Restriction map for
pF1KB6928
(Circular) (Six-base) MAPSORT of: pF1KB6928.seq Check: 7083 from: 1 to: 3794
pF1KB6928 3794 bp
With 182 enzymes: SgfI *
April 6, 2009 09:17 ..
AccI GT'mk_AC
Cuts at: 322 832 322
Size: 510 3284
Acc65I G'GTAC_C
Cuts at: 810 810
Size: 3794
AclI AA'CG_TT
Cuts at: 3634 3634
Size: 3794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1619 2051 2798 3066 778
Size: 841 432 747 268 1506
Fragments arranged by size:
1506 841 747 432 268
AflIII A'CryG_T
Cuts at: 1146 2250 3227 1146
Size: 1104 977 1713
Fragments arranged by size:
1713 1104 977
AgeI A'CCGG_T
Cuts at: 2182 2182
Size: 3794
AlwNI CAG_nnn'CTG
Cuts at: 1099 2666 1099
Size: 1567 2227
ApaLI G'TGCA_C
Cuts at: 2564 2564
Size: 3794
ApoI r'AATT_y
Cuts at: 307 798 3147 3403 307
Size: 491 2349 256 698
Fragments arranged by size:
2349 698 491 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3794
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
AvaI C'yCGr_G
Cuts at: 814 814
Size: 3794
BamHI G'GATC_C
Cuts at: 819 819
Size: 3794
BanI G'GyrC_C
Cuts at: 810 1224 1472 1507 810
Size: 414 248 35 3097
Fragments arranged by size:
3097 414 248 35
BanII G_rGCy'C
Cuts at: 808 1838 808
Size: 1030 2764
BbeI G_GCGC'C
Cuts at: 1228 1476 1228
Size: 248 3546
BbsI GAAGACnn'nnnn_
Cuts at: 398 398
Size: 3794
BciVI GTATCCnnnnn_n'
Cuts at: 1692 2453 3794 1692
Size: 761 1341 1692
Fragments arranged by size:
1692 1341 761
BclI T'GATC_A
Cuts at: 1314 1314
Size: 3794
BglI GCCn_nnn'nGGC
Cuts at: 520 520
Size: 3794
BglII A'GATC_T
Cuts at: 626 1309 3346 626
Size: 683 2037 1074
Fragments arranged by size:
2037 1074 683
BlpI GC'TnA_GC
Cuts at: 906 906
Size: 3794
Bme1580I G_kGCm'C
Cuts at: 1419 1512 2568 1419
Size: 93 1056 2645
Fragments arranged by size:
2645 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1006 1087 1422 3566 1006
Size: 81 335 2144 1234
Fragments arranged by size:
2144 1234 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 3794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 970 1849 2341 2639 733
Size: 237 879 492 298 1888
Fragments arranged by size:
1888 879 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 233 3498 233
Size: 3265 529
BsaAI yAC'GTr
Cuts at: 499 1147 499
Size: 648 3146
BsaBI GATnn'nnATC
Cuts at: 6 1308 3345 6
Size: 1302 2037 455
Fragments arranged by size:
2037 1302 455
BsaHI Gr'CG_yC
Cuts at: 1225 1473 2175 3671 1225
Size: 248 702 1496 1348
Fragments arranged by size:
1496 1348 702 248
BsaWI w'CCGG_w
Cuts at: 983 1504 2182 2456 2603 3014 983
Size: 521 678 274 147 411 1763
Fragments arranged by size:
1763 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 279 565 279
Size: 286 3508
BseYI C'CCAG_C
Cuts at: 1219 1977 2554 3050 1219
Size: 758 577 496 1963
Fragments arranged by size:
1963 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1336 1382 2590 104
Size: 1232 46 1208 1308
Fragments arranged by size:
1308 1232 1208 46
BsiHKAI G_wGCw'C
Cuts at: 808 1586 1776 2568 808
Size: 778 190 792 2034
Fragments arranged by size:
2034 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 808 1419 1512 1586 1776 1838 2568 808
Size: 611 93 74 190 62 730 2034
Fragments arranged by size:
2034 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3014 3014
Size: 3794
BspHI T'CATG_A
Cuts at: 2970 2970
Size: 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 173 710 844 1058 1360 1741 173
Size: 537 134 214 302 381 2226
Fragments arranged by size:
2226 537 381 302 214 134
BsrBI CCG'CTC
Cuts at: 50 2086 2140 3624 3789 50
Size: 2036 54 1484 165 55
Fragments arranged by size:
2036 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1706 1706
Size: 3794
BsrFI r'CCGG_y
Cuts at: 1792 1973 2182 1792
Size: 181 209 3404
Fragments arranged by size:
3404 209 181
BssSI C'ACGA_G
Cuts at: 2065 2423 2065
Size: 358 3436
BstAPI GCAn_nnn'nTGC
Cuts at: 1171 1171
Size: 3794
BstBI TT'CG_AA
Cuts at: 2155 2155
Size: 3794
BstEII G'GTnAC_C
Cuts at: 228 228
Size: 3794
BstYI r'GATC_y
Cuts at: 1 626 819 1286 1309 1644 1890 2902 2988
Size: 625 193 467 23 335 246 1012 86
Cuts at: 2988 3000 3346 1
Size: 12 346 449
Fragments arranged by size:
1012 625 467 449 346 335 246 193
86 23 12
BstZ17I GTA'TAC
Cuts at: 323 323
Size: 3794
BtgI C'CryG_G
Cuts at: 127 327 127
Size: 200 3594
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1744 1925 2088 1744
Size: 181 163 3450
Fragments arranged by size:
3450 181 163
BtsI GCAGTG_nn'
Cuts at: 708 1037 708
Size: 329 3465
DraI TTT'AAA
Cuts at: 793 793
Size: 3794
DrdI GACnn_nn'nnGTC
Cuts at: 256 1500 2358 256
Size: 1244 858 1692
Fragments arranged by size:
1692 1244 858
EaeI y'GGCC_r
Cuts at: 330 1379 1553 1944 1971 3316 330
Size: 1049 174 391 27 1345 808
Fragments arranged by size:
1345 1049 808 391 174 27
EagI C'GGCC_G
Cuts at: 1379 1379
Size: 3794
EarI CTCTTCn'nnn_
Cuts at: 668 1817 2027 668
Size: 1149 210 2435
Fragments arranged by size:
2435 1149 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2002 2312 2458 3604 2002
Size: 310 146 1146 2192
Fragments arranged by size:
2192 1146 310 146
EcoICRI GAG'CTC
Cuts at: 806 806
Size: 3794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 1619 2051 2798 3066 778
Size: 841 432 747 268 1506
Fragments arranged by size:
1506 841 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 137 933 137
Size: 796 2998
EcoRI G'AATT_C
Cuts at: 307 798 307
Size: 491 3303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1813 1813
Size: 3794
FspI TGC'GCA
Cuts at: 1575 1575
Size: 3794
HaeII r_GCGC'y
Cuts at: 1228 1476 2498 3477 1228
Size: 248 1022 979 1545
Fragments arranged by size:
1545 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 661 693 1636 1666 1668 1698 661
Size: 32 943 30 2 30 2757
Fragments arranged by size:
2757 943 32 30 30 2
HincII GTy'rAC
Cuts at: 833 833
Size: 3794
KasI G'GCGC_C
Cuts at: 1224 1472 1224
Size: 248 3546
KpnI G_GTAC'C
Cuts at: 814 814
Size: 3794
MluI A'CGCG_T
Cuts at: 3227 3227
Size: 3794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2649 2465
Size: 184 3610
MscI TGG'CCA
Cuts at: 1555 1555
Size: 3794
MslI CAynn'nnrTG
Cuts at: 1783 1910 1783
Size: 127 3667
MspA1I CmG'CkG
Cuts at: 362 905 1219 1579 2592 2837 362
Size: 543 314 360 1013 245 1319
Fragments arranged by size:
1319 1013 543 360 314 245
NaeI GCC'GGC
Cuts at: 1975 1975
Size: 3794
NarI GG'CG_CC
Cuts at: 1225 1473 1225
Size: 248 3546
NcoI C'CATG_G
Cuts at: 127 327 127
Size: 200 3594
NgoMIV G'CCGG_C
Cuts at: 1973 1973
Size: 3794
NspI r_CATG'y
Cuts at: 847 1347 2254 847
Size: 500 907 2387
Fragments arranged by size:
2387 907 500
PciI A'CATG_T
Cuts at: 2250 2250
Size: 3794
PflMI CCAn_nnn'nTGG
Cuts at: 183 183
Size: 3794
PfoI T'CCnGG_A
Cuts at: 460 460
Size: 3794
PmeI GTTT'AAAC
Cuts at: 793 793
Size: 3794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2933 2965 2933
Size: 32 3762
PpuMI rG'GwC_Cy
Cuts at: 137 137
Size: 3794
PsiI TTA'TAA
Cuts at: 698 698
Size: 3794
PstI C_TGCA'G
Cuts at: 662 841 1526 662
Size: 179 685 2930
Fragments arranged by size:
2930 685 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3794
PvuII CAG'CTG
Cuts at: 362 1219 1579 362
Size: 857 360 2577
Fragments arranged by size:
2577 857 360
RsrII CG'GwC_CG
Cuts at: 1989 1989
Size: 3794
SacI G_AGCT'C
Cuts at: 808 808
Size: 3794
SalI G'TCGA_C
Cuts at: 831 831
Size: 3794
SanDI GG'GwC_CC
Cuts at: 137 137
Size: 3794
SapI GCTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
SbfI CC_TGCA'GG
Cuts at: 841 841
Size: 3794
ScaI AGT'ACT
Cuts at: 3311 3311
Size: 3794
SfcI C'TryA_G
Cuts at: 33 539 658 837 1522 2515 2706 33
Size: 506 119 179 685 993 191 1121
Fragments arranged by size:
1121 993 685 506 191 179 119
SfoI GGC'GCC
Cuts at: 1226 1474 1226
Size: 248 3546
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
SmaI CCC'GGG
Cuts at: 816 816
Size: 3794
SmlI C'TyrA_G
Cuts at: 748 949 1864 2356 2618 748
Size: 201 915 492 262 1924
Fragments arranged by size:
1924 915 492 262 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3794
SphI G_CATG'C
Cuts at: 847 1347 847
Size: 500 3294
StyI C'CwwG_G
Cuts at: 127 327 347 928 1282 127
Size: 200 20 581 354 2639
Fragments arranged by size:
2639 581 354 200 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1377 1510 1969 2178 1377
Size: 133 459 209 2993
Fragments arranged by size:
2993 459 209 133
TatI w'GTAC_w
Cuts at: 3309 3309
Size: 3794
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 358 390 3311 3343 358
Size: 32 2921 32 809
Fragments arranged by size:
2921 809 32 32
Tth111I GACn'n_nGTC
Cuts at: 1591 1591
Size: 3794
XbaI T'CTAG_A
Cuts at: 570 825 570
Size: 255 3539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1289 1289
Size: 3794
XmaI C'CCGG_G
Cuts at: 814 814
Size: 3794
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstXI Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI