Restriction map for
pF1KB6792
(Circular) (Six-base) MAPSORT of: pF1KB6792.seq Check: 355 from: 1 to: 3578
pF1KB6792 3578 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 616 616
Size: 3578
Acc65I G'GTAC_C
Cuts at: 594 594
Size: 3578
AclI AA'CG_TT
Cuts at: 3418 3418
Size: 3578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 1403 1835 2582 2850 503
Size: 900 432 747 268 1231
Fragments arranged by size:
1231 900 747 432 268
AflIII A'CryG_T
Cuts at: 930 2034 3011 930
Size: 1104 977 1497
Fragments arranged by size:
1497 1104 977
AgeI A'CCGG_T
Cuts at: 1966 1966
Size: 3578
AlwNI CAG_nnn'CTG
Cuts at: 309 883 2450 309
Size: 574 1567 1437
Fragments arranged by size:
1567 1437 574
ApaLI G'TGCA_C
Cuts at: 471 2348 471
Size: 1877 1701
ApoI r'AATT_y
Cuts at: 582 2931 3187 582
Size: 2349 256 973
Fragments arranged by size:
2349 973 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3578
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
AvaI C'yCGr_G
Cuts at: 456 517 598 456
Size: 61 81 3436
Fragments arranged by size:
3436 81 61
BamHI G'GATC_C
Cuts at: 603 603
Size: 3578
BanI G'GyrC_C
Cuts at: 379 477 594 1008 1256 1291 379
Size: 98 117 414 248 35 2666
Fragments arranged by size:
2666 414 248 117 98 35
BanII G_rGCy'C
Cuts at: 371 428 592 1622 371
Size: 57 164 1030 2327
Fragments arranged by size:
2327 1030 164 57
BbeI G_GCGC'C
Cuts at: 1012 1260 1012
Size: 248 3330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 430 464 430
Size: 34 3544
BciVI GTATCCnnnnn_n'
Cuts at: 1476 2237 3578 1476
Size: 761 1341 1476
Fragments arranged by size:
1476 1341 761
BclI T'GATC_A
Cuts at: 1098 1098
Size: 3578
BglII A'GATC_T
Cuts at: 1093 3130 1093
Size: 2037 1541
BlpI GC'TnA_GC
Cuts at: 690 690
Size: 3578
Bme1580I G_kGCm'C
Cuts at: 475 1203 1296 2352 475
Size: 728 93 1056 1701
Fragments arranged by size:
1701 1056 728 93
BmrI ACTGGGnnnn_n'
Cuts at: 790 871 1206 3350 790
Size: 81 335 2144 1018
Fragments arranged by size:
2144 1018 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1945 1945
Size: 3578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 417 212
Size: 205 3373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1633 2125 2423 754
Size: 879 492 298 1909
Fragments arranged by size:
1909 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3282 3282
Size: 3578
BsaAI yAC'GTr
Cuts at: 931 931
Size: 3578
BsaBI GATnn'nnATC
Cuts at: 6 1092 3129 6
Size: 1086 2037 455
Fragments arranged by size:
2037 1086 455
BsaHI Gr'CG_yC
Cuts at: 1009 1257 1959 3455 1009
Size: 248 702 1496 1132
Fragments arranged by size:
1496 1132 702 248
BsaWI w'CCGG_w
Cuts at: 767 1288 1966 2240 2387 2798 767
Size: 521 678 274 147 411 1547
Fragments arranged by size:
1547 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 509 1003 1761 2338 2834 509
Size: 494 758 577 496 1253
Fragments arranged by size:
1253 758 577 496 494
BsiEI CG_ry'CG
Cuts at: 104 1120 1166 2374 104
Size: 1016 46 1208 1308
Fragments arranged by size:
1308 1208 1016 46
BsiHKAI G_wGCw'C
Cuts at: 371 475 592 1370 1560 2352 371
Size: 104 117 778 190 792 1597
Fragments arranged by size:
1597 792 778 190 117 104
BsmI GAATG_Cn'
Cuts at: 540 540
Size: 3578
Bsp1286I G_dGCh'C
Cuts at: 371 428 475 592 1203 1296 1370 1560 1622
Size: 57 47 117 611 93 74 190 62
Cuts at: 1622 2352 371
Size: 730 1597
Fragments arranged by size:
1597 730 611 190 117 93 74 62
57 47
BspEI T'CCGG_A
Cuts at: 2798 2798
Size: 3578
BspHI T'CATG_A
Cuts at: 2754 2754
Size: 3578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 628 842 1144 1525 628
Size: 214 302 381 2681
Fragments arranged by size:
2681 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1870 1924 3408 3573 50
Size: 1820 54 1484 165 55
Fragments arranged by size:
1820 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1490 1490
Size: 3578
BsrFI r'CCGG_y
Cuts at: 410 1576 1757 1966 410
Size: 1166 181 209 2022
Fragments arranged by size:
2022 1166 209 181
BssSI C'ACGA_G
Cuts at: 1849 2207 1849
Size: 358 3220
BstAPI GCAn_nnn'nTGC
Cuts at: 955 955
Size: 3578
BstBI TT'CG_AA
Cuts at: 1939 1939
Size: 3578
BstYI r'GATC_y
Cuts at: 1 603 1070 1093 1428 1674 2686 2772 2784
Size: 602 467 23 335 246 1012 86 12
Cuts at: 2784 3130 1
Size: 346 449
Fragments arranged by size:
1012 602 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1528 1709 1872 1528
Size: 181 163 3234
Fragments arranged by size:
3234 181 163
BtsI GCAGTG_nn'
Cuts at: 571 821 571
Size: 250 3328
DraI TTT'AAA
Cuts at: 577 577
Size: 3578
DrdI GACnn_nn'nnGTC
Cuts at: 1284 2142 1284
Size: 858 2720
EaeI y'GGCC_r
Cuts at: 1163 1337 1728 1755 3100 1163
Size: 174 391 27 1345 1641
Fragments arranged by size:
1641 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1163 1163
Size: 3578
EarI CTCTTCn'nnn_
Cuts at: 221 1601 1811 221
Size: 1380 210 1988
Fragments arranged by size:
1988 1380 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1786 2096 2242 3388 1786
Size: 310 146 1146 1976
Fragments arranged by size:
1976 1146 310 146
EcoICRI GAG'CTC
Cuts at: 369 590 369
Size: 221 3357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 417 503 1403 1835 2582 2850 212
Size: 205 86 900 432 747 268 940
Fragments arranged by size:
940 900 747 432 268 205 86
EcoO109I rG'GnC_Cy
Cuts at: 717 717
Size: 3578
EcoRI G'AATT_C
Cuts at: 582 582
Size: 3578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1597 1597
Size: 3578
FspI TGC'GCA
Cuts at: 1359 1359
Size: 3578
HaeII r_GCGC'y
Cuts at: 127 1012 1260 2282 3261 127
Size: 885 248 1022 979 444
Fragments arranged by size:
1022 979 885 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1420 1450 1452 1482 1420
Size: 30 2 30 3516
Fragments arranged by size:
3516 30 30 2
HincII GTy'rAC
Cuts at: 617 617
Size: 3578
KasI G'GCGC_C
Cuts at: 1008 1256 1008
Size: 248 3330
KpnI G_GTAC'C
Cuts at: 598 598
Size: 3578
MluI A'CGCG_T
Cuts at: 3011 3011
Size: 3578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2249 2433 2249
Size: 184 3394
MscI TGG'CCA
Cuts at: 1339 1339
Size: 3578
MslI CAynn'nnrTG
Cuts at: 1567 1694 1567
Size: 127 3451
MspA1I CmG'CkG
Cuts at: 689 1003 1363 2376 2621 689
Size: 314 360 1013 245 1646
Fragments arranged by size:
1646 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1759 1759
Size: 3578
NarI GG'CG_CC
Cuts at: 1009 1257 1009
Size: 248 3330
NcoI C'CATG_G
Cuts at: 107 107
Size: 3578
NgoMIV G'CCGG_C
Cuts at: 1757 1757
Size: 3578
NspI r_CATG'y
Cuts at: 205 631 1131 2038 205
Size: 426 500 907 1745
Fragments arranged by size:
1745 907 500 426
PciI A'CATG_T
Cuts at: 2034 2034
Size: 3578
PflMI CCAn_nnn'nTGG
Cuts at: 251 251
Size: 3578
PmeI GTTT'AAAC
Cuts at: 577 577
Size: 3578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2717 2749 2717
Size: 32 3546
PstI C_TGCA'G
Cuts at: 625 1310 625
Size: 685 2893
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3578
PvuII CAG'CTG
Cuts at: 1003 1363 1003
Size: 360 3218
RsrII CG'GwC_CG
Cuts at: 1773 1773
Size: 3578
SacI G_AGCT'C
Cuts at: 371 592 371
Size: 221 3357
SalI G'TCGA_C
Cuts at: 615 615
Size: 3578
SapI GCTCTTCn'nnn_
Cuts at: 1601 1811 1601
Size: 210 3368
SbfI CC_TGCA'GG
Cuts at: 625 625
Size: 3578
ScaI AGT'ACT
Cuts at: 3095 3095
Size: 3578
SfcI C'TryA_G
Cuts at: 33 621 1306 2299 2490 33
Size: 588 685 993 191 1121
Fragments arranged by size:
1121 993 685 588 191
SfoI GGC'GCC
Cuts at: 1010 1258 1010
Size: 248 3330
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3578
SmaI CCC'GGG
Cuts at: 458 600 458
Size: 142 3436
SmlI C'TyrA_G
Cuts at: 733 1648 2140 2402 733
Size: 915 492 262 1909
Fragments arranged by size:
1909 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3578
SphI G_CATG'C
Cuts at: 205 631 1131 205
Size: 426 500 2652
Fragments arranged by size:
2652 500 426
StyI C'CwwG_G
Cuts at: 107 383 712 1066 107
Size: 276 329 354 2619
Fragments arranged by size:
2619 354 329 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 523 1161 1294 1753 1962 274
Size: 249 638 133 459 209 1890
Fragments arranged by size:
1890 638 459 249 209 133
TatI w'GTAC_w
Cuts at: 3093 3093
Size: 3578
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 498 530 3095 3127 498
Size: 32 2565 32 949
Fragments arranged by size:
2565 949 32 32
Tth111I GACn'n_nGTC
Cuts at: 1375 1375
Size: 3578
XbaI T'CTAG_A
Cuts at: 609 609
Size: 3578
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 187 252 1073 187
Size: 65 821 2692
Fragments arranged by size:
2692 821 65
XmaI C'CCGG_G
Cuts at: 456 598 456
Size: 142 3436
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI