Restriction map for
pF1KB6774
(Circular) (Six-base) MAPSORT of: pF1KB6774.seq Check: 2070 from: 1 to: 3566
pF1KB6774 3566 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 604 604
Size: 3566
Acc65I G'GTAC_C
Cuts at: 582 582
Size: 3566
AclI AA'CG_TT
Cuts at: 3406 3406
Size: 3566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1391 1823 2570 2838 1391
Size: 432 747 268 2119
Fragments arranged by size:
2119 747 432 268
AflIII A'CryG_T
Cuts at: 918 2022 2999 918
Size: 1104 977 1485
Fragments arranged by size:
1485 1104 977
AgeI A'CCGG_T
Cuts at: 1954 1954
Size: 3566
AlwNI CAG_nnn'CTG
Cuts at: 871 2438 871
Size: 1567 1999
ApaI G_GGCC'C
Cuts at: 475 475
Size: 3566
ApaLI G'TGCA_C
Cuts at: 2336 2336
Size: 3566
ApoI r'AATT_y
Cuts at: 570 2919 3175 570
Size: 2349 256 961
Fragments arranged by size:
2349 961 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3566
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
AvaI C'yCGr_G
Cuts at: 124 586 124
Size: 462 3104
BamHI G'GATC_C
Cuts at: 591 591
Size: 3566
BanI G'GyrC_C
Cuts at: 178 582 996 1244 1279 178
Size: 404 414 248 35 2465
Fragments arranged by size:
2465 414 404 248 35
BanII G_rGCy'C
Cuts at: 125 475 580 1610 125
Size: 350 105 1030 2081
Fragments arranged by size:
2081 1030 350 105
BbeI G_GCGC'C
Cuts at: 1000 1248 1000
Size: 248 3318
BbsI GAAGACnn'nnnn_
Cuts at: 353 353
Size: 3566
BciVI GTATCCnnnnn_n'
Cuts at: 1464 2225 3566 1464
Size: 761 1341 1464
Fragments arranged by size:
1464 1341 761
BclI T'GATC_A
Cuts at: 1086 1086
Size: 3566
BglI GCCn_nnn'nGGC
Cuts at: 496 496
Size: 3566
BglII A'GATC_T
Cuts at: 1081 3118 1081
Size: 2037 1529
BlpI GC'TnA_GC
Cuts at: 415 678 415
Size: 263 3303
Bme1580I G_kGCm'C
Cuts at: 475 1191 1284 2340 475
Size: 716 93 1056 1701
Fragments arranged by size:
1701 1056 716 93
BmrI ACTGGGnnnn_n'
Cuts at: 778 859 1194 3338 778
Size: 81 335 2144 1006
Fragments arranged by size:
2144 1006 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1933 1933
Size: 3566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 229 240 382 478 525 190
Size: 39 11 142 96 47 3231
Fragments arranged by size:
3231 142 96 47 39 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1621 2113 2411 742
Size: 879 492 298 1897
Fragments arranged by size:
1897 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3270 3270
Size: 3566
BsaAI yAC'GTr
Cuts at: 919 919
Size: 3566
BsaBI GATnn'nnATC
Cuts at: 6 1080 3117 6
Size: 1074 2037 455
Fragments arranged by size:
2037 1074 455
BsaHI Gr'CG_yC
Cuts at: 997 1245 1947 3443 997
Size: 248 702 1496 1120
Fragments arranged by size:
1496 1120 702 248
BsaWI w'CCGG_w
Cuts at: 755 1276 1954 2228 2375 2786 755
Size: 521 678 274 147 411 1535
Fragments arranged by size:
1535 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 259 289 259
Size: 30 3536
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 267 379 267
Size: 112 3454
BseYI C'CCAG_C
Cuts at: 548 991 1749 2326 2822 548
Size: 443 758 577 496 1292
Fragments arranged by size:
1292 758 577 496 443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 550
Size: 3566
BsiEI CG_ry'CG
Cuts at: 104 1108 1154 2362 104
Size: 1004 46 1208 1308
Fragments arranged by size:
1308 1208 1004 46
BsiHKAI G_wGCw'C
Cuts at: 176 580 1358 1548 2340 176
Size: 404 778 190 792 1402
Fragments arranged by size:
1402 792 778 404 190
Bsp1286I G_dGCh'C
Cuts at: 125 176 475 580 1191 1284 1358 1548 1610
Size: 51 299 105 611 93 74 190 62
Cuts at: 1610 2340 125
Size: 730 1351
Fragments arranged by size:
1351 730 611 299 190 105 93 74
62 51
BspEI T'CCGG_A
Cuts at: 2786 2786
Size: 3566
BspHI T'CATG_A
Cuts at: 2742 2742
Size: 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 227 616 830 1132 1513 227
Size: 389 214 302 381 2280
Fragments arranged by size:
2280 389 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1858 1912 3396 3561 50
Size: 1808 54 1484 165 55
Fragments arranged by size:
1808 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1478 1478
Size: 3566
BsrFI r'CCGG_y
Cuts at: 1564 1745 1954 1564
Size: 181 209 3176
Fragments arranged by size:
3176 209 181
BssSI C'ACGA_G
Cuts at: 316 1837 2195 316
Size: 1521 358 1687
Fragments arranged by size:
1687 1521 358
BstAPI GCAn_nnn'nTGC
Cuts at: 943 943
Size: 3566
BstBI TT'CG_AA
Cuts at: 1927 1927
Size: 3566
BstYI r'GATC_y
Cuts at: 1 591 1058 1081 1416 1662 2674 2760 2772
Size: 590 467 23 335 246 1012 86 12
Cuts at: 2772 3118 1
Size: 346 449
Fragments arranged by size:
1012 590 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3566
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1516 1697 1860 1516
Size: 181 163 3222
Fragments arranged by size:
3222 181 163
BtsI GCAGTG_nn'
Cuts at: 158 338 407 809 158
Size: 180 69 402 2915
Fragments arranged by size:
2915 402 180 69
DraI TTT'AAA
Cuts at: 565 565
Size: 3566
DrdI GACnn_nn'nnGTC
Cuts at: 1272 2130 1272
Size: 858 2708
EaeI y'GGCC_r
Cuts at: 1151 1325 1716 1743 3088 1151
Size: 174 391 27 1345 1629
Fragments arranged by size:
1629 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1151 1151
Size: 3566
EarI CTCTTCn'nnn_
Cuts at: 235 1589 1799 235
Size: 1354 210 2002
Fragments arranged by size:
2002 1354 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1774 2084 2230 3376 1774
Size: 310 146 1146 1964
Fragments arranged by size:
1964 1146 310 146
EcoICRI GAG'CTC
Cuts at: 578 578
Size: 3566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 229 240 382 478 525 1391 1823 2570
Size: 39 11 142 96 47 866 432 747
Cuts at: 2570 2838 190
Size: 268 918
Fragments arranged by size:
918 866 747 432 268 142 96 47
39 11
EcoO109I rG'GnC_Cy
Cuts at: 472 705 472
Size: 233 3333
EcoRI G'AATT_C
Cuts at: 570 570
Size: 3566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1585 1585
Size: 3566
FspI TGC'GCA
Cuts at: 1347 1347
Size: 3566
HaeII r_GCGC'y
Cuts at: 117 1000 1248 2270 3249 117
Size: 883 248 1022 979 434
Fragments arranged by size:
1022 979 883 434 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 340 372 1408 1438 1440 1470 259
Size: 32 49 32 1036 30 2 30 2355
Fragments arranged by size:
2355 1036 49 32 32 30 30 2
HincII GTy'rAC
Cuts at: 605 605
Size: 3566
KasI G'GCGC_C
Cuts at: 996 1244 996
Size: 248 3318
KpnI G_GTAC'C
Cuts at: 586 586
Size: 3566
MluI A'CGCG_T
Cuts at: 2999 2999
Size: 3566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2421 2237
Size: 184 3382
MscI TGG'CCA
Cuts at: 1327 1327
Size: 3566
MslI CAynn'nnrTG
Cuts at: 1555 1682 1555
Size: 127 3439
MspA1I CmG'CkG
Cuts at: 168 677 991 1351 2364 2609 168
Size: 509 314 360 1013 245 1125
Fragments arranged by size:
1125 1013 509 360 314 245
NaeI GCC'GGC
Cuts at: 1747 1747
Size: 3566
NarI GG'CG_CC
Cuts at: 997 1245 997
Size: 248 3318
NcoI C'CATG_G
Cuts at: 107 107
Size: 3566
NgoMIV G'CCGG_C
Cuts at: 1745 1745
Size: 3566
NspI r_CATG'y
Cuts at: 456 619 1119 2026 456
Size: 163 500 907 1996
Fragments arranged by size:
1996 907 500 163
PciI A'CATG_T
Cuts at: 2022 2022
Size: 3566
PfoI T'CCnGG_A
Cuts at: 290 290
Size: 3566
PmeI GTTT'AAAC
Cuts at: 565 565
Size: 3566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2705 2737 2705
Size: 32 3534
PspOMI G'GGCC_C
Cuts at: 471 471
Size: 3566
PstI C_TGCA'G
Cuts at: 333 613 1298 333
Size: 280 685 2601
Fragments arranged by size:
2601 685 280
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3566
PvuII CAG'CTG
Cuts at: 991 1351 991
Size: 360 3206
RsrII CG'GwC_CG
Cuts at: 1761 1761
Size: 3566
SacI G_AGCT'C
Cuts at: 580 580
Size: 3566
SalI G'TCGA_C
Cuts at: 603 603
Size: 3566
SapI GCTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
SbfI CC_TGCA'GG
Cuts at: 613 613
Size: 3566
ScaI AGT'ACT
Cuts at: 3083 3083
Size: 3566
SfcI C'TryA_G
Cuts at: 33 329 609 1294 2287 2478 33
Size: 296 280 685 993 191 1121
Fragments arranged by size:
1121 993 685 296 280 191
SfoI GGC'GCC
Cuts at: 998 1246 998
Size: 248 3318
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
SmaI CCC'GGG
Cuts at: 588 588
Size: 3566
SmlI C'TyrA_G
Cuts at: 721 1636 2128 2390 721
Size: 915 492 262 1897
Fragments arranged by size:
1897 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3566
SphI G_CATG'C
Cuts at: 619 1119 619
Size: 500 3066
StyI C'CwwG_G
Cuts at: 107 700 1054 107
Size: 593 354 2619
Fragments arranged by size:
2619 593 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 394 424 1149 1282 1741 1950 394
Size: 30 725 133 459 209 2010
Fragments arranged by size:
2010 725 459 209 133 30
TatI w'GTAC_w
Cuts at: 236 3081 236
Size: 2845 721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 3534
Tth111I GACn'n_nGTC
Cuts at: 1363 1363
Size: 3566
XbaI T'CTAG_A
Cuts at: 597 597
Size: 3566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1061 1061
Size: 3566
XmaI C'CCGG_G
Cuts at: 586 586
Size: 3566
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI