Restriction map for
pF1KB6664
(Circular) (Six-base) MAPSORT of: pF1KB6664.seq Check: 2073 from: 1 to: 4469
pF1KB6664 4469 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1507 1507
Size: 4469
Acc65I G'GTAC_C
Cuts at: 1485 1485
Size: 4469
AclI AA'CG_TT
Cuts at: 293 4309 293
Size: 4016 453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 2294 2726 3473 3741 566
Size: 1728 432 747 268 1294
Fragments arranged by size:
1728 1294 747 432 268
AflII C'TTAA_G
Cuts at: 271 271
Size: 4469
AflIII A'CryG_T
Cuts at: 1821 2925 3902 1821
Size: 1104 977 2388
Fragments arranged by size:
2388 1104 977
AgeI A'CCGG_T
Cuts at: 2857 2857
Size: 4469
AleI CACnn'nnGTG
Cuts at: 982 982
Size: 4469
AlwNI CAG_nnn'CTG
Cuts at: 616 873 1324 1774 3341 616
Size: 257 451 450 1567 1744
Fragments arranged by size:
1744 1567 451 450 257
ApaLI G'TGCA_C
Cuts at: 3239 3239
Size: 4469
ApoI r'AATT_y
Cuts at: 180 468 648 743 1473 3822 4078 180
Size: 288 180 95 730 2349 256 571
Fragments arranged by size:
2349 730 571 288 256 180 95
AseI AT'TA_AT
Cuts at: 20 236 20
Size: 216 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
AvaI C'yCGr_G
Cuts at: 135 1489 135
Size: 1354 3115
AvrII C'CTAG_G
Cuts at: 675 675
Size: 4469
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 917 950 917
Size: 33 4436
BamHI G'GATC_C
Cuts at: 1494 1494
Size: 4469
BanI G'GyrC_C
Cuts at: 145 1485 1899 2147 2182 145
Size: 1340 414 248 35 2432
Fragments arranged by size:
2432 1340 414 248 35
BanII G_rGCy'C
Cuts at: 115 1231 1483 2513 115
Size: 1116 252 1030 2071
Fragments arranged by size:
2071 1116 1030 252
BbeI G_GCGC'C
Cuts at: 1903 2151 1903
Size: 248 4221
BbsI GAAGACnn'nnnn_
Cuts at: 1253 1253
Size: 4469
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3128 4469 2367
Size: 761 1341 2367
Fragments arranged by size:
2367 1341 761
BclI T'GATC_A
Cuts at: 1989 1989
Size: 4469
BglII A'GATC_T
Cuts at: 549 1984 4021 549
Size: 1435 2037 997
Fragments arranged by size:
2037 1435 997
BlpI GC'TnA_GC
Cuts at: 1581 1581
Size: 4469
Bme1580I G_kGCm'C
Cuts at: 148 2094 2187 3243 148
Size: 1946 93 1056 1374
Fragments arranged by size:
1946 1374 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1402 1681 1762 2097 4241 1402
Size: 279 81 335 2144 1630
Fragments arranged by size:
2144 1630 335 279 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2836 2836
Size: 4469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 475
Size: 4469
Bpu10I CC'TnA_GC
Cuts at: 1245 1245
Size: 4469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1645 2524 3016 3314 1645
Size: 879 492 298 2800
Fragments arranged by size:
2800 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 343 4173 343
Size: 3830 639
BsaAI yAC'GTr
Cuts at: 1822 1822
Size: 4469
BsaBI GATnn'nnATC
Cuts at: 6 316 1983 4020 6
Size: 310 1667 2037 455
Fragments arranged by size:
2037 1667 455 310
BsaHI Gr'CG_yC
Cuts at: 1351 1900 2148 2850 4346 1351
Size: 549 248 702 1496 1474
Fragments arranged by size:
1496 1474 702 549 248
BsaWI w'CCGG_w
Cuts at: 1658 2179 2857 3131 3278 3689 1658
Size: 521 678 274 147 411 2438
Fragments arranged by size:
2438 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1312 1342 1312
Size: 30 4439
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 105 1320 105
Size: 1215 3254
BseYI C'CCAG_C
Cuts at: 1074 1894 2652 3229 3725 1074
Size: 820 758 577 496 1818
Fragments arranged by size:
1818 820 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2011 2057 3265 104
Size: 1907 46 1208 1308
Fragments arranged by size:
1907 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1231 1483 2261 2451 3243 1231
Size: 252 778 190 792 2457
Fragments arranged by size:
2457 792 778 252 190
BsmI GAATG_Cn'
Cuts at: 404 404
Size: 4469
Bsp1286I G_dGCh'C
Cuts at: 115 148 1231 1483 2094 2187 2261 2451 2513
Size: 33 1083 252 611 93 74 190 62
Cuts at: 2513 3243 115
Size: 730 1341
Fragments arranged by size:
1341 1083 730 611 252 190 93 74
62 33
BspEI T'CCGG_A
Cuts at: 3689 3689
Size: 4469
BspHI T'CATG_A
Cuts at: 533 3645 533
Size: 3112 1357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 478 1519 1733 2035 2416 478
Size: 1041 214 302 381 2531
Fragments arranged by size:
2531 1041 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2761 2815 4299 4464 50
Size: 2711 54 1484 165 55
Fragments arranged by size:
2711 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1169 2381 1169
Size: 1212 3257
BsrFI r'CCGG_y
Cuts at: 2467 2648 2857 2467
Size: 181 209 4079
Fragments arranged by size:
4079 209 181
BsrGI T'GTAC_A
Cuts at: 602 602
Size: 4469
BssSI C'ACGA_G
Cuts at: 2740 3098 2740
Size: 358 4111
BstAPI GCAn_nnn'nTGC
Cuts at: 1324 1846 1324
Size: 522 3947
BstBI TT'CG_AA
Cuts at: 2830 2830
Size: 4469
BstYI r'GATC_y
Cuts at: 1 131 276 549 1494 1961 1984 2319 2565
Size: 130 145 273 945 467 23 335 246
Cuts at: 2565 3577 3663 3675 4021 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 945 467 449 346 335 273 246
145 130 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4469
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2419 2600 2763 2419
Size: 181 163 4125
Fragments arranged by size:
4125 181 163
BtsI GCAGTG_nn'
Cuts at: 1358 1712 1358
Size: 354 4115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1362 1397 1362
Size: 35 4434
DraI TTT'AAA
Cuts at: 1468 1468
Size: 4469
DraIII CAC_nnn'GTG
Cuts at: 505 505
Size: 4469
DrdI GACnn_nn'nnGTC
Cuts at: 2175 3033 2175
Size: 858 3611
EaeI y'GGCC_r
Cuts at: 381 2054 2228 2619 2646 3991 381
Size: 1673 174 391 27 1345 859
Fragments arranged by size:
1673 1345 859 391 174 27
EagI C'GGCC_G
Cuts at: 2054 2054
Size: 4469
EarI CTCTTCn'nnn_
Cuts at: 755 1142 1236 2492 2702 755
Size: 387 94 1256 210 2522
Fragments arranged by size:
2522 1256 387 210 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2677 2987 3133 4279 2677
Size: 310 146 1146 2867
Fragments arranged by size:
2867 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1229 1481 1229
Size: 252 4217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 566 2294 2726 3473 3741 475
Size: 91 1728 432 747 268 1203
Fragments arranged by size:
1728 1203 747 432 268 91
EcoO109I rG'GnC_Cy
Cuts at: 1368 1608 1368
Size: 240 4229
EcoRI G'AATT_C
Cuts at: 1473 1473
Size: 4469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4469
FspI TGC'GCA
Cuts at: 2250 2250
Size: 4469
HaeII r_GCGC'y
Cuts at: 1903 2151 3173 4152 1903
Size: 248 1022 979 2220
Fragments arranged by size:
2220 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 1243 1275 1312 1344 2311 2341 2343
Size: 32 763 32 37 32 967 30 2
Cuts at: 2343 2373 448
Size: 30 2544
Fragments arranged by size:
2544 967 763 37 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1508 1508
Size: 4469
KasI G'GCGC_C
Cuts at: 1899 2147 1899
Size: 248 4221
KpnI G_GTAC'C
Cuts at: 1489 1489
Size: 4469
MluI A'CGCG_T
Cuts at: 3902 3902
Size: 4469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3140 3324 3140
Size: 184 4285
MscI TGG'CCA
Cuts at: 2230 2230
Size: 4469
MslI CAynn'nnrTG
Cuts at: 982 2458 2585 982
Size: 1476 127 2866
Fragments arranged by size:
2866 1476 127
MspA1I CmG'CkG
Cuts at: 813 907 1580 1894 2254 3267 3512 813
Size: 94 673 314 360 1013 245 1770
Fragments arranged by size:
1770 1013 673 360 314 245 94
NaeI GCC'GGC
Cuts at: 2650 2650
Size: 4469
NarI GG'CG_CC
Cuts at: 1900 2148 1900
Size: 248 4221
NcoI C'CATG_G
Cuts at: 107 107
Size: 4469
NgoMIV G'CCGG_C
Cuts at: 2648 2648
Size: 4469
NspI r_CATG'y
Cuts at: 451 1522 2022 2929 451
Size: 1071 500 907 1991
Fragments arranged by size:
1991 1071 907 500
PciI A'CATG_T
Cuts at: 2925 2925
Size: 4469
PflMI CCAn_nnn'nTGG
Cuts at: 1099 1099
Size: 4469
PfoI T'CCnGG_A
Cuts at: 753 753
Size: 4469
PmeI GTTT'AAAC
Cuts at: 1468 1468
Size: 4469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4437
PshAI GACnn'nnGTC
Cuts at: 355 355
Size: 4469
PstI C_TGCA'G
Cuts at: 418 480 1239 1516 2201 418
Size: 62 759 277 685 2686
Fragments arranged by size:
2686 759 685 277 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4469
PvuII CAG'CTG
Cuts at: 813 907 1894 2254 813
Size: 94 987 360 3028
Fragments arranged by size:
3028 987 360 94
RsrII CG'GwC_CG
Cuts at: 2664 2664
Size: 4469
SacI G_AGCT'C
Cuts at: 1231 1483 1231
Size: 252 4217
SalI G'TCGA_C
Cuts at: 1506 1506
Size: 4469
SapI GCTCTTCn'nnn_
Cuts at: 1236 2492 2702 1236
Size: 1256 210 3003
Fragments arranged by size:
3003 1256 210
SbfI CC_TGCA'GG
Cuts at: 1516 1516
Size: 4469
ScaI AGT'ACT
Cuts at: 3986 3986
Size: 4469
SexAI A'CCwGG_T
Cuts at: 1014 1014
Size: 4469
SfcI C'TryA_G
Cuts at: 33 321 414 476 854 1235 1512 2197 3190
Size: 288 93 62 378 381 277 685 993
Cuts at: 3190 3381 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 381 378 288 277 191
93 62
SfoI GGC'GCC
Cuts at: 1901 2149 1901
Size: 248 4221
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
SmaI CCC'GGG
Cuts at: 137 1491 137
Size: 1354 3115
SmlI C'TyrA_G
Cuts at: 271 1624 2539 3031 3293 271
Size: 1353 915 492 262 1447
Fragments arranged by size:
1447 1353 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4469
SphI G_CATG'C
Cuts at: 1522 2022 1522
Size: 500 3969
StyI C'CwwG_G
Cuts at: 107 675 1214 1364 1603 1957 107
Size: 568 539 150 239 354 2619
Fragments arranged by size:
2619 568 539 354 239 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2052 2185 2644 2853 2052
Size: 133 459 209 3668
Fragments arranged by size:
3668 459 209 133
TatI w'GTAC_w
Cuts at: 602 925 3984 602
Size: 323 3059 1087
Fragments arranged by size:
3059 1087 323
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3986 4018 3986
Size: 32 4437
Tth111I GACn'n_nGTC
Cuts at: 1390 2266 1390
Size: 876 3593
XbaI T'CTAG_A
Cuts at: 1500 1500
Size: 4469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 371 653 1964 371
Size: 282 1311 2876
Fragments arranged by size:
2876 1311 282
XmaI C'CCGG_G
Cuts at: 135 1489 135
Size: 1354 3115
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI