Restriction map for
pF1KB6887
(Circular) (Six-base) MAPSORT of: pF1KB6887.seq Check: 2086 from: 1 to: 3662
pF1KB6887 3662 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 700 700
Size: 3662
Acc65I G'GTAC_C
Cuts at: 678 678
Size: 3662
AclI AA'CG_TT
Cuts at: 3502 3502
Size: 3662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1487 1919 2666 2934 1487
Size: 432 747 268 2215
Fragments arranged by size:
2215 747 432 268
AflIII A'CryG_T
Cuts at: 1014 2118 3095 1014
Size: 1104 977 1581
Fragments arranged by size:
1581 1104 977
AgeI A'CCGG_T
Cuts at: 2050 2050
Size: 3662
AlwNI CAG_nnn'CTG
Cuts at: 382 644 967 2534 382
Size: 262 323 1567 1510
Fragments arranged by size:
1567 1510 323 262
ApaLI G'TGCA_C
Cuts at: 417 2432 417
Size: 2015 1647
ApoI r'AATT_y
Cuts at: 666 3015 3271 666
Size: 2349 256 1057
Fragments arranged by size:
2349 1057 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
AvaI C'yCGr_G
Cuts at: 682 682
Size: 3662
BamHI G'GATC_C
Cuts at: 687 687
Size: 3662
BanI G'GyrC_C
Cuts at: 678 1092 1340 1375 678
Size: 414 248 35 2965
Fragments arranged by size:
2965 414 248 35
BanII G_rGCy'C
Cuts at: 374 548 676 1706 374
Size: 174 128 1030 2330
Fragments arranged by size:
2330 1030 174 128
BbeI G_GCGC'C
Cuts at: 1096 1344 1096
Size: 248 3414
BciVI GTATCCnnnnn_n'
Cuts at: 1560 2321 3662 1560
Size: 761 1341 1560
Fragments arranged by size:
1560 1341 761
BclI T'GATC_A
Cuts at: 1182 1182
Size: 3662
BglII A'GATC_T
Cuts at: 512 1177 3214 512
Size: 665 2037 960
Fragments arranged by size:
2037 960 665
BlpI GC'TnA_GC
Cuts at: 774 774
Size: 3662
Bme1580I G_kGCm'C
Cuts at: 421 537 1287 1380 2436 421
Size: 116 750 93 1056 1647
Fragments arranged by size:
1647 1056 750 116 93
BmgBI CAC'GTC
Cuts at: 204 204
Size: 3662
BmrI ACTGGGnnnn_n'
Cuts at: 874 955 1290 3434 874
Size: 81 335 2144 1102
Fragments arranged by size:
2144 1102 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 577 2029 577
Size: 1452 2210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 388
Size: 3662
Bpu10I CC'TnA_GC
Cuts at: 158 158
Size: 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1717 2209 2507 838
Size: 879 492 298 1993
Fragments arranged by size:
1993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3366 3366
Size: 3662
BsaAI yAC'GTr
Cuts at: 1015 1015
Size: 3662
BsaBI GATnn'nnATC
Cuts at: 6 1176 3213 6
Size: 1170 2037 455
Fragments arranged by size:
2037 1170 455
BsaHI Gr'CG_yC
Cuts at: 1093 1341 2043 3539 1093
Size: 248 702 1496 1216
Fragments arranged by size:
1496 1216 702 248
BsaWI w'CCGG_w
Cuts at: 851 1372 2050 2324 2471 2882 851
Size: 521 678 274 147 411 1631
Fragments arranged by size:
1631 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 562 592 562
Size: 30 3632
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 260 538 556 260
Size: 278 18 3366
Fragments arranged by size:
3366 278 18
BseYI C'CCAG_C
Cuts at: 305 333 1087 1845 2422 2918 305
Size: 28 754 758 577 496 1049
Fragments arranged by size:
1049 758 754 577 496 28
BsiEI CG_ry'CG
Cuts at: 104 1204 1250 2458 104
Size: 1100 46 1208 1308
Fragments arranged by size:
1308 1208 1100 46
BsiHKAI G_wGCw'C
Cuts at: 421 548 676 1454 1644 2436 421
Size: 127 128 778 190 792 1647
Fragments arranged by size:
1647 792 778 190 128 127
Bsp1286I G_dGCh'C
Cuts at: 374 421 537 548 676 1287 1380 1454 1644
Size: 47 116 11 128 611 93 74 190
Cuts at: 1644 1706 2436 374
Size: 62 730 1600
Fragments arranged by size:
1600 730 611 190 128 116 93 74
62 47 11
BspEI T'CCGG_A
Cuts at: 2882 2882
Size: 3662
BspHI T'CATG_A
Cuts at: 2838 2838
Size: 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 712 926 1228 1609 712
Size: 214 302 381 2765
Fragments arranged by size:
2765 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1954 2008 3492 3657 50
Size: 1904 54 1484 165 55
Fragments arranged by size:
1904 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1574 1574
Size: 3662
BsrFI r'CCGG_y
Cuts at: 1660 1841 2050 1660
Size: 181 209 3272
Fragments arranged by size:
3272 209 181
BssSI C'ACGA_G
Cuts at: 1933 2291 1933
Size: 358 3304
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1039
Size: 3662
BstBI TT'CG_AA
Cuts at: 2023 2023
Size: 3662
BstYI r'GATC_y
Cuts at: 1 512 687 1154 1177 1512 1758 2770 2856
Size: 511 175 467 23 335 246 1012 86
Cuts at: 2856 2868 3214 1
Size: 12 346 449
Fragments arranged by size:
1012 511 467 449 346 335 246 175
86 23 12
BtgI C'CryG_G
Cuts at: 620 620
Size: 3662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1612 1793 1956 1612
Size: 181 163 3318
Fragments arranged by size:
3318 181 163
BtsI GCAGTG_nn'
Cuts at: 905 905
Size: 3662
DraI TTT'AAA
Cuts at: 661 661
Size: 3662
DrdI GACnn_nn'nnGTC
Cuts at: 1368 2226 1368
Size: 858 2804
EaeI y'GGCC_r
Cuts at: 596 1247 1421 1812 1839 3184 596
Size: 651 174 391 27 1345 1074
Fragments arranged by size:
1345 1074 651 391 174 27
EagI C'GGCC_G
Cuts at: 1247 1247
Size: 3662
EarI CTCTTCn'nnn_
Cuts at: 130 1685 1895 130
Size: 1555 210 1897
Fragments arranged by size:
1897 1555 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 475 1870 2180 2326 3472 475
Size: 1395 310 146 1146 665
Fragments arranged by size:
1395 1146 665 310 146
EcoICRI GAG'CTC
Cuts at: 546 674 546
Size: 128 3534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1487 1919 2666 2934 388
Size: 1099 432 747 268 1116
Fragments arranged by size:
1116 1099 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 636 636
Size: 3662
EcoO109I rG'GnC_Cy
Cuts at: 801 801
Size: 3662
EcoRI G'AATT_C
Cuts at: 666 666
Size: 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1681 1681
Size: 3662
FspI TGC'GCA
Cuts at: 1443 1443
Size: 3662
HaeII r_GCGC'y
Cuts at: 1096 1344 2366 3345 1096
Size: 248 1022 979 1413
Fragments arranged by size:
1413 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 1504 1534 1536 1566 562
Size: 32 910 30 2 30 2658
Fragments arranged by size:
2658 910 32 30 30 2
HincII GTy'rAC
Cuts at: 701 701
Size: 3662
KasI G'GCGC_C
Cuts at: 1092 1340 1092
Size: 248 3414
KpnI G_GTAC'C
Cuts at: 682 682
Size: 3662
MluI A'CGCG_T
Cuts at: 3095 3095
Size: 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 342 2333 2517 342
Size: 1991 184 1487
Fragments arranged by size:
1991 1487 184
MscI TGG'CCA
Cuts at: 598 1423 598
Size: 825 2837
MslI CAynn'nnrTG
Cuts at: 1651 1778 1651
Size: 127 3535
MspA1I CmG'CkG
Cuts at: 183 605 773 1087 1447 2460 2705 183
Size: 422 168 314 360 1013 245 1140
Fragments arranged by size:
1140 1013 422 360 314 245 168
NaeI GCC'GGC
Cuts at: 1843 1843
Size: 3662
NarI GG'CG_CC
Cuts at: 1093 1341 1093
Size: 248 3414
NgoMIV G'CCGG_C
Cuts at: 1841 1841
Size: 3662
NspI r_CATG'y
Cuts at: 715 1215 2122 715
Size: 500 907 2255
Fragments arranged by size:
2255 907 500
PciI A'CATG_T
Cuts at: 2118 2118
Size: 3662
PflMI CCAn_nnn'nTGG
Cuts at: 621 621
Size: 3662
PmeI GTTT'AAAC
Cuts at: 661 661
Size: 3662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 274 306 2801 2833 274
Size: 32 2495 32 1103
Fragments arranged by size:
2495 1103 32 32
PshAI GACnn'nnGTC
Cuts at: 563 563
Size: 3662
PstI C_TGCA'G
Cuts at: 147 709 1394 147
Size: 562 685 2415
Fragments arranged by size:
2415 685 562
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3662
PvuII CAG'CTG
Cuts at: 1087 1447 1087
Size: 360 3302
RsrII CG'GwC_CG
Cuts at: 1857 1857
Size: 3662
SacI G_AGCT'C
Cuts at: 548 676 548
Size: 128 3534
SalI G'TCGA_C
Cuts at: 699 699
Size: 3662
SapI GCTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
SbfI CC_TGCA'GG
Cuts at: 147 709 147
Size: 562 3100
ScaI AGT'ACT
Cuts at: 3179 3179
Size: 3662
SfcI C'TryA_G
Cuts at: 33 143 219 477 705 1390 2383 2574 33
Size: 110 76 258 228 685 993 191 1121
Fragments arranged by size:
1121 993 685 258 228 191 110 76
SfoI GGC'GCC
Cuts at: 1094 1342 1094
Size: 248 3414
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
SmaI CCC'GGG
Cuts at: 684 684
Size: 3662
SmlI C'TyrA_G
Cuts at: 817 1732 2224 2486 817
Size: 915 492 262 1993
Fragments arranged by size:
1993 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3662
SphI G_CATG'C
Cuts at: 715 1215 715
Size: 500 3162
StuI AGG'CCT
Cuts at: 444 444
Size: 3662
StyI C'CwwG_G
Cuts at: 796 1150 796
Size: 354 3308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 229 1245 1378 1837 2046 229
Size: 1016 133 459 209 1845
Fragments arranged by size:
1845 1016 459 209 133
TatI w'GTAC_w
Cuts at: 209 3177 209
Size: 2968 694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 3630
Tth111I GACn'n_nGTC
Cuts at: 202 1459 202
Size: 1257 2405
XbaI T'CTAG_A
Cuts at: 693 693
Size: 3662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1157 1157
Size: 3662
XmaI C'CCGG_G
Cuts at: 682 682
Size: 3662
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI