Restriction map for
pF1KB6892
(Circular) (Six-base) MAPSORT of: pF1KB6892.seq Check: 9812 from: 1 to: 3719
pF1KB6892 3719 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 163 163
Size: 3719
AccI GT'mk_AC
Cuts at: 757 757
Size: 3719
Acc65I G'GTAC_C
Cuts at: 735 735
Size: 3719
AclI AA'CG_TT
Cuts at: 3559 3559
Size: 3719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1976 2723 2991 1544
Size: 432 747 268 2272
Fragments arranged by size:
2272 747 432 268
AfeI AGC'GCT
Cuts at: 373 373
Size: 3719
AflIII A'CryG_T
Cuts at: 1071 2175 3152 1071
Size: 1104 977 1638
Fragments arranged by size:
1638 1104 977
AgeI A'CCGG_T
Cuts at: 2107 2107
Size: 3719
AlwNI CAG_nnn'CTG
Cuts at: 651 661 1024 2591 651
Size: 10 363 1567 1779
Fragments arranged by size:
1779 1567 363 10
ApaI G_GGCC'C
Cuts at: 589 589
Size: 3719
ApaLI G'TGCA_C
Cuts at: 2489 2489
Size: 3719
ApoI r'AATT_y
Cuts at: 723 3072 3328 723
Size: 2349 256 1114
Fragments arranged by size:
2349 1114 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
AvaI C'yCGr_G
Cuts at: 739 739
Size: 3719
BamHI G'GATC_C
Cuts at: 744 744
Size: 3719
BanI G'GyrC_C
Cuts at: 735 1149 1397 1432 735
Size: 414 248 35 3022
Fragments arranged by size:
3022 414 248 35
BanII G_rGCy'C
Cuts at: 554 589 733 1763 554
Size: 35 144 1030 2510
Fragments arranged by size:
2510 1030 144 35
BbeI G_GCGC'C
Cuts at: 1153 1401 1153
Size: 248 3471
BbsI GAAGACnn'nnnn_
Cuts at: 189 189
Size: 3719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 582 616 582
Size: 34 3685
BciVI GTATCCnnnnn_n'
Cuts at: 156 1617 2378 3719 156
Size: 1461 761 1341 156
Fragments arranged by size:
1461 1341 761 156
BclI T'GATC_A
Cuts at: 1239 1239
Size: 3719
BglI GCCn_nnn'nGGC
Cuts at: 436 436
Size: 3719
BglII A'GATC_T
Cuts at: 1234 3271 1234
Size: 2037 1682
BlpI GC'TnA_GC
Cuts at: 831 831
Size: 3719
Bme1580I G_kGCm'C
Cuts at: 589 1344 1437 2493 589
Size: 755 93 1056 1815
Fragments arranged by size:
1815 1056 755 93
BmrI ACTGGGnnnn_n'
Cuts at: 167 273 931 1012 1347 3491 167
Size: 106 658 81 335 2144 395
Fragments arranged by size:
2144 658 395 335 106 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 3719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 292
Size: 3719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1774 2266 2564 895
Size: 879 492 298 2050
Fragments arranged by size:
2050 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3423 3423
Size: 3719
BsaAI yAC'GTr
Cuts at: 1072 1072
Size: 3719
BsaBI GATnn'nnATC
Cuts at: 6 1233 3270 6
Size: 1227 2037 455
Fragments arranged by size:
2037 1227 455
BsaHI Gr'CG_yC
Cuts at: 595 1150 1398 2100 3596 595
Size: 555 248 702 1496 718
Fragments arranged by size:
1496 718 702 555 248
BsaWI w'CCGG_w
Cuts at: 908 1429 2107 2381 2528 2939 908
Size: 521 678 274 147 411 1688
Fragments arranged by size:
1688 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 193
Size: 3719
BseYI C'CCAG_C
Cuts at: 1144 1902 2479 2975 1144
Size: 758 577 496 1888
Fragments arranged by size:
1888 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1261 1307 2515 104
Size: 1157 46 1208 1308
Fragments arranged by size:
1308 1208 1157 46
BsiHKAI G_wGCw'C
Cuts at: 733 1511 1701 2493 733
Size: 778 190 792 1959
Fragments arranged by size:
1959 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 554 589 733 1344 1437 1511 1701 1763 2493
Size: 35 144 611 93 74 190 62 730
Cuts at: 2493 554
Size: 1780
Fragments arranged by size:
1780 730 611 190 144 93 74 62
35
BspEI T'CCGG_A
Cuts at: 2939 2939
Size: 3719
BspHI T'CATG_A
Cuts at: 2895 2895
Size: 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 163 769 983 1285 1666 163
Size: 606 214 302 381 2216
Fragments arranged by size:
2216 606 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2011 2065 3549 3714 50
Size: 1961 54 1484 165 55
Fragments arranged by size:
1961 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1631
Size: 3719
BsrFI r'CCGG_y
Cuts at: 478 1717 1898 2107 478
Size: 1239 181 209 2090
Fragments arranged by size:
2090 1239 209 181
BssSI C'ACGA_G
Cuts at: 1990 2348 1990
Size: 358 3361
BstAPI GCAn_nnn'nTGC
Cuts at: 1096 1096
Size: 3719
BstBI TT'CG_AA
Cuts at: 2080 2080
Size: 3719
BstXI CCAn_nnnn'nTGG
Cuts at: 159 159
Size: 3719
BstYI r'GATC_y
Cuts at: 1 744 1211 1234 1569 1815 2827 2913 2925
Size: 743 467 23 335 246 1012 86 12
Cuts at: 2925 3271 1
Size: 346 449
Fragments arranged by size:
1012 743 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 700 700
Size: 3719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1669 1850 2013 1669
Size: 181 163 3375
Fragments arranged by size:
3375 181 163
BtsI GCAGTG_nn'
Cuts at: 962 962
Size: 3719
DraI TTT'AAA
Cuts at: 718 718
Size: 3719
DraIII CAC_nnn'GTG
Cuts at: 175 175
Size: 3719
DrdI GACnn_nn'nnGTC
Cuts at: 193 1425 2283 193
Size: 1232 858 1629
Fragments arranged by size:
1629 1232 858
EaeI y'GGCC_r
Cuts at: 428 476 1304 1478 1869 1896 3241 428
Size: 48 828 174 391 27 1345 906
Fragments arranged by size:
1345 906 828 391 174 48 27
EagI C'GGCC_G
Cuts at: 1304 1304
Size: 3719
EarI CTCTTCn'nnn_
Cuts at: 308 326 1742 1952 308
Size: 18 1416 210 2075
Fragments arranged by size:
2075 1416 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1927 2237 2383 3529 1927
Size: 310 146 1146 2117
Fragments arranged by size:
2117 1146 310 146
EcoICRI GAG'CTC
Cuts at: 731 731
Size: 3719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1544 1976 2723 2991 292
Size: 1252 432 747 268 1020
Fragments arranged by size:
1252 1020 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 205 267 858 205
Size: 62 591 3066
Fragments arranged by size:
3066 591 62
EcoRI G'AATT_C
Cuts at: 723 723
Size: 3719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1738 1738
Size: 3719
FseI GG_CCGG'CC
Cuts at: 482 482
Size: 3719
FspI TGC'GCA
Cuts at: 1500 1500
Size: 3719
HaeII r_GCGC'y
Cuts at: 375 652 1153 1401 2423 3402 375
Size: 277 501 248 1022 979 692
Fragments arranged by size:
1022 979 692 501 277 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1561 1591 1593 1623 1561
Size: 30 2 30 3657
Fragments arranged by size:
3657 30 30 2
HincII GTy'rAC
Cuts at: 758 758
Size: 3719
KasI G'GCGC_C
Cuts at: 1149 1397 1149
Size: 248 3471
KpnI G_GTAC'C
Cuts at: 739 739
Size: 3719
MluI A'CGCG_T
Cuts at: 3152 3152
Size: 3719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2574 2390
Size: 184 3535
MscI TGG'CCA
Cuts at: 430 1480 430
Size: 1050 2669
MslI CAynn'nnrTG
Cuts at: 1708 1835 1708
Size: 127 3592
MspA1I CmG'CkG
Cuts at: 830 1144 1504 2517 2762 830
Size: 314 360 1013 245 1787
Fragments arranged by size:
1787 1013 360 314 245
NaeI GCC'GGC
Cuts at: 480 1900 480
Size: 1420 2299
NarI GG'CG_CC
Cuts at: 1150 1398 1150
Size: 248 3471
NgoMIV G'CCGG_C
Cuts at: 478 1898 478
Size: 1420 2299
NspI r_CATG'y
Cuts at: 772 1272 2179 772
Size: 500 907 2312
Fragments arranged by size:
2312 907 500
PciI A'CATG_T
Cuts at: 2175 2175
Size: 3719
PflMI CCAn_nnn'nTGG
Cuts at: 263 263
Size: 3719
PmeI GTTT'AAAC
Cuts at: 718 718
Size: 3719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 3687
PpuMI rG'GwC_Cy
Cuts at: 205 267 205
Size: 62 3657
PspOMI G'GGCC_C
Cuts at: 585 585
Size: 3719
PstI C_TGCA'G
Cuts at: 766 1451 766
Size: 685 3034
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3719
PvuII CAG'CTG
Cuts at: 1144 1504 1144
Size: 360 3359
RsrII CG'GwC_CG
Cuts at: 1914 1914
Size: 3719
SacI G_AGCT'C
Cuts at: 733 733
Size: 3719
SalI G'TCGA_C
Cuts at: 756 756
Size: 3719
SanDI GG'GwC_CC
Cuts at: 205 205
Size: 3719
SapI GCTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
SbfI CC_TGCA'GG
Cuts at: 766 766
Size: 3719
ScaI AGT'ACT
Cuts at: 3236 3236
Size: 3719
SfcI C'TryA_G
Cuts at: 33 762 1447 2440 2631 33
Size: 729 685 993 191 1121
Fragments arranged by size:
1121 993 729 685 191
SfoI GGC'GCC
Cuts at: 1151 1399 1151
Size: 248 3471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
SmaI CCC'GGG
Cuts at: 741 741
Size: 3719
SmlI C'TyrA_G
Cuts at: 874 1789 2281 2543 874
Size: 915 492 262 2050
Fragments arranged by size:
2050 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3719
SphI G_CATG'C
Cuts at: 772 1272 772
Size: 500 3219
StuI AGG'CCT
Cuts at: 629 708 629
Size: 79 3640
StyI C'CwwG_G
Cuts at: 853 1207 853
Size: 354 3365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 186 1302 1435 1894 2103 186
Size: 1116 133 459 209 1802
Fragments arranged by size:
1802 1116 459 209 133
TatI w'GTAC_w
Cuts at: 3234 3234
Size: 3719
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 289 304 321 336 3236 3268 289
Size: 15 17 15 2900 32 740
Fragments arranged by size:
2900 740 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1516 1516
Size: 3719
XbaI T'CTAG_A
Cuts at: 750 750
Size: 3719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 438 1214 438
Size: 776 2943
XmaI C'CCGG_G
Cuts at: 739 739
Size: 3719
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I BtgI
ClaI CspCI EcoNI EcoRV FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI