Restriction map for
pF1KB6846
(Circular) (Six-base) MAPSORT of: pF1KB6846.seq Check: 2775 from: 1 to: 3692
pF1KB6846 3692 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 620 730 620
Size: 110 3582
Acc65I G'GTAC_C
Cuts at: 230 708 230
Size: 478 3214
AclI AA'CG_TT
Cuts at: 3532 3532
Size: 3692
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1517 1949 2696 2964 667
Size: 850 432 747 268 1395
Fragments arranged by size:
1395 850 747 432 268
AflIII A'CryG_T
Cuts at: 1044 2148 3125 1044
Size: 1104 977 1611
Fragments arranged by size:
1611 1104 977
AgeI A'CCGG_T
Cuts at: 2080 2080
Size: 3692
AlwNI CAG_nnn'CTG
Cuts at: 387 426 997 2564 387
Size: 39 571 1567 1515
Fragments arranged by size:
1567 1515 571 39
ApaLI G'TGCA_C
Cuts at: 115 2462 115
Size: 2347 1345
ApoI r'AATT_y
Cuts at: 696 3045 3301 696
Size: 2349 256 1087
Fragments arranged by size:
2349 1087 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3692
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
AvaI C'yCGr_G
Cuts at: 712 712
Size: 3692
BamHI G'GATC_C
Cuts at: 717 717
Size: 3692
BanI G'GyrC_C
Cuts at: 187 230 708 1122 1370 1405 187
Size: 43 478 414 248 35 2474
Fragments arranged by size:
2474 478 414 248 43 35
BanII G_rGCy'C
Cuts at: 458 522 531 706 1736 458
Size: 64 9 175 1030 2414
Fragments arranged by size:
2414 1030 175 64 9
BbeI G_GCGC'C
Cuts at: 191 1126 1374 191
Size: 935 248 2509
Fragments arranged by size:
2509 935 248
BbvCI CC'TCA_GC
Cuts at: 564 564
Size: 3692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 109 143 109
Size: 34 3658
BciVI GTATCCnnnnn_n'
Cuts at: 1590 2351 3692 1590
Size: 761 1341 1590
Fragments arranged by size:
1590 1341 761
BclI T'GATC_A
Cuts at: 1212 1212
Size: 3692
BglI GCCn_nnn'nGGC
Cuts at: 250 250
Size: 3692
BglII A'GATC_T
Cuts at: 1207 3244 1207
Size: 2037 1655
BlpI GC'TnA_GC
Cuts at: 804 804
Size: 3692
Bme1580I G_kGCm'C
Cuts at: 119 1317 1410 2466 119
Size: 1198 93 1056 1345
Fragments arranged by size:
1345 1198 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 172 904 985 1320 3464 172
Size: 732 81 335 2144 400
Fragments arranged by size:
2144 732 400 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 3692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 536 244
Size: 292 3400
Bpu10I CC'TnA_GC
Cuts at: 564 564
Size: 3692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1747 2239 2537 868
Size: 879 492 298 2023
Fragments arranged by size:
2023 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3396 3396
Size: 3692
BsaAI yAC'GTr
Cuts at: 1045 1045
Size: 3692
BsaBI GATnn'nnATC
Cuts at: 6 1206 3243 6
Size: 1200 2037 455
Fragments arranged by size:
2037 1200 455
BsaHI Gr'CG_yC
Cuts at: 188 1123 1371 2073 3569 188
Size: 935 248 702 1496 311
Fragments arranged by size:
1496 935 702 311 248
BsaWI w'CCGG_w
Cuts at: 881 1402 2080 2354 2501 2912 881
Size: 521 678 274 147 411 1661
Fragments arranged by size:
1661 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 443 1117 1875 2452 2948 443
Size: 674 758 577 496 1187
Fragments arranged by size:
1187 758 674 577 496
BsiEI CG_ry'CG
Cuts at: 104 1234 1280 2488 104
Size: 1130 46 1208 1308
Fragments arranged by size:
1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 119 706 1484 1674 2466 119
Size: 587 778 190 792 1345
Fragments arranged by size:
1345 792 778 587 190
Bsp1286I G_dGCh'C
Cuts at: 119 458 522 531 706 1317 1410 1484 1674
Size: 339 64 9 175 611 93 74 190
Cuts at: 1674 1736 2466 119
Size: 62 730 1345
Fragments arranged by size:
1345 730 611 339 190 175 93 74
64 62 9
BspEI T'CCGG_A
Cuts at: 2912 2912
Size: 3692
BspHI T'CATG_A
Cuts at: 2868 2868
Size: 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 742 956 1258 1639 742
Size: 214 302 381 2795
Fragments arranged by size:
2795 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1984 2038 3522 3687 50
Size: 1934 54 1484 165 55
Fragments arranged by size:
1934 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1604 1604
Size: 3692
BsrFI r'CCGG_y
Cuts at: 1690 1871 2080 1690
Size: 181 209 3302
Fragments arranged by size:
3302 209 181
BsrGI T'GTAC_A
Cuts at: 653 653
Size: 3692
BssSI C'ACGA_G
Cuts at: 1963 2321 1963
Size: 358 3334
BstAPI GCAn_nnn'nTGC
Cuts at: 1069 1069
Size: 3692
BstBI TT'CG_AA
Cuts at: 2053 2053
Size: 3692
BstYI r'GATC_y
Cuts at: 1 717 1184 1207 1542 1788 2800 2886 2898
Size: 716 467 23 335 246 1012 86 12
Cuts at: 2898 3244 1
Size: 346 449
Fragments arranged by size:
1012 716 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 448 107
Size: 341 3351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1642 1823 1986 1642
Size: 181 163 3348
Fragments arranged by size:
3348 181 163
BtsI GCAGTG_nn'
Cuts at: 282 587 935 282
Size: 305 348 3039
Fragments arranged by size:
3039 348 305
DraI TTT'AAA
Cuts at: 691 691
Size: 3692
DrdI GACnn_nn'nnGTC
Cuts at: 217 1398 2256 217
Size: 1181 858 1653
Fragments arranged by size:
1653 1181 858
EaeI y'GGCC_r
Cuts at: 1277 1451 1842 1869 3214 1277
Size: 174 391 27 1345 1755
Fragments arranged by size:
1755 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1277 1277
Size: 3692
EarI CTCTTCn'nnn_
Cuts at: 337 444 616 1715 1925 337
Size: 107 172 1099 210 2104
Fragments arranged by size:
2104 1099 210 172 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1900 2210 2356 3502 1900
Size: 310 146 1146 2090
Fragments arranged by size:
2090 1146 310 146
EcoICRI GAG'CTC
Cuts at: 704 704
Size: 3692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 536 667 1517 1949 2696 2964 244
Size: 292 131 850 432 747 268 972
Fragments arranged by size:
972 850 747 432 292 268 131
EcoNI CCTnn'n_nnAGG
Cuts at: 238 342 238
Size: 104 3588
EcoO109I rG'GnC_Cy
Cuts at: 384 423 831 384
Size: 39 408 3245
Fragments arranged by size:
3245 408 39
EcoRI G'AATT_C
Cuts at: 696 696
Size: 3692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1711 1711
Size: 3692
FspI TGC'GCA
Cuts at: 1473 1473
Size: 3692
HaeII r_GCGC'y
Cuts at: 191 1126 1374 2396 3375 191
Size: 935 248 1022 979 508
Fragments arranged by size:
1022 979 935 508 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 1534 1564 1566 1596 547
Size: 32 955 30 2 30 2643
Fragments arranged by size:
2643 955 32 30 30 2
HincII GTy'rAC
Cuts at: 435 731 435
Size: 296 3396
KasI G'GCGC_C
Cuts at: 187 1122 1370 187
Size: 935 248 2509
Fragments arranged by size:
2509 935 248
KpnI G_GTAC'C
Cuts at: 234 712 234
Size: 478 3214
MluI A'CGCG_T
Cuts at: 3125 3125
Size: 3692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 380 2363 2547 380
Size: 1983 184 1525
Fragments arranged by size:
1983 1525 184
MscI TGG'CCA
Cuts at: 1453 1453
Size: 3692
MslI CAynn'nnrTG
Cuts at: 112 1681 1808 112
Size: 1569 127 1996
Fragments arranged by size:
1996 1569 127
MspA1I CmG'CkG
Cuts at: 465 568 803 1117 1477 2490 2735 465
Size: 103 235 314 360 1013 245 1422
Fragments arranged by size:
1422 1013 360 314 245 235 103
NaeI GCC'GGC
Cuts at: 1873 1873
Size: 3692
NarI GG'CG_CC
Cuts at: 188 1123 1371 188
Size: 935 248 2509
Fragments arranged by size:
2509 935 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3692
NgoMIV G'CCGG_C
Cuts at: 1871 1871
Size: 3692
NspI r_CATG'y
Cuts at: 472 745 1245 2152 472
Size: 273 500 907 2012
Fragments arranged by size:
2012 907 500 273
PciI A'CATG_T
Cuts at: 2148 2148
Size: 3692
PfoI T'CCnGG_A
Cuts at: 221 221
Size: 3692
PmeI GTTT'AAAC
Cuts at: 691 691
Size: 3692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2831 2863 2831
Size: 32 3660
PstI C_TGCA'G
Cuts at: 289 464 673 739 1424 289
Size: 175 209 66 685 2557
Fragments arranged by size:
2557 685 209 175 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3692
PvuII CAG'CTG
Cuts at: 465 568 1117 1477 465
Size: 103 549 360 2680
Fragments arranged by size:
2680 549 360 103
RsrII CG'GwC_CG
Cuts at: 1887 1887
Size: 3692
SacI G_AGCT'C
Cuts at: 706 706
Size: 3692
SalI G'TCGA_C
Cuts at: 729 729
Size: 3692
SapI GCTCTTCn'nnn_
Cuts at: 1715 1925 1715
Size: 210 3482
SbfI CC_TGCA'GG
Cuts at: 673 739 673
Size: 66 3626
ScaI AGT'ACT
Cuts at: 3209 3209
Size: 3692
SfcI C'TryA_G
Cuts at: 33 285 460 669 735 1420 2413 2604 33
Size: 252 175 209 66 685 993 191 1121
Fragments arranged by size:
1121 993 685 252 209 191 175 66
SfiI GGCCn_nnn'nGGCC
Cuts at: 250 250
Size: 3692
SfoI GGC'GCC
Cuts at: 189 1124 1372 189
Size: 935 248 2509
Fragments arranged by size:
2509 935 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3692
SmaI CCC'GGG
Cuts at: 714 714
Size: 3692
SmlI C'TyrA_G
Cuts at: 847 1762 2254 2516 847
Size: 915 492 262 2023
Fragments arranged by size:
2023 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3692
SphI G_CATG'C
Cuts at: 745 1245 745
Size: 500 3192
StuI AGG'CCT
Cuts at: 667 667
Size: 3692
StyI C'CwwG_G
Cuts at: 107 245 826 1180 107
Size: 138 581 354 2619
Fragments arranged by size:
2619 581 354 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 1408 1867 2076 1275
Size: 133 459 209 2891
Fragments arranged by size:
2891 459 209 133
TatI w'GTAC_w
Cuts at: 653 3207 653
Size: 2554 1138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 3660
Tth111I GACn'n_nGTC
Cuts at: 1489 1489
Size: 3692
XbaI T'CTAG_A
Cuts at: 723 723
Size: 3692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1187 1187
Size: 3692
XmaI C'CCGG_G
Cuts at: 712 712
Size: 3692
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BfrBI BmgBI BmtI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI