Restriction map for
pF1KB6904
(Circular) (Six-base) MAPSORT of: pF1KB6904.seq Check: 5094 from: 1 to: 3782
pF1KB6904 3782 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 820 820
Size: 3782
Acc65I G'GTAC_C
Cuts at: 798 798
Size: 3782
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 1607 2039 2786 3054 374
Size: 1233 432 747 268 1102
Fragments arranged by size:
1233 1102 747 432 268
AflIII A'CryG_T
Cuts at: 1134 2238 3215 1134
Size: 1104 977 1701
Fragments arranged by size:
1701 1104 977
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 235 235
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 164 586 1087 2654 164
Size: 422 501 1567 1292
Fragments arranged by size:
1567 1292 501 422
ApaLI G'TGCA_C
Cuts at: 2552 2552
Size: 3782
ApoI r'AATT_y
Cuts at: 786 3135 3391 786
Size: 2349 256 1177
Fragments arranged by size:
2349 1177 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3782
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 802 802
Size: 3782
BamHI G'GATC_C
Cuts at: 602 807 602
Size: 205 3577
BanI G'GyrC_C
Cuts at: 421 798 1212 1460 1495 421
Size: 377 414 248 35 2708
Fragments arranged by size:
2708 414 377 248 35
BanII G_rGCy'C
Cuts at: 506 796 1826 506
Size: 290 1030 2462
Fragments arranged by size:
2462 1030 290
BbeI G_GCGC'C
Cuts at: 1216 1464 1216
Size: 248 3534
BbsI GAAGACnn'nnnn_
Cuts at: 198 198
Size: 3782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 602 636 602
Size: 34 3748
BciVI GTATCCnnnnn_n'
Cuts at: 1680 2441 3782 1680
Size: 761 1341 1680
Fragments arranged by size:
1680 1341 761
BclI T'GATC_A
Cuts at: 1302 1302
Size: 3782
BfrBI ATG'CAT
Cuts at: 289 289
Size: 3782
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 140 894 140
Size: 754 3028
Bme1580I G_kGCm'C
Cuts at: 424 580 1407 1500 2556 424
Size: 156 827 93 1056 1650
Fragments arranged by size:
1650 1056 827 156 93
BmrI ACTGGGnnnn_n'
Cuts at: 994 1075 1410 3554 994
Size: 81 335 2144 1222
Fragments arranged by size:
2144 1222 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 3782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 411 278
Size: 133 3649
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1837 2329 2627 958
Size: 879 492 298 2113
Fragments arranged by size:
2113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3486 3486
Size: 3782
BsaAI yAC'GTr
Cuts at: 225 1135 225
Size: 910 2872
BsaBI GATnn'nnATC
Cuts at: 6 1296 3333 6
Size: 1290 2037 455
Fragments arranged by size:
2037 1290 455
BsaHI Gr'CG_yC
Cuts at: 1213 1461 2163 3659 1213
Size: 248 702 1496 1336
Fragments arranged by size:
1496 1336 702 248
BsaWI w'CCGG_w
Cuts at: 971 1492 2170 2444 2591 3002 971
Size: 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 493 523 493
Size: 30 3752
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 637 586
Size: 51 3731
BseYI C'CCAG_C
Cuts at: 230 1207 1965 2542 3038 230
Size: 977 758 577 496 974
Fragments arranged by size:
977 974 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 399 156
Size: 243 3539
BsiEI CG_ry'CG
Cuts at: 104 1324 1370 2578 104
Size: 1220 46 1208 1308
Fragments arranged by size:
1308 1220 1208 46
BsiHKAI G_wGCw'C
Cuts at: 506 796 1574 1764 2556 506
Size: 290 778 190 792 1732
Fragments arranged by size:
1732 792 778 290 190
Bsp1286I G_dGCh'C
Cuts at: 424 506 580 796 1407 1500 1574 1764 1826
Size: 82 74 216 611 93 74 190 62
Cuts at: 1826 2556 424
Size: 730 1650
Fragments arranged by size:
1650 730 611 216 190 93 82 74
74 62
BspEI T'CCGG_A
Cuts at: 3002 3002
Size: 3782
BspHI T'CATG_A
Cuts at: 2958 2958
Size: 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1046 1348 1729 832
Size: 214 302 381 2885
Fragments arranged by size:
2885 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2074 2128 3612 3777 50
Size: 2024 54 1484 165 55
Fragments arranged by size:
2024 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 224 1694 224
Size: 1470 2312
BsrFI r'CCGG_y
Cuts at: 1780 1961 2170 1780
Size: 181 209 3392
Fragments arranged by size:
3392 209 181
BssSI C'ACGA_G
Cuts at: 2053 2411 2053
Size: 358 3424
BstAPI GCAn_nnn'nTGC
Cuts at: 1159 1159
Size: 3782
BstBI TT'CG_AA
Cuts at: 2143 2143
Size: 3782
BstYI r'GATC_y
Cuts at: 1 602 807 1274 1297 1632 1878 2890 2976
Size: 601 205 467 23 335 246 1012 86
Cuts at: 2976 2988 3334 1
Size: 12 346 449
Fragments arranged by size:
1012 601 467 449 346 335 246 205
86 23 12
BtgI C'CryG_G
Cuts at: 107 470 107
Size: 363 3419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 249 1732 1913 2076 249
Size: 1483 181 163 1955
Fragments arranged by size:
1955 1483 181 163
BtsI GCAGTG_nn'
Cuts at: 1025 1025
Size: 3782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 206 241 206
Size: 35 3747
DraI TTT'AAA
Cuts at: 781 781
Size: 3782
DrdI GACnn_nn'nnGTC
Cuts at: 1488 2346 1488
Size: 858 2924
EaeI y'GGCC_r
Cuts at: 248 1367 1541 1932 1959 3304 248
Size: 1119 174 391 27 1345 726
Fragments arranged by size:
1345 1119 726 391 174 27
EagI C'GGCC_G
Cuts at: 1367 1367
Size: 3782
EarI CTCTTCn'nnn_
Cuts at: 199 1805 2015 199
Size: 1606 210 1966
Fragments arranged by size:
1966 1606 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 2300 2446 3592 1990
Size: 310 146 1146 2180
Fragments arranged by size:
2180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 504 794 504
Size: 290 3492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 374 411 1607 2039 2786 3054 278
Size: 96 37 1196 432 747 268 1006
Fragments arranged by size:
1196 1006 747 432 268 96 37
EcoO109I rG'GnC_Cy
Cuts at: 161 921 161
Size: 760 3022
EcoRI G'AATT_C
Cuts at: 786 786
Size: 3782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1801 1801
Size: 3782
FspI TGC'GCA
Cuts at: 1563 1563
Size: 3782
HaeII r_GCGC'y
Cuts at: 1216 1464 2486 3465 1216
Size: 248 1022 979 1533
Fragments arranged by size:
1533 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 473 505 1624 1654 1656 1686 274
Size: 32 167 32 1119 30 2 30 2370
Fragments arranged by size:
2370 1119 167 32 32 30 30 2
HincII GTy'rAC
Cuts at: 392 821 392
Size: 429 3353
KasI G'GCGC_C
Cuts at: 1212 1460 1212
Size: 248 3534
KpnI G_GTAC'C
Cuts at: 802 802
Size: 3782
MluI A'CGCG_T
Cuts at: 3215 3215
Size: 3782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2453 2637 2453
Size: 184 3598
MscI TGG'CCA
Cuts at: 250 1543 250
Size: 1293 2489
MslI CAynn'nnrTG
Cuts at: 1771 1898 1771
Size: 127 3655
MspA1I CmG'CkG
Cuts at: 129 144 655 893 1207 1567 2580 2825 129
Size: 15 511 238 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 511 360 314 245 238 15
NaeI GCC'GGC
Cuts at: 1963 1963
Size: 3782
NarI GG'CG_CC
Cuts at: 1213 1461 1213
Size: 248 3534
NcoI C'CATG_G
Cuts at: 107 470 107
Size: 363 3419
NgoMIV G'CCGG_C
Cuts at: 1961 1961
Size: 3782
NsiI A_TGCA'T
Cuts at: 291 291
Size: 3782
NspI r_CATG'y
Cuts at: 835 1335 2242 835
Size: 500 907 2375
Fragments arranged by size:
2375 907 500
PciI A'CATG_T
Cuts at: 2238 2238
Size: 3782
PflMI CCAn_nnn'nTGG
Cuts at: 164 164
Size: 3782
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PmlI CAC'GTG
Cuts at: 225 225
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PpuMI rG'GwC_Cy
Cuts at: 161 161
Size: 3782
PshAI GACnn'nnGTC
Cuts at: 355 355
Size: 3782
PstI C_TGCA'G
Cuts at: 829 1514 829
Size: 685 3097
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3782
PvuII CAG'CTG
Cuts at: 144 655 1207 1567 144
Size: 511 552 360 2359
Fragments arranged by size:
2359 552 511 360
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 506 796 506
Size: 290 3492
SalI G'TCGA_C
Cuts at: 819 819
Size: 3782
SapI GCTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
SbfI CC_TGCA'GG
Cuts at: 829 829
Size: 3782
ScaI AGT'ACT
Cuts at: 3299 3299
Size: 3782
SfcI C'TryA_G
Cuts at: 33 825 1510 2503 2694 33
Size: 792 685 993 191 1121
Fragments arranged by size:
1121 993 792 685 191
SfoI GGC'GCC
Cuts at: 1214 1462 1214
Size: 248 3534
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 804 804
Size: 3782
SmlI C'TyrA_G
Cuts at: 937 1852 2344 2606 937
Size: 915 492 262 2113
Fragments arranged by size:
2113 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3782
SphI G_CATG'C
Cuts at: 835 1335 835
Size: 500 3282
StyI C'CwwG_G
Cuts at: 107 117 456 470 916 1270 107
Size: 10 339 14 446 354 2619
Fragments arranged by size:
2619 446 354 339 14 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1365 1498 1957 2166 1365
Size: 133 459 209 2981
Fragments arranged by size:
2981 459 209 133
TatI w'GTAC_w
Cuts at: 3297 3297
Size: 3782
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 425 457 3299 3331 425
Size: 32 2842 32 876
Fragments arranged by size:
2842 876 32 32
Tth111I GACn'n_nGTC
Cuts at: 1579 1579
Size: 3782
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1277 1277
Size: 3782
XmaI C'CCGG_G
Cuts at: 802 802
Size: 3782
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PfoI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI