Restriction map for
pF1KB6658
(Circular) (Six-base) MAPSORT of: pF1KB6658.seq Check: 6407 from: 1 to: 4466
pF1KB6658 4466 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 981 981
Size: 4466
AatII G_ACGT'C
Cuts at: 209 1130 1217 209
Size: 921 87 3458
Fragments arranged by size:
3458 921 87
AccI GT'mk_AC
Cuts at: 1130 1504 1130
Size: 374 4092
Acc65I G'GTAC_C
Cuts at: 1482 1482
Size: 4466
AclI AA'CG_TT
Cuts at: 4306 4306
Size: 4466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1196 2291 2723 3470 3738 1196
Size: 1095 432 747 268 1924
Fragments arranged by size:
1924 1095 747 432 268
AfeI AGC'GCT
Cuts at: 1181 1181
Size: 4466
AflIII A'CryG_T
Cuts at: 1818 2922 3899 1818
Size: 1104 977 2385
Fragments arranged by size:
2385 1104 977
AgeI A'CCGG_T
Cuts at: 2854 2854
Size: 4466
AhdI GACnn_n'nnGTC
Cuts at: 165 1218 165
Size: 1053 3413
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 325 357 325
Size: 32 4434
AlwNI CAG_nnn'CTG
Cuts at: 585 801 1771 3338 585
Size: 216 970 1567 1713
Fragments arranged by size:
1713 1567 970 216
ApaI G_GGCC'C
Cuts at: 139 579 139
Size: 440 4026
ApaLI G'TGCA_C
Cuts at: 1354 3236 1354
Size: 1882 2584
ApoI r'AATT_y
Cuts at: 1470 3819 4075 1470
Size: 2349 256 1861
Fragments arranged by size:
2349 1861 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 116 553 773 1187 1390 1486 116
Size: 437 220 414 203 96 3096
Fragments arranged by size:
3096 437 414 220 203 96
BamHI G'GATC_C
Cuts at: 1491 1491
Size: 4466
BanI G'GyrC_C
Cuts at: 406 1263 1482 1896 2144 2179 406
Size: 857 219 414 248 35 2693
Fragments arranged by size:
2693 857 414 248 219 35
BanII G_rGCy'C
Cuts at: 139 579 638 734 905 1480 2510 139
Size: 440 59 96 171 575 1030 2095
Fragments arranged by size:
2095 1030 575 440 171 96 59
BbeI G_GCGC'C
Cuts at: 410 1900 2148 410
Size: 1490 248 2728
Fragments arranged by size:
2728 1490 248
BbsI GAAGACnn'nnnn_
Cuts at: 201 714 201
Size: 513 3953
BbvCI CC'TCA_GC
Cuts at: 544 998 544
Size: 454 4012
BciVI GTATCCnnnnn_n'
Cuts at: 2364 3125 4466 2364
Size: 761 1341 2364
Fragments arranged by size:
2364 1341 761
BclI T'GATC_A
Cuts at: 416 1986 416
Size: 1570 2896
BglII A'GATC_T
Cuts at: 491 1981 4018 491
Size: 1490 2037 939
Fragments arranged by size:
2037 1490 939
BlpI GC'TnA_GC
Cuts at: 187 1578 187
Size: 1391 3075
Bme1580I G_kGCm'C
Cuts at: 139 296 579 1268 1345 1358 2091 2184 3240
Size: 157 283 689 77 13 733 93 1056
Cuts at: 3240 139
Size: 1365
Fragments arranged by size:
1365 1056 733 689 283 157 93 77
13
BmrI ACTGGGnnnn_n'
Cuts at: 666 1678 1759 2094 4238 666
Size: 1012 81 335 2144 894
Fragments arranged by size:
2144 1012 894 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 799 1446 2833 799
Size: 647 1387 2432
Fragments arranged by size:
2432 1387 647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1036 1357 1416 946
Size: 90 321 59 3996
Fragments arranged by size:
3996 321 90 59
Bpu10I CC'TnA_GC
Cuts at: 544 632 998 544
Size: 88 366 4012
Fragments arranged by size:
4012 366 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 2521 3013 3311 1642
Size: 879 492 298 2797
Fragments arranged by size:
2797 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1392 4170 1392
Size: 2778 1688
BsaAI yAC'GTr
Cuts at: 216 1819 216
Size: 1603 2863
BsaBI GATnn'nnATC
Cuts at: 6 1980 4017 6
Size: 1974 2037 455
Fragments arranged by size:
2037 1974 455
BsaHI Gr'CG_yC
Cuts at: 206 407 1127 1214 1310 1897 2145 2847 4343
Size: 201 720 87 96 587 248 702 1496
Cuts at: 4343 206
Size: 329
Fragments arranged by size:
1496 720 702 587 329 248 201 96
87
BsaWI w'CCGG_w
Cuts at: 1655 2176 2854 3128 3275 3686 1655
Size: 521 678 274 147 411 2435
Fragments arranged by size:
2435 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 220 223 250 253 220
Size: 3 27 3 4433
Fragments arranged by size:
4433 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 244 688 196
Size: 48 444 3974
Fragments arranged by size:
3974 444 48
BseYI C'CCAG_C
Cuts at: 696 1348 1891 2649 3226 3722 696
Size: 652 543 758 577 496 1440
Fragments arranged by size:
1440 758 652 577 543 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 772 1401 502
Size: 270 629 3567
Fragments arranged by size:
3567 629 270
BsiEI CG_ry'CG
Cuts at: 104 1460 2008 2054 3262 104
Size: 1356 548 46 1208 1308
Fragments arranged by size:
1356 1308 1208 548 46
BsiHKAI G_wGCw'C
Cuts at: 905 1358 1480 2258 2448 3240 905
Size: 453 122 778 190 792 2131
Fragments arranged by size:
2131 792 778 453 190 122
BsmBI CGTCTCn'nnnn_
Cuts at: 313 313
Size: 4466
Bsp1286I G_dGCh'C
Cuts at: 139 296 579 638 734 905 1268 1345 1358
Size: 157 283 59 96 171 363 77 13
Cuts at: 1358 1480 2091 2184 2258 2448 2510 3240 139
Size: 122 611 93 74 190 62 730 1365
Fragments arranged by size:
1365 730 611 363 283 190 171 157
122 96 93 77 74 62 59 13
BspEI T'CCGG_A
Cuts at: 3686 3686
Size: 4466
BspHI T'CATG_A
Cuts at: 3642 3642
Size: 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 981 1516 1730 2032 2413 981
Size: 535 214 302 381 3034
Fragments arranged by size:
3034 535 381 302 214
BsrBI CCG'CTC
Cuts at: 50 362 2758 2812 4296 4461 50
Size: 312 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 312 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2378 2378
Size: 4466
BsrFI r'CCGG_y
Cuts at: 512 1312 2464 2645 2854 512
Size: 800 1152 181 209 2124
Fragments arranged by size:
2124 1152 800 209 181
BsrGI T'GTAC_A
Cuts at: 887 887
Size: 4466
BssSI C'ACGA_G
Cuts at: 625 2737 3095 625
Size: 2112 358 1996
Fragments arranged by size:
2112 1996 358
BstAPI GCAn_nnn'nTGC
Cuts at: 477 801 1328 1843 477
Size: 324 527 515 3100
Fragments arranged by size:
3100 527 515 324
BstBI TT'CG_AA
Cuts at: 2827 2827
Size: 4466
BstXI CCAn_nnnn'nTGG
Cuts at: 147 255 147
Size: 108 4358
BstYI r'GATC_y
Cuts at: 1 491 812 1491 1958 1981 2316 2562 3574
Size: 490 321 679 467 23 335 246 1012
Cuts at: 3574 3660 3672 4018 1
Size: 86 12 346 449
Fragments arranged by size:
1012 679 490 467 449 346 335 321
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1300 1300
Size: 4466
BtgI C'CryG_G
Cuts at: 107 275 337 401 591 863 872 107
Size: 168 62 64 190 272 9 3701
Fragments arranged by size:
3701 272 190 168 64 62 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 966 2416 2597 2760 966
Size: 1450 181 163 2672
Fragments arranged by size:
2672 1450 181 163
BtsI GCAGTG_nn'
Cuts at: 241 1709 241
Size: 1468 2998
DraI TTT'AAA
Cuts at: 1465 1465
Size: 4466
DrdI GACnn_nn'nnGTC
Cuts at: 327 2172 3030 327
Size: 1845 858 1763
Fragments arranged by size:
1845 1763 858
EaeI y'GGCC_r
Cuts at: 218 308 605 755 908 1046 2051 2225 2616
Size: 90 297 150 153 138 1005 174 391
Cuts at: 2616 2643 3988 218
Size: 27 1345 696
Fragments arranged by size:
1345 1005 696 391 297 174 153 150
138 90 27
EagI C'GGCC_G
Cuts at: 2051 2051
Size: 4466
EarI CTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2674 2984 3130 4276 2674
Size: 310 146 1146 2864
Fragments arranged by size:
2864 1146 310 146
EcoICRI GAG'CTC
Cuts at: 903 1478 903
Size: 575 3891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1036 1196 1357 1416 2291 2723 3470 3738
Size: 90 160 161 59 875 432 747 268
Cuts at: 3738 946
Size: 1674
Fragments arranged by size:
1674 875 747 432 268 161 160 90
59
EcoNI CCTnn'n_nnAGG
Cuts at: 526 1299 526
Size: 773 3693
EcoO109I rG'GnC_Cy
Cuts at: 136 575 879 1041 1386 1413 1605 136
Size: 439 304 162 345 27 192 2997
Fragments arranged by size:
2997 439 345 304 192 162 27
EcoRI G'AATT_C
Cuts at: 1470 1470
Size: 4466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4466
FspI TGC'GCA
Cuts at: 2247 2247
Size: 4466
HaeII r_GCGC'y
Cuts at: 410 856 1183 1900 2148 3170 4149 410
Size: 446 327 717 248 1022 979 727
Fragments arranged by size:
1022 979 727 717 446 327 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 520 552 1204 1236 2308 2338 2340
Size: 32 337 32 652 32 1072 30 2
Cuts at: 2340 2370 151
Size: 30 2247
Fragments arranged by size:
2247 1072 652 337 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1505 1505
Size: 4466
KasI G'GCGC_C
Cuts at: 406 1896 2144 406
Size: 1490 248 2728
Fragments arranged by size:
2728 1490 248
KpnI G_GTAC'C
Cuts at: 1486 1486
Size: 4466
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 788 1235 1307 3137 3321 311
Size: 477 447 72 1830 184 1456
Fragments arranged by size:
1830 1456 477 447 184 72
MscI TGG'CCA
Cuts at: 220 757 910 2227 220
Size: 537 153 1317 2459
Fragments arranged by size:
2459 1317 537 153
MslI CAynn'nnrTG
Cuts at: 650 763 1377 2455 2582 650
Size: 113 614 1078 127 2534
Fragments arranged by size:
2534 1078 614 127 113
MspA1I CmG'CkG
Cuts at: 1577 1891 2251 3264 3509 1577
Size: 314 360 1013 245 2534
Fragments arranged by size:
2534 1013 360 314 245
NaeI GCC'GGC
Cuts at: 514 1314 2647 514
Size: 800 1333 2333
Fragments arranged by size:
2333 1333 800
NarI GG'CG_CC
Cuts at: 407 1897 2145 407
Size: 1490 248 2728
Fragments arranged by size:
2728 1490 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4466
NgoMIV G'CCGG_C
Cuts at: 512 1312 2645 512
Size: 800 1333 2333
Fragments arranged by size:
2333 1333 800
NspI r_CATG'y
Cuts at: 1008 1281 1326 1376 1519 2019 2926 1008
Size: 273 45 50 143 500 907 2548
Fragments arranged by size:
2548 907 500 273 143 50 45
PasI CC'CwG_GG
Cuts at: 282 282
Size: 4466
PciI A'CATG_T
Cuts at: 2922 2922
Size: 4466
PfoI T'CCnGG_A
Cuts at: 169 169
Size: 4466
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PmlI CAC'GTG
Cuts at: 216 216
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4434
PpuMI rG'GwC_Cy
Cuts at: 879 1041 1386 879
Size: 162 345 3959
Fragments arranged by size:
3959 345 162
PspOMI G'GGCC_C
Cuts at: 135 575 135
Size: 440 4026
PstI C_TGCA'G
Cuts at: 386 691 806 1513 2198 386
Size: 305 115 707 685 2654
Fragments arranged by size:
2654 707 685 305 115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 1891 2251 1891
Size: 360 4106
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 905 1480 905
Size: 575 3891
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SanDI GG'GwC_CC
Cuts at: 879 1386 879
Size: 507 3959
SapI GCTCTTCn'nnn_
Cuts at: 2489 2699 2489
Size: 210 4256
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SfcI C'TryA_G
Cuts at: 33 382 687 802 1509 2194 3187 3378 33
Size: 349 305 115 707 685 993 191 1121
Fragments arranged by size:
1121 993 707 685 349 305 191 115
SfoI GGC'GCC
Cuts at: 408 1898 2146 408
Size: 1490 248 2728
Fragments arranged by size:
2728 1490 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 118 1488 118
Size: 1370 3096
SmlI C'TyrA_G
Cuts at: 773 1621 2536 3028 3290 773
Size: 848 915 492 262 1949
Fragments arranged by size:
1949 915 848 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1326 1519 2019 1326
Size: 193 500 3773
Fragments arranged by size:
3773 500 193
StuI AGG'CCT
Cuts at: 1294 1294
Size: 4466
StyI C'CwwG_G
Cuts at: 107 911 1600 1954 107
Size: 804 689 354 2619
Fragments arranged by size:
2619 804 689 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2049 2182 2641 2850 2049
Size: 133 459 209 3665
Fragments arranged by size:
3665 459 209 133
TatI w'GTAC_w
Cuts at: 887 3981 887
Size: 3094 1372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 220 223 252 255 3983 4015 220
Size: 3 29 3 3728 32 671
Fragments arranged by size:
3728 671 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 448 745 2263 448
Size: 297 1518 2651
Fragments arranged by size:
2651 1518 297
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1961 1961
Size: 4466
XhoI C'TCGA_G
Cuts at: 773 773
Size: 4466
XmaI C'CCGG_G
Cuts at: 116 1486 116
Size: 1370 3096
ZraI GAC'GTC
Cuts at: 207 1128 1215 207
Size: 921 87 3458
Fragments arranged by size:
3458 921 87
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AleI AlfI AscI AvrII BaeI BcgI BfrBI
BglI BmgBI BmtI BsiWI BsmI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI