Restriction map for
pF1KB6787
(Circular) (Six-base) MAPSORT of: pF1KB6787.seq Check: 2415 from: 1 to: 3488
pF1KB6787 3488 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 526 526
Size: 3488
Acc65I G'GTAC_C
Cuts at: 504 504
Size: 3488
AclI AA'CG_TT
Cuts at: 3328 3328
Size: 3488
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 358 1313 1745 2492 2760 355
Size: 3 955 432 747 268 1083
Fragments arranged by size:
1083 955 747 432 268 3
AflIII A'CryG_T
Cuts at: 840 1944 2921 840
Size: 1104 977 1407
Fragments arranged by size:
1407 1104 977
AgeI A'CCGG_T
Cuts at: 1876 1876
Size: 3488
AlwNI CAG_nnn'CTG
Cuts at: 469 793 2360 469
Size: 324 1567 1597
Fragments arranged by size:
1597 1567 324
ApaLI G'TGCA_C
Cuts at: 2258 2258
Size: 3488
ApoI r'AATT_y
Cuts at: 492 2841 3097 492
Size: 2349 256 883
Fragments arranged by size:
2349 883 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3488
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
AvaI C'yCGr_G
Cuts at: 508 508
Size: 3488
BamHI G'GATC_C
Cuts at: 513 513
Size: 3488
BanI G'GyrC_C
Cuts at: 504 918 1166 1201 504
Size: 414 248 35 2791
Fragments arranged by size:
2791 414 248 35
BanII G_rGCy'C
Cuts at: 380 423 502 1532 380
Size: 43 79 1030 2336
Fragments arranged by size:
2336 1030 79 43
BbeI G_GCGC'C
Cuts at: 922 1170 922
Size: 248 3240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3454
BciVI GTATCCnnnnn_n'
Cuts at: 1386 2147 3488 1386
Size: 761 1341 1386
Fragments arranged by size:
1386 1341 761
BclI T'GATC_A
Cuts at: 1008 1008
Size: 3488
BglII A'GATC_T
Cuts at: 1003 3040 1003
Size: 2037 1451
BlpI GC'TnA_GC
Cuts at: 600 600
Size: 3488
Bme1580I G_kGCm'C
Cuts at: 1113 1206 2262 1113
Size: 93 1056 2339
Fragments arranged by size:
2339 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 166 700 781 1116 3260 166
Size: 534 81 335 2144 394
Fragments arranged by size:
2144 534 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 446
Size: 3488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 664 1543 2035 2333 407
Size: 257 879 492 298 1562
Fragments arranged by size:
1562 879 492 298 257
BsaI GGTCTCn'nnnn_
Cuts at: 330 3192 330
Size: 2862 626
BsaAI yAC'GTr
Cuts at: 841 841
Size: 3488
BsaBI GATnn'nnATC
Cuts at: 6 1002 3039 6
Size: 996 2037 455
Fragments arranged by size:
2037 996 455
BsaHI Gr'CG_yC
Cuts at: 919 1167 1869 3365 919
Size: 248 702 1496 1042
Fragments arranged by size:
1496 1042 702 248
BsaWI w'CCGG_w
Cuts at: 677 1198 1876 2150 2297 2708 677
Size: 521 678 274 147 411 1457
Fragments arranged by size:
1457 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 315 913 1671 2248 2744 315
Size: 598 758 577 496 1059
Fragments arranged by size:
1059 758 598 577 496
BsiEI CG_ry'CG
Cuts at: 104 1030 1076 2284 104
Size: 926 46 1208 1308
Fragments arranged by size:
1308 1208 926 46
BsiHKAI G_wGCw'C
Cuts at: 380 423 502 1280 1470 2262 380
Size: 43 79 778 190 792 1606
Fragments arranged by size:
1606 792 778 190 79 43
Bsp1286I G_dGCh'C
Cuts at: 380 423 502 1113 1206 1280 1470 1532 2262
Size: 43 79 611 93 74 190 62 730
Cuts at: 2262 380
Size: 1606
Fragments arranged by size:
1606 730 611 190 93 79 74 62
43
BspEI T'CCGG_A
Cuts at: 2708 2708
Size: 3488
BspHI T'CATG_A
Cuts at: 2664 2664
Size: 3488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 752 1054 1435 538
Size: 214 302 381 2591
Fragments arranged by size:
2591 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1780 1834 3318 3483 50
Size: 1730 54 1484 165 55
Fragments arranged by size:
1730 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1400 1400
Size: 3488
BsrFI r'CCGG_y
Cuts at: 1486 1667 1876 1486
Size: 181 209 3098
Fragments arranged by size:
3098 209 181
BssSI C'ACGA_G
Cuts at: 1759 2117 1759
Size: 358 3130
BstAPI GCAn_nnn'nTGC
Cuts at: 865 865
Size: 3488
BstBI TT'CG_AA
Cuts at: 1849 1849
Size: 3488
BstYI r'GATC_y
Cuts at: 1 513 980 1003 1338 1584 2596 2682 2694
Size: 512 467 23 335 246 1012 86 12
Cuts at: 2694 3040 1
Size: 346 449
Fragments arranged by size:
1012 512 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 116 116
Size: 3488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1438 1619 1782 1438
Size: 181 163 3144
Fragments arranged by size:
3144 181 163
BtsI GCAGTG_nn'
Cuts at: 731 731
Size: 3488
DraI TTT'AAA
Cuts at: 487 487
Size: 3488
DrdI GACnn_nn'nnGTC
Cuts at: 1194 2052 1194
Size: 858 2630
EaeI y'GGCC_r
Cuts at: 152 356 1073 1247 1638 1665 3010 152
Size: 204 717 174 391 27 1345 630
Fragments arranged by size:
1345 717 630 391 204 174 27
EagI C'GGCC_G
Cuts at: 1073 1073
Size: 3488
EarI CTCTTCn'nnn_
Cuts at: 1511 1721 1511
Size: 210 3278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1696 2006 2152 3298 1696
Size: 310 146 1146 1886
Fragments arranged by size:
1886 1146 310 146
EcoICRI GAG'CTC
Cuts at: 378 421 500 378
Size: 43 79 3366
Fragments arranged by size:
3366 79 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 358 446 1313 1745 2492 2760 355
Size: 3 88 867 432 747 268 1083
Fragments arranged by size:
1083 867 747 432 268 88 3
EcoO109I rG'GnC_Cy
Cuts at: 627 627
Size: 3488
EcoRI G'AATT_C
Cuts at: 492 492
Size: 3488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1507 1507
Size: 3488
FspI TGC'GCA
Cuts at: 1269 1269
Size: 3488
HaeII r_GCGC'y
Cuts at: 922 1170 2192 3171 922
Size: 248 1022 979 1239
Fragments arranged by size:
1239 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1330 1360 1362 1392 1330
Size: 30 2 30 3426
Fragments arranged by size:
3426 30 30 2
HincII GTy'rAC
Cuts at: 527 527
Size: 3488
KasI G'GCGC_C
Cuts at: 918 1166 918
Size: 248 3240
KpnI G_GTAC'C
Cuts at: 508 508
Size: 3488
MluI A'CGCG_T
Cuts at: 2921 2921
Size: 3488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2343 2159
Size: 184 3304
MscI TGG'CCA
Cuts at: 358 1249 358
Size: 891 2597
MslI CAynn'nnrTG
Cuts at: 1477 1604 1477
Size: 127 3361
MspA1I CmG'CkG
Cuts at: 319 466 599 913 1273 2286 2531 319
Size: 147 133 314 360 1013 245 1276
Fragments arranged by size:
1276 1013 360 314 245 147 133
NaeI GCC'GGC
Cuts at: 1669 1669
Size: 3488
NarI GG'CG_CC
Cuts at: 919 1167 919
Size: 248 3240
NgoMIV G'CCGG_C
Cuts at: 1667 1667
Size: 3488
NspI r_CATG'y
Cuts at: 541 1041 1948 541
Size: 500 907 2081
Fragments arranged by size:
2081 907 500
PciI A'CATG_T
Cuts at: 1944 1944
Size: 3488
PflMI CCAn_nnn'nTGG
Cuts at: 469 469
Size: 3488
PmeI GTTT'AAAC
Cuts at: 487 487
Size: 3488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 3456
PsiI TTA'TAA
Cuts at: 310 310
Size: 3488
PstI C_TGCA'G
Cuts at: 535 1220 535
Size: 685 2803
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3488
PvuII CAG'CTG
Cuts at: 319 466 913 1273 319
Size: 147 447 360 2534
Fragments arranged by size:
2534 447 360 147
RsrII CG'GwC_CG
Cuts at: 1683 1683
Size: 3488
SacI G_AGCT'C
Cuts at: 380 423 502 380
Size: 43 79 3366
Fragments arranged by size:
3366 79 43
SalI G'TCGA_C
Cuts at: 525 525
Size: 3488
SapI GCTCTTCn'nnn_
Cuts at: 1511 1721 1511
Size: 210 3278
SbfI CC_TGCA'GG
Cuts at: 535 535
Size: 3488
ScaI AGT'ACT
Cuts at: 3005 3005
Size: 3488
SfcI C'TryA_G
Cuts at: 33 531 1216 2209 2400 33
Size: 498 685 993 191 1121
Fragments arranged by size:
1121 993 685 498 191
SfoI GGC'GCC
Cuts at: 920 1168 920
Size: 248 3240
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
SmaI CCC'GGG
Cuts at: 510 510
Size: 3488
SmlI C'TyrA_G
Cuts at: 422 643 1558 2050 2312 422
Size: 221 915 492 262 1598
Fragments arranged by size:
1598 915 492 262 221
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3488
SphI G_CATG'C
Cuts at: 541 1041 541
Size: 500 2988
StyI C'CwwG_G
Cuts at: 446 622 976 446
Size: 176 354 2958
Fragments arranged by size:
2958 354 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1071 1204 1663 1872 1071
Size: 133 459 209 2687
Fragments arranged by size:
2687 459 209 133
TatI w'GTAC_w
Cuts at: 3003 3003
Size: 3488
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 3456
Tth111I GACn'n_nGTC
Cuts at: 1285 1285
Size: 3488
XbaI T'CTAG_A
Cuts at: 519 519
Size: 3488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 983 983
Size: 3488
XmaI C'CCGG_G
Cuts at: 508 508
Size: 3488
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI