Restriction map for
pF1KB7007
(Circular) (Six-base) MAPSORT of: pF1KB7007.seq Check: 4622 from: 1 to: 7385
pF1KB7007 7385 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 266 1030 2114 2448 2763 2778 3398 266
Size: 764 1084 334 315 15 620 4253
Fragments arranged by size:
4253 1084 764 620 334 315 15
AccI GT'mk_AC
Cuts at: 1136 2966 3734 3968 4423 1136
Size: 1830 768 234 455 4098
Fragments arranged by size:
4098 1830 768 455 234
Acc65I G'GTAC_C
Cuts at: 1058 4401 1058
Size: 3343 4042
AclI AA'CG_TT
Cuts at: 7225 7225
Size: 7385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 946 1744 2615 3463 3472 3656 5210 5642
Size: 533 798 871 848 9 184 1554 432
Cuts at: 5642 6389 6657 413
Size: 747 268 1141
Fragments arranged by size:
1554 1141 871 848 798 747 533 432
268 184 9
AfeI AGC'GCT
Cuts at: 2732 2732
Size: 7385
AflIII A'CryG_T
Cuts at: 1280 1375 2079 2642 3304 4737 5841 6818 1280
Size: 95 704 563 662 1433 1104 977 1847
Fragments arranged by size:
1847 1433 1104 977 704 662 563 95
AgeI A'CCGG_T
Cuts at: 5773 5773
Size: 7385
AhdI GACnn_n'nnGTC
Cuts at: 3975 4062 3975
Size: 87 7298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 265 462 2362 4141 265
Size: 197 1900 1779 3509
Fragments arranged by size:
3509 1900 1779 197
AlwNI CAG_nnn'CTG
Cuts at: 423 507 597 675 1246 1774 2182 3589 3852
Size: 84 90 78 571 528 408 1407 263
Cuts at: 3852 4690 6257 423
Size: 838 1567 1551
Fragments arranged by size:
1567 1551 1407 838 571 528 408 263
90 84 78
ApaI G_GGCC'C
Cuts at: 778 1824 3381 3942 778
Size: 1046 1557 561 4221
Fragments arranged by size:
4221 1557 1046 561
ApaLI G'TGCA_C
Cuts at: 413 6155 413
Size: 5742 1643
ApoI r'AATT_y
Cuts at: 473 1618 2284 3091 3466 3567 3751 4389 6738
Size: 1145 666 807 375 101 184 638 2349
Cuts at: 6738 6994 473
Size: 256 864
Fragments arranged by size:
2349 1145 864 807 666 638 375 256
184 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 7385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7385
AvaI C'yCGr_G
Cuts at: 689 1497 2694 3380 3935 4405 689
Size: 808 1197 686 555 470 3669
Fragments arranged by size:
3669 1197 808 686 555 470
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1050 1083 1646 1679 1050
Size: 33 563 33 6756
Fragments arranged by size:
6756 563 33 33
BamHI G'GATC_C
Cuts at: 1440 4410 1440
Size: 2970 4415
BanI G'GyrC_C
Cuts at: 600 756 823 1058 1569 2945 4401 4815 5063
Size: 156 67 235 511 1376 1456 414 248
Cuts at: 5063 5098 600
Size: 35 2887
Fragments arranged by size:
2887 1456 1376 511 414 248 235 156
67 35
BanII G_rGCy'C
Cuts at: 629 778 1474 1814 1824 1858 2001 2029 3381
Size: 149 696 340 10 34 143 28 1352
Cuts at: 3381 3692 3905 3942 4088 4399 5429 629
Size: 311 213 37 146 311 1030 2585
Fragments arranged by size:
2585 1352 1030 696 340 311 311 213
149 146 143 37 34 28 10
BbeI G_GCGC'C
Cuts at: 827 4819 5067 827
Size: 3992 248 3145
Fragments arranged by size:
3992 3145 248
BbsI GAAGACnn'nnnn_
Cuts at: 142 1187 142
Size: 1045 6340
BbvCI CC'TCA_GC
Cuts at: 500 3853 4142 500
Size: 3353 289 3743
Fragments arranged by size:
3743 3353 289
BciVI GTATCCnnnnn_n'
Cuts at: 5283 6044 7385 5283
Size: 761 1341 5283
Fragments arranged by size:
5283 1341 761
BclI T'GATC_A
Cuts at: 4905 4905
Size: 7385
BfrBI ATG'CAT
Cuts at: 2221 2221
Size: 7385
BglI GCCn_nnn'nGGC
Cuts at: 773 2711 773
Size: 1938 5447
BglII A'GATC_T
Cuts at: 3212 4900 6937 3212
Size: 1688 2037 3660
Fragments arranged by size:
3660 2037 1688
BlpI GC'TnA_GC
Cuts at: 1297 4497 1297
Size: 3200 4185
Bme1580I G_kGCm'C
Cuts at: 277 417 778 1824 2948 3104 3381 3942 5010
Size: 140 361 1046 1124 156 277 561 1068
Cuts at: 5010 5103 6159 277
Size: 93 1056 1503
Fragments arranged by size:
1503 1124 1068 1056 1046 561 361 277
156 140 93
BmgBI CAC'GTC
Cuts at: 905 2543 2555 2643 905
Size: 1638 12 88 5647
Fragments arranged by size:
5647 1638 88 12
BmrI ACTGGGnnnn_n'
Cuts at: 1194 1472 4597 4678 5013 7157 1194
Size: 278 3125 81 335 2144 1422
Fragments arranged by size:
3125 2144 1422 335 278 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 422 3045 3813 4107 5752 422
Size: 2623 768 294 1645 2055
Fragments arranged by size:
2623 2055 1645 768 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1126 1494 2249 3015 3098 3364 3382 724
Size: 402 368 755 766 83 266 18 4727
Fragments arranged by size:
4727 766 755 402 368 266 83 18
Bpu10I CC'TnA_GC
Cuts at: 500 3853 3959 4142 500
Size: 3353 106 183 3743
Fragments arranged by size:
3743 3353 183 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 2043 4561 5440 5932 6230 832
Size: 1211 2518 879 492 298 1987
Fragments arranged by size:
2518 1987 1211 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2616 7089 2616
Size: 4473 2912
BsaAI yAC'GTr
Cuts at: 1376 4738 1376
Size: 3362 4023
BsaBI GATnn'nnATC
Cuts at: 6 1631 4899 6936 6
Size: 1625 3268 2037 455
Fragments arranged by size:
3268 2037 1625 455
BsaHI Gr'CG_yC
Cuts at: 824 1127 4816 5064 5766 7262 824
Size: 303 3689 248 702 1496 947
Fragments arranged by size:
3689 1496 947 702 303 248
BsaWI w'CCGG_w
Cuts at: 2403 4574 5095 5773 6047 6194 6605 2403
Size: 2171 521 678 274 147 411 3183
Fragments arranged by size:
3183 2171 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1323 1353 2817 2847 3052 3082 4175 4205 1323
Size: 30 1464 30 205 30 1093 30 4503
Fragments arranged by size:
4503 1464 1093 205 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 348 829 1087 1090 1111 1482 1727 1882
Size: 29 481 258 3 21 371 245 155
Cuts at: 1882 3202 3361 3418 3436 3439 3442 3460 3748
Size: 1320 159 57 18 3 3 18 288
Cuts at: 3748 3903 4015 319
Size: 155 112 3689
Fragments arranged by size:
3689 1320 481 371 288 258 245 159
155 155 112 57 29 21 18 18
3 3 3
BseYI C'CCAG_C
Cuts at: 136 374 1842 2685 2727 4810 5568 6145 6641
Size: 238 1468 843 42 2083 758 577 496
Cuts at: 6641 136
Size: 880
Fragments arranged by size:
2083 1468 880 843 758 577 496 238
42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 238 2033 2038 2553 2605 2855 3020 3104
Size: 61 1795 5 515 52 250 165 84
Cuts at: 3104 3329 3376 177
Size: 225 47 4186
Fragments arranged by size:
4186 1795 515 250 225 165 84 61
52 47 5
BsiEI CG_ry'CG
Cuts at: 104 4927 4973 6181 104
Size: 4823 46 1208 1308
Fragments arranged by size:
4823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 417 629 3026 3389 4399 5177 5367 6159 417
Size: 212 2397 363 1010 778 190 792 1643
Fragments arranged by size:
2397 1643 1010 792 778 363 212 190
BsmI GAATG_Cn'
Cuts at: 2475 2475
Size: 7385
Bsp1286I G_dGCh'C
Cuts at: 277 417 629 778 1474 1814 1824 1858 2001
Size: 140 212 149 696 340 10 34 143
Cuts at: 2001 2029 2948 3026 3104 3381 3389 3692 3905
Size: 28 919 78 78 277 8 303 213
Cuts at: 3905 3942 4088 4399 5010 5103 5177 5367 5429
Size: 37 146 311 611 93 74 190 62
Cuts at: 5429 6159 277
Size: 730 1503
Fragments arranged by size:
1503 919 730 696 611 340 311 303
277 213 212 190 149 146 143 140
93 78 78 74 62 37 34 28
10 8
BspEI T'CCGG_A
Cuts at: 6605 6605
Size: 7385
BspHI T'CATG_A
Cuts at: 6561 6561
Size: 7385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 1030 2114 2448 2763 2778 3398 4435 4649
Size: 764 1084 334 315 15 620 1037 214
Cuts at: 4649 4951 5332 266
Size: 302 381 2319
Fragments arranged by size:
2319 1084 1037 764 620 381 334 315
302 214 15
BsrBI CCG'CTC
Cuts at: 50 5677 5731 7215 7380 50
Size: 5627 54 1484 165 55
Fragments arranged by size:
5627 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1991 2436 5297 1991
Size: 445 2861 4079
Fragments arranged by size:
4079 2861 445
BsrFI r'CCGG_y
Cuts at: 385 5383 5564 5773 385
Size: 4998 181 209 1997
Fragments arranged by size:
4998 1997 209 181
BssSI C'ACGA_G
Cuts at: 444 628 5656 6014 444
Size: 184 5028 358 1815
Fragments arranged by size:
5028 1815 358 184
BstAPI GCAn_nnn'nTGC
Cuts at: 2797 3071 4762 2797
Size: 274 1691 5420
Fragments arranged by size:
5420 1691 274
BstBI TT'CG_AA
Cuts at: 5746 5746
Size: 7385
BstXI CCAn_nnnn'nTGG
Cuts at: 2715 2715
Size: 7385
BstYI r'GATC_y
Cuts at: 1 1440 3212 4410 4877 4900 5235 5481 6493
Size: 1439 1772 1198 467 23 335 246 1012
Cuts at: 6493 6579 6591 6937 1
Size: 86 12 346 449
Fragments arranged by size:
1772 1439 1198 1012 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 227 293 2073 2708 227
Size: 66 1780 635 4904
Fragments arranged by size:
4904 1780 635 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1361 5335 5516 5679 1361
Size: 3974 181 163 3067
Fragments arranged by size:
3974 3067 181 163
BtsI GCAGTG_nn'
Cuts at: 396 465 1032 2050 2931 4628 396
Size: 69 567 1018 881 1697 3153
Fragments arranged by size:
3153 1697 1018 881 567 69
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 886 921 886
Size: 35 7350
DraI TTT'AAA
Cuts at: 1197 4384 1197
Size: 3187 4198
DrdI GACnn_nn'nnGTC
Cuts at: 1132 1396 5091 5949 1132
Size: 264 3695 858 2568
Fragments arranged by size:
3695 2568 858 264
EaeI y'GGCC_r
Cuts at: 743 1667 2010 2537 2885 4970 5144 5535 5562
Size: 924 343 527 348 2085 174 391 27
Cuts at: 5562 6907 743
Size: 1345 1221
Fragments arranged by size:
2085 1345 1221 924 527 391 348 343
174 27
EagI C'GGCC_G
Cuts at: 4970 4970
Size: 7385
EarI CTCTTCn'nnn_
Cuts at: 1111 2231 3437 3657 5408 5618 1111
Size: 1120 1206 220 1751 210 2878
Fragments arranged by size:
2878 1751 1206 1120 220 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 502 706 5593 5903 6049 7195 502
Size: 204 4887 310 146 1146 692
Fragments arranged by size:
4887 1146 692 310 204 146
EcoICRI GAG'CTC
Cuts at: 627 4397 627
Size: 3770 3615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 724 946 1126 1494 1744 2249 2615 3015
Size: 311 222 180 368 250 505 366 400
Cuts at: 3015 3098 3364 3382 3463 3472 3656 5210 5642
Size: 83 266 18 81 9 184 1554 432
Cuts at: 5642 6389 6657 413
Size: 747 268 1141
Fragments arranged by size:
1554 1141 747 505 432 400 368 366
311 268 266 250 222 184 180 83
81 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 811 832 2633 3400 3763 811
Size: 21 1801 767 363 4433
Fragments arranged by size:
4433 1801 767 363 21
EcoO109I rG'GnC_Cy
Cuts at: 651 774 1820 1821 3377 4524 651
Size: 123 1046 1 1556 1147 3512
Fragments arranged by size:
3512 1556 1147 1046 123 1
EcoRI G'AATT_C
Cuts at: 3091 4389 3091
Size: 1298 6087
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 364 1358 3180 5404 364
Size: 994 1822 2224 2345
Fragments arranged by size:
2345 2224 1822 994
FspI TGC'GCA
Cuts at: 1084 5166 1084
Size: 4082 3303
HaeII r_GCGC'y
Cuts at: 827 2734 2909 4819 5067 6089 7068 827
Size: 1907 175 1910 248 1022 979 1144
Fragments arranged by size:
1910 1907 1144 1022 979 248 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1549 1581 2850 2882 3001 3033 3199 3231 3910
Size: 32 1269 32 119 32 166 32 679
Cuts at: 3910 3942 3961 3993 4048 4080 4175 4207 4316
Size: 32 19 32 55 32 95 32 109
Cuts at: 4316 4348 5227 5257 5259 5289 1549
Size: 32 879 30 2 30 3645
Fragments arranged by size:
3645 1269 879 679 166 119 109 95
55 32 32 32 32 32 32 32
32 32 30 30 19 2
HincII GTy'rAC
Cuts at: 1137 1175 1226 3237 3735 3969 4014 4194 4424
Size: 38 51 2011 498 234 45 180 230
Cuts at: 4424 1137
Size: 4098
Fragments arranged by size:
4098 2011 498 234 230 180 51 45
38
HindIII A'AGCT_T
Cuts at: 1961 3157 1961
Size: 1196 6189
HpaI GTT'AAC
Cuts at: 1175 1226 1175
Size: 51 7334
KasI G'GCGC_C
Cuts at: 823 4815 5063 823
Size: 3992 248 3145
Fragments arranged by size:
3992 3145 248
KpnI G_GTAC'C
Cuts at: 1062 4405 1062
Size: 3343 4042
MfeI C'AATT_G
Cuts at: 4202 4202
Size: 7385
MluI A'CGCG_T
Cuts at: 3304 6818 3304
Size: 3514 3871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3530 6056 6240 3530
Size: 2526 184 4675
Fragments arranged by size:
4675 2526 184
MscI TGG'CCA
Cuts at: 1669 2012 2539 5146 1669
Size: 343 527 2607 3908
Fragments arranged by size:
3908 2607 527 343
MslI CAynn'nnrTG
Cuts at: 444 2072 2078 5374 5501 444
Size: 1628 6 3296 127 2328
Fragments arranged by size:
3296 2328 1628 127 6
MspA1I CmG'CkG
Cuts at: 507 573 663 732 2951 3014 3118 3474 4041
Size: 66 90 69 2219 63 104 356 567
Cuts at: 4041 4496 4810 5170 6183 6428 507
Size: 455 314 360 1013 245 1464
Fragments arranged by size:
2219 1464 1013 567 455 360 356 314
245 104 90 69 66 63
NaeI GCC'GGC
Cuts at: 387 5566 387
Size: 5179 2206
NarI GG'CG_CC
Cuts at: 824 4816 5064 824
Size: 3992 248 3145
Fragments arranged by size:
3992 3145 248
NcoI C'CATG_G
Cuts at: 227 2073 227
Size: 1846 5539
NdeI CA'TA_TG
Cuts at: 3674 3674
Size: 7385
NgoMIV G'CCGG_C
Cuts at: 385 5564 385
Size: 5179 2206
NsiI A_TGCA'T
Cuts at: 2223 2223
Size: 7385
NspI r_CATG'y
Cuts at: 443 1173 1284 1305 2083 3558 4438 4938 5845
Size: 730 111 21 778 1475 880 500 907
Cuts at: 5845 443
Size: 1983
Fragments arranged by size:
1983 1475 907 880 778 730 500 111
21
PasI CC'CwG_GG
Cuts at: 1816 1816
Size: 7385
PciI A'CATG_T
Cuts at: 1280 2079 5841 1280
Size: 799 3762 2824
Fragments arranged by size:
3762 2824 799
PflMI CCAn_nnn'nTGG
Cuts at: 597 675 597
Size: 78 7307
PfoI T'CCnGG_A
Cuts at: 3398 3398
Size: 7385
PmeI GTTT'AAAC
Cuts at: 4384 4384
Size: 7385
PmlI CAC'GTG
Cuts at: 1376 1376
Size: 7385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6524 6556 6524
Size: 32 7353
PpuMI rG'GwC_Cy
Cuts at: 651 651
Size: 7385
PshAI GACnn'nnGTC
Cuts at: 1355 1355
Size: 7385
PsiI TTA'TAA
Cuts at: 2290 3271 2290
Size: 981 6404
PspOMI G'GGCC_C
Cuts at: 774 1820 3377 3938 774
Size: 1046 1557 561 4221
Fragments arranged by size:
4221 1557 1046 561
PstI C_TGCA'G
Cuts at: 460 512 773 1027 1055 1357 2445 2926 3726
Size: 52 261 254 28 302 1088 481 800
Cuts at: 3726 4432 5117 460
Size: 706 685 2728
Fragments arranged by size:
2728 1088 800 706 685 481 302 261
254 52 28
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7385
PvuII CAG'CTG
Cuts at: 507 663 732 3014 3118 4041 4810 5170 507
Size: 156 69 2282 104 923 769 360 2722
Fragments arranged by size:
2722 2282 923 769 360 156 104 69
RsrII CG'GwC_CG
Cuts at: 489 5580 489
Size: 5091 2294
SacI G_AGCT'C
Cuts at: 629 4399 629
Size: 3770 3615
SalI G'TCGA_C
Cuts at: 1135 3733 3967 4422 1135
Size: 2598 234 455 4098
Fragments arranged by size:
4098 2598 455 234
SanDI GG'GwC_CC
Cuts at: 651 651
Size: 7385
SapI GCTCTTCn'nnn_
Cuts at: 5408 5618 5408
Size: 210 7175
SbfI CC_TGCA'GG
Cuts at: 773 4432 773
Size: 3659 3726
ScaI AGT'ACT
Cuts at: 3070 6902 3070
Size: 3832 3553
SfcI C'TryA_G
Cuts at: 33 456 508 769 1023 1051 1326 1353 2441
Size: 423 52 261 254 28 275 27 1088
Cuts at: 2441 2922 3722 4428 5113 6106 6297 33
Size: 481 800 706 685 993 191 1121
Fragments arranged by size:
1121 1088 993 800 706 685 481 423
275 261 254 191 52 28 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 773 773
Size: 7385
SfoI GGC'GCC
Cuts at: 825 4817 5065 825
Size: 3992 248 3145
Fragments arranged by size:
3992 3145 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7385
SmaI CCC'GGG
Cuts at: 3382 3937 4407 3382
Size: 555 470 6360
Fragments arranged by size:
6360 555 470
SmlI C'TyrA_G
Cuts at: 689 811 2022 4540 5455 5947 6209 689
Size: 122 1211 2518 915 492 262 1865
Fragments arranged by size:
2518 1865 1211 915 492 262 122
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7385
SphI G_CATG'C
Cuts at: 443 1305 4438 4938 443
Size: 862 3133 500 2890
Fragments arranged by size:
3133 2890 862 500
SspI AAT'ATT
Cuts at: 2375 4279 2375
Size: 1904 5481
StuI AGG'CCT
Cuts at: 454 454
Size: 7385
StyI C'CwwG_G
Cuts at: 227 236 746 861 2073 3764 3908 4519 4873
Size: 9 510 115 1212 1691 144 611 354
Cuts at: 4873 227
Size: 2739
Fragments arranged by size:
2739 1691 1212 611 510 354 144 115
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 258 400 4968 5101 5560 5769 258
Size: 142 4568 133 459 209 1874
Fragments arranged by size:
4568 1874 459 209 142 133
TatI w'GTAC_w
Cuts at: 3068 6900 3068
Size: 3832 3553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1323 1355 2294 2326 3649 3681 6902 6934 1323
Size: 32 939 32 1323 32 3221 32 1774
Fragments arranged by size:
3221 1774 1323 939 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3730 3948 4189 5182 3730
Size: 218 241 993 5933
Fragments arranged by size:
5933 993 241 218
XbaI T'CTAG_A
Cuts at: 4416 4416
Size: 7385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4880 4880
Size: 7385
XhoI C'TCGA_G
Cuts at: 689 689
Size: 7385
XmaI C'CCGG_G
Cuts at: 3380 3935 4405 3380
Size: 555 470 6360
Fragments arranged by size:
6360 555 470
XmnI GAAnn'nnTTC
Cuts at: 892 1258 2411 3158 892
Size: 366 1153 747 5119
Fragments arranged by size:
5119 1153 747 366
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AleI AloI AscI AvrII BcgI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI NheI NotI NruI PacI
PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SwaI
ZraI