Restriction map for
pF1KB6878
(Circular) (Six-base) MAPSORT of: pF1KB6878.seq Check: 9652 from: 1 to: 3713
pF1KB6878 3713 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 237 237
Size: 3713
AccI GT'mk_AC
Cuts at: 751 751
Size: 3713
Acc65I G'GTAC_C
Cuts at: 729 729
Size: 3713
AclI AA'CG_TT
Cuts at: 3553 3553
Size: 3713
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1538 1970 2717 2985 193
Size: 1345 432 747 268 921
Fragments arranged by size:
1345 921 747 432 268
AflIII A'CryG_T
Cuts at: 1065 2169 3146 1065
Size: 1104 977 1632
Fragments arranged by size:
1632 1104 977
AgeI A'CCGG_T
Cuts at: 2101 2101
Size: 3713
AhdI GACnn_n'nnGTC
Cuts at: 186 186
Size: 3713
AlwNI CAG_nnn'CTG
Cuts at: 363 420 592 664 1018 2585 363
Size: 57 172 72 354 1567 1491
Fragments arranged by size:
1567 1491 354 172 72 57
ApaLI G'TGCA_C
Cuts at: 2483 2483
Size: 3713
ApoI r'AATT_y
Cuts at: 717 3066 3322 717
Size: 2349 256 1108
Fragments arranged by size:
2349 1108 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3713
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
AvaI C'yCGr_G
Cuts at: 733 733
Size: 3713
BamHI G'GATC_C
Cuts at: 738 738
Size: 3713
BanI G'GyrC_C
Cuts at: 729 1143 1391 1426 729
Size: 414 248 35 3016
Fragments arranged by size:
3016 414 248 35
BanII G_rGCy'C
Cuts at: 126 635 727 1757 126
Size: 509 92 1030 2082
Fragments arranged by size:
2082 1030 509 92
BbeI G_GCGC'C
Cuts at: 1147 1395 1147
Size: 248 3465
BciVI GTATCCnnnnn_n'
Cuts at: 1611 2372 3713 1611
Size: 761 1341 1611
Fragments arranged by size:
1611 1341 761
BclI T'GATC_A
Cuts at: 1233 1233
Size: 3713
BfrBI ATG'CAT
Cuts at: 291 291
Size: 3713
BglII A'GATC_T
Cuts at: 473 1228 3265 473
Size: 755 2037 921
Fragments arranged by size:
2037 921 755
BlpI GC'TnA_GC
Cuts at: 825 825
Size: 3713
Bme1580I G_kGCm'C
Cuts at: 1338 1431 2487 1338
Size: 93 1056 2564
Fragments arranged by size:
2564 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 629 925 1006 1341 3485 629
Size: 296 81 335 2144 857
Fragments arranged by size:
2144 857 335 296 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 528 2080 528
Size: 1552 2161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 517
Size: 3713
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 507 889 1768 2260 2558 337
Size: 170 382 879 492 298 1492
Fragments arranged by size:
1492 879 492 382 298 170
BsaI GGTCTCn'nnnn_
Cuts at: 3417 3417
Size: 3713
BsaAI yAC'GTr
Cuts at: 1066 1066
Size: 3713
BsaBI GATnn'nnATC
Cuts at: 6 1227 3264 6
Size: 1221 2037 455
Fragments arranged by size:
2037 1221 455
BsaHI Gr'CG_yC
Cuts at: 622 1144 1392 2094 3590 622
Size: 522 248 702 1496 745
Fragments arranged by size:
1496 745 702 522 248
BsaWI w'CCGG_w
Cuts at: 446 902 1423 2101 2375 2522 2933 446
Size: 456 521 678 274 147 411 1226
Fragments arranged by size:
1226 678 521 456 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 367 397 460 490 520 367
Size: 30 63 30 30 3560
Fragments arranged by size:
3560 63 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 514 517 451
Size: 63 3 3647
Fragments arranged by size:
3647 63 3
BseYI C'CCAG_C
Cuts at: 113 1138 1896 2473 2969 113
Size: 1025 758 577 496 857
Fragments arranged by size:
1025 857 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 447 1255 1301 2509 104
Size: 343 808 46 1208 1308
Fragments arranged by size:
1308 1208 808 343 46
BsiHKAI G_wGCw'C
Cuts at: 704 727 1505 1695 2487 704
Size: 23 778 190 792 1930
Fragments arranged by size:
1930 792 778 190 23
BsmBI CGTCTCn'nnnn_
Cuts at: 136 235 461 136
Size: 99 226 3388
Fragments arranged by size:
3388 226 99
Bsp1286I G_dGCh'C
Cuts at: 126 635 704 727 1338 1431 1505 1695 1757
Size: 509 69 23 611 93 74 190 62
Cuts at: 1757 2487 126
Size: 730 1352
Fragments arranged by size:
1352 730 611 509 190 93 74 69
62 23
BspEI T'CCGG_A
Cuts at: 2933 2933
Size: 3713
BspHI T'CATG_A
Cuts at: 2889 2889
Size: 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 237 554 763 977 1279 1660 237
Size: 317 209 214 302 381 2290
Fragments arranged by size:
2290 381 317 302 214 209
BsrBI CCG'CTC
Cuts at: 50 2005 2059 3543 3708 50
Size: 1955 54 1484 165 55
Fragments arranged by size:
1955 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1625 1625
Size: 3713
BsrFI r'CCGG_y
Cuts at: 319 1711 1892 2101 319
Size: 1392 181 209 1931
Fragments arranged by size:
1931 1392 209 181
BssSI C'ACGA_G
Cuts at: 1984 2342 1984
Size: 358 3355
BstAPI GCAn_nnn'nTGC
Cuts at: 664 1090 664
Size: 426 3287
BstBI TT'CG_AA
Cuts at: 2074 2074
Size: 3713
BstXI CCAn_nnnn'nTGG
Cuts at: 682 682
Size: 3713
BstYI r'GATC_y
Cuts at: 1 473 738 1205 1228 1563 1809 2821 2907
Size: 472 265 467 23 335 246 1012 86
Cuts at: 2907 2919 3265 1
Size: 12 346 449
Fragments arranged by size:
1012 472 467 449 346 335 265 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1663 1844 2007 1663
Size: 181 163 3369
Fragments arranged by size:
3369 181 163
BtsI GCAGTG_nn'
Cuts at: 427 956 427
Size: 529 3184
DraI TTT'AAA
Cuts at: 712 712
Size: 3713
DrdI GACnn_nn'nnGTC
Cuts at: 249 1419 2277 249
Size: 1170 858 1685
Fragments arranged by size:
1685 1170 858
EaeI y'GGCC_r
Cuts at: 1298 1472 1863 1890 3235 1298
Size: 174 391 27 1345 1776
Fragments arranged by size:
1776 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1298 1298
Size: 3713
EarI CTCTTCn'nnn_
Cuts at: 1736 1946 1736
Size: 210 3503
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1921 2231 2377 3523 1921
Size: 310 146 1146 2111
Fragments arranged by size:
2111 1146 310 146
EcoICRI GAG'CTC
Cuts at: 725 725
Size: 3713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 517 1538 1970 2717 2985 193
Size: 324 1021 432 747 268 921
Fragments arranged by size:
1021 921 747 432 324 268
EcoO109I rG'GnC_Cy
Cuts at: 852 852
Size: 3713
EcoRI G'AATT_C
Cuts at: 717 717
Size: 3713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1732 1732
Size: 3713
FspI TGC'GCA
Cuts at: 424 1494 424
Size: 1070 2643
HaeII r_GCGC'y
Cuts at: 1147 1395 2417 3396 1147
Size: 248 1022 979 1464
Fragments arranged by size:
1464 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 172 204 367 399 458 460 490 492 574
Size: 32 163 32 59 2 30 2 82
Cuts at: 574 589 606 621 1555 1585 1587 1617 172
Size: 15 17 15 934 30 2 30 2268
Fragments arranged by size:
2268 934 163 82 59 32 32 30
30 30 17 15 15 2 2 2
HincII GTy'rAC
Cuts at: 752 752
Size: 3713
HindIII A'AGCT_T
Cuts at: 560 560
Size: 3713
KasI G'GCGC_C
Cuts at: 1143 1391 1143
Size: 248 3465
KpnI G_GTAC'C
Cuts at: 733 733
Size: 3713
MluI A'CGCG_T
Cuts at: 3146 3146
Size: 3713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 371 2384 2568 371
Size: 2013 184 1516
Fragments arranged by size:
2013 1516 184
MscI TGG'CCA
Cuts at: 1474 1474
Size: 3713
MslI CAynn'nnrTG
Cuts at: 674 680 1702 1829 674
Size: 6 1022 127 2558
Fragments arranged by size:
2558 1022 127 6
MspA1I CmG'CkG
Cuts at: 420 824 1138 1498 2511 2756 420
Size: 404 314 360 1013 245 1377
Fragments arranged by size:
1377 1013 404 360 314 245
NaeI GCC'GGC
Cuts at: 321 1894 321
Size: 1573 2140
NarI GG'CG_CC
Cuts at: 1144 1392 1144
Size: 248 3465
NcoI C'CATG_G
Cuts at: 107 107
Size: 3713
NgoMIV G'CCGG_C
Cuts at: 319 1892 319
Size: 1573 2140
NsiI A_TGCA'T
Cuts at: 293 293
Size: 3713
NspI r_CATG'y
Cuts at: 295 766 1266 2173 295
Size: 471 500 907 1835
Fragments arranged by size:
1835 907 500 471
PciI A'CATG_T
Cuts at: 2169 2169
Size: 3713
PmeI GTTT'AAAC
Cuts at: 712 712
Size: 3713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2852 2884 2852
Size: 32 3681
PstI C_TGCA'G
Cuts at: 155 434 760 1445 155
Size: 279 326 685 2423
Fragments arranged by size:
2423 685 326 279
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3713
PvuII CAG'CTG
Cuts at: 420 1138 1498 420
Size: 718 360 2635
Fragments arranged by size:
2635 718 360
RsrII CG'GwC_CG
Cuts at: 1908 1908
Size: 3713
SacI G_AGCT'C
Cuts at: 727 727
Size: 3713
SalI G'TCGA_C
Cuts at: 750 750
Size: 3713
SapI GCTCTTCn'nnn_
Cuts at: 1736 1946 1736
Size: 210 3503
SbfI CC_TGCA'GG
Cuts at: 760 760
Size: 3713
ScaI AGT'ACT
Cuts at: 3230 3230
Size: 3713
SfcI C'TryA_G
Cuts at: 33 151 430 649 756 1441 2434 2625 33
Size: 118 279 219 107 685 993 191 1121
Fragments arranged by size:
1121 993 685 279 219 191 118 107
SfoI GGC'GCC
Cuts at: 1145 1393 1145
Size: 248 3465
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3713
SmaI CCC'GGG
Cuts at: 735 735
Size: 3713
SmlI C'TyrA_G
Cuts at: 352 522 868 1783 2275 2537 352
Size: 170 346 915 492 262 1528
Fragments arranged by size:
1528 915 492 346 262 170
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3713
SphI G_CATG'C
Cuts at: 766 1266 766
Size: 500 3213
StyI C'CwwG_G
Cuts at: 107 847 1201 107
Size: 740 354 2619
Fragments arranged by size:
2619 740 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1296 1429 1888 2097 1296
Size: 133 459 209 2912
Fragments arranged by size:
2912 459 209 133
TatI w'GTAC_w
Cuts at: 3228 3228
Size: 3713
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 3681
Tth111I GACn'n_nGTC
Cuts at: 1510 1510
Size: 3713
XbaI T'CTAG_A
Cuts at: 744 744
Size: 3713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 676 1208 676
Size: 532 3181
XmaI C'CCGG_G
Cuts at: 733 733
Size: 3713
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI