Restriction map for
pF1KB6660
(Circular) (Six-base) MAPSORT of: pF1KB6660.seq Check: 2031 from: 1 to: 4466
pF1KB6660 4466 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 190 190
Size: 4466
AccI GT'mk_AC
Cuts at: 1504 1504
Size: 4466
Acc65I G'GTAC_C
Cuts at: 1482 1482
Size: 4466
AclI AA'CG_TT
Cuts at: 4306 4306
Size: 4466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 2291 2723 3470 3738 320
Size: 1971 432 747 268 1048
Fragments arranged by size:
1971 1048 747 432 268
AflIII A'CryG_T
Cuts at: 1818 2922 3899 1818
Size: 1104 977 2385
Fragments arranged by size:
2385 1104 977
AgeI A'CCGG_T
Cuts at: 2854 2854
Size: 4466
AhdI GACnn_n'nnGTC
Cuts at: 313 1035 313
Size: 722 3744
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 692 692
Size: 4466
AlwNI CAG_nnn'CTG
Cuts at: 1771 3338 1771
Size: 1567 2899
ApaI G_GGCC'C
Cuts at: 580 580
Size: 4466
ApaLI G'TGCA_C
Cuts at: 3236 3236
Size: 4466
ApoI r'AATT_y
Cuts at: 457 1177 1285 1453 1470 3819 4075 457
Size: 720 108 168 17 2349 256 848
Fragments arranged by size:
2349 848 720 256 168 108 17
AseI AT'TA_AT
Cuts at: 20 367 20
Size: 347 4119
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 1486 1486
Size: 4466
BamHI G'GATC_C
Cuts at: 1491 1491
Size: 4466
BanI G'GyrC_C
Cuts at: 1482 1896 2144 2179 1482
Size: 414 248 35 3769
Fragments arranged by size:
3769 414 248 35
BanII G_rGCy'C
Cuts at: 580 1480 2510 580
Size: 900 1030 2536
Fragments arranged by size:
2536 1030 900
BbeI G_GCGC'C
Cuts at: 1900 2148 1900
Size: 248 4218
BbsI GAAGACnn'nnnn_
Cuts at: 347 403 1034 347
Size: 56 631 3779
Fragments arranged by size:
3779 631 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4432
BciVI GTATCCnnnnn_n'
Cuts at: 2364 3125 4466 2364
Size: 761 1341 2364
Fragments arranged by size:
2364 1341 761
BclI T'GATC_A
Cuts at: 1986 1986
Size: 4466
BfrBI ATG'CAT
Cuts at: 244 906 244
Size: 662 3804
BglII A'GATC_T
Cuts at: 1981 4018 1981
Size: 2037 2429
BlpI GC'TnA_GC
Cuts at: 1578 1578
Size: 4466
Bme1580I G_kGCm'C
Cuts at: 580 2091 2184 3240 580
Size: 1511 93 1056 1806
Fragments arranged by size:
1806 1511 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1312 1678 1759 2094 4238 1312
Size: 366 81 335 2144 1540
Fragments arranged by size:
2144 1540 366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4466
Bpu10I CC'TnA_GC
Cuts at: 1054 1054
Size: 4466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 919 1642 2521 3013 3311 320
Size: 599 723 879 492 298 1475
Fragments arranged by size:
1475 879 723 599 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4170 4170
Size: 4466
BsaAI yAC'GTr
Cuts at: 1819 1819
Size: 4466
BsaBI GATnn'nnATC
Cuts at: 6 1980 4017 6
Size: 1974 2037 455
Fragments arranged by size:
2037 1974 455
BsaHI Gr'CG_yC
Cuts at: 1897 2145 2847 4343 1897
Size: 248 702 1496 2020
Fragments arranged by size:
2020 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1655 2176 2854 3128 3275 3686 1655
Size: 521 678 274 147 411 2435
Fragments arranged by size:
2435 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 919 919
Size: 4466
BseYI C'CCAG_C
Cuts at: 573 1208 1891 2649 3226 3722 573
Size: 635 683 758 577 496 1317
Fragments arranged by size:
1317 758 683 635 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 557 557
Size: 4466
BsiEI CG_ry'CG
Cuts at: 104 2008 2054 3262 104
Size: 1904 46 1208 1308
Fragments arranged by size:
1904 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1480 2258 2448 3240 1480
Size: 778 190 792 2706
Fragments arranged by size:
2706 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 580 1480 2091 2184 2258 2448 2510 3240 580
Size: 900 611 93 74 190 62 730 1806
Fragments arranged by size:
1806 900 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3686 3686
Size: 4466
BspHI T'CATG_A
Cuts at: 3642 3642
Size: 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 190 1516 1730 2032 2413 190
Size: 1326 214 302 381 2243
Fragments arranged by size:
2243 1326 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2758 2812 4296 4461 50
Size: 2708 54 1484 165 55
Fragments arranged by size:
2708 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 264 2378 264
Size: 2114 2352
BsrFI r'CCGG_y
Cuts at: 2464 2645 2854 2464
Size: 181 209 4076
Fragments arranged by size:
4076 209 181
BssSI C'ACGA_G
Cuts at: 2737 3095 2737
Size: 358 4108
BstAPI GCAn_nnn'nTGC
Cuts at: 1843 1843
Size: 4466
BstBI TT'CG_AA
Cuts at: 1293 1367 2827 1293
Size: 74 1460 2932
Fragments arranged by size:
2932 1460 74
BstXI CCAn_nnnn'nTGG
Cuts at: 271 271
Size: 4466
BstYI r'GATC_y
Cuts at: 1 1491 1958 1981 2316 2562 3574 3660 3672
Size: 1490 467 23 335 246 1012 86 12
Cuts at: 3672 4018 1
Size: 346 449
Fragments arranged by size:
1490 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1135 1135
Size: 4466
BtgI C'CryG_G
Cuts at: 141 141
Size: 4466
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2416 2597 2760 2416
Size: 181 163 4122
Fragments arranged by size:
4122 181 163
BtsI GCAGTG_nn'
Cuts at: 156 276 1148 1709 156
Size: 120 872 561 2913
Fragments arranged by size:
2913 872 561 120
DraI TTT'AAA
Cuts at: 1465 1465
Size: 4466
DrdI GACnn_nn'nnGTC
Cuts at: 2172 3030 2172
Size: 858 3608
EaeI y'GGCC_r
Cuts at: 204 995 2051 2225 2616 2643 3988 204
Size: 791 1056 174 391 27 1345 682
Fragments arranged by size:
1345 1056 791 682 391 174 27
EagI C'GGCC_G
Cuts at: 2051 2051
Size: 4466
EarI CTCTTCn'nnn_
Cuts at: 590 845 2489 2699 590
Size: 255 1644 210 2357
Fragments arranged by size:
2357 1644 255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2674 2984 3130 4276 2674
Size: 310 146 1146 2864
Fragments arranged by size:
2864 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1478 1478
Size: 4466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 2291 2723 3470 3738 320
Size: 1971 432 747 268 1048
Fragments arranged by size:
1971 1048 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 322 322
Size: 4466
EcoO109I rG'GnC_Cy
Cuts at: 577 1058 1605 577
Size: 481 547 3438
Fragments arranged by size:
3438 547 481
EcoRI G'AATT_C
Cuts at: 1285 1453 1470 1285
Size: 168 17 4281
Fragments arranged by size:
4281 168 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 430 2485 430
Size: 2055 2411
FspI TGC'GCA
Cuts at: 2247 2247
Size: 4466
HaeII r_GCGC'y
Cuts at: 1900 2148 3170 4149 1900
Size: 248 1022 979 2217
Fragments arranged by size:
2217 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 299 331 1021 1053 2308 2338 2340 2370 299
Size: 32 690 32 1255 30 2 30 2395
Fragments arranged by size:
2395 1255 690 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1505 1505
Size: 4466
HindIII A'AGCT_T
Cuts at: 895 916 895
Size: 21 4445
KasI G'GCGC_C
Cuts at: 1896 2144 1896
Size: 248 4218
KpnI G_GTAC'C
Cuts at: 1486 1486
Size: 4466
MfeI C'AATT_G
Cuts at: 248 248
Size: 4466
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1186 1428 3137 3321 1186
Size: 242 1709 184 2331
Fragments arranged by size:
2331 1709 242 184
MscI TGG'CCA
Cuts at: 206 997 2227 206
Size: 791 1230 2445
Fragments arranged by size:
2445 1230 791
MslI CAynn'nnrTG
Cuts at: 215 269 1226 2455 2582 215
Size: 54 957 1229 127 2099
Fragments arranged by size:
2099 1229 957 127 54
MspA1I CmG'CkG
Cuts at: 143 541 573 1113 1577 1891 2251 3264 3509
Size: 398 32 540 464 314 360 1013 245
Cuts at: 3509 143
Size: 1100
Fragments arranged by size:
1100 1013 540 464 398 360 314 245
32
NaeI GCC'GGC
Cuts at: 2647 2647
Size: 4466
NarI GG'CG_CC
Cuts at: 1897 2145 1897
Size: 248 4218
NdeI CA'TA_TG
Cuts at: 868 868
Size: 4466
NgoMIV G'CCGG_C
Cuts at: 2645 2645
Size: 4466
NsiI A_TGCA'T
Cuts at: 246 908 246
Size: 662 3804
NspI r_CATG'y
Cuts at: 1197 1519 2019 2926 1197
Size: 322 500 907 2737
Fragments arranged by size:
2737 907 500 322
PciI A'CATG_T
Cuts at: 2922 2922
Size: 4466
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4434
PspOMI G'GGCC_C
Cuts at: 576 576
Size: 4466
PstI C_TGCA'G
Cuts at: 546 1392 1513 2198 546
Size: 846 121 685 2814
Fragments arranged by size:
2814 846 685 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 541 573 1891 2251 541
Size: 32 1318 360 2756
Fragments arranged by size:
2756 1318 360 32
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 1480 1480
Size: 4466
SacII CC_GC'GG
Cuts at: 144 144
Size: 4466
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SapI GCTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SfcI C'TryA_G
Cuts at: 33 542 1388 1509 2194 3187 3378 33
Size: 509 846 121 685 993 191 1121
Fragments arranged by size:
1121 993 846 685 509 191 121
SfoI GGC'GCC
Cuts at: 1898 2146 1898
Size: 248 4218
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 1488 1488
Size: 4466
SmlI C'TyrA_G
Cuts at: 335 898 1621 2536 3028 3290 335
Size: 563 723 915 492 262 1511
Fragments arranged by size:
1511 915 723 563 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1519 2019 1519
Size: 500 3966
StyI C'CwwG_G
Cuts at: 1061 1600 1954 1061
Size: 539 354 3573
Fragments arranged by size:
3573 539 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2049 2182 2641 2850 2049
Size: 133 459 209 3665
Fragments arranged by size:
3665 459 209 133
TatI w'GTAC_w
Cuts at: 350 3981 350
Size: 3631 835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1304 1336 3983 4015 1304
Size: 32 2647 32 1755
Fragments arranged by size:
2647 1755 32 32
Tth111I GACn'n_nGTC
Cuts at: 2263 2263
Size: 4466
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1961 1961
Size: 4466
XmaI C'CCGG_G
Cuts at: 1486 1486
Size: 4466
XmnI GAAnn'nnTTC
Cuts at: 461 1289 461
Size: 828 3638
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BbvCI BglI BmgBI BmtI BpmI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI NcoI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI