Restriction map for
pF1KB6770
(Circular) (Six-base) MAPSORT of: pF1KB6770.seq Check: 552 from: 1 to: 3563
pF1KB6770 3563 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 601 601
Size: 3563
Acc65I G'GTAC_C
Cuts at: 579 579
Size: 3563
AclI AA'CG_TT
Cuts at: 485 3403 485
Size: 2918 645
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1388 1820 2567 2835 1388
Size: 432 747 268 2116
Fragments arranged by size:
2116 747 432 268
AflIII A'CryG_T
Cuts at: 915 2019 2996 915
Size: 1104 977 1482
Fragments arranged by size:
1482 1104 977
AgeI A'CCGG_T
Cuts at: 1951 1951
Size: 3563
AlwNI CAG_nnn'CTG
Cuts at: 868 2435 868
Size: 1567 1996
ApaLI G'TGCA_C
Cuts at: 2333 2333
Size: 3563
ApoI r'AATT_y
Cuts at: 325 567 2916 3172 325
Size: 242 2349 256 716
Fragments arranged by size:
2349 716 256 242
AseI AT'TA_AT
Cuts at: 20 20
Size: 3563
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3563
AvaI C'yCGr_G
Cuts at: 287 445 583 287
Size: 158 138 3267
Fragments arranged by size:
3267 158 138
BamHI G'GATC_C
Cuts at: 588 588
Size: 3563
BanI G'GyrC_C
Cuts at: 579 993 1241 1276 579
Size: 414 248 35 2866
Fragments arranged by size:
2866 414 248 35
BanII G_rGCy'C
Cuts at: 577 1607 577
Size: 1030 2533
BbeI G_GCGC'C
Cuts at: 997 1245 997
Size: 248 3315
BbsI GAAGACnn'nnnn_
Cuts at: 221 221
Size: 3563
BciVI GTATCCnnnnn_n'
Cuts at: 1461 2222 3563 1461
Size: 761 1341 1461
Fragments arranged by size:
1461 1341 761
BclI T'GATC_A
Cuts at: 1083 1083
Size: 3563
BglII A'GATC_T
Cuts at: 1078 3115 1078
Size: 2037 1526
BlpI GC'TnA_GC
Cuts at: 675 675
Size: 3563
Bme1580I G_kGCm'C
Cuts at: 1188 1281 2337 1188
Size: 93 1056 2414
Fragments arranged by size:
2414 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 488 775 856 1191 3335 488
Size: 287 81 335 2144 716
Fragments arranged by size:
2144 716 335 287 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1930 1930
Size: 3563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 739 1618 2110 2408 253
Size: 486 879 492 298 1408
Fragments arranged by size:
1408 879 492 486 298
BsaI GGTCTCn'nnnn_
Cuts at: 3267 3267
Size: 3563
BsaAI yAC'GTr
Cuts at: 916 916
Size: 3563
BsaBI GATnn'nnATC
Cuts at: 6 1077 3114 6
Size: 1071 2037 455
Fragments arranged by size:
2037 1071 455
BsaHI Gr'CG_yC
Cuts at: 994 1242 1944 3440 994
Size: 248 702 1496 1117
Fragments arranged by size:
1496 1117 702 248
BsaWI w'CCGG_w
Cuts at: 752 1273 1951 2225 2372 2783 752
Size: 521 678 274 147 411 1532
Fragments arranged by size:
1532 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 988 1746 2323 2819 988
Size: 758 577 496 1732
Fragments arranged by size:
1732 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1105 1151 2359 104
Size: 1001 46 1208 1308
Fragments arranged by size:
1308 1208 1001 46
BsiHKAI G_wGCw'C
Cuts at: 577 1355 1545 2337 577
Size: 778 190 792 1803
Fragments arranged by size:
1803 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 577 1188 1281 1355 1545 1607 2337 577
Size: 611 93 74 190 62 730 1803
Fragments arranged by size:
1803 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2783 2783
Size: 3563
BspHI T'CATG_A
Cuts at: 2739 2739
Size: 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 613 827 1129 1510 613
Size: 214 302 381 2666
Fragments arranged by size:
2666 381 302 214
BsrBI CCG'CTC
Cuts at: 50 126 1855 1909 3393 3558 50
Size: 76 1729 54 1484 165 55
Fragments arranged by size:
1729 1484 165 76 55 54
BsrDI GCAATG_nn'
Cuts at: 367 1475 367
Size: 1108 2455
BsrFI r'CCGG_y
Cuts at: 1561 1742 1951 1561
Size: 181 209 3173
Fragments arranged by size:
3173 209 181
BssSI C'ACGA_G
Cuts at: 1834 2192 1834
Size: 358 3205
BstAPI GCAn_nnn'nTGC
Cuts at: 940 940
Size: 3563
BstBI TT'CG_AA
Cuts at: 1924 1924
Size: 3563
BstYI r'GATC_y
Cuts at: 1 137 588 1055 1078 1413 1659 2671 2757
Size: 136 451 467 23 335 246 1012 86
Cuts at: 2757 2769 3115 1
Size: 12 346 449
Fragments arranged by size:
1012 467 451 449 346 335 246 136
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 142 142
Size: 3563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1513 1694 1857 1513
Size: 181 163 3219
Fragments arranged by size:
3219 181 163
BtsI GCAGTG_nn'
Cuts at: 287 806 287
Size: 519 3044
DraI TTT'AAA
Cuts at: 562 562
Size: 3563
DrdI GACnn_nn'nnGTC
Cuts at: 223 1269 2127 223
Size: 1046 858 1659
Fragments arranged by size:
1659 1046 858
EaeI y'GGCC_r
Cuts at: 1148 1322 1713 1740 3085 1148
Size: 174 391 27 1345 1626
Fragments arranged by size:
1626 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1148 1148
Size: 3563
EarI CTCTTCn'nnn_
Cuts at: 1586 1796 1586
Size: 210 3353
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 319 1771 2081 2227 3373 319
Size: 1452 310 146 1146 509
Fragments arranged by size:
1452 1146 509 310 146
EcoICRI GAG'CTC
Cuts at: 575 575
Size: 3563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1388 1820 2567 2835 1388
Size: 432 747 268 2116
Fragments arranged by size:
2116 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 702 702
Size: 3563
EcoRI G'AATT_C
Cuts at: 567 567
Size: 3563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1582 1582
Size: 3563
FspI TGC'GCA
Cuts at: 1344 1344
Size: 3563
HaeII r_GCGC'y
Cuts at: 997 1245 2267 3246 997
Size: 248 1022 979 1314
Fragments arranged by size:
1314 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1405 1435 1437 1467 1405
Size: 30 2 30 3501
Fragments arranged by size:
3501 30 30 2
HincII GTy'rAC
Cuts at: 602 602
Size: 3563
KasI G'GCGC_C
Cuts at: 993 1241 993
Size: 248 3315
KpnI G_GTAC'C
Cuts at: 583 583
Size: 3563
MluI A'CGCG_T
Cuts at: 2996 2996
Size: 3563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2418 2234
Size: 184 3379
MscI TGG'CCA
Cuts at: 1324 1324
Size: 3563
MslI CAynn'nnrTG
Cuts at: 1552 1679 1552
Size: 127 3436
MspA1I CmG'CkG
Cuts at: 674 988 1348 2361 2606 674
Size: 314 360 1013 245 1631
Fragments arranged by size:
1631 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1744 1744
Size: 3563
NarI GG'CG_CC
Cuts at: 994 1242 994
Size: 248 3315
NgoMIV G'CCGG_C
Cuts at: 1742 1742
Size: 3563
NspI r_CATG'y
Cuts at: 616 1116 2023 616
Size: 500 907 2156
Fragments arranged by size:
2156 907 500
PciI A'CATG_T
Cuts at: 2019 2019
Size: 3563
PmeI GTTT'AAAC
Cuts at: 562 562
Size: 3563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2702 2734 2702
Size: 32 3531
PstI C_TGCA'G
Cuts at: 610 1295 610
Size: 685 2878
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3563
PvuII CAG'CTG
Cuts at: 988 1348 988
Size: 360 3203
RsrII CG'GwC_CG
Cuts at: 1758 1758
Size: 3563
SacI G_AGCT'C
Cuts at: 577 577
Size: 3563
SalI G'TCGA_C
Cuts at: 600 600
Size: 3563
SapI GCTCTTCn'nnn_
Cuts at: 1586 1796 1586
Size: 210 3353
SbfI CC_TGCA'GG
Cuts at: 610 610
Size: 3563
ScaI AGT'ACT
Cuts at: 3080 3080
Size: 3563
SfcI C'TryA_G
Cuts at: 33 606 1291 2284 2475 33
Size: 573 685 993 191 1121
Fragments arranged by size:
1121 993 685 573 191
SfoI GGC'GCC
Cuts at: 995 1243 995
Size: 248 3315
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3563
SmaI CCC'GGG
Cuts at: 585 585
Size: 3563
SmlI C'TyrA_G
Cuts at: 268 287 718 1633 2125 2387 268
Size: 19 431 915 492 262 1444
Fragments arranged by size:
1444 915 492 431 262 19
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3563
SphI G_CATG'C
Cuts at: 616 1116 616
Size: 500 3063
StyI C'CwwG_G
Cuts at: 116 697 1051 116
Size: 581 354 2628
Fragments arranged by size:
2628 581 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1146 1279 1738 1947 1146
Size: 133 459 209 2762
Fragments arranged by size:
2762 459 209 133
TatI w'GTAC_w
Cuts at: 3078 3078
Size: 3563
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3080 3112 3080
Size: 32 3531
Tth111I GACn'n_nGTC
Cuts at: 1360 1360
Size: 3563
XbaI T'CTAG_A
Cuts at: 594 594
Size: 3563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1058 1058
Size: 3563
XhoI C'TCGA_G
Cuts at: 287 287
Size: 3563
XmaI C'CCGG_G
Cuts at: 583 583
Size: 3563
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI