Restriction map for
pF1KB6940
(Circular) (Six-base) MAPSORT of: pF1KB6940.seq Check: 8349 from: 1 to: 3800
pF1KB6940 3800 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 442 442
Size: 3800
AccI GT'mk_AC
Cuts at: 838 838
Size: 3800
Acc65I G'GTAC_C
Cuts at: 816 816
Size: 3800
AclI AA'CG_TT
Cuts at: 3640 3640
Size: 3800
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1625 2057 2804 3072 1625
Size: 432 747 268 2353
Fragments arranged by size:
2353 747 432 268
AflIII A'CryG_T
Cuts at: 345 677 1152 2256 3233 345
Size: 332 475 1104 977 912
Fragments arranged by size:
1104 977 912 475 332
AgeI A'CCGG_T
Cuts at: 2188 2188
Size: 3800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 738 748 738
Size: 10 3790
AlwNI CAG_nnn'CTG
Cuts at: 624 1105 2672 624
Size: 481 1567 1752
Fragments arranged by size:
1752 1567 481
ApaLI G'TGCA_C
Cuts at: 646 2570 646
Size: 1924 1876
ApoI r'AATT_y
Cuts at: 315 804 3153 3409 315
Size: 489 2349 256 706
Fragments arranged by size:
2349 706 489 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3800
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
AvaI C'yCGr_G
Cuts at: 530 820 530
Size: 290 3510
BamHI G'GATC_C
Cuts at: 825 825
Size: 3800
BanI G'GyrC_C
Cuts at: 658 816 1230 1478 1513 658
Size: 158 414 248 35 2945
Fragments arranged by size:
2945 414 248 158 35
BanII G_rGCy'C
Cuts at: 814 1844 814
Size: 1030 2770
BbeI G_GCGC'C
Cuts at: 1234 1482 1234
Size: 248 3552
BciVI GTATCCnnnnn_n'
Cuts at: 621 1698 2459 3800 621
Size: 1077 761 1341 621
Fragments arranged by size:
1341 1077 761 621
BclI T'GATC_A
Cuts at: 743 1320 743
Size: 577 3223
BglII A'GATC_T
Cuts at: 719 1315 3352 719
Size: 596 2037 1167
Fragments arranged by size:
2037 1167 596
BlpI GC'TnA_GC
Cuts at: 912 912
Size: 3800
Bme1580I G_kGCm'C
Cuts at: 650 1425 1518 2574 650
Size: 775 93 1056 1876
Fragments arranged by size:
1876 1056 775 93
BmrI ACTGGGnnnn_n'
Cuts at: 1012 1093 1428 3572 1012
Size: 81 335 2144 1240
Fragments arranged by size:
2144 1240 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 3800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 397
Size: 3800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 976 1855 2347 2645 571
Size: 405 879 492 298 1726
Fragments arranged by size:
1726 879 492 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 3504 3504
Size: 3800
BsaAI yAC'GTr
Cuts at: 1153 1153
Size: 3800
BsaBI GATnn'nnATC
Cuts at: 6 1314 3351 6
Size: 1308 2037 455
Fragments arranged by size:
2037 1308 455
BsaHI Gr'CG_yC
Cuts at: 1231 1479 2181 3677 1231
Size: 248 702 1496 1354
Fragments arranged by size:
1496 1354 702 248
BsaWI w'CCGG_w
Cuts at: 989 1510 2188 2462 2609 3020 989
Size: 521 678 274 147 411 1769
Fragments arranged by size:
1769 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 574 787 574
Size: 213 3587
BseYI C'CCAG_C
Cuts at: 129 1225 1983 2560 3056 129
Size: 1096 758 577 496 873
Fragments arranged by size:
1096 873 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 637 277
Size: 360 3440
BsiEI CG_ry'CG
Cuts at: 104 1342 1388 2596 104
Size: 1238 46 1208 1308
Fragments arranged by size:
1308 1238 1208 46
BsiHKAI G_wGCw'C
Cuts at: 650 814 1592 1782 2574 650
Size: 164 778 190 792 1876
Fragments arranged by size:
1876 792 778 190 164
Bsp1286I G_dGCh'C
Cuts at: 650 814 1425 1518 1592 1782 1844 2574 650
Size: 164 611 93 74 190 62 730 1876
Fragments arranged by size:
1876 730 611 190 164 93 74 62
BspEI T'CCGG_A
Cuts at: 3020 3020
Size: 3800
BspHI T'CATG_A
Cuts at: 2976 2976
Size: 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 442 850 1064 1366 1747 442
Size: 408 214 302 381 2495
Fragments arranged by size:
2495 408 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2092 2146 3630 3795 50
Size: 2042 54 1484 165 55
Fragments arranged by size:
2042 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 788 1712 788
Size: 924 2876
BsrFI r'CCGG_y
Cuts at: 1798 1979 2188 1798
Size: 181 209 3410
Fragments arranged by size:
3410 209 181
BsrGI T'GTAC_A
Cuts at: 471 471
Size: 3800
BssSI C'ACGA_G
Cuts at: 2071 2429 2071
Size: 358 3442
BstAPI GCAn_nnn'nTGC
Cuts at: 264 1177 264
Size: 913 2887
BstBI TT'CG_AA
Cuts at: 2161 2161
Size: 3800
BstYI r'GATC_y
Cuts at: 1 719 825 1292 1315 1650 1896 2908 2994
Size: 718 106 467 23 335 246 1012 86
Cuts at: 2994 3006 3352 1
Size: 12 346 449
Fragments arranged by size:
1012 718 467 449 346 335 246 106
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 392 770 392
Size: 378 3422
BtgI C'CryG_G
Cuts at: 251 251
Size: 3800
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1750 1931 2094 1750
Size: 181 163 3456
Fragments arranged by size:
3456 181 163
BtsI GCAGTG_nn'
Cuts at: 1043 1043
Size: 3800
DraI TTT'AAA
Cuts at: 799 799
Size: 3800
DrdI GACnn_nn'nnGTC
Cuts at: 1506 2364 1506
Size: 858 2942
EaeI y'GGCC_r
Cuts at: 1385 1559 1950 1977 3322 1385
Size: 174 391 27 1345 1863
Fragments arranged by size:
1863 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1385 1385
Size: 3800
EarI CTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2008 2318 2464 3610 2008
Size: 310 146 1146 2198
Fragments arranged by size:
2198 1146 310 146
EcoICRI GAG'CTC
Cuts at: 812 812
Size: 3800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1625 2057 2804 3072 397
Size: 1228 432 747 268 1125
Fragments arranged by size:
1228 1125 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 939 939
Size: 3800
EcoRI G'AATT_C
Cuts at: 804 804
Size: 3800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3800
FspI TGC'GCA
Cuts at: 1581 1581
Size: 3800
HaeII r_GCGC'y
Cuts at: 1234 1482 2504 3483 1234
Size: 248 1022 979 1551
Fragments arranged by size:
1551 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1642 1672 1674 1704 1642
Size: 30 2 30 3738
Fragments arranged by size:
3738 30 30 2
HincII GTy'rAC
Cuts at: 839 839
Size: 3800
HindIII A'AGCT_T
Cuts at: 604 604
Size: 3800
KasI G'GCGC_C
Cuts at: 1230 1478 1230
Size: 248 3552
KpnI G_GTAC'C
Cuts at: 820 820
Size: 3800
MluI A'CGCG_T
Cuts at: 3233 3233
Size: 3800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 737 2471 2655 737
Size: 1734 184 1882
Fragments arranged by size:
1882 1734 184
MscI TGG'CCA
Cuts at: 1561 1561
Size: 3800
MslI CAynn'nnrTG
Cuts at: 1789 1916 1789
Size: 127 3673
MspA1I CmG'CkG
Cuts at: 911 1225 1585 2598 2843 911
Size: 314 360 1013 245 1868
Fragments arranged by size:
1868 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1981 1981
Size: 3800
NarI GG'CG_CC
Cuts at: 1231 1479 1231
Size: 248 3552
NcoI C'CATG_G
Cuts at: 251 251
Size: 3800
NgoMIV G'CCGG_C
Cuts at: 1979 1979
Size: 3800
NspI r_CATG'y
Cuts at: 148 349 681 853 1353 2260 148
Size: 201 332 172 500 907 1688
Fragments arranged by size:
1688 907 500 332 201 172
PciI A'CATG_T
Cuts at: 345 677 2256 345
Size: 332 1579 1889
Fragments arranged by size:
1889 1579 332
PfoI T'CCnGG_A
Cuts at: 623 623
Size: 3800
PmeI GTTT'AAAC
Cuts at: 799 799
Size: 3800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 3768
PspXI vC'TCGA_Gb
Cuts at: 530 530
Size: 3800
PstI C_TGCA'G
Cuts at: 847 1532 847
Size: 685 3115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3800
PvuII CAG'CTG
Cuts at: 1225 1585 1225
Size: 360 3440
RsrII CG'GwC_CG
Cuts at: 1995 1995
Size: 3800
SacI G_AGCT'C
Cuts at: 814 814
Size: 3800
SalI G'TCGA_C
Cuts at: 837 837
Size: 3800
SapI GCTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
SbfI CC_TGCA'GG
Cuts at: 847 847
Size: 3800
ScaI AGT'ACT
Cuts at: 3317 3317
Size: 3800
SfcI C'TryA_G
Cuts at: 33 843 1528 2521 2712 33
Size: 810 685 993 191 1121
Fragments arranged by size:
1121 993 810 685 191
SfoI GGC'GCC
Cuts at: 1232 1480 1232
Size: 248 3552
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
SmaI CCC'GGG
Cuts at: 822 822
Size: 3800
SmlI C'TyrA_G
Cuts at: 530 586 955 1870 2362 2624 530
Size: 56 369 915 492 262 1706
Fragments arranged by size:
1706 915 492 369 262 56
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3800
SphI G_CATG'C
Cuts at: 148 853 1353 148
Size: 705 500 2595
Fragments arranged by size:
2595 705 500
SspI AAT'ATT
Cuts at: 384 384
Size: 3800
StyI C'CwwG_G
Cuts at: 114 251 569 934 1288 114
Size: 137 318 365 354 2626
Fragments arranged by size:
2626 365 354 318 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 188 1383 1516 1975 2184 188
Size: 1195 133 459 209 1804
Fragments arranged by size:
1804 1195 459 209 133
TatI w'GTAC_w
Cuts at: 231 471 3315 231
Size: 240 2844 716
Fragments arranged by size:
2844 716 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 3768
Tth111I GACn'n_nGTC
Cuts at: 1597 1597
Size: 3800
XbaI T'CTAG_A
Cuts at: 831 831
Size: 3800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1295 1295
Size: 3800
XhoI C'TCGA_G
Cuts at: 530 530
Size: 3800
XmaI C'CCGG_G
Cuts at: 820 820
Size: 3800
XmnI GAAnn'nnTTC
Cuts at: 158 158
Size: 3800
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI ZraI