Restriction map for
pF1KB6857
(Circular) (Six-base) MAPSORT of: pF1KB6857.seq Check: 3465 from: 1 to: 3650
pF1KB6857 3650 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 688 688
Size: 3650
Acc65I G'GTAC_C
Cuts at: 666 666
Size: 3650
AclI AA'CG_TT
Cuts at: 3490 3490
Size: 3650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 471 1475 1907 2654 2922 442
Size: 29 1004 432 747 268 1170
Fragments arranged by size:
1170 1004 747 432 268 29
AflIII A'CryG_T
Cuts at: 1002 2106 3083 1002
Size: 1104 977 1569
Fragments arranged by size:
1569 1104 977
AgeI A'CCGG_T
Cuts at: 2038 2038
Size: 3650
AlwNI CAG_nnn'CTG
Cuts at: 414 955 2522 414
Size: 541 1567 1542
Fragments arranged by size:
1567 1542 541
ApaLI G'TGCA_C
Cuts at: 2420 2420
Size: 3650
ApoI r'AATT_y
Cuts at: 654 3003 3259 654
Size: 2349 256 1045
Fragments arranged by size:
2349 1045 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
AvaI C'yCGr_G
Cuts at: 670 670
Size: 3650
BamHI G'GATC_C
Cuts at: 675 675
Size: 3650
BanI G'GyrC_C
Cuts at: 666 1080 1328 1363 666
Size: 414 248 35 2953
Fragments arranged by size:
2953 414 248 35
BanII G_rGCy'C
Cuts at: 664 1694 664
Size: 1030 2620
BbeI G_GCGC'C
Cuts at: 1084 1332 1084
Size: 248 3402
BciVI GTATCCnnnnn_n'
Cuts at: 1548 2309 3650 1548
Size: 761 1341 1548
Fragments arranged by size:
1548 1341 761
BclI T'GATC_A
Cuts at: 1170 1170
Size: 3650
BglII A'GATC_T
Cuts at: 1165 3202 1165
Size: 2037 1613
BlpI GC'TnA_GC
Cuts at: 762 762
Size: 3650
Bme1580I G_kGCm'C
Cuts at: 1275 1368 2424 1275
Size: 93 1056 2501
Fragments arranged by size:
2501 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 862 943 1278 3422 862
Size: 81 335 2144 1090
Fragments arranged by size:
2144 1090 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 391 610 370
Size: 21 219 3410
Fragments arranged by size:
3410 219 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1705 2197 2495 826
Size: 879 492 298 1981
Fragments arranged by size:
1981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3354 3354
Size: 3650
BsaAI yAC'GTr
Cuts at: 1003 1003
Size: 3650
BsaBI GATnn'nnATC
Cuts at: 6 1164 3201 6
Size: 1158 2037 455
Fragments arranged by size:
2037 1158 455
BsaHI Gr'CG_yC
Cuts at: 1081 1329 2031 3527 1081
Size: 248 702 1496 1204
Fragments arranged by size:
1496 1204 702 248
BsaWI w'CCGG_w
Cuts at: 839 1360 2038 2312 2459 2870 839
Size: 521 678 274 147 411 1619
Fragments arranged by size:
1619 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 487 367
Size: 120 3530
BseYI C'CCAG_C
Cuts at: 1075 1833 2410 2906 1075
Size: 758 577 496 1819
Fragments arranged by size:
1819 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1192 1238 2446 104
Size: 1088 46 1208 1308
Fragments arranged by size:
1308 1208 1088 46
BsiHKAI G_wGCw'C
Cuts at: 664 1442 1632 2424 664
Size: 778 190 792 1890
Fragments arranged by size:
1890 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 664 1275 1368 1442 1632 1694 2424 664
Size: 611 93 74 190 62 730 1890
Fragments arranged by size:
1890 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2870 2870
Size: 3650
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 700 914 1216 1597 700
Size: 214 302 381 2753
Fragments arranged by size:
2753 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1942 1996 3480 3645 50
Size: 1892 54 1484 165 55
Fragments arranged by size:
1892 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 128 1562 128
Size: 1434 2216
BsrFI r'CCGG_y
Cuts at: 1648 1829 2038 1648
Size: 181 209 3260
Fragments arranged by size:
3260 209 181
BssSI C'ACGA_G
Cuts at: 442 1921 2279 442
Size: 1479 358 1813
Fragments arranged by size:
1813 1479 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1027 1027
Size: 3650
BstBI TT'CG_AA
Cuts at: 2011 2011
Size: 3650
BstYI r'GATC_y
Cuts at: 1 356 675 1142 1165 1500 1746 2758 2844
Size: 355 319 467 23 335 246 1012 86
Cuts at: 2844 2856 3202 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 355 346 335 319 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1600 1781 1944 1600
Size: 181 163 3306
Fragments arranged by size:
3306 181 163
BtsI GCAGTG_nn'
Cuts at: 893 893
Size: 3650
DraI TTT'AAA
Cuts at: 649 649
Size: 3650
DrdI GACnn_nn'nnGTC
Cuts at: 1356 2214 1356
Size: 858 2792
EaeI y'GGCC_r
Cuts at: 258 1235 1409 1800 1827 3172 258
Size: 977 174 391 27 1345 736
Fragments arranged by size:
1345 977 736 391 174 27
EagI C'GGCC_G
Cuts at: 1235 1235
Size: 3650
EarI CTCTTCn'nnn_
Cuts at: 427 1673 1883 427
Size: 1246 210 2194
Fragments arranged by size:
2194 1246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 514 1858 2168 2314 3460 514
Size: 1344 310 146 1146 704
Fragments arranged by size:
1344 1146 704 310 146
EcoICRI GAG'CTC
Cuts at: 662 662
Size: 3650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 391 442 471 610 1475 1907 2654 2922
Size: 21 51 29 139 865 432 747 268
Cuts at: 2922 370
Size: 1098
Fragments arranged by size:
1098 865 747 432 268 139 51 29
21
EcoNI CCTnn'n_nnAGG
Cuts at: 460 460
Size: 3650
EcoO109I rG'GnC_Cy
Cuts at: 789 789
Size: 3650
EcoRI G'AATT_C
Cuts at: 654 654
Size: 3650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1669 1669
Size: 3650
FspI TGC'GCA
Cuts at: 610 1431 610
Size: 821 2829
HaeII r_GCGC'y
Cuts at: 1084 1332 2354 3333 1084
Size: 248 1022 979 1401
Fragments arranged by size:
1401 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1492 1522 1524 1554 1492
Size: 30 2 30 3588
Fragments arranged by size:
3588 30 30 2
HincII GTy'rAC
Cuts at: 689 689
Size: 3650
KasI G'GCGC_C
Cuts at: 1080 1328 1080
Size: 248 3402
KpnI G_GTAC'C
Cuts at: 670 670
Size: 3650
MfeI C'AATT_G
Cuts at: 421 421
Size: 3650
MluI A'CGCG_T
Cuts at: 3083 3083
Size: 3650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2505 2321
Size: 184 3466
MscI TGG'CCA
Cuts at: 260 1411 260
Size: 1151 2499
MslI CAynn'nnrTG
Cuts at: 1639 1766 1639
Size: 127 3523
MspA1I CmG'CkG
Cuts at: 761 1075 1435 2448 2693 761
Size: 314 360 1013 245 1718
Fragments arranged by size:
1718 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1831 1831
Size: 3650
NarI GG'CG_CC
Cuts at: 1081 1329 1081
Size: 248 3402
NcoI C'CATG_G
Cuts at: 107 107
Size: 3650
NgoMIV G'CCGG_C
Cuts at: 1829 1829
Size: 3650
NspI r_CATG'y
Cuts at: 598 703 1203 2110 598
Size: 105 500 907 2138
Fragments arranged by size:
2138 907 500 105
PciI A'CATG_T
Cuts at: 2106 2106
Size: 3650
PmeI GTTT'AAAC
Cuts at: 649 649
Size: 3650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 3618
PstI C_TGCA'G
Cuts at: 381 581 697 1382 381
Size: 200 116 685 2649
Fragments arranged by size:
2649 685 200 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3650
PvuII CAG'CTG
Cuts at: 1075 1435 1075
Size: 360 3290
RsrII CG'GwC_CG
Cuts at: 1845 1845
Size: 3650
SacI G_AGCT'C
Cuts at: 664 664
Size: 3650
SalI G'TCGA_C
Cuts at: 687 687
Size: 3650
SapI GCTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
SbfI CC_TGCA'GG
Cuts at: 697 697
Size: 3650
ScaI AGT'ACT
Cuts at: 3167 3167
Size: 3650
SfcI C'TryA_G
Cuts at: 33 377 577 693 1378 2371 2562 33
Size: 344 200 116 685 993 191 1121
Fragments arranged by size:
1121 993 685 344 200 191 116
SfoI GGC'GCC
Cuts at: 1082 1330 1082
Size: 248 3402
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
SmaI CCC'GGG
Cuts at: 672 672
Size: 3650
SmlI C'TyrA_G
Cuts at: 805 1720 2212 2474 805
Size: 915 492 262 1981
Fragments arranged by size:
1981 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3650
SphI G_CATG'C
Cuts at: 598 703 1203 598
Size: 105 500 3045
Fragments arranged by size:
3045 500 105
StyI C'CwwG_G
Cuts at: 107 784 1138 107
Size: 677 354 2619
Fragments arranged by size:
2619 677 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1233 1366 1825 2034 1233
Size: 133 459 209 2849
Fragments arranged by size:
2849 459 209 133
TatI w'GTAC_w
Cuts at: 3165 3165
Size: 3650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3618
Tth111I GACn'n_nGTC
Cuts at: 1447 1447
Size: 3650
XbaI T'CTAG_A
Cuts at: 681 681
Size: 3650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1145 1145
Size: 3650
XmaI C'CCGG_G
Cuts at: 670 670
Size: 3650
XmnI GAAnn'nnTTC
Cuts at: 455 455
Size: 3650
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI