Cuts at: 683 1204 1882 2156 2303 2714 683
Size: 521 678 274 147 411 1463
Fragments arranged by size:
1463 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 919 1677 2254 2750 919
Size: 758 577 496 1663
Fragments arranged by size:
1663 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 252 1036 1082 2290 104
Size: 148 784 46 1208 1308
Fragments arranged by size:
1308 1208 784 148 46
BsiHKAI G_wGCw'C
Cuts at: 137 266 307 508 1286 1476 2268 137
Size: 129 41 201 778 190 792 1363
Fragments arranged by size:
1363 792 778 201 190 129 41
Bsp1286I G_dGCh'C
Cuts at: 137 244 266 307 456 508 1119 1212 1286
Size: 107 22 41 149 52 611 93 74
Cuts at: 1286 1476 1538 2268 137
Size: 190 62 730 1363
Fragments arranged by size:
1363 730 611 190 149 107 93 74
62 52 41 22
BspEI T'CCGG_A
Cuts at: 2714 2714
Size: 3494
BspHI T'CATG_A
Cuts at: 2670 2670
Size: 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 758 1060 1441 544
Size: 214 302 381 2597
Fragments arranged by size:
2597 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1786 1840 3324 3489 50
Size: 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1406 1406
Size: 3494
BsrFI r'CCGG_y
Cuts at: 251 1492 1673 1882 251
Size: 1241 181 209 1863
Fragments arranged by size:
1863 1241 209 181
BssSI C'ACGA_G
Cuts at: 481 1765 2123 481
Size: 1284 358 1852
Fragments arranged by size:
1852 1284 358
BstAPI GCAn_nnn'nTGC
Cuts at: 871 871
Size: 3494
BstBI TT'CG_AA
Cuts at: 1855 1855
Size: 3494
BstEII G'GTnAC_C
Cuts at: 333 333
Size: 3494
BstYI r'GATC_y
Cuts at: 1 378 519 986 1009 1344 1590 2602 2688
Size: 377 141 467 23 335 246 1012 86
Cuts at: 2688 2700 3046 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 377 346 335 246 141
86 23 12
BtgI C'CryG_G
Cuts at: 287 287
Size: 3494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1444 1625 1788 1444
Size: 181 163 3150
Fragments arranged by size:
3150 181 163
BtsI GCAGTG_nn'
Cuts at: 737 737
Size: 3494
DraI TTT'AAA
Cuts at: 493 493
Size: 3494
DrdI GACnn_nn'nnGTC
Cuts at: 483 1200 2058 483
Size: 717 858 1919
Fragments arranged by size:
1919 858 717
EaeI y'GGCC_r
Cuts at: 249 1079 1253 1644 1671 3016 249
Size: 830 174 391 27 1345 727
Fragments arranged by size:
1345 830 727 391 174 27
EagI C'GGCC_G
Cuts at: 249 1079 249
Size: 830 2664
EarI CTCTTCn'nnn_
Cuts at: 232 1517 1727 232
Size: 1285 210 1999
Fragments arranged by size:
1999 1285 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1702 2012 2158 3304 1702
Size: 310 146 1146 1892
Fragments arranged by size:
1892 1146 310 146
EcoICRI GAG'CTC
Cuts at: 264 506 264
Size: 242 3252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1319 1751 2498 2766 172
Size: 1147 432 747 268 900
Fragments arranged by size:
1147 900 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 476 633 476
Size: 157 3337
EcoRI G'AATT_C
Cuts at: 498 498
Size: 3494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1513 1513
Size: 3494
FseI GG_CCGG'CC
Cuts at: 255 255
Size: 3494
FspI TGC'GCA
Cuts at: 1275 1275
Size: 3494
HaeII r_GCGC'y
Cuts at: 198 315 928 1176 2198 3177 198
Size: 117 613 248 1022 979 515
Fragments arranged by size:
1022 979 613 515 248 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1336 1366 1368 1398 1336
Size: 30 2 30 3432
Fragments arranged by size:
3432 30 30 2
HincII GTy'rAC
Cuts at: 533 533
Size: 3494
KasI G'GCGC_C
Cuts at: 311 924 1172 311
Size: 613 248 2633
Fragments arranged by size:
2633 613 248
KpnI G_GTAC'C
Cuts at: 514 514
Size: 3494
MluI A'CGCG_T
Cuts at: 2927 2927
Size: 3494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2349 2165
Size: 184 3310
MscI TGG'CCA
Cuts at: 1255 1255
Size: 3494
MslI CAynn'nnrTG
Cuts at: 331 1483 1610 331
Size: 1152 127 2215
Fragments arranged by size:
2215 1152 127
MspA1I CmG'CkG
Cuts at: 164 180 365 605 919 1279 2292 2537 164
Size: 16 185 240 314 360 1013 245 1121
Fragments arranged by size:
1121 1013 360 314 245 240 185 16
NaeI GCC'GGC
Cuts at: 253 1675 253
Size: 1422 2072
NarI GG'CG_CC
Cuts at: 312 925 1173 312
Size: 613 248 2633
Fragments arranged by size:
2633 613 248
NgoMIV G'CCGG_C
Cuts at: 251 1673 251
Size: 1422 2072
NspI r_CATG'y
Cuts at: 547 1047 1954 547
Size: 500 907 2087
Fragments arranged by size:
2087 907 500
PciI A'CATG_T
Cuts at: 1950 1950
Size: 3494
PmeI GTTT'AAAC
Cuts at: 493 493
Size: 3494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2633 2665 2633
Size: 32 3462
PpuMI rG'GwC_Cy
Cuts at: 476 476
Size: 3494
PspOMI G'GGCC_C
Cuts at: 452 452
Size: 3494
PstI C_TGCA'G
Cuts at: 179 224 541 1226 179
Size: 45 317 685 2447
Fragments arranged by size:
2447 685 317 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3494
PvuII CAG'CTG
Cuts at: 180 919 1279 180
Size: 739 360 2395
Fragments arranged by size:
2395 739 360
RsrII CG'GwC_CG
Cuts at: 1689 1689
Size: 3494
SacI G_AGCT'C
Cuts at: 266 508 266
Size: 242 3252
SalI G'TCGA_C
Cuts at: 531 531
Size: 3494
SanDI GG'GwC_CC
Cuts at: 476 476
Size: 3494
SapI GCTCTTCn'nnn_
Cuts at: 232 1517 1727 232
Size: 1285 210 1999
Fragments arranged by size:
1999 1285 210
SbfI CC_TGCA'GG
Cuts at: 541 541
Size: 3494
ScaI AGT'ACT
Cuts at: 3011 3011
Size: 3494
SfcI C'TryA_G
Cuts at: 33 175 220 537 1222 2215 2406 33
Size: 142 45 317 685 993 191 1121
Fragments arranged by size:
1121 993 685 317 191 142 45
SfoI GGC'GCC
Cuts at: 313 926 1174 313
Size: 613 248 2633
Fragments arranged by size:
2633 613 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
SmaI CCC'GGG
Cuts at: 516 516
Size: 3494
SmlI C'TyrA_G
Cuts at: 136 649 1564 2056 2318 136
Size: 513 915 492 262 1312
Fragments arranged by size:
1312 915 513 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3494
SphI G_CATG'C
Cuts at: 547 1047 547
Size: 500 2994
StuI AGG'CCT
Cuts at: 410 410
Size: 3494
StyI C'CwwG_G
Cuts at: 236 257 405 628 982 236
Size: 21 148 223 354 2748
Fragments arranged by size:
2748 354 223 148 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 352 1077 1210 1669 1878 352
Size: 725 133 459 209 1968
Fragments arranged by size:
1968 725 459 209 133
TatI w'GTAC_w
Cuts at: 3009 3009
Size: 3494
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3462
Tth111I GACn'n_nGTC
Cuts at: 1291 1291
Size: 3494
XbaI T'CTAG_A
Cuts at: 525 525
Size: 3494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 989 989
Size: 3494
XmaI C'CCGG_G
Cuts at: 514 514
Size: 3494
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI