Restriction map for
pF1KB6959
(Circular) (Six-base) MAPSORT of: pF1KB6959.seq Check: 4620 from: 1 to: 3752
pF1KB6959 3752 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 326 768 326
Size: 442 3310
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 470 1577 2009 2756 3024 448
Size: 22 1107 432 747 268 1176
Fragments arranged by size:
1176 1107 747 432 268 22
AflIII A'CryG_T
Cuts at: 1104 2208 3185 1104
Size: 1104 977 1671
Fragments arranged by size:
1671 1104 977
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 1057 2624 1057
Size: 1567 2185
ApaI G_GGCC'C
Cuts at: 372 372
Size: 3752
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3752
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 503 772 503
Size: 269 3483
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 277 326 768 1182 1430 1465 277
Size: 49 442 414 248 35 2564
Fragments arranged by size:
2564 442 414 248 49 35
BanII G_rGCy'C
Cuts at: 372 437 766 1796 372
Size: 65 329 1030 2328
Fragments arranged by size:
2328 1030 329 65
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BbsI GAAGACnn'nnnn_
Cuts at: 135 187 135
Size: 52 3700
BbvCI CC'TCA_GC
Cuts at: 493 570 493
Size: 77 3675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 222 256 726 760 222
Size: 34 470 34 3214
Fragments arranged by size:
3214 470 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1650 2411 3752 1650
Size: 761 1341 1650
Fragments arranged by size:
1650 1341 761
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglII A'GATC_T
Cuts at: 299 1267 3304 299
Size: 968 2037 747
Fragments arranged by size:
2037 968 747
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 280 372 515 693 1377 1470 2526 280
Size: 92 143 178 684 93 1056 1506
Fragments arranged by size:
1506 1056 684 178 143 93 92
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BmtI G_CTAG'C
Cuts at: 601 601
Size: 3752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 583 340
Size: 243 3509
Bpu10I CC'TnA_GC
Cuts at: 493 570 493
Size: 77 3675
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1807 2299 2597 928
Size: 879 492 298 2083
Fragments arranged by size:
2083 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 559 3456 559
Size: 2897 855
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 1266 3303 6
Size: 1260 2037 455
Fragments arranged by size:
2037 1260 455
BsaHI Gr'CG_yC
Cuts at: 1183 1431 2133 3629 1183
Size: 248 702 1496 1306
Fragments arranged by size:
1496 1306 702 248
BsaWI w'CCGG_w
Cuts at: 662 941 1462 2140 2414 2561 2972 662
Size: 279 521 678 274 147 411 1442
Fragments arranged by size:
1442 678 521 411 279 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 535 535
Size: 3752
BseYI C'CCAG_C
Cuts at: 1177 1935 2512 3008 1177
Size: 758 577 496 1921
Fragments arranged by size:
1921 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 380
Size: 3752
BsiEI CG_ry'CG
Cuts at: 104 461 1294 1340 2548 104
Size: 357 833 46 1208 1308
Fragments arranged by size:
1308 1208 833 357 46
BsiHKAI G_wGCw'C
Cuts at: 437 766 1544 1734 2526 437
Size: 329 778 190 792 1663
Fragments arranged by size:
1663 792 778 329 190
Bsp1286I G_dGCh'C
Cuts at: 280 372 437 515 693 766 1377 1470 1544
Size: 92 65 78 178 73 611 93 74
Cuts at: 1544 1734 1796 2526 280
Size: 190 62 730 1506
Fragments arranged by size:
1506 730 611 190 178 93 92 78
74 73 65 62
BspEI T'CCGG_A
Cuts at: 662 2972 662
Size: 2310 1442
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 802 1016 1318 1699 802
Size: 214 302 381 2855
Fragments arranged by size:
2855 381 302 214
BsrBI CCG'CTC
Cuts at: 50 324 2044 2098 3582 3747 50
Size: 274 1720 54 1484 165 55
Fragments arranged by size:
1720 1484 274 165 55 54
BsrDI GCAATG_nn'
Cuts at: 216 1664 216
Size: 1448 2304
BsrFI r'CCGG_y
Cuts at: 1750 1931 2140 1750
Size: 181 209 3362
Fragments arranged by size:
3362 209 181
BssSI C'ACGA_G
Cuts at: 472 2023 2381 472
Size: 1551 358 1843
Fragments arranged by size:
1843 1551 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1129
Size: 3752
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstXI CCAn_nnnn'nTGG
Cuts at: 681 681
Size: 3752
BstYI r'GATC_y
Cuts at: 1 299 777 1244 1267 1602 1848 2860 2946
Size: 298 478 467 23 335 246 1012 86
Cuts at: 2946 2958 3304 1
Size: 12 346 449
Fragments arranged by size:
1012 478 467 449 346 335 298 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 435 1702 1883 2046 435
Size: 1267 181 163 2141
Fragments arranged by size:
2141 1267 181 163
BtsI GCAGTG_nn'
Cuts at: 368 693 995 368
Size: 325 302 3125
Fragments arranged by size:
3125 325 302
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 1337 1511 1902 1929 3274 1337
Size: 174 391 27 1345 1815
Fragments arranged by size:
1815 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1337 1337
Size: 3752
EarI CTCTTCn'nnn_
Cuts at: 562 718 1775 1985 562
Size: 156 1057 210 2329
Fragments arranged by size:
2329 1057 210 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1960 2270 2416 3562 1960
Size: 310 146 1146 2150
Fragments arranged by size:
2150 1146 310 146
EcoICRI GAG'CTC
Cuts at: 435 764 435
Size: 329 3423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 448 470 583 1577 2009 2756 3024 340
Size: 108 22 113 994 432 747 268 1068
Fragments arranged by size:
1068 994 747 432 268 113 108 22
EcoNI CCTnn'n_nnAGG
Cuts at: 496 496
Size: 3752
EcoO109I rG'GnC_Cy
Cuts at: 368 369 728 891 368
Size: 1 359 163 3229
Fragments arranged by size:
3229 359 163 1
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 1186 1434 2456 3435 1186
Size: 248 1022 979 1503
Fragments arranged by size:
1503 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1594 1624 1626 1656 1594
Size: 30 2 30 3690
Fragments arranged by size:
3690 30 30 2
HincII GTy'rAC
Cuts at: 791 791
Size: 3752
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 330 772 330
Size: 442 3310
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 268 472 1741 1868 268
Size: 204 1269 127 2152
Fragments arranged by size:
2152 1269 204 127
MspA1I CmG'CkG
Cuts at: 283 313 863 1177 1537 2550 2795 283
Size: 30 550 314 360 1013 245 1240
Fragments arranged by size:
1240 1013 550 360 314 245 30
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NcoI C'CATG_G
Cuts at: 107 107
Size: 3752
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NheI G'CTAG_C
Cuts at: 597 597
Size: 3752
NspI r_CATG'y
Cuts at: 805 1305 2212 805
Size: 500 907 2345
Fragments arranged by size:
2345 907 500
PasI CC'CwG_GG
Cuts at: 715 715
Size: 3752
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PfoI T'CCnGG_A
Cuts at: 560 560
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PsiI TTA'TAA
Cuts at: 134 134
Size: 3752
PspOMI G'GGCC_C
Cuts at: 368 368
Size: 3752
PstI C_TGCA'G
Cuts at: 799 1484 799
Size: 685 3067
PvuI CG_AT'CG
Cuts at: 104 461 104
Size: 357 3395
PvuII CAG'CTG
Cuts at: 313 1177 1537 313
Size: 864 360 2528
Fragments arranged by size:
2528 864 360
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 437 766 437
Size: 329 3423
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 562 1775 1985 562
Size: 1213 210 2329
Fragments arranged by size:
2329 1213 210
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 795 1480 2473 2664 33
Size: 762 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 191
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 505 774 505
Size: 269 3483
SmlI C'TyrA_G
Cuts at: 907 1822 2314 2576 907
Size: 915 492 262 2083
Fragments arranged by size:
2083 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StyI C'CwwG_G
Cuts at: 107 886 1240 107
Size: 779 354 2619
Fragments arranged by size:
2619 779 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 1468 1927 2136 1335
Size: 133 459 209 2951
Fragments arranged by size:
2951 459 209 133
TatI w'GTAC_w
Cuts at: 3267 3267
Size: 3752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 1247
Size: 3752
XmaI C'CCGG_G
Cuts at: 503 772 503
Size: 269 3483
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PfoI
PmeI PpiI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BglI BmgBI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PflMI PmlI PpuMI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI