Restriction map for
pF1KB6987
(Circular) (Six-base) MAPSORT of: pF1KB6987.seq Check: 8387 from: 1 to: 3770
pF1KB6987 3770 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 808 808
Size: 3770
Acc65I G'GTAC_C
Cuts at: 786 786
Size: 3770
AclI AA'CG_TT
Cuts at: 3610 3610
Size: 3770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 563 1595 2027 2774 3042 145
Size: 418 1032 432 747 268 873
Fragments arranged by size:
1032 873 747 432 418 268
AflIII A'CryG_T
Cuts at: 1122 2226 3203 1122
Size: 1104 977 1689
Fragments arranged by size:
1689 1104 977
AgeI A'CCGG_T
Cuts at: 2158 2158
Size: 3770
AlwNI CAG_nnn'CTG
Cuts at: 334 520 1075 2642 334
Size: 186 555 1567 1462
Fragments arranged by size:
1567 1462 555 186
ApaI G_GGCC'C
Cuts at: 555 555
Size: 3770
ApaLI G'TGCA_C
Cuts at: 2540 2540
Size: 3770
ApoI r'AATT_y
Cuts at: 374 774 3123 3379 374
Size: 400 2349 256 765
Fragments arranged by size:
2349 765 400 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
AvaI C'yCGr_G
Cuts at: 144 218 790 144
Size: 74 572 3124
Fragments arranged by size:
3124 572 74
BamHI G'GATC_C
Cuts at: 795 795
Size: 3770
BanI G'GyrC_C
Cuts at: 786 1200 1448 1483 786
Size: 414 248 35 3073
Fragments arranged by size:
3073 414 248 35
BanII G_rGCy'C
Cuts at: 227 512 555 758 784 1814 227
Size: 285 43 203 26 1030 2183
Fragments arranged by size:
2183 1030 285 203 43 26
BbeI G_GCGC'C
Cuts at: 1204 1452 1204
Size: 248 3522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 345 379 345
Size: 34 3736
BciVI GTATCCnnnnn_n'
Cuts at: 1668 2429 3770 1668
Size: 761 1341 1668
Fragments arranged by size:
1668 1341 761
BclI T'GATC_A
Cuts at: 1290 1290
Size: 3770
BglII A'GATC_T
Cuts at: 1285 3322 1285
Size: 2037 1733
BlpI GC'TnA_GC
Cuts at: 882 882
Size: 3770
Bme1580I G_kGCm'C
Cuts at: 555 1395 1488 2544 555
Size: 840 93 1056 1781
Fragments arranged by size:
1781 1056 840 93
BmrI ACTGGGnnnn_n'
Cuts at: 449 527 627 982 1063 1398 3542 449
Size: 78 100 355 81 335 2144 677
Fragments arranged by size:
2144 677 355 335 100 81 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 3770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 670
Size: 3770
Bpu10I CC'TnA_GC
Cuts at: 404 404
Size: 3770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1825 2317 2615 946
Size: 879 492 298 2101
Fragments arranged by size:
2101 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3474 3474
Size: 3770
BsaAI yAC'GTr
Cuts at: 1123 1123
Size: 3770
BsaBI GATnn'nnATC
Cuts at: 6 1284 3321 6
Size: 1278 2037 455
Fragments arranged by size:
2037 1278 455
BsaHI Gr'CG_yC
Cuts at: 1201 1449 2151 3647 1201
Size: 248 702 1496 1324
Fragments arranged by size:
1496 1324 702 248
BsaWI w'CCGG_w
Cuts at: 176 959 1480 2158 2432 2579 2990 176
Size: 783 521 678 274 147 411 956
Fragments arranged by size:
956 783 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 184 452 587 181
Size: 3 268 135 3364
Fragments arranged by size:
3364 268 135 3
BseYI C'CCAG_C
Cuts at: 570 1195 1953 2530 3026 570
Size: 625 758 577 496 1314
Fragments arranged by size:
1314 758 625 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 506
Size: 3770
BsiEI CG_ry'CG
Cuts at: 104 1312 1358 2566 104
Size: 1208 46 1208 1308
Fragments arranged by size:
1308 1208 1208 46
BsiHKAI G_wGCw'C
Cuts at: 227 512 758 784 1562 1752 2544 227
Size: 285 246 26 778 190 792 1453
Fragments arranged by size:
1453 792 778 285 246 190 26
BsmI GAATG_Cn'
Cuts at: 348 348
Size: 3770
Bsp1286I G_dGCh'C
Cuts at: 227 512 555 758 784 1395 1488 1562 1752
Size: 285 43 203 26 611 93 74 190
Cuts at: 1752 1814 2544 227
Size: 62 730 1453
Fragments arranged by size:
1453 730 611 285 203 190 93 74
62 43 26
BspEI T'CCGG_A
Cuts at: 176 2990 176
Size: 2814 956
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 820 1034 1336 1717 820
Size: 214 302 381 2873
Fragments arranged by size:
2873 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2062 2116 3600 3765 50
Size: 2012 54 1484 165 55
Fragments arranged by size:
2012 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 488 1682 488
Size: 1194 2576
BsrFI r'CCGG_y
Cuts at: 1768 1949 2158 1768
Size: 181 209 3380
Fragments arranged by size:
3380 209 181
BssSI C'ACGA_G
Cuts at: 2041 2399 2041
Size: 358 3412
BstAPI GCAn_nnn'nTGC
Cuts at: 334 343 1147 334
Size: 9 804 2957
Fragments arranged by size:
2957 804 9
BstBI TT'CG_AA
Cuts at: 2131 2131
Size: 3770
BstYI r'GATC_y
Cuts at: 1 795 1262 1285 1620 1866 2878 2964 2976
Size: 794 467 23 335 246 1012 86 12
Cuts at: 2976 3322 1
Size: 346 449
Fragments arranged by size:
1012 794 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 647 647
Size: 3770
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1720 1901 2064 1720
Size: 181 163 3426
Fragments arranged by size:
3426 181 163
BtsI GCAGTG_nn'
Cuts at: 622 1013 622
Size: 391 3379
DraI TTT'AAA
Cuts at: 769 769
Size: 3770
DrdI GACnn_nn'nnGTC
Cuts at: 1476 2334 1476
Size: 858 2912
EaeI y'GGCC_r
Cuts at: 422 1355 1529 1920 1947 3292 422
Size: 933 174 391 27 1345 900
Fragments arranged by size:
1345 933 900 391 174 27
EagI C'GGCC_G
Cuts at: 1355 1355
Size: 3770
EarI CTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1978 2288 2434 3580 1978
Size: 310 146 1146 2168
Fragments arranged by size:
2168 1146 310 146
EcoICRI GAG'CTC
Cuts at: 225 510 756 782 225
Size: 285 246 26 3213
Fragments arranged by size:
3213 285 246 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 563 670 1595 2027 2774 3042 145
Size: 418 107 925 432 747 268 873
Fragments arranged by size:
925 873 747 432 418 268 107
EcoNI CCTnn'n_nnAGG
Cuts at: 163 163
Size: 3770
EcoO109I rG'GnC_Cy
Cuts at: 552 909 552
Size: 357 3413
EcoRI G'AATT_C
Cuts at: 774 774
Size: 3770
EcoRV GAT'ATC
Cuts at: 210 210
Size: 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3770
FspI TGC'GCA
Cuts at: 1551 1551
Size: 3770
HaeII r_GCGC'y
Cuts at: 586 1204 1452 2474 3453 586
Size: 618 248 1022 979 903
Fragments arranged by size:
1022 979 903 618 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 336 1612 1642 1644 1674 304
Size: 32 1276 30 2 30 2400
Fragments arranged by size:
2400 1276 32 30 30 2
HincII GTy'rAC
Cuts at: 809 809
Size: 3770
KasI G'GCGC_C
Cuts at: 1200 1448 1200
Size: 248 3522
KpnI G_GTAC'C
Cuts at: 790 790
Size: 3770
MluI A'CGCG_T
Cuts at: 3203 3203
Size: 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2625 2441
Size: 184 3586
MscI TGG'CCA
Cuts at: 424 1531 424
Size: 1107 2663
MslI CAynn'nnrTG
Cuts at: 418 1759 1886 418
Size: 1341 127 2302
Fragments arranged by size:
2302 1341 127
MspA1I CmG'CkG
Cuts at: 574 881 1195 1555 2568 2813 574
Size: 307 314 360 1013 245 1531
Fragments arranged by size:
1531 1013 360 314 307 245
NaeI GCC'GGC
Cuts at: 1951 1951
Size: 3770
NarI GG'CG_CC
Cuts at: 1201 1449 1201
Size: 248 3522
NdeI CA'TA_TG
Cuts at: 504 504
Size: 3770
NgoMIV G'CCGG_C
Cuts at: 1949 1949
Size: 3770
NspI r_CATG'y
Cuts at: 631 823 1323 2230 631
Size: 192 500 907 2171
Fragments arranged by size:
2171 907 500 192
PciI A'CATG_T
Cuts at: 2226 2226
Size: 3770
PmeI GTTT'AAAC
Cuts at: 769 769
Size: 3770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 3738
PsiI TTA'TAA
Cuts at: 271 271
Size: 3770
PspOMI G'GGCC_C
Cuts at: 551 551
Size: 3770
PstI C_TGCA'G
Cuts at: 741 817 1502 741
Size: 76 685 3009
Fragments arranged by size:
3009 685 76
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3770
PvuII CAG'CTG
Cuts at: 1195 1555 1195
Size: 360 3410
RsrII CG'GwC_CG
Cuts at: 1965 1965
Size: 3770
SacI G_AGCT'C
Cuts at: 227 512 758 784 227
Size: 285 246 26 3213
Fragments arranged by size:
3213 285 246 26
SalI G'TCGA_C
Cuts at: 807 807
Size: 3770
SapI GCTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
SbfI CC_TGCA'GG
Cuts at: 817 817
Size: 3770
ScaI AGT'ACT
Cuts at: 459 3287 459
Size: 2828 942
SfcI C'TryA_G
Cuts at: 33 293 737 813 1498 2491 2682 33
Size: 260 444 76 685 993 191 1121
Fragments arranged by size:
1121 993 685 444 260 191 76
SfoI GGC'GCC
Cuts at: 1202 1450 1202
Size: 248 3522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
SmaI CCC'GGG
Cuts at: 792 792
Size: 3770
SmlI C'TyrA_G
Cuts at: 925 1840 2332 2594 925
Size: 915 492 262 2101
Fragments arranged by size:
2101 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3770
SphI G_CATG'C
Cuts at: 631 823 1323 631
Size: 192 500 3078
Fragments arranged by size:
3078 500 192
StyI C'CwwG_G
Cuts at: 904 1258 904
Size: 354 3416
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1353 1486 1945 2154 1353
Size: 133 459 209 2969
Fragments arranged by size:
2969 459 209 133
TatI w'GTAC_w
Cuts at: 457 3285 457
Size: 2828 942
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 3738
Tth111I GACn'n_nGTC
Cuts at: 1567 1567
Size: 3770
XbaI T'CTAG_A
Cuts at: 801 801
Size: 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1265 1265
Size: 3770
XmaI C'CCGG_G
Cuts at: 790 790
Size: 3770
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PmeI PpiI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI DraIII FseI FspAI HindIII
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI