Restriction map for
pF1KB6752
(Circular) (Six-base) MAPSORT of: pF1KB6752.seq Check: 1986 from: 1 to: 3548
pF1KB6752 3548 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 353 353
Size: 3548
AccI GT'mk_AC
Cuts at: 395 586 395
Size: 191 3357
Acc65I G'GTAC_C
Cuts at: 564 564
Size: 3548
AclI AA'CG_TT
Cuts at: 3388 3388
Size: 3548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1373 1805 2552 2820 298
Size: 1075 432 747 268 1026
Fragments arranged by size:
1075 1026 747 432 268
AflIII A'CryG_T
Cuts at: 188 900 2004 2981 188
Size: 712 1104 977 755
Fragments arranged by size:
1104 977 755 712
AgeI A'CCGG_T
Cuts at: 1936 1936
Size: 3548
AlwNI CAG_nnn'CTG
Cuts at: 183 853 2420 183
Size: 670 1567 1311
Fragments arranged by size:
1567 1311 670
ApaLI G'TGCA_C
Cuts at: 2318 2318
Size: 3548
ApoI r'AATT_y
Cuts at: 552 2901 3157 552
Size: 2349 256 943
Fragments arranged by size:
2349 943 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3548
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3548
AvaI C'yCGr_G
Cuts at: 367 433 568 367
Size: 66 135 3347
Fragments arranged by size:
3347 135 66
BamHI G'GATC_C
Cuts at: 573 573
Size: 3548
BanI G'GyrC_C
Cuts at: 306 564 978 1226 1261 306
Size: 258 414 248 35 2593
Fragments arranged by size:
2593 414 258 248 35
BanII G_rGCy'C
Cuts at: 562 1592 562
Size: 1030 2518
BbeI G_GCGC'C
Cuts at: 310 982 1230 310
Size: 672 248 2628
Fragments arranged by size:
2628 672 248
BbsI GAAGACnn'nnnn_
Cuts at: 343 343
Size: 3548
BciVI GTATCCnnnnn_n'
Cuts at: 1446 2207 3548 1446
Size: 761 1341 1446
Fragments arranged by size:
1446 1341 761
BclI T'GATC_A
Cuts at: 356 1068 356
Size: 712 2836
BglI GCCn_nnn'nGGC
Cuts at: 500 500
Size: 3548
BglII A'GATC_T
Cuts at: 1063 3100 1063
Size: 2037 1511
BlpI GC'TnA_GC
Cuts at: 660 660
Size: 3548
Bme1580I G_kGCm'C
Cuts at: 1173 1266 2322 1173
Size: 93 1056 2399
Fragments arranged by size:
2399 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 760 841 1176 3320 760
Size: 81 335 2144 988
Fragments arranged by size:
2144 988 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 724 1603 2095 2393 523
Size: 201 879 492 298 1678
Fragments arranged by size:
1678 879 492 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 3252 3252
Size: 3548
BsaAI yAC'GTr
Cuts at: 901 901
Size: 3548
BsaBI GATnn'nnATC
Cuts at: 6 1062 3099 6
Size: 1056 2037 455
Fragments arranged by size:
2037 1056 455
BsaHI Gr'CG_yC
Cuts at: 307 392 979 1227 1929 3425 307
Size: 85 587 248 702 1496 430
Fragments arranged by size:
1496 702 587 430 248 85
BsaWI w'CCGG_w
Cuts at: 737 1258 1936 2210 2357 2768 737
Size: 521 678 274 147 411 1517
Fragments arranged by size:
1517 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 510 510
Size: 3548
BseYI C'CCAG_C
Cuts at: 973 1731 2308 2804 973
Size: 758 577 496 1717
Fragments arranged by size:
1717 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1090 1136 2344 104
Size: 986 46 1208 1308
Fragments arranged by size:
1308 1208 986 46
BsiHKAI G_wGCw'C
Cuts at: 562 1340 1530 2322 562
Size: 778 190 792 1788
Fragments arranged by size:
1788 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 562 1173 1266 1340 1530 1592 2322 562
Size: 611 93 74 190 62 730 1788
Fragments arranged by size:
1788 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2768 2768
Size: 3548
BspHI T'CATG_A
Cuts at: 2724 2724
Size: 3548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 353 598 812 1114 1495 353
Size: 245 214 302 381 2406
Fragments arranged by size:
2406 381 302 245 214
BsrBI CCG'CTC
Cuts at: 50 1840 1894 3378 3543 50
Size: 1790 54 1484 165 55
Fragments arranged by size:
1790 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1460 1460
Size: 3548
BsrFI r'CCGG_y
Cuts at: 494 1546 1727 1936 494
Size: 1052 181 209 2106
Fragments arranged by size:
2106 1052 209 181
BsrGI T'GTAC_A
Cuts at: 215 215
Size: 3548
BssHII G'CGCG_C
Cuts at: 223 223
Size: 3548
BssSI C'ACGA_G
Cuts at: 1819 2177 1819
Size: 358 3190
BstAPI GCAn_nnn'nTGC
Cuts at: 207 273 925 207
Size: 66 652 2830
Fragments arranged by size:
2830 652 66
BstBI TT'CG_AA
Cuts at: 1909 1909
Size: 3548
BstYI r'GATC_y
Cuts at: 1 573 1040 1063 1398 1644 2656 2742 2754
Size: 572 467 23 335 246 1012 86 12
Cuts at: 2754 3100 1
Size: 346 449
Fragments arranged by size:
1012 572 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 159 314 107
Size: 52 155 3341
Fragments arranged by size:
3341 155 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1498 1679 1842 1498
Size: 181 163 3204
Fragments arranged by size:
3204 181 163
BtsI GCAGTG_nn'
Cuts at: 791 791
Size: 3548
DraI TTT'AAA
Cuts at: 547 547
Size: 3548
DrdI GACnn_nn'nnGTC
Cuts at: 1254 2112 1254
Size: 858 2690
EaeI y'GGCC_r
Cuts at: 444 492 1133 1307 1698 1725 3070 444
Size: 48 641 174 391 27 1345 922
Fragments arranged by size:
1345 922 641 391 174 48 27
EagI C'GGCC_G
Cuts at: 1133 1133
Size: 3548
EarI CTCTTCn'nnn_
Cuts at: 1571 1781 1571
Size: 210 3338
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 273 1756 2066 2212 3358 273
Size: 1483 310 146 1146 463
Fragments arranged by size:
1483 1146 463 310 146
EcoICRI GAG'CTC
Cuts at: 560 560
Size: 3548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1373 1805 2552 2820 298
Size: 1075 432 747 268 1026
Fragments arranged by size:
1075 1026 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 289 289
Size: 3548
EcoO109I rG'GnC_Cy
Cuts at: 249 687 249
Size: 438 3110
EcoRI G'AATT_C
Cuts at: 552 552
Size: 3548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 156 1567 156
Size: 1411 2137
FseI GG_CCGG'CC
Cuts at: 498 498
Size: 3548
FspI TGC'GCA
Cuts at: 289 1329 289
Size: 1040 2508
HaeII r_GCGC'y
Cuts at: 285 310 982 1230 2252 3231 285
Size: 25 672 248 1022 979 602
Fragments arranged by size:
1022 979 672 602 248 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1390 1420 1422 1452 1390
Size: 30 2 30 3486
Fragments arranged by size:
3486 30 30 2
HincII GTy'rAC
Cuts at: 587 587
Size: 3548
KasI G'GCGC_C
Cuts at: 306 978 1226 306
Size: 672 248 2628
Fragments arranged by size:
2628 672 248
KpnI G_GTAC'C
Cuts at: 568 568
Size: 3548
MluI A'CGCG_T
Cuts at: 2981 2981
Size: 3548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2219 2403 2219
Size: 184 3364
MscI TGG'CCA
Cuts at: 1309 1309
Size: 3548
MslI CAynn'nnrTG
Cuts at: 1537 1664 1537
Size: 127 3421
MspA1I CmG'CkG
Cuts at: 161 180 204 213 231 659 973 1333 2346
Size: 19 24 9 18 428 314 360 1013
Cuts at: 2346 2591 161
Size: 245 1118
Fragments arranged by size:
1118 1013 428 360 314 245 24 19
18 9
NaeI GCC'GGC
Cuts at: 496 1729 496
Size: 1233 2315
NarI GG'CG_CC
Cuts at: 307 979 1227 307
Size: 672 248 2628
Fragments arranged by size:
2628 672 248
NcoI C'CATG_G
Cuts at: 107 314 107
Size: 207 3341
NgoMIV G'CCGG_C
Cuts at: 494 1727 494
Size: 1233 2315
NspI r_CATG'y
Cuts at: 192 601 1101 2008 192
Size: 409 500 907 1732
Fragments arranged by size:
1732 907 500 409
PciI A'CATG_T
Cuts at: 188 2004 188
Size: 1816 1732
PflMI CCAn_nnn'nTGG
Cuts at: 183 453 183
Size: 270 3278
PmeI GTTT'AAAC
Cuts at: 547 547
Size: 3548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2687 2719 2687
Size: 32 3516
PstI C_TGCA'G
Cuts at: 595 1280 595
Size: 685 2863
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3548
PvuII CAG'CTG
Cuts at: 180 204 213 973 1333 180
Size: 24 9 760 360 2395
Fragments arranged by size:
2395 760 360 24 9
RsrII CG'GwC_CG
Cuts at: 1743 1743
Size: 3548
SacI G_AGCT'C
Cuts at: 562 562
Size: 3548
SacII CC_GC'GG
Cuts at: 162 162
Size: 3548
SalI G'TCGA_C
Cuts at: 585 585
Size: 3548
SapI GCTCTTCn'nnn_
Cuts at: 1571 1781 1571
Size: 210 3338
SbfI CC_TGCA'GG
Cuts at: 595 595
Size: 3548
ScaI AGT'ACT
Cuts at: 3065 3065
Size: 3548
SexAI A'CCwGG_T
Cuts at: 416 416
Size: 3548
SfcI C'TryA_G
Cuts at: 33 591 1276 2269 2460 33
Size: 558 685 993 191 1121
Fragments arranged by size:
1121 993 685 558 191
SfoI GGC'GCC
Cuts at: 308 980 1228 308
Size: 672 248 2628
Fragments arranged by size:
2628 672 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3548
SmaI CCC'GGG
Cuts at: 570 570
Size: 3548
SmlI C'TyrA_G
Cuts at: 538 703 1618 2110 2372 538
Size: 165 915 492 262 1714
Fragments arranged by size:
1714 915 492 262 165
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3548
SphI G_CATG'C
Cuts at: 601 1101 601
Size: 500 3048
StyI C'CwwG_G
Cuts at: 107 314 682 1036 107
Size: 207 368 354 2619
Fragments arranged by size:
2619 368 354 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 529 1131 1264 1723 1932 529
Size: 602 133 459 209 2145
Fragments arranged by size:
2145 602 459 209 133
TatI w'GTAC_w
Cuts at: 215 3063 215
Size: 2848 700
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 3516
Tth111I GACn'n_nGTC
Cuts at: 1345 1345
Size: 3548
XbaI T'CTAG_A
Cuts at: 579 579
Size: 3548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1043 1043
Size: 3548
XmaI C'CCGG_G
Cuts at: 568 568
Size: 3548
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI