Restriction map for
pF1KB6932
(Circular) (Six-base) MAPSORT of: pF1KB6932.seq Check: 1577 from: 1 to: 3797
pF1KB6932 3797 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 835 835
Size: 3797
Acc65I G'GTAC_C
Cuts at: 813 813
Size: 3797
AclI AA'CG_TT
Cuts at: 777 3637 777
Size: 2860 937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1622 2054 2801 3069 1622
Size: 432 747 268 2350
Fragments arranged by size:
2350 747 432 268
AflIII A'CryG_T
Cuts at: 1149 2253 3230 1149
Size: 1104 977 1716
Fragments arranged by size:
1716 1104 977
AgeI A'CCGG_T
Cuts at: 2185 2185
Size: 3797
AlwNI CAG_nnn'CTG
Cuts at: 766 1102 2669 766
Size: 336 1567 1894
Fragments arranged by size:
1894 1567 336
ApaLI G'TGCA_C
Cuts at: 2567 2567
Size: 3797
ApoI r'AATT_y
Cuts at: 559 801 3150 3406 559
Size: 242 2349 256 950
Fragments arranged by size:
2349 950 256 242
AseI AT'TA_AT
Cuts at: 20 20
Size: 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
AvaI C'yCGr_G
Cuts at: 391 817 391
Size: 426 3371
BamHI G'GATC_C
Cuts at: 822 822
Size: 3797
BanI G'GyrC_C
Cuts at: 492 813 1227 1475 1510 492
Size: 321 414 248 35 2779
Fragments arranged by size:
2779 414 321 248 35
BanII G_rGCy'C
Cuts at: 811 1841 811
Size: 1030 2767
BbeI G_GCGC'C
Cuts at: 1231 1479 1231
Size: 248 3549
BbsI GAAGACnn'nnnn_
Cuts at: 725 725
Size: 3797
BciVI GTATCCnnnnn_n'
Cuts at: 1695 2456 3797 1695
Size: 761 1341 1695
Fragments arranged by size:
1695 1341 761
BclI T'GATC_A
Cuts at: 734 1317 734
Size: 583 3214
BglII A'GATC_T
Cuts at: 727 1312 3349 727
Size: 585 2037 1175
Fragments arranged by size:
2037 1175 585
BlpI GC'TnA_GC
Cuts at: 909 909
Size: 3797
Bme1580I G_kGCm'C
Cuts at: 1422 1515 2571 1422
Size: 93 1056 2648
Fragments arranged by size:
2648 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 597 1009 1090 1425 3569 597
Size: 412 81 335 2144 825
Fragments arranged by size:
2144 825 412 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 3797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1852 2344 2642 973
Size: 879 492 298 2128
Fragments arranged by size:
2128 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3501 3501
Size: 3797
BsaAI yAC'GTr
Cuts at: 1150 1150
Size: 3797
BsaBI GATnn'nnATC
Cuts at: 6 1311 3348 6
Size: 1305 2037 455
Fragments arranged by size:
2037 1305 455
BsaHI Gr'CG_yC
Cuts at: 112 1228 1476 2178 3674 112
Size: 1116 248 702 1496 235
Fragments arranged by size:
1496 1116 702 248 235
BsaWI w'CCGG_w
Cuts at: 179 986 1507 2185 2459 2606 3017 179
Size: 807 521 678 274 147 411 959
Fragments arranged by size:
959 807 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 726 726
Size: 3797
BseYI C'CCAG_C
Cuts at: 1222 1980 2557 3053 1222
Size: 758 577 496 1966
Fragments arranged by size:
1966 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 686 193
Size: 493 3304
BsiEI CG_ry'CG
Cuts at: 104 1339 1385 2593 104
Size: 1235 46 1208 1308
Fragments arranged by size:
1308 1235 1208 46
BsiHKAI G_wGCw'C
Cuts at: 811 1589 1779 2571 811
Size: 778 190 792 2037
Fragments arranged by size:
2037 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 811 1422 1515 1589 1779 1841 2571 811
Size: 611 93 74 190 62 730 2037
Fragments arranged by size:
2037 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 179 3017 179
Size: 2838 959
BspHI T'CATG_A
Cuts at: 2973 2973
Size: 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 847 1061 1363 1744 847
Size: 214 302 381 2900
Fragments arranged by size:
2900 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2089 2143 3627 3792 50
Size: 2039 54 1484 165 55
Fragments arranged by size:
2039 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1709 1709
Size: 3797
BsrFI r'CCGG_y
Cuts at: 1795 1976 2185 1795
Size: 181 209 3407
Fragments arranged by size:
3407 209 181
BssSI C'ACGA_G
Cuts at: 2068 2426 2068
Size: 358 3439
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 1174
Size: 3797
BstBI TT'CG_AA
Cuts at: 279 2158 279
Size: 1879 1918
BstEII G'GTnAC_C
Cuts at: 256 256
Size: 3797
BstXI CCAn_nnnn'nTGG
Cuts at: 341 341
Size: 3797
BstYI r'GATC_y
Cuts at: 1 727 822 1289 1312 1647 1893 2905 2991
Size: 726 95 467 23 335 246 1012 86
Cuts at: 2991 3003 3349 1
Size: 12 346 449
Fragments arranged by size:
1012 726 467 449 346 335 246 95
86 23 12
BtgI C'CryG_G
Cuts at: 107 496 107
Size: 389 3408
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1747 1928 2091 1747
Size: 181 163 3453
Fragments arranged by size:
3453 181 163
BtsI GCAGTG_nn'
Cuts at: 705 1040 705
Size: 335 3462
DraI TTT'AAA
Cuts at: 796 796
Size: 3797
DraIII CAC_nnn'GTG
Cuts at: 264 264
Size: 3797
DrdI GACnn_nn'nnGTC
Cuts at: 1503 2361 1503
Size: 858 2939
EaeI y'GGCC_r
Cuts at: 155 1382 1556 1947 1974 3319 155
Size: 1227 174 391 27 1345 633
Fragments arranged by size:
1345 1227 633 391 174 27
EagI C'GGCC_G
Cuts at: 1382 1382
Size: 3797
EarI CTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2005 2315 2461 3607 2005
Size: 310 146 1146 2195
Fragments arranged by size:
2195 1146 310 146
EcoICRI GAG'CTC
Cuts at: 809 809
Size: 3797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1622 2054 2801 3069 1622
Size: 432 747 268 2350
Fragments arranged by size:
2350 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 297 599 643 936 297
Size: 302 44 293 3158
Fragments arranged by size:
3158 302 293 44
EcoRI G'AATT_C
Cuts at: 801 801
Size: 3797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1816 1816
Size: 3797
FspI TGC'GCA
Cuts at: 1578 1578
Size: 3797
HaeII r_GCGC'y
Cuts at: 1231 1479 2501 3480 1231
Size: 248 1022 979 1548
Fragments arranged by size:
1548 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 712 719 744 751 1639 1669 1671 1701 712
Size: 7 25 7 888 30 2 30 2808
Fragments arranged by size:
2808 888 30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 836 836
Size: 3797
KasI G'GCGC_C
Cuts at: 1227 1475 1227
Size: 248 3549
KpnI G_GTAC'C
Cuts at: 817 817
Size: 3797
MluI A'CGCG_T
Cuts at: 3230 3230
Size: 3797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2652 2468
Size: 184 3613
MscI TGG'CCA
Cuts at: 157 1558 157
Size: 1401 2396
MslI CAynn'nnrTG
Cuts at: 316 322 662 688 1786 1913 316
Size: 6 340 26 1098 127 2200
Fragments arranged by size:
2200 1098 340 127 26 6
MspA1I CmG'CkG
Cuts at: 908 1222 1582 2595 2840 908
Size: 314 360 1013 245 1865
Fragments arranged by size:
1865 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1978 1978
Size: 3797
NarI GG'CG_CC
Cuts at: 1228 1476 1228
Size: 248 3549
NcoI C'CATG_G
Cuts at: 107 496 107
Size: 389 3408
NgoMIV G'CCGG_C
Cuts at: 1976 1976
Size: 3797
NspI r_CATG'y
Cuts at: 315 850 1350 2257 315
Size: 535 500 907 1855
Fragments arranged by size:
1855 907 535 500
PasI CC'CwG_GG
Cuts at: 409 409
Size: 3797
PciI A'CATG_T
Cuts at: 2253 2253
Size: 3797
PmeI GTTT'AAAC
Cuts at: 796 796
Size: 3797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3765
PpuMI rG'GwC_Cy
Cuts at: 297 599 643 297
Size: 302 44 3451
Fragments arranged by size:
3451 302 44
PstI C_TGCA'G
Cuts at: 844 1529 844
Size: 685 3112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3797
PvuII CAG'CTG
Cuts at: 1222 1582 1222
Size: 360 3437
RsrII CG'GwC_CG
Cuts at: 1992 1992
Size: 3797
SacI G_AGCT'C
Cuts at: 811 811
Size: 3797
SalI G'TCGA_C
Cuts at: 834 834
Size: 3797
SapI GCTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
SbfI CC_TGCA'GG
Cuts at: 844 844
Size: 3797
ScaI AGT'ACT
Cuts at: 3314 3314
Size: 3797
SfcI C'TryA_G
Cuts at: 33 359 840 1525 2518 2709 33
Size: 326 481 685 993 191 1121
Fragments arranged by size:
1121 993 685 481 326 191
SfoI GGC'GCC
Cuts at: 1229 1477 1229
Size: 248 3549
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
SmaI CCC'GGG
Cuts at: 819 819
Size: 3797
SmlI C'TyrA_G
Cuts at: 391 952 1867 2359 2621 391
Size: 561 915 492 262 1567
Fragments arranged by size:
1567 915 561 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3797
SphI G_CATG'C
Cuts at: 850 1350 850
Size: 500 3297
StyI C'CwwG_G
Cuts at: 107 496 521 931 1285 107
Size: 389 25 410 354 2619
Fragments arranged by size:
2619 410 389 354 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 1513 1972 2181 1380
Size: 133 459 209 2996
Fragments arranged by size:
2996 459 209 133
TatI w'GTAC_w
Cuts at: 377 3312 377
Size: 2935 862
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 3765
Tth111I GACn'n_nGTC
Cuts at: 652 1594 652
Size: 942 2855
XbaI T'CTAG_A
Cuts at: 828 828
Size: 3797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 587 1292 587
Size: 705 3092
XhoI C'TCGA_G
Cuts at: 391 391
Size: 3797
XmaI C'CCGG_G
Cuts at: 817 817
Size: 3797
XmnI GAAnn'nnTTC
Cuts at: 275 275
Size: 3797
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI