Restriction map for
pF1KB6843
(Circular) (Six-base) MAPSORT of: pF1KB6843.seq Check: 7381 from: 1 to: 3638
pF1KB6843 3638 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 676 676
Size: 3638
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 3478 3478
Size: 3638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AlwNI CAG_nnn'CTG
Cuts at: 552 943 2510 552
Size: 391 1567 1680
Fragments arranged by size:
1680 1567 391
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 642 2991 3247 642
Size: 2349 256 1033
Fragments arranged by size:
2349 1033 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 172 658 172
Size: 486 3152
BamHI G'GATC_C
Cuts at: 663 663
Size: 3638
BanI G'GyrC_C
Cuts at: 237 654 1068 1316 1351 237
Size: 417 414 248 35 2524
Fragments arranged by size:
2524 417 414 248 35
BanII G_rGCy'C
Cuts at: 652 1682 652
Size: 1030 2608
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3604
BciVI GTATCCnnnnn_n'
Cuts at: 1536 2297 3638 1536
Size: 761 1341 1536
Fragments arranged by size:
1536 1341 761
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglI GCCn_nnn'nGGC
Cuts at: 161 466 161
Size: 305 3333
BglII A'GATC_T
Cuts at: 1153 3190 1153
Size: 2037 1601
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 1263 1356 2412 1263
Size: 93 1056 2489
Fragments arranged by size:
2489 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 419 850 931 1266 3410 419
Size: 431 81 335 2144 647
Fragments arranged by size:
2144 647 431 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1693 2185 2483 814
Size: 879 492 298 1969
Fragments arranged by size:
1969 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3342 3342
Size: 3638
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 1152 3189 6
Size: 1146 2037 455
Fragments arranged by size:
2037 1146 455
BsaHI Gr'CG_yC
Cuts at: 1069 1317 2019 3515 1069
Size: 248 702 1496 1192
Fragments arranged by size:
1496 1192 702 248
BsaWI w'CCGG_w
Cuts at: 827 1348 2026 2300 2447 2858 827
Size: 521 678 274 147 411 1607
Fragments arranged by size:
1607 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 589 619 589
Size: 30 3608
BseYI C'CCAG_C
Cuts at: 300 1063 1821 2398 2894 300
Size: 763 758 577 496 1044
Fragments arranged by size:
1044 763 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 122 1180 1226 2434 104
Size: 18 1058 46 1208 1308
Fragments arranged by size:
1308 1208 1058 46 18
BsiHKAI G_wGCw'C
Cuts at: 652 1430 1620 2412 652
Size: 778 190 792 1878
Fragments arranged by size:
1878 792 778 190
BsmI GAATG_Cn'
Cuts at: 431 431
Size: 3638
Bsp1286I G_dGCh'C
Cuts at: 652 1263 1356 1430 1620 1682 2412 652
Size: 611 93 74 190 62 730 1878
Fragments arranged by size:
1878 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2858 2858
Size: 3638
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 902 1204 1585 688
Size: 214 302 381 2741
Fragments arranged by size:
2741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1930 1984 3468 3633 50
Size: 1880 54 1484 165 55
Fragments arranged by size:
1880 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 1636 1817 2026 1636
Size: 181 209 3248
Fragments arranged by size:
3248 209 181
BssSI C'ACGA_G
Cuts at: 1909 2267 1909
Size: 358 3280
BstAPI GCAn_nnn'nTGC
Cuts at: 561 1015 561
Size: 454 3184
BstBI TT'CG_AA
Cuts at: 1999 1999
Size: 3638
BstXI CCAn_nnnn'nTGG
Cuts at: 330 330
Size: 3638
BstYI r'GATC_y
Cuts at: 1 434 663 1130 1153 1488 1734 2746 2832
Size: 433 229 467 23 335 246 1012 86
Cuts at: 2832 2844 3190 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 433 346 335 246 229
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 574 574
Size: 3638
BtgI C'CryG_G
Cuts at: 138 241 138
Size: 103 3535
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1588 1769 1932 1588
Size: 181 163 3294
Fragments arranged by size:
3294 181 163
BtsI GCAGTG_nn'
Cuts at: 476 881 476
Size: 405 3233
DraI TTT'AAA
Cuts at: 637 637
Size: 3638
DrdI GACnn_nn'nnGTC
Cuts at: 1344 2202 1344
Size: 858 2780
EaeI y'GGCC_r
Cuts at: 141 1223 1397 1788 1815 3160 141
Size: 1082 174 391 27 1345 619
Fragments arranged by size:
1345 1082 619 391 174 27
EagI C'GGCC_G
Cuts at: 1223 1223
Size: 3638
EarI CTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1846 2156 2302 3448 1846
Size: 310 146 1146 2036
Fragments arranged by size:
2036 1146 310 146
EcoICRI GAG'CTC
Cuts at: 650 650
Size: 3638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 570 777 570
Size: 207 3431
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1657 1657
Size: 3638
FspI TGC'GCA
Cuts at: 328 1419 328
Size: 1091 2547
FspAI rTGC'GCAy
Cuts at: 328 328
Size: 3638
HaeII r_GCGC'y
Cuts at: 1072 1320 2342 3321 1072
Size: 248 1022 979 1389
Fragments arranged by size:
1389 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 426 438 458 470 1480 1510 1512 1542 426
Size: 12 20 12 1010 30 2 30 2522
Fragments arranged by size:
2522 1010 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 677 677
Size: 3638
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2493 2309
Size: 184 3454
MscI TGG'CCA
Cuts at: 1399 1399
Size: 3638
MslI CAynn'nnrTG
Cuts at: 328 424 1627 1754 328
Size: 96 1203 127 2212
Fragments arranged by size:
2212 1203 127 96
MspA1I CmG'CkG
Cuts at: 549 749 1063 1423 2436 2681 549
Size: 200 314 360 1013 245 1506
Fragments arranged by size:
1506 1013 360 314 245 200
NaeI GCC'GGC
Cuts at: 1819 1819
Size: 3638
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NcoI C'CATG_G
Cuts at: 138 241 138
Size: 103 3535
NgoMIV G'CCGG_C
Cuts at: 1817 1817
Size: 3638
NspI r_CATG'y
Cuts at: 691 1191 2098 691
Size: 500 907 2231
Fragments arranged by size:
2231 907 500
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 3606
PpuMI rG'GwC_Cy
Cuts at: 570 570
Size: 3638
PstI C_TGCA'G
Cuts at: 129 434 557 685 1370 129
Size: 305 123 128 685 2397
Fragments arranged by size:
2397 685 305 128 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 549 1063 1423 549
Size: 514 360 2764
Fragments arranged by size:
2764 514 360
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 652 652
Size: 3638
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
SbfI CC_TGCA'GG
Cuts at: 685 685
Size: 3638
ScaI AGT'ACT
Cuts at: 383 3155 383
Size: 2772 866
SfcI C'TryA_G
Cuts at: 33 125 430 553 681 1366 2359 2550 33
Size: 92 305 123 128 685 993 191 1121
Fragments arranged by size:
1121 993 685 305 191 128 123 92
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 660 660
Size: 3638
SmlI C'TyrA_G
Cuts at: 793 1708 2200 2462 793
Size: 915 492 262 1969
Fragments arranged by size:
1969 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
StyI C'CwwG_G
Cuts at: 138 241 772 1126 138
Size: 103 531 354 2650
Fragments arranged by size:
2650 531 354 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 108 157 1221 1354 1813 2022 108
Size: 49 1064 133 459 209 1724
Fragments arranged by size:
1724 1064 459 209 133 49
TatI w'GTAC_w
Cuts at: 381 3153 381
Size: 2772 866
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3606
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 669 669
Size: 3638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1133 1133
Size: 3638
XmaI C'CCGG_G
Cuts at: 658 658
Size: 3638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI
BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI