Restriction map for
pF1KB6839
(Circular) (Six-base) MAPSORT of: pF1KB6839.seq Check: 4562 from: 1 to: 3629
pF1KB6839 3629 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 667 667
Size: 3629
Acc65I G'GTAC_C
Cuts at: 645 645
Size: 3629
AclI AA'CG_TT
Cuts at: 3469 3469
Size: 3629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1454 1886 2633 2901 431
Size: 1023 432 747 268 1159
Fragments arranged by size:
1159 1023 747 432 268
AflIII A'CryG_T
Cuts at: 981 2085 3062 981
Size: 1104 977 1548
Fragments arranged by size:
1548 1104 977
AgeI A'CCGG_T
Cuts at: 2017 2017
Size: 3629
AleI CACnn'nnGTG
Cuts at: 412 412
Size: 3629
AlwNI CAG_nnn'CTG
Cuts at: 934 2501 934
Size: 1567 2062
ApaLI G'TGCA_C
Cuts at: 2399 2399
Size: 3629
ApoI r'AATT_y
Cuts at: 353 633 2982 3238 353
Size: 280 2349 256 744
Fragments arranged by size:
2349 744 280 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
AvaI C'yCGr_G
Cuts at: 271 649 271
Size: 378 3251
BamHI G'GATC_C
Cuts at: 654 654
Size: 3629
BanI G'GyrC_C
Cuts at: 562 645 1059 1307 1342 562
Size: 83 414 248 35 2849
Fragments arranged by size:
2849 414 248 83 35
BanII G_rGCy'C
Cuts at: 272 607 643 1673 272
Size: 335 36 1030 2228
Fragments arranged by size:
2228 1030 335 36
BbeI G_GCGC'C
Cuts at: 1063 1311 1063
Size: 248 3381
BbsI GAAGACnn'nnnn_
Cuts at: 428 482 428
Size: 54 3575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 552 586 552
Size: 34 3595
BciVI GTATCCnnnnn_n'
Cuts at: 1527 2288 3629 1527
Size: 761 1341 1527
Fragments arranged by size:
1527 1341 761
BclI T'GATC_A
Cuts at: 1149 1149
Size: 3629
BglII A'GATC_T
Cuts at: 1144 3181 1144
Size: 2037 1592
BlpI GC'TnA_GC
Cuts at: 741 741
Size: 3629
Bme1580I G_kGCm'C
Cuts at: 1254 1347 2403 1254
Size: 93 1056 2480
Fragments arranged by size:
2480 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 435 562 841 922 1257 3401 435
Size: 127 279 81 335 2144 663
Fragments arranged by size:
2144 663 335 279 127 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 341 275
Size: 66 3563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 103 805 1684 2176 2474 103
Size: 702 879 492 298 1258
Fragments arranged by size:
1258 879 702 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3333 3333
Size: 3629
BsaAI yAC'GTr
Cuts at: 982 982
Size: 3629
BsaBI GATnn'nnATC
Cuts at: 6 1143 3180 6
Size: 1137 2037 455
Fragments arranged by size:
2037 1137 455
BsaHI Gr'CG_yC
Cuts at: 479 1060 1308 2010 3506 479
Size: 581 248 702 1496 602
Fragments arranged by size:
1496 702 602 581 248
BsaWI w'CCGG_w
Cuts at: 818 1339 2017 2291 2438 2849 818
Size: 521 678 274 147 411 1598
Fragments arranged by size:
1598 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 217 272 289 292 328 214
Size: 3 55 17 3 36 3515
Fragments arranged by size:
3515 55 36 17 3 3
BseYI C'CCAG_C
Cuts at: 1054 1812 2389 2885 1054
Size: 758 577 496 1798
Fragments arranged by size:
1798 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 532
Size: 3629
BsiEI CG_ry'CG
Cuts at: 104 1171 1217 2425 104
Size: 1067 46 1208 1308
Fragments arranged by size:
1308 1208 1067 46
BsiHKAI G_wGCw'C
Cuts at: 401 607 643 1421 1611 2403 401
Size: 206 36 778 190 792 1627
Fragments arranged by size:
1627 792 778 206 190 36
Bsp1286I G_dGCh'C
Cuts at: 272 401 607 643 1254 1347 1421 1611 1673
Size: 129 206 36 611 93 74 190 62
Cuts at: 1673 2403 272
Size: 730 1498
Fragments arranged by size:
1498 730 611 206 190 129 93 74
62 36
BspEI T'CCGG_A
Cuts at: 2849 2849
Size: 3629
BspHI T'CATG_A
Cuts at: 2805 2805
Size: 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 679 893 1195 1576 679
Size: 214 302 381 2732
Fragments arranged by size:
2732 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1921 1975 3459 3624 50
Size: 1871 54 1484 165 55
Fragments arranged by size:
1871 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 1541
Size: 3629
BsrFI r'CCGG_y
Cuts at: 1627 1808 2017 1627
Size: 181 209 3239
Fragments arranged by size:
3239 209 181
BssSI C'ACGA_G
Cuts at: 214 1900 2258 214
Size: 1686 358 1585
Fragments arranged by size:
1686 1585 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1006 1006
Size: 3629
BstBI TT'CG_AA
Cuts at: 1990 1990
Size: 3629
BstXI CCAn_nnnn'nTGG
Cuts at: 242 242
Size: 3629
BstYI r'GATC_y
Cuts at: 1 654 1121 1144 1479 1725 2737 2823 2835
Size: 653 467 23 335 246 1012 86 12
Cuts at: 2835 3181 1
Size: 346 449
Fragments arranged by size:
1012 653 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1579 1760 1923 1579
Size: 181 163 3285
Fragments arranged by size:
3285 181 163
BtsI GCAGTG_nn'
Cuts at: 872 872
Size: 3629
DraI TTT'AAA
Cuts at: 468 628 468
Size: 160 3469
DrdI GACnn_nn'nnGTC
Cuts at: 1335 2193 1335
Size: 858 2771
EaeI y'GGCC_r
Cuts at: 575 1214 1388 1779 1806 3151 575
Size: 639 174 391 27 1345 1053
Fragments arranged by size:
1345 1053 639 391 174 27
EagI C'GGCC_G
Cuts at: 1214 1214
Size: 3629
EarI CTCTTCn'nnn_
Cuts at: 448 1652 1862 448
Size: 1204 210 2215
Fragments arranged by size:
2215 1204 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1837 2147 2293 3439 1837
Size: 310 146 1146 2027
Fragments arranged by size:
2027 1146 310 146
EcoICRI GAG'CTC
Cuts at: 605 641 605
Size: 36 3593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 341 431 1454 1886 2633 2901 275
Size: 66 90 1023 432 747 268 1003
Fragments arranged by size:
1023 1003 747 432 268 90 66
EcoNI CCTnn'n_nnAGG
Cuts at: 257 257
Size: 3629
EcoO109I rG'GnC_Cy
Cuts at: 768 768
Size: 3629
EcoRI G'AATT_C
Cuts at: 633 633
Size: 3629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1648 1648
Size: 3629
FspI TGC'GCA
Cuts at: 1410 1410
Size: 3629
HaeII r_GCGC'y
Cuts at: 1063 1311 2333 3312 1063
Size: 248 1022 979 1380
Fragments arranged by size:
1380 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 233 242 265 1471 1501 1503 1533 210
Size: 23 9 23 1206 30 2 30 2306
Fragments arranged by size:
2306 1206 30 30 23 23 9 2
HincII GTy'rAC
Cuts at: 441 668 441
Size: 227 3402
HpaI GTT'AAC
Cuts at: 441 441
Size: 3629
KasI G'GCGC_C
Cuts at: 1059 1307 1059
Size: 248 3381
KpnI G_GTAC'C
Cuts at: 649 649
Size: 3629
MluI A'CGCG_T
Cuts at: 3062 3062
Size: 3629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 330 2300 2484 330
Size: 1970 184 1475
Fragments arranged by size:
1970 1475 184
MscI TGG'CCA
Cuts at: 577 1390 577
Size: 813 2816
MslI CAynn'nnrTG
Cuts at: 177 412 487 1618 1745 177
Size: 235 75 1131 127 2061
Fragments arranged by size:
2061 1131 235 127 75
MspA1I CmG'CkG
Cuts at: 740 1054 1414 2427 2672 740
Size: 314 360 1013 245 1697
Fragments arranged by size:
1697 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1810 1810
Size: 3629
NarI GG'CG_CC
Cuts at: 1060 1308 1060
Size: 248 3381
NgoMIV G'CCGG_C
Cuts at: 1808 1808
Size: 3629
NspI r_CATG'y
Cuts at: 176 682 1182 2089 176
Size: 506 500 907 1716
Fragments arranged by size:
1716 907 506 500
PciI A'CATG_T
Cuts at: 2085 2085
Size: 3629
PmeI GTTT'AAAC
Cuts at: 628 628
Size: 3629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 3597
PshAI GACnn'nnGTC
Cuts at: 388 388
Size: 3629
PstI C_TGCA'G
Cuts at: 497 676 1361 497
Size: 179 685 2765
Fragments arranged by size:
2765 685 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3629
PvuII CAG'CTG
Cuts at: 1054 1414 1054
Size: 360 3269
RsrII CG'GwC_CG
Cuts at: 1824 1824
Size: 3629
SacI G_AGCT'C
Cuts at: 607 643 607
Size: 36 3593
SalI G'TCGA_C
Cuts at: 666 666
Size: 3629
SapI GCTCTTCn'nnn_
Cuts at: 1652 1862 1652
Size: 210 3419
SbfI CC_TGCA'GG
Cuts at: 676 676
Size: 3629
ScaI AGT'ACT
Cuts at: 3146 3146
Size: 3629
SfcI C'TryA_G
Cuts at: 33 493 672 1357 2350 2541 33
Size: 460 179 685 993 191 1121
Fragments arranged by size:
1121 993 685 460 191 179
SfoI GGC'GCC
Cuts at: 1061 1309 1061
Size: 248 3381
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
SmaI CCC'GGG
Cuts at: 651 651
Size: 3629
SmlI C'TyrA_G
Cuts at: 118 784 1699 2191 2453 118
Size: 666 915 492 262 1294
Fragments arranged by size:
1294 915 666 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3629
SphI G_CATG'C
Cuts at: 176 682 1182 176
Size: 506 500 2623
Fragments arranged by size:
2623 506 500
StyI C'CwwG_G
Cuts at: 763 1117 763
Size: 354 3275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1212 1345 1804 2013 1212
Size: 133 459 209 2828
Fragments arranged by size:
2828 459 209 133
TatI w'GTAC_w
Cuts at: 3144 3144
Size: 3629
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3597
Tth111I GACn'n_nGTC
Cuts at: 1426 1426
Size: 3629
XbaI T'CTAG_A
Cuts at: 660 660
Size: 3629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1124 1124
Size: 3629
XmaI C'CCGG_G
Cuts at: 649 649
Size: 3629
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI