Restriction map for
pF1KB6836
(Circular) (Six-base) MAPSORT of: pF1KB6836.seq Check: 9214 from: 1 to: 3638
pF1KB6836 3638 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 676 676
Size: 3638
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 3478 3478
Size: 3638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 386 1463 1895 2642 2910 328
Size: 58 1077 432 747 268 1056
Fragments arranged by size:
1077 1056 747 432 268 58
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AleI CACnn'nnGTG
Cuts at: 142 142
Size: 3638
AlwNI CAG_nnn'CTG
Cuts at: 943 2510 943
Size: 1567 2071
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 642 2991 3247 642
Size: 2349 256 1033
Fragments arranged by size:
2349 1033 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 658 658
Size: 3638
BamHI G'GATC_C
Cuts at: 663 663
Size: 3638
BanI G'GyrC_C
Cuts at: 654 1068 1316 1351 654
Size: 414 248 35 2941
Fragments arranged by size:
2941 414 248 35
BanII G_rGCy'C
Cuts at: 115 652 1682 115
Size: 537 1030 2071
Fragments arranged by size:
2071 1030 537
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BciVI GTATCCnnnnn_n'
Cuts at: 1536 2297 3638 1536
Size: 761 1341 1536
Fragments arranged by size:
1536 1341 761
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglII A'GATC_T
Cuts at: 1153 3190 1153
Size: 2037 1601
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 1263 1356 2412 1263
Size: 93 1056 2489
Fragments arranged by size:
2489 1056 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 3638
BmrI ACTGGGnnnn_n'
Cuts at: 850 931 1266 3410 850
Size: 81 335 2144 1078
Fragments arranged by size:
2144 1078 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 517 436
Size: 81 3557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 814 1693 2185 2483 607
Size: 207 879 492 298 1762
Fragments arranged by size:
1762 879 492 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 3342 3342
Size: 3638
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 1152 3189 6
Size: 1146 2037 455
Fragments arranged by size:
2037 1146 455
BsaHI Gr'CG_yC
Cuts at: 392 1069 1317 2019 3515 392
Size: 677 248 702 1496 515
Fragments arranged by size:
1496 702 677 515 248
BsaWI w'CCGG_w
Cuts at: 827 1348 2026 2300 2447 2858 827
Size: 521 678 274 147 411 1607
Fragments arranged by size:
1607 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 292 292
Size: 3638
BseYI C'CCAG_C
Cuts at: 570 586 591 1063 1821 2398 2894 570
Size: 16 5 472 758 577 496 1314
Fragments arranged by size:
1314 758 577 496 472 16 5
BsiEI CG_ry'CG
Cuts at: 104 225 333 1180 1226 2434 104
Size: 121 108 847 46 1208 1308
Fragments arranged by size:
1308 1208 847 121 108 46
BsiHKAI G_wGCw'C
Cuts at: 115 652 1430 1620 2412 115
Size: 537 778 190 792 1341
Fragments arranged by size:
1341 792 778 537 190
BsmBI CGTCTCn'nnnn_
Cuts at: 307 307
Size: 3638
Bsp1286I G_dGCh'C
Cuts at: 115 652 1263 1356 1430 1620 1682 2412 115
Size: 537 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 730 611 537 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2858 2858
Size: 3638
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 188 688 902 1204 1585 188
Size: 500 214 302 381 2241
Fragments arranged by size:
2241 500 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1930 1984 3468 3633 50
Size: 1880 54 1484 165 55
Fragments arranged by size:
1880 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 1636 1817 2026 1636
Size: 181 209 3248
Fragments arranged by size:
3248 209 181
BssSI C'ACGA_G
Cuts at: 1909 2267 1909
Size: 358 3280
BstAPI GCAn_nnn'nTGC
Cuts at: 1015 1015
Size: 3638
BstBI TT'CG_AA
Cuts at: 608 1999 608
Size: 1391 2247
BstEII G'GTnAC_C
Cuts at: 544 544
Size: 3638
BstXI CCAn_nnnn'nTGG
Cuts at: 144 414 144
Size: 270 3368
BstYI r'GATC_y
Cuts at: 1 444 663 1130 1153 1488 1734 2746 2832
Size: 443 219 467 23 335 246 1012 86
Cuts at: 2832 2844 3190 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 443 346 335 246 219
86 23 12
BtgI C'CryG_G
Cuts at: 407 407
Size: 3638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 242 1588 1769 1932 242
Size: 1346 181 163 1948
Fragments arranged by size:
1948 1346 181 163
BtsI GCAGTG_nn'
Cuts at: 881 881
Size: 3638
DraI TTT'AAA
Cuts at: 637 637
Size: 3638
DrdI GACnn_nn'nnGTC
Cuts at: 1344 2202 1344
Size: 858 2780
EaeI y'GGCC_r
Cuts at: 272 330 1223 1397 1788 1815 3160 272
Size: 58 893 174 391 27 1345 750
Fragments arranged by size:
1345 893 750 391 174 58 27
EagI C'GGCC_G
Cuts at: 330 1223 330
Size: 893 2745
EarI CTCTTCn'nnn_
Cuts at: 346 610 1661 1871 346
Size: 264 1051 210 2113
Fragments arranged by size:
2113 1051 264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1846 2156 2302 3448 1846
Size: 310 146 1146 2036
Fragments arranged by size:
2036 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 650 113
Size: 537 3101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 386 436 517 1463 1895 2642 2910 328
Size: 58 50 81 946 432 747 268 1056
Fragments arranged by size:
1056 946 747 432 268 81 58 50
EcoNI CCTnn'n_nnAGG
Cuts at: 514 514
Size: 3638
EcoO109I rG'GnC_Cy
Cuts at: 435 777 435
Size: 342 3296
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 544 1657 544
Size: 1113 2525
FspI TGC'GCA
Cuts at: 1419 1419
Size: 3638
HaeII r_GCGC'y
Cuts at: 1072 1320 2342 3321 1072
Size: 248 1022 979 1389
Fragments arranged by size:
1389 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1480 1510 1512 1542 1480
Size: 30 2 30 3576
Fragments arranged by size:
3576 30 30 2
HincII GTy'rAC
Cuts at: 159 677 159
Size: 518 3120
HindIII A'AGCT_T
Cuts at: 526 526
Size: 3638
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2493 2309
Size: 184 3454
MscI TGG'CCA
Cuts at: 1399 1399
Size: 3638
MslI CAynn'nnrTG
Cuts at: 142 485 1627 1754 142
Size: 343 1142 127 2026
Fragments arranged by size:
2026 1142 343 127
MspA1I CmG'CkG
Cuts at: 364 749 1063 1423 2436 2681 364
Size: 385 314 360 1013 245 1321
Fragments arranged by size:
1321 1013 385 360 314 245
NaeI GCC'GGC
Cuts at: 1819 1819
Size: 3638
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NcoI C'CATG_G
Cuts at: 407 407
Size: 3638
NgoMIV G'CCGG_C
Cuts at: 1817 1817
Size: 3638
NotI GC'GGCC_GC
Cuts at: 330 330
Size: 3638
NspI r_CATG'y
Cuts at: 691 1191 2098 691
Size: 500 907 2231
Fragments arranged by size:
2231 907 500
PasI CC'CwG_GG
Cuts at: 456 456
Size: 3638
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PflMI CCAn_nnn'nTGG
Cuts at: 570 570
Size: 3638
PfoI T'CCnGG_A
Cuts at: 344 494 344
Size: 150 3488
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 3606
PstI C_TGCA'G
Cuts at: 185 685 1370 185
Size: 500 685 2453
Fragments arranged by size:
2453 685 500
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 364 1063 1423 364
Size: 699 360 2579
Fragments arranged by size:
2579 699 360
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 115 652 115
Size: 537 3101
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 1661 1871 1661
Size: 210 3428
SbfI CC_TGCA'GG
Cuts at: 185 685 185
Size: 500 3138
ScaI AGT'ACT
Cuts at: 3155 3155
Size: 3638
SfcI C'TryA_G
Cuts at: 33 181 681 1366 2359 2550 33
Size: 148 500 685 993 191 1121
Fragments arranged by size:
1121 993 685 500 191 148
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 660 660
Size: 3638
SmlI C'TyrA_G
Cuts at: 622 793 1708 2200 2462 622
Size: 171 915 492 262 1798
Fragments arranged by size:
1798 915 492 262 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
StuI AGG'CCT
Cuts at: 187 187
Size: 3638
StyI C'CwwG_G
Cuts at: 407 772 1126 407
Size: 365 354 2919
Fragments arranged by size:
2919 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 532 562 1221 1354 1813 2022 532
Size: 30 659 133 459 209 2148
Fragments arranged by size:
2148 659 459 209 133 30
TatI w'GTAC_w
Cuts at: 3153 3153
Size: 3638
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3606
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 669 669
Size: 3638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 571 1133 571
Size: 562 3076
XmaI C'CCGG_G
Cuts at: 658 658
Size: 3638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NruI NsiI PacI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI