Restriction map for
pF1KB6974
(Circular) (Six-base) MAPSORT of: pF1KB6974.seq Check: 4527 from: 1 to: 3842
pF1KB6974 3842 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 223 223
Size: 3842
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 1667 2099 2846 3114 401
Size: 1266 432 747 268 1129
Fragments arranged by size:
1266 1129 747 432 268
AflIII A'CryG_T
Cuts at: 1194 2298 3275 1194
Size: 1104 977 1761
Fragments arranged by size:
1761 1104 977
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 1147 2714 1147
Size: 1567 2275
ApaLI G'TGCA_C
Cuts at: 2612 2612
Size: 3842
ApoI r'AATT_y
Cuts at: 555 846 3195 3451 555
Size: 291 2349 256 946
Fragments arranged by size:
2349 946 291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 862 862
Size: 3842
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 527 560 527
Size: 33 3809
BamHI G'GATC_C
Cuts at: 867 867
Size: 3842
BanI G'GyrC_C
Cuts at: 858 1272 1520 1555 858
Size: 414 248 35 3145
Fragments arranged by size:
3145 414 248 35
BanII G_rGCy'C
Cuts at: 856 1886 856
Size: 1030 2812
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 1467 1560 2616 1467
Size: 93 1056 2693
Fragments arranged by size:
2693 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1054 1135 1470 3614 1054
Size: 81 335 2144 1282
Fragments arranged by size:
2144 1282 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 843 2209 843
Size: 1366 2476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 172 251 524 796 163
Size: 9 79 273 272 3209
Fragments arranged by size:
3209 273 272 79 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1018 1897 2389 2687 247
Size: 771 879 492 298 1402
Fragments arranged by size:
1402 879 771 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3546 3546
Size: 3842
BsaAI yAC'GTr
Cuts at: 1195 1195
Size: 3842
BsaBI GATnn'nnATC
Cuts at: 6 680 1356 3393 6
Size: 674 676 2037 455
Fragments arranged by size:
2037 676 674 455
BsaHI Gr'CG_yC
Cuts at: 220 1273 1521 2223 3719 220
Size: 1053 248 702 1496 343
Fragments arranged by size:
1496 1053 702 343 248
BsaWI w'CCGG_w
Cuts at: 1031 1552 2230 2504 2651 3062 1031
Size: 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 154
Size: 30 3812
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 139
Size: 3842
BseYI C'CCAG_C
Cuts at: 199 1267 2025 2602 3098 199
Size: 1068 758 577 496 943
Fragments arranged by size:
1068 943 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1384 1430 2638 104
Size: 1280 46 1208 1308
Fragments arranged by size:
1308 1280 1208 46
BsiHKAI G_wGCw'C
Cuts at: 856 1634 1824 2616 856
Size: 778 190 792 2082
Fragments arranged by size:
2082 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 856 1467 1560 1634 1824 1886 2616 856
Size: 611 93 74 190 62 730 2082
Fragments arranged by size:
2082 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 3018 3018
Size: 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1106 1408 1789 892
Size: 214 302 381 2945
Fragments arranged by size:
2945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2134 2188 3672 3837 50
Size: 2084 54 1484 165 55
Fragments arranged by size:
2084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1754 1754
Size: 3842
BsrFI r'CCGG_y
Cuts at: 1840 2021 2230 1840
Size: 181 209 3452
Fragments arranged by size:
3452 209 181
BssSI C'ACGA_G
Cuts at: 2113 2471 2113
Size: 358 3484
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstYI r'GATC_y
Cuts at: 1 867 1334 1357 1692 1938 2950 3036 3048
Size: 866 467 23 335 246 1012 86 12
Cuts at: 3048 3394 1
Size: 346 449
Fragments arranged by size:
1012 866 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 531 531
Size: 3842
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1792 1973 2136 1792
Size: 181 163 3498
Fragments arranged by size:
3498 181 163
BtsI GCAGTG_nn'
Cuts at: 1085 1085
Size: 3842
DraI TTT'AAA
Cuts at: 359 841 359
Size: 482 3360
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 1427 1601 1992 2019 3364 1427
Size: 174 391 27 1345 1905
Fragments arranged by size:
1905 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1427 1427
Size: 3842
EarI CTCTTCn'nnn_
Cuts at: 465 1865 2075 465
Size: 1400 210 2232
Fragments arranged by size:
2232 1400 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2050 2360 2506 3652 2050
Size: 310 146 1146 2240
Fragments arranged by size:
2240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 854 854
Size: 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 172 251 401 524 796 1667 2099 2846
Size: 9 79 150 123 272 871 432 747
Cuts at: 2846 3114 163
Size: 268 891
Fragments arranged by size:
891 871 747 432 272 268 150 123
79 9
EcoO109I rG'GnC_Cy
Cuts at: 147 981 147
Size: 834 3008
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
EcoRV GAT'ATC
Cuts at: 699 699
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 319 406 1861 319
Size: 87 1455 2300
Fragments arranged by size:
2300 1455 87
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 1276 1524 2546 3525 1276
Size: 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 396 428 1684 1714 1716 1746 396
Size: 32 1256 30 2 30 2492
Fragments arranged by size:
2492 1256 32 30 30 2
HincII GTy'rAC
Cuts at: 881 881
Size: 3842
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MfeI C'AATT_G
Cuts at: 548 548
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2697 2513
Size: 184 3658
MscI TGG'CCA
Cuts at: 1603 1603
Size: 3842
MslI CAynn'nnrTG
Cuts at: 214 1831 1958 214
Size: 1617 127 2098
Fragments arranged by size:
2098 1617 127
MspA1I CmG'CkG
Cuts at: 424 832 953 1267 1627 2640 2885 424
Size: 408 121 314 360 1013 245 1381
Fragments arranged by size:
1381 1013 408 360 314 245 121
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 3842
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NcoI C'CATG_G
Cuts at: 531 531
Size: 3842
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 3842
NspI r_CATG'y
Cuts at: 895 1395 2302 895
Size: 500 907 2435
Fragments arranged by size:
2435 907 500
PciI A'CATG_T
Cuts at: 2298 2298
Size: 3842
PflMI CCAn_nnn'nTGG
Cuts at: 708 759 708
Size: 51 3791
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 733 765 2981 3013 733
Size: 32 2216 32 1562
Fragments arranged by size:
2216 1562 32 32
PstI C_TGCA'G
Cuts at: 889 1574 889
Size: 685 3157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 424 832 1267 1627 424
Size: 408 435 360 2639
Fragments arranged by size:
2639 435 408 360
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 856 856
Size: 3842
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SfcI C'TryA_G
Cuts at: 33 885 1570 2563 2754 33
Size: 852 685 993 191 1121
Fragments arranged by size:
1121 993 852 685 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 262 997 1912 2404 2666 262
Size: 735 915 492 262 1438
Fragments arranged by size:
1438 915 735 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
SspI AAT'ATT
Cuts at: 314 314
Size: 3842
StyI C'CwwG_G
Cuts at: 531 976 1330 531
Size: 445 354 3043
Fragments arranged by size:
3043 445 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 213 1425 1558 2017 2226 213
Size: 1212 133 459 209 1829
Fragments arranged by size:
1829 1212 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 713 745 3359 3391 713
Size: 32 2614 32 1164
Fragments arranged by size:
2614 1164 32 32
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 330 873 330
Size: 543 3299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1337 1337
Size: 3842
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
ZraI GAC'GTC
Cuts at: 221 221
Size: 3842
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI