Restriction map for
pF1KB6977
(Circular) (Six-base) MAPSORT of: pF1KB6977.seq Check: 6472 from: 1 to: 3764
pF1KB6977 3764 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AatII G_ACGT'C
Cuts at: 130 130
Size: 3764
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 666 1589 2021 2768 3036 666
Size: 923 432 747 268 1394
Fragments arranged by size:
1394 923 747 432 268
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 439 439
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 1069 2636 1069
Size: 1567 2197
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 784 784
Size: 3764
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 496 780 1194 1442 1477 496
Size: 284 414 248 35 2783
Fragments arranged by size:
2783 414 284 248 35
BanII G_rGCy'C
Cuts at: 452 778 1808 452
Size: 326 1030 2408
Fragments arranged by size:
2408 1030 326
BbeI G_GCGC'C
Cuts at: 500 1198 1446 500
Size: 698 248 2818
Fragments arranged by size:
2818 698 248
BbsI GAAGACnn'nnnn_
Cuts at: 465 465
Size: 3764
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglII A'GATC_T
Cuts at: 596 1279 3316 596
Size: 683 2037 1044
Fragments arranged by size:
2037 1044 683
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 1389 1482 2538 1389
Size: 93 1056 2615
Fragments arranged by size:
2615 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 599 976 1057 1392 3536 599
Size: 377 81 335 2144 827
Fragments arranged by size:
2144 827 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 466
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 940 1819 2311 2609 640
Size: 300 879 492 298 1795
Fragments arranged by size:
1795 879 492 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 127 497 1195 1443 2145 3641 127
Size: 370 698 248 702 1496 250
Fragments arranged by size:
1496 702 698 370 250 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1189 1947 2524 3020 1189
Size: 758 577 496 1933
Fragments arranged by size:
1933 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 475 745 229
Size: 246 270 3248
Fragments arranged by size:
3248 270 246
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 778 1556 1746 2538 778
Size: 778 190 792 2004
Fragments arranged by size:
2004 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 452 778 1389 1482 1556 1746 1808 2538 452
Size: 326 611 93 74 190 62 730 1678
Fragments arranged by size:
1678 730 611 326 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 814 1028 1330 1711 814
Size: 214 302 381 2867
Fragments arranged by size:
2867 381 302 214
BsrBI CCG'CTC
Cuts at: 50 734 2056 2110 3594 3759 50
Size: 684 1322 54 1484 165 55
Fragments arranged by size:
1484 1322 684 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 1762 1943 2152 1762
Size: 181 209 3374
Fragments arranged by size:
3374 209 181
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 1141 1141
Size: 3764
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstXI CCAn_nnnn'nTGG
Cuts at: 486 486
Size: 3764
BstYI r'GATC_y
Cuts at: 1 596 789 1256 1279 1614 1860 2872 2958
Size: 595 193 467 23 335 246 1012 86
Cuts at: 2958 2970 3316 1
Size: 12 346 449
Fragments arranged by size:
1012 595 467 449 346 335 246 193
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 463 463
Size: 3764
BtgI C'CryG_G
Cuts at: 107 107
Size: 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 186 733 1007 186
Size: 547 274 2943
Fragments arranged by size:
2943 547 274
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 511 1470 2328 511
Size: 959 858 1947
Fragments arranged by size:
1947 959 858
EaeI y'GGCC_r
Cuts at: 1349 1523 1914 1941 3286 1349
Size: 174 391 27 1345 1827
Fragments arranged by size:
1827 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2282 2428 3574 1972
Size: 310 146 1146 2162
Fragments arranged by size:
2162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 666 1589 2021 2768 3036 466
Size: 200 923 432 747 268 1194
Fragments arranged by size:
1194 923 747 432 268 200
EcoNI CCTnn'n_nnAGG
Cuts at: 204 372 526 204
Size: 168 154 3442
Fragments arranged by size:
3442 168 154
EcoO109I rG'GnC_Cy
Cuts at: 531 903 531
Size: 372 3392
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 718 1545 718
Size: 827 2937
HaeII r_GCGC'y
Cuts at: 500 1198 1446 2468 3447 500
Size: 698 248 1022 979 817
Fragments arranged by size:
1022 979 817 698 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 616 648 1606 1636 1638 1668 616
Size: 32 958 30 2 30 2712
Fragments arranged by size:
2712 958 32 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 496 1194 1442 496
Size: 698 248 2818
Fragments arranged by size:
2818 698 248
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 385 395 422 2435 2619 385
Size: 10 27 2013 184 1530
Fragments arranged by size:
2013 1530 184 27 10
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 641 1753 1880 641
Size: 1112 127 2525
Fragments arranged by size:
2525 1112 127
MspA1I CmG'CkG
Cuts at: 252 749 875 1189 1549 2562 2807 252
Size: 497 126 314 360 1013 245 1209
Fragments arranged by size:
1209 1013 497 360 314 245 126
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 497 1195 1443 497
Size: 698 248 2818
Fragments arranged by size:
2818 698 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PfoI T'CCnGG_A
Cuts at: 121 121
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PpuMI rG'GwC_Cy
Cuts at: 531 531
Size: 3764
PstI C_TGCA'G
Cuts at: 323 811 1496 323
Size: 488 685 2591
Fragments arranged by size:
2591 685 488
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 252 749 1189 1549 252
Size: 497 440 360 2467
Fragments arranged by size:
2467 497 440 360
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 113 319 612 807 1492 2485 2676 33
Size: 80 206 293 195 685 993 191 1121
Fragments arranged by size:
1121 993 685 293 206 195 191 80
SfoI GGC'GCC
Cuts at: 498 1196 1444 498
Size: 698 248 2818
Fragments arranged by size:
2818 698 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 786 786
Size: 3764
SmlI C'TyrA_G
Cuts at: 655 919 1834 2326 2588 655
Size: 264 915 492 262 1831
Fragments arranged by size:
1831 915 492 264 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 107 898 1252 107
Size: 791 354 2619
Fragments arranged by size:
2619 791 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 1480 1939 2148 1347
Size: 133 459 209 2963
Fragments arranged by size:
2963 459 209 133
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 700 1561 700
Size: 861 2903
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XmaI C'CCGG_G
Cuts at: 784 784
Size: 3764
ZraI GAC'GTC
Cuts at: 128 128
Size: 3764
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI