Restriction map for
pF1KB6936
(Circular) (Six-base) MAPSORT of: pF1KB6936.seq Check: 2750 from: 1 to: 3800
pF1KB6936 3800 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 838 838
Size: 3800
Acc65I G'GTAC_C
Cuts at: 224 816 224
Size: 592 3208
AclI AA'CG_TT
Cuts at: 3640 3640
Size: 3800
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1625 2057 2804 3072 616
Size: 1009 432 747 268 1344
Fragments arranged by size:
1344 1009 747 432 268
AflIII A'CryG_T
Cuts at: 1152 2256 3233 1152
Size: 1104 977 1719
Fragments arranged by size:
1719 1104 977
AgeI A'CCGG_T
Cuts at: 2188 2188
Size: 3800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 3800
AlwNI CAG_nnn'CTG
Cuts at: 1105 2672 1105
Size: 1567 2233
ApaI G_GGCC'C
Cuts at: 179 653 179
Size: 474 3326
ApaLI G'TGCA_C
Cuts at: 2570 2570
Size: 3800
ApoI r'AATT_y
Cuts at: 804 3153 3409 804
Size: 2349 256 1195
Fragments arranged by size:
2349 1195 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3800
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
AvaI C'yCGr_G
Cuts at: 200 323 562 692 820 200
Size: 123 239 130 128 3180
Fragments arranged by size:
3180 239 130 128 123
BamHI G'GATC_C
Cuts at: 605 825 605
Size: 220 3580
BanI G'GyrC_C
Cuts at: 224 816 1230 1478 1513 224
Size: 592 414 248 35 2511
Fragments arranged by size:
2511 592 414 248 35
BanII G_rGCy'C
Cuts at: 179 448 653 814 1844 179
Size: 269 205 161 1030 2135
Fragments arranged by size:
2135 1030 269 205 161
BbeI G_GCGC'C
Cuts at: 1234 1482 1234
Size: 248 3552
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 441 475 441
Size: 34 3766
BciVI GTATCCnnnnn_n'
Cuts at: 1698 2459 3800 1698
Size: 761 1341 1698
Fragments arranged by size:
1698 1341 761
BclI T'GATC_A
Cuts at: 1320 1320
Size: 3800
BglII A'GATC_T
Cuts at: 584 1315 3352 584
Size: 731 2037 1032
Fragments arranged by size:
2037 1032 731
BlpI GC'TnA_GC
Cuts at: 530 912 530
Size: 382 3418
Bme1580I G_kGCm'C
Cuts at: 179 653 1425 1518 2574 179
Size: 474 772 93 1056 1405
Fragments arranged by size:
1405 1056 772 474 93
BmgBI CAC'GTC
Cuts at: 665 665
Size: 3800
BmrI ACTGGGnnnn_n'
Cuts at: 1012 1093 1428 3572 1012
Size: 81 335 2144 1240
Fragments arranged by size:
2144 1240 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 606 2167 606
Size: 1561 2239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 485 777 432
Size: 53 292 3455
Fragments arranged by size:
3455 292 53
Bpu10I CC'TnA_GC
Cuts at: 517 722 517
Size: 205 3595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1855 2347 2645 976
Size: 879 492 298 2131
Fragments arranged by size:
2131 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3504 3504
Size: 3800
BsaAI yAC'GTr
Cuts at: 302 414 1153 302
Size: 112 739 2949
Fragments arranged by size:
2949 739 112
BsaBI GATnn'nnATC
Cuts at: 6 1314 3351 6
Size: 1308 2037 455
Fragments arranged by size:
2037 1308 455
BsaHI Gr'CG_yC
Cuts at: 355 1231 1479 2181 3677 355
Size: 876 248 702 1496 478
Fragments arranged by size:
1496 876 702 478 248
BsaWI w'CCGG_w
Cuts at: 989 1510 2188 2462 2609 3020 989
Size: 521 678 274 147 411 1769
Fragments arranged by size:
1769 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 180 1225 1983 2560 3056 180
Size: 1045 758 577 496 924
Fragments arranged by size:
1045 924 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 568 357
Size: 211 3589
BsiEI CG_ry'CG
Cuts at: 104 233 1342 1388 2596 104
Size: 129 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 129 46
BsiHKAI G_wGCw'C
Cuts at: 419 482 814 1592 1782 2574 419
Size: 63 332 778 190 792 1645
Fragments arranged by size:
1645 792 778 332 190 63
Bsp1286I G_dGCh'C
Cuts at: 179 419 448 482 653 814 1425 1518 1592
Size: 240 29 34 171 161 611 93 74
Cuts at: 1592 1782 1844 2574 179
Size: 190 62 730 1405
Fragments arranged by size:
1405 730 611 240 190 171 161 93
74 62 34 29
BspEI T'CCGG_A
Cuts at: 3020 3020
Size: 3800
BspHI T'CATG_A
Cuts at: 2976 2976
Size: 3800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 850 1064 1366 1747 850
Size: 214 302 381 2903
Fragments arranged by size:
2903 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2092 2146 3630 3795 50
Size: 2042 54 1484 165 55
Fragments arranged by size:
2042 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 214 293 1712 214
Size: 79 1419 2302
Fragments arranged by size:
2302 1419 79
BsrFI r'CCGG_y
Cuts at: 1798 1979 2188 1798
Size: 181 209 3410
Fragments arranged by size:
3410 209 181
BssHII G'CGCG_C
Cuts at: 631 631
Size: 3800
BssSI C'ACGA_G
Cuts at: 2071 2429 2071
Size: 358 3442
BstAPI GCAn_nnn'nTGC
Cuts at: 1177 1177
Size: 3800
BstBI TT'CG_AA
Cuts at: 2161 2161
Size: 3800
BstYI r'GATC_y
Cuts at: 1 584 605 825 1292 1315 1650 1896 2908
Size: 583 21 220 467 23 335 246 1012
Cuts at: 2908 2994 3006 3352 1
Size: 86 12 346 449
Fragments arranged by size:
1012 583 467 449 346 335 246 220
86 23 21 12
Bsu36I CC'TnA_GG
Cuts at: 676 676
Size: 3800
BtgI C'CryG_G
Cuts at: 236 401 236
Size: 165 3635
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1750 1931 2094 1750
Size: 181 163 3456
Fragments arranged by size:
3456 181 163
BtsI GCAGTG_nn'
Cuts at: 168 193 556 770 1043 168
Size: 25 363 214 273 2925
Fragments arranged by size:
2925 363 273 214 25
DraI TTT'AAA
Cuts at: 799 799
Size: 3800
DraIII CAC_nnn'GTG
Cuts at: 645 645
Size: 3800
DrdI GACnn_nn'nnGTC
Cuts at: 360 1506 2364 360
Size: 1146 858 1796
Fragments arranged by size:
1796 1146 858
EaeI y'GGCC_r
Cuts at: 1385 1559 1950 1977 3322 1385
Size: 174 391 27 1345 1863
Fragments arranged by size:
1863 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1385 1385
Size: 3800
EarI CTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2008 2318 2464 3610 2008
Size: 310 146 1146 2198
Fragments arranged by size:
2198 1146 310 146
EcoICRI GAG'CTC
Cuts at: 812 812
Size: 3800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 485 616 777 1625 2057 2804 3072 432
Size: 53 131 161 848 432 747 268 1160
Fragments arranged by size:
1160 848 747 432 268 161 131 53
EcoNI CCTnn'n_nnAGG
Cuts at: 503 790 503
Size: 287 3513
EcoO109I rG'GnC_Cy
Cuts at: 175 176 566 649 726 939 175
Size: 1 390 83 77 213 3036
Fragments arranged by size:
3036 390 213 83 77 1
EcoRI G'AATT_C
Cuts at: 804 804
Size: 3800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1819 1819
Size: 3800
FspI TGC'GCA
Cuts at: 1581 1581
Size: 3800
HaeII r_GCGC'y
Cuts at: 537 1234 1482 2504 3483 537
Size: 697 248 1022 979 854
Fragments arranged by size:
1022 979 854 697 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1642 1672 1674 1704 1642
Size: 30 2 30 3738
Fragments arranged by size:
3738 30 30 2
HincII GTy'rAC
Cuts at: 839 839
Size: 3800
KasI G'GCGC_C
Cuts at: 1230 1478 1230
Size: 248 3552
KpnI G_GTAC'C
Cuts at: 228 820 228
Size: 592 3208
MluI A'CGCG_T
Cuts at: 3233 3233
Size: 3800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2471 2655 2471
Size: 184 3616
MscI TGG'CCA
Cuts at: 1561 1561
Size: 3800
MslI CAynn'nnrTG
Cuts at: 1789 1916 1789
Size: 127 3673
MspA1I CmG'CkG
Cuts at: 149 335 371 393 911 1225 1585 2598 2843
Size: 186 36 22 518 314 360 1013 245
Cuts at: 2843 149
Size: 1106
Fragments arranged by size:
1106 1013 518 360 314 245 186 36
22
NaeI GCC'GGC
Cuts at: 1981 1981
Size: 3800
NarI GG'CG_CC
Cuts at: 1231 1479 1231
Size: 248 3552
NcoI C'CATG_G
Cuts at: 401 401
Size: 3800
NgoMIV G'CCGG_C
Cuts at: 1979 1979
Size: 3800
NspI r_CATG'y
Cuts at: 853 1353 2260 853
Size: 500 907 2393
Fragments arranged by size:
2393 907 500
PasI CC'CwG_GG
Cuts at: 440 440
Size: 3800
PciI A'CATG_T
Cuts at: 2256 2256
Size: 3800
PflMI CCAn_nnn'nTGG
Cuts at: 645 645
Size: 3800
PfoI T'CCnGG_A
Cuts at: 247 247
Size: 3800
PmeI GTTT'AAAC
Cuts at: 799 799
Size: 3800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2939 2971 2939
Size: 32 3768
PpuMI rG'GwC_Cy
Cuts at: 566 726 566
Size: 160 3640
PshAI GACnn'nnGTC
Cuts at: 758 758
Size: 3800
PspOMI G'GGCC_C
Cuts at: 175 649 175
Size: 474 3326
PstI C_TGCA'G
Cuts at: 163 350 847 1532 163
Size: 187 497 685 2431
Fragments arranged by size:
2431 685 497 187
PvuI CG_AT'CG
Cuts at: 104 233 104
Size: 129 3671
PvuII CAG'CTG
Cuts at: 149 335 371 393 1225 1585 149
Size: 186 36 22 832 360 2364
Fragments arranged by size:
2364 832 360 186 36 22
RsrII CG'GwC_CG
Cuts at: 1995 1995
Size: 3800
SacI G_AGCT'C
Cuts at: 814 814
Size: 3800
SalI G'TCGA_C
Cuts at: 837 837
Size: 3800
SapI GCTCTTCn'nnn_
Cuts at: 1823 2033 1823
Size: 210 3590
SbfI CC_TGCA'GG
Cuts at: 847 847
Size: 3800
ScaI AGT'ACT
Cuts at: 601 3317 601
Size: 2716 1084
SfcI C'TryA_G
Cuts at: 33 159 346 541 843 1528 2521 2712 33
Size: 126 187 195 302 685 993 191 1121
Fragments arranged by size:
1121 993 685 302 195 191 187 126
SfoI GGC'GCC
Cuts at: 1232 1480 1232
Size: 248 3552
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3800
SmaI CCC'GGG
Cuts at: 202 325 564 822 202
Size: 123 239 258 3180
Fragments arranged by size:
3180 258 239 123
SmlI C'TyrA_G
Cuts at: 955 1870 2362 2624 955
Size: 915 492 262 2131
Fragments arranged by size:
2131 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3800
SphI G_CATG'C
Cuts at: 853 1353 853
Size: 500 3300
StyI C'CwwG_G
Cuts at: 401 934 1288 401
Size: 533 354 2913
Fragments arranged by size:
2913 533 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1383 1516 1975 2184 1383
Size: 133 459 209 2999
Fragments arranged by size:
2999 459 209 133
TatI w'GTAC_w
Cuts at: 599 3315 599
Size: 2716 1084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 3768
Tth111I GACn'n_nGTC
Cuts at: 1597 1597
Size: 3800
XbaI T'CTAG_A
Cuts at: 831 831
Size: 3800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 783 1295 783
Size: 512 3288
XmaI C'CCGG_G
Cuts at: 200 323 562 820 200
Size: 123 239 258 3180
Fragments arranged by size:
3180 258 239 123
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmtI BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI