Restriction map for
pF1KB6796
(Circular) (Six-base) MAPSORT of: pF1KB6796.seq Check: 8399 from: 1 to: 3581
pF1KB6796 3581 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 301 619 301
Size: 318 3263
Acc65I G'GTAC_C
Cuts at: 597 597
Size: 3581
AclI AA'CG_TT
Cuts at: 3421 3421
Size: 3581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1406 1838 2585 2853 1406
Size: 432 747 268 2134
Fragments arranged by size:
2134 747 432 268
AflIII A'CryG_T
Cuts at: 933 2037 3014 933
Size: 1104 977 1500
Fragments arranged by size:
1500 1104 977
AgeI A'CCGG_T
Cuts at: 1969 1969
Size: 3581
AlwNI CAG_nnn'CTG
Cuts at: 234 886 2453 234
Size: 652 1567 1362
Fragments arranged by size:
1567 1362 652
ApaLI G'TGCA_C
Cuts at: 2351 2351
Size: 3581
ApoI r'AATT_y
Cuts at: 212 585 2934 3190 212
Size: 373 2349 256 603
Fragments arranged by size:
2349 603 373 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
AvaI C'yCGr_G
Cuts at: 151 601 151
Size: 450 3131
BamHI G'GATC_C
Cuts at: 606 606
Size: 3581
BanI G'GyrC_C
Cuts at: 597 1011 1259 1294 597
Size: 414 248 35 2884
Fragments arranged by size:
2884 414 248 35
BanII G_rGCy'C
Cuts at: 595 1625 595
Size: 1030 2551
BbeI G_GCGC'C
Cuts at: 1015 1263 1015
Size: 248 3333
BbsI GAAGACnn'nnnn_
Cuts at: 145 575 145
Size: 430 3151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3547
BciVI GTATCCnnnnn_n'
Cuts at: 162 1479 2240 3581 162
Size: 1317 761 1341 162
Fragments arranged by size:
1341 1317 761 162
BclI T'GATC_A
Cuts at: 222 1101 222
Size: 879 2702
BglII A'GATC_T
Cuts at: 1096 3133 1096
Size: 2037 1544
BlpI GC'TnA_GC
Cuts at: 693 693
Size: 3581
Bme1580I G_kGCm'C
Cuts at: 472 1206 1299 2355 472
Size: 734 93 1056 1698
Fragments arranged by size:
1698 1056 734 93
BmrI ACTGGGnnnn_n'
Cuts at: 793 874 1209 3353 793
Size: 81 335 2144 1021
Fragments arranged by size:
2144 1021 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 1636 2128 2426 757
Size: 879 492 298 1912
Fragments arranged by size:
1912 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3285 3285
Size: 3581
BsaAI yAC'GTr
Cuts at: 934 934
Size: 3581
BsaBI GATnn'nnATC
Cuts at: 6 1095 3132 6
Size: 1089 2037 455
Fragments arranged by size:
2037 1089 455
BsaHI Gr'CG_yC
Cuts at: 1012 1260 1962 3458 1012
Size: 248 702 1496 1135
Fragments arranged by size:
1496 1135 702 248
BsaWI w'CCGG_w
Cuts at: 770 1291 1969 2243 2390 2801 770
Size: 521 678 274 147 411 1550
Fragments arranged by size:
1550 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 490 1006 1764 2341 2837 490
Size: 516 758 577 496 1234
Fragments arranged by size:
1234 758 577 516 496
BsiEI CG_ry'CG
Cuts at: 104 1123 1169 2377 104
Size: 1019 46 1208 1308
Fragments arranged by size:
1308 1208 1019 46
BsiHKAI G_wGCw'C
Cuts at: 595 1373 1563 2355 595
Size: 778 190 792 1821
Fragments arranged by size:
1821 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 472 595 1206 1299 1373 1563 1625 2355 472
Size: 123 611 93 74 190 62 730 1698
Fragments arranged by size:
1698 730 611 190 123 93 74 62
BspEI T'CCGG_A
Cuts at: 2801 2801
Size: 3581
BspHI T'CATG_A
Cuts at: 2757 2757
Size: 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 631 845 1147 1528 631
Size: 214 302 381 2684
Fragments arranged by size:
2684 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1873 1927 3411 3576 50
Size: 1823 54 1484 165 55
Fragments arranged by size:
1823 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 1493
Size: 3581
BsrFI r'CCGG_y
Cuts at: 1579 1760 1969 1579
Size: 181 209 3191
Fragments arranged by size:
3191 209 181
BssSI C'ACGA_G
Cuts at: 1852 2210 1852
Size: 358 3223
BstAPI GCAn_nnn'nTGC
Cuts at: 958 958
Size: 3581
BstBI TT'CG_AA
Cuts at: 1942 1942
Size: 3581
BstYI r'GATC_y
Cuts at: 1 194 606 1073 1096 1431 1677 2689 2775
Size: 193 412 467 23 335 246 1012 86
Cuts at: 2775 2787 3133 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 412 346 335 246 193
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1531 1712 1875 1531
Size: 181 163 3237
Fragments arranged by size:
3237 181 163
BtsI GCAGTG_nn'
Cuts at: 824 824
Size: 3581
DraI TTT'AAA
Cuts at: 580 580
Size: 3581
DrdI GACnn_nn'nnGTC
Cuts at: 1287 2145 1287
Size: 858 2723
EaeI y'GGCC_r
Cuts at: 1166 1340 1731 1758 3103 1166
Size: 174 391 27 1345 1644
Fragments arranged by size:
1644 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1166 1166
Size: 3581
EarI CTCTTCn'nnn_
Cuts at: 311 1604 1814 311
Size: 1293 210 2078
Fragments arranged by size:
2078 1293 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1789 2099 2245 3391 1789
Size: 310 146 1146 1979
Fragments arranged by size:
1979 1146 310 146
EcoICRI GAG'CTC
Cuts at: 593 593
Size: 3581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1406 1838 2585 2853 1406
Size: 432 747 268 2134
Fragments arranged by size:
2134 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 720 720
Size: 3581
EcoRI G'AATT_C
Cuts at: 212 585 212
Size: 373 3208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1600 1600
Size: 3581
FspI TGC'GCA
Cuts at: 1362 1362
Size: 3581
HaeII r_GCGC'y
Cuts at: 1015 1263 2285 3264 1015
Size: 248 1022 979 1332
Fragments arranged by size:
1332 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1423 1453 1455 1485 1423
Size: 30 2 30 3519
Fragments arranged by size:
3519 30 30 2
HincII GTy'rAC
Cuts at: 620 620
Size: 3581
KasI G'GCGC_C
Cuts at: 1011 1259 1011
Size: 248 3333
KpnI G_GTAC'C
Cuts at: 601 601
Size: 3581
MluI A'CGCG_T
Cuts at: 3014 3014
Size: 3581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2252 2436 2252
Size: 184 3397
MscI TGG'CCA
Cuts at: 1342 1342
Size: 3581
MslI CAynn'nnrTG
Cuts at: 527 1570 1697 527
Size: 1043 127 2411
Fragments arranged by size:
2411 1043 127
MspA1I CmG'CkG
Cuts at: 255 692 1006 1366 2379 2624 255
Size: 437 314 360 1013 245 1212
Fragments arranged by size:
1212 1013 437 360 314 245
NaeI GCC'GGC
Cuts at: 1762 1762
Size: 3581
NarI GG'CG_CC
Cuts at: 1012 1260 1012
Size: 248 3333
NgoMIV G'CCGG_C
Cuts at: 1760 1760
Size: 3581
NspI r_CATG'y
Cuts at: 634 1134 2041 634
Size: 500 907 2174
Fragments arranged by size:
2174 907 500
PciI A'CATG_T
Cuts at: 2037 2037
Size: 3581
PfoI T'CCnGG_A
Cuts at: 197 197
Size: 3581
PmeI GTTT'AAAC
Cuts at: 580 580
Size: 3581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2720 2752 2720
Size: 32 3549
PsiI TTA'TAA
Cuts at: 425 425
Size: 3581
PspXI vC'TCGA_Gb
Cuts at: 151 151
Size: 3581
PstI C_TGCA'G
Cuts at: 628 1313 628
Size: 685 2896
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3581
PvuII CAG'CTG
Cuts at: 255 1006 1366 255
Size: 751 360 2470
Fragments arranged by size:
2470 751 360
RsrII CG'GwC_CG
Cuts at: 1776 1776
Size: 3581
SacI G_AGCT'C
Cuts at: 595 595
Size: 3581
SalI G'TCGA_C
Cuts at: 618 618
Size: 3581
SapI GCTCTTCn'nnn_
Cuts at: 1604 1814 1604
Size: 210 3371
SbfI CC_TGCA'GG
Cuts at: 628 628
Size: 3581
ScaI AGT'ACT
Cuts at: 3098 3098
Size: 3581
SfcI C'TryA_G
Cuts at: 33 624 1309 2302 2493 33
Size: 591 685 993 191 1121
Fragments arranged by size:
1121 993 685 591 191
SfoI GGC'GCC
Cuts at: 1013 1261 1013
Size: 248 3333
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
SmaI CCC'GGG
Cuts at: 603 603
Size: 3581
SmlI C'TyrA_G
Cuts at: 151 736 1651 2143 2405 151
Size: 585 915 492 262 1327
Fragments arranged by size:
1327 915 585 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3581
SphI G_CATG'C
Cuts at: 634 1134 634
Size: 500 3081
SspI AAT'ATT
Cuts at: 288 421 288
Size: 133 3448
StyI C'CwwG_G
Cuts at: 715 1069 715
Size: 354 3227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1164 1297 1756 1965 1164
Size: 133 459 209 2780
Fragments arranged by size:
2780 459 209 133
TatI w'GTAC_w
Cuts at: 3096 3096
Size: 3581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3549
Tth111I GACn'n_nGTC
Cuts at: 1378 1378
Size: 3581
XbaI T'CTAG_A
Cuts at: 612 612
Size: 3581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1076 1076
Size: 3581
XhoI C'TCGA_G
Cuts at: 151 151
Size: 3581
XmaI C'CCGG_G
Cuts at: 601 601
Size: 3581
XmnI GAAnn'nnTTC
Cuts at: 212 212
Size: 3581
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PsiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI ZraI