Restriction map for
pF1KB6806
(Circular) (Six-base) MAPSORT of: pF1KB6806.seq Check: 154 from: 1 to: 3665
pF1KB6806 3665 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 703 703
Size: 3665
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 1922 2669 2937 1490
Size: 432 747 268 2218
Fragments arranged by size:
2218 747 432 268
AflIII A'CryG_T
Cuts at: 1017 2121 3098 1017
Size: 1104 977 1584
Fragments arranged by size:
1584 1104 977
AgeI A'CCGG_T
Cuts at: 2053 2053
Size: 3665
AlwNI CAG_nnn'CTG
Cuts at: 622 970 2537 622
Size: 348 1567 1750
Fragments arranged by size:
1750 1567 348
ApaI G_GGCC'C
Cuts at: 285 285
Size: 3665
ApaLI G'TGCA_C
Cuts at: 2435 2435
Size: 3665
ApoI r'AATT_y
Cuts at: 669 3018 3274 669
Size: 2349 256 1060
Fragments arranged by size:
2349 1060 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3665
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 685 685
Size: 3665
BamHI G'GATC_C
Cuts at: 205 690 205
Size: 485 3180
BanI G'GyrC_C
Cuts at: 124 681 1095 1343 1378 124
Size: 557 414 248 35 2411
Fragments arranged by size:
2411 557 414 248 35
BanII G_rGCy'C
Cuts at: 285 679 1709 285
Size: 394 1030 2241
Fragments arranged by size:
2241 1030 394
BbeI G_GCGC'C
Cuts at: 128 1099 1347 128
Size: 971 248 2446
Fragments arranged by size:
2446 971 248
BbvCI CC'TCA_GC
Cuts at: 220 220
Size: 3665
BciVI GTATCCnnnnn_n'
Cuts at: 1563 2324 3665 1563
Size: 761 1341 1563
Fragments arranged by size:
1563 1341 761
BclI T'GATC_A
Cuts at: 1185 1185
Size: 3665
BglI GCCn_nnn'nGGC
Cuts at: 170 170
Size: 3665
BglII A'GATC_T
Cuts at: 1180 3217 1180
Size: 2037 1628
BlpI GC'TnA_GC
Cuts at: 265 777 265
Size: 512 3153
Bme1580I G_kGCm'C
Cuts at: 285 1290 1383 2439 285
Size: 1005 93 1056 1511
Fragments arranged by size:
1511 1056 1005 93
BmrI ACTGGGnnnn_n'
Cuts at: 877 958 1293 3437 877
Size: 81 335 2144 1105
Fragments arranged by size:
2144 1105 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 3665
Bpu10I CC'TnA_GC
Cuts at: 220 220
Size: 3665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1720 2212 2510 841
Size: 879 492 298 1996
Fragments arranged by size:
1996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3369 3369
Size: 3665
BsaAI yAC'GTr
Cuts at: 1018 1018
Size: 3665
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 125 1096 1344 2046 3542 125
Size: 971 248 702 1496 248
Fragments arranged by size:
1496 971 702 248 248
BsaWI w'CCGG_w
Cuts at: 854 1375 2053 2327 2474 2885 854
Size: 521 678 274 147 411 1634
Fragments arranged by size:
1634 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 520
Size: 3665
BseYI C'CCAG_C
Cuts at: 118 190 1090 1848 2425 2921 118
Size: 72 900 758 577 496 862
Fragments arranged by size:
900 862 758 577 496 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 166
Size: 3665
BsiEI CG_ry'CG
Cuts at: 104 170 174 262 1207 1253 2461 104
Size: 66 4 88 945 46 1208 1308
Fragments arranged by size:
1308 1208 945 88 66 46 4
BsiHKAI G_wGCw'C
Cuts at: 679 1457 1647 2439 679
Size: 778 190 792 1905
Fragments arranged by size:
1905 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 285 679 1290 1383 1457 1647 1709 2439 285
Size: 394 611 93 74 190 62 730 1511
Fragments arranged by size:
1511 730 611 394 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2885 2885
Size: 3665
BspHI T'CATG_A
Cuts at: 2841 2841
Size: 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 715 929 1231 1612 715
Size: 214 302 381 2768
Fragments arranged by size:
2768 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1957 2011 3495 3660 50
Size: 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1577 1577
Size: 3665
BsrFI r'CCGG_y
Cuts at: 169 173 257 1663 1844 2053 169
Size: 4 84 1406 181 209 1781
Fragments arranged by size:
1781 1406 209 181 84 4
BssHII G'CGCG_C
Cuts at: 138 138
Size: 3665
BssSI C'ACGA_G
Cuts at: 1936 2294 1936
Size: 358 3307
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 1042
Size: 3665
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstXI CCAn_nnnn'nTGG
Cuts at: 645 645
Size: 3665
BstYI r'GATC_y
Cuts at: 1 205 690 1157 1180 1515 1761 2773 2859
Size: 204 485 467 23 335 246 1012 86
Cuts at: 2859 2871 3217 1
Size: 12 346 449
Fragments arranged by size:
1012 485 467 449 346 335 246 204
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3665
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1615 1796 1959 1615
Size: 181 163 3321
Fragments arranged by size:
3321 181 163
BtsI GCAGTG_nn'
Cuts at: 908 908
Size: 3665
DraI TTT'AAA
Cuts at: 664 664
Size: 3665
DrdI GACnn_nn'nnGTC
Cuts at: 1371 2229 1371
Size: 858 2807
EaeI y'GGCC_r
Cuts at: 167 171 1250 1424 1815 1842 3187 167
Size: 4 1079 174 391 27 1345 645
Fragments arranged by size:
1345 1079 645 391 174 27 4
EagI C'GGCC_G
Cuts at: 167 171 1250 167
Size: 4 1079 2582
Fragments arranged by size:
2582 1079 4
EarI CTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1873 2183 2329 3475 1873
Size: 310 146 1146 2063
Fragments arranged by size:
2063 1146 310 146
EcoICRI GAG'CTC
Cuts at: 677 677
Size: 3665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 1922 2669 2937 1490
Size: 432 747 268 2218
Fragments arranged by size:
2218 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 194 804 194
Size: 610 3055
EcoRI G'AATT_C
Cuts at: 669 669
Size: 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3665
FseI GG_CCGG'CC
Cuts at: 173 173
Size: 3665
FspI TGC'GCA
Cuts at: 1446 1446
Size: 3665
HaeII r_GCGC'y
Cuts at: 128 1099 1347 2369 3348 128
Size: 971 248 1022 979 445
Fragments arranged by size:
1022 979 971 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1507 1537 1539 1569 1507
Size: 30 2 30 3603
Fragments arranged by size:
3603 30 30 2
HincII GTy'rAC
Cuts at: 704 704
Size: 3665
KasI G'GCGC_C
Cuts at: 124 1095 1343 124
Size: 971 248 2446
Fragments arranged by size:
2446 971 248
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MluI A'CGCG_T
Cuts at: 3098 3098
Size: 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2520 2336
Size: 184 3481
MscI TGG'CCA
Cuts at: 1426 1426
Size: 3665
MslI CAynn'nnrTG
Cuts at: 476 643 1654 1781 476
Size: 167 1011 127 2360
Fragments arranged by size:
2360 1011 167 127
MspA1I CmG'CkG
Cuts at: 118 212 776 1090 1450 2463 2708 118
Size: 94 564 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 564 360 314 245 94
NaeI GCC'GGC
Cuts at: 171 1846 171
Size: 1675 1990
NarI GG'CG_CC
Cuts at: 125 1096 1344 125
Size: 971 248 2446
Fragments arranged by size:
2446 971 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3665
NdeI CA'TA_TG
Cuts at: 531 531
Size: 3665
NgoMIV G'CCGG_C
Cuts at: 169 1844 169
Size: 1675 1990
NspI r_CATG'y
Cuts at: 425 519 718 1218 2125 425
Size: 94 199 500 907 1965
Fragments arranged by size:
1965 907 500 199 94
PciI A'CATG_T
Cuts at: 2121 2121
Size: 3665
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 3633
PpuMI rG'GwC_Cy
Cuts at: 194 194
Size: 3665
PsiI TTA'TAA
Cuts at: 322 322
Size: 3665
PspOMI G'GGCC_C
Cuts at: 281 281
Size: 3665
PstI C_TGCA'G
Cuts at: 712 1397 712
Size: 685 2980
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 1090 1450 1090
Size: 360 3305
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 679 679
Size: 3665
SalI G'TCGA_C
Cuts at: 702 702
Size: 3665
SanDI GG'GwC_CC
Cuts at: 194 194
Size: 3665
SapI GCTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SfcI C'TryA_G
Cuts at: 33 708 1393 2386 2577 33
Size: 675 685 993 191 1121
Fragments arranged by size:
1121 993 685 675 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 170 170
Size: 3665
SfoI GGC'GCC
Cuts at: 126 1097 1345 126
Size: 971 248 2446
Fragments arranged by size:
2446 971 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 820 1735 2227 2489 820
Size: 915 492 262 1996
Fragments arranged by size:
1996 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 718 1218 718
Size: 500 3165
StyI C'CwwG_G
Cuts at: 107 799 1153 107
Size: 692 354 2619
Fragments arranged by size:
2619 692 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1248 1381 1840 2049 1248
Size: 133 459 209 2864
Fragments arranged by size:
2864 459 209 133
TatI w'GTAC_w
Cuts at: 3180 3180
Size: 3665
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 178 210 3182 3214 178
Size: 32 2972 32 629
Fragments arranged by size:
2972 629 32 32
Tth111I GACn'n_nGTC
Cuts at: 1462 1462
Size: 3665
XbaI T'CTAG_A
Cuts at: 399 696 399
Size: 297 3368
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1160 1160
Size: 3665
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PspXI PsrI SacII SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI