Restriction map for
pF1KB6968
(Circular) (Six-base) MAPSORT of: pF1KB6968.seq Check: 4957 from: 1 to: 3836
pF1KB6968 3836 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1661 2093 2840 3108 1661
Size: 432 747 268 2389
Fragments arranged by size:
2389 747 432 268
AflIII A'CryG_T
Cuts at: 1188 2292 3269 1188
Size: 1104 977 1755
Fragments arranged by size:
1755 1104 977
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AlwNI CAG_nnn'CTG
Cuts at: 730 1141 2708 730
Size: 411 1567 1858
Fragments arranged by size:
1858 1567 411
ApaLI G'TGCA_C
Cuts at: 2606 2606
Size: 3836
ApoI r'AATT_y
Cuts at: 111 840 3189 3445 111
Size: 729 2349 256 502
Fragments arranged by size:
2349 729 502 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 856 856
Size: 3836
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 852 1266 1514 1549 852
Size: 414 248 35 3139
Fragments arranged by size:
3139 414 248 35
BanII G_rGCy'C
Cuts at: 850 1880 850
Size: 1030 2806
BbeI G_GCGC'C
Cuts at: 1270 1518 1270
Size: 248 3588
BciVI GTATCCnnnnn_n'
Cuts at: 366 1734 2495 3836 366
Size: 1368 761 1341 366
Fragments arranged by size:
1368 1341 761 366
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 948 948
Size: 3836
Bme1580I G_kGCm'C
Cuts at: 1461 1554 2610 1461
Size: 93 1056 2687
Fragments arranged by size:
2687 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 398 473 1048 1129 1464 3608 398
Size: 75 575 81 335 2144 626
Fragments arranged by size:
2144 626 575 335 81 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1012 1891 2383 2681 502
Size: 510 879 492 298 1657
Fragments arranged by size:
1657 879 510 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 121 3540 121
Size: 3419 417
BsaAI yAC'GTr
Cuts at: 1189 1189
Size: 3836
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 1267 1515 2217 3713 1267
Size: 248 702 1496 1390
Fragments arranged by size:
1496 1390 702 248
BsaWI w'CCGG_w
Cuts at: 1025 1546 2224 2498 2645 3056 1025
Size: 521 678 274 147 411 1805
Fragments arranged by size:
1805 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 290 320 290
Size: 30 3806
BseYI C'CCAG_C
Cuts at: 1261 2019 2596 3092 1261
Size: 758 577 496 2005
Fragments arranged by size:
2005 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1378 1424 2632 104
Size: 1274 46 1208 1308
Fragments arranged by size:
1308 1274 1208 46
BsiHKAI G_wGCw'C
Cuts at: 850 1628 1818 2610 850
Size: 778 190 792 2076
Fragments arranged by size:
2076 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 850 1461 1554 1628 1818 1880 2610 850
Size: 611 93 74 190 62 730 2076
Fragments arranged by size:
2076 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3056 3056
Size: 3836
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 511 886 1100 1402 1783 511
Size: 375 214 302 381 2564
Fragments arranged by size:
2564 381 375 302 214
BsrBI CCG'CTC
Cuts at: 50 2128 2182 3666 3831 50
Size: 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1748 1748
Size: 3836
BsrFI r'CCGG_y
Cuts at: 1834 2015 2224 1834
Size: 181 209 3446
Fragments arranged by size:
3446 209 181
BssSI C'ACGA_G
Cuts at: 2107 2465 2107
Size: 358 3478
BstAPI GCAn_nnn'nTGC
Cuts at: 1213 1213
Size: 3836
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstYI r'GATC_y
Cuts at: 1 861 1328 1351 1686 1932 2944 3030 3042
Size: 860 467 23 335 246 1012 86 12
Cuts at: 3042 3388 1
Size: 346 449
Fragments arranged by size:
1012 860 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 131 131
Size: 3836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1079
Size: 3836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 495 530 495
Size: 35 3801
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 1542 2400 1542
Size: 858 2978
EaeI y'GGCC_r
Cuts at: 324 1421 1595 1986 2013 3358 324
Size: 1097 174 391 27 1345 802
Fragments arranged by size:
1345 1097 802 391 174 27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 425 587 599 1859 2069 425
Size: 162 12 1260 210 2192
Fragments arranged by size:
2192 1260 210 162 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 453 2044 2354 2500 3646 453
Size: 1591 310 146 1146 643
Fragments arranged by size:
1591 1146 643 310 146
EcoICRI GAG'CTC
Cuts at: 848 848
Size: 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1661 2093 2840 3108 1661
Size: 432 747 268 2389
Fragments arranged by size:
2389 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 197 975 197
Size: 778 3058
EcoRI G'AATT_C
Cuts at: 111 840 111
Size: 729 3107
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 668 1855 668
Size: 1187 2649
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 1270 1518 2540 3519 1270
Size: 248 1022 979 1587
Fragments arranged by size:
1587 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 598 630 688 720 1678 1708 1710
Size: 32 304 32 58 32 958 30 2
Cuts at: 1710 1740 262
Size: 30 2358
Fragments arranged by size:
2358 958 304 58 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 875 875
Size: 3836
HindIII A'AGCT_T
Cuts at: 316 316
Size: 3836
KasI G'GCGC_C
Cuts at: 1266 1514 1266
Size: 248 3588
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 202 276 2507 2691 202
Size: 74 2231 184 1347
Fragments arranged by size:
2231 1347 184 74
MscI TGG'CCA
Cuts at: 1597 1597
Size: 3836
MslI CAynn'nnrTG
Cuts at: 1825 1952 1825
Size: 127 3709
MspA1I CmG'CkG
Cuts at: 947 1261 1621 2634 2879 947
Size: 314 360 1013 245 1904
Fragments arranged by size:
1904 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 3836
NarI GG'CG_CC
Cuts at: 1267 1515 1267
Size: 248 3588
NcoI C'CATG_G
Cuts at: 131 131
Size: 3836
NdeI CA'TA_TG
Cuts at: 251 251
Size: 3836
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 3836
NspI r_CATG'y
Cuts at: 889 1389 2296 889
Size: 500 907 2429
Fragments arranged by size:
2429 907 500
PciI A'CATG_T
Cuts at: 2292 2292
Size: 3836
PfoI T'CCnGG_A
Cuts at: 452 452
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PstI C_TGCA'G
Cuts at: 883 1568 883
Size: 685 3151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 1261 1621 1261
Size: 360 3476
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 850 850
Size: 3836
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SexAI A'CCwGG_T
Cuts at: 555 555
Size: 3836
SfcI C'TryA_G
Cuts at: 33 879 1564 2557 2748 33
Size: 846 685 993 191 1121
Fragments arranged by size:
1121 993 846 685 191
SfoI GGC'GCC
Cuts at: 1268 1516 1268
Size: 248 3588
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 481 991 1906 2398 2660 481
Size: 510 915 492 262 1657
Fragments arranged by size:
1657 915 510 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StuI AGG'CCT
Cuts at: 123 123
Size: 3836
StyI C'CwwG_G
Cuts at: 131 165 970 1324 131
Size: 34 805 354 2643
Fragments arranged by size:
2643 805 354 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 300 1419 1552 2011 2220 300
Size: 1119 133 459 209 1916
Fragments arranged by size:
1916 1119 459 209 133
TatI w'GTAC_w
Cuts at: 3351 3351
Size: 3836
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 290 322 3353 3385 290
Size: 32 3031 32 741
Fragments arranged by size:
3031 741 32 32
Tth111I GACn'n_nGTC
Cuts at: 1633 1633
Size: 3836
XbaI T'CTAG_A
Cuts at: 655 867 655
Size: 212 3624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 460 1331 460
Size: 871 2965
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI