Restriction map for
pF1KB6656
(Circular) (Six-base) MAPSORT of: pF1KB6656.seq Check: 1473 from: 1 to: 4463
pF1KB6656 4463 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 1292 1292
Size: 4463
AccI GT'mk_AC
Cuts at: 1501 1501
Size: 4463
Acc65I G'GTAC_C
Cuts at: 328 1479 328
Size: 1151 3312
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 833 1303 2288 2720 3467 3735 712
Size: 121 470 985 432 747 268 1440
Fragments arranged by size:
1440 985 747 470 432 268 121
AflIII A'CryG_T
Cuts at: 1211 1815 2919 3896 1211
Size: 604 1104 977 1778
Fragments arranged by size:
1778 1104 977 604
AgeI A'CCGG_T
Cuts at: 2851 2851
Size: 4463
AleI CACnn'nnGTG
Cuts at: 220 220
Size: 4463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 868 868
Size: 4463
AlwNI CAG_nnn'CTG
Cuts at: 189 668 861 1171 1768 3335 189
Size: 479 193 310 597 1567 1317
Fragments arranged by size:
1567 1317 597 479 310 193
ApaI G_GGCC'C
Cuts at: 675 675
Size: 4463
ApaLI G'TGCA_C
Cuts at: 3233 3233
Size: 4463
ApoI r'AATT_y
Cuts at: 1192 1274 1467 3816 4072 1192
Size: 82 193 2349 256 1583
Fragments arranged by size:
2349 1583 256 193 82
AseI AT'TA_AT
Cuts at: 20 20
Size: 4463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 1298 1483 1298
Size: 185 4278
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 328 1376 1479 1893 2141 2176 328
Size: 1048 103 414 248 35 2615
Fragments arranged by size:
2615 1048 414 248 103 35
BanII G_rGCy'C
Cuts at: 675 893 1477 2507 675
Size: 218 584 1030 2631
Fragments arranged by size:
2631 1030 584 218
BbeI G_GCGC'C
Cuts at: 1897 2145 1897
Size: 248 4215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 775 809 1017 1051 775
Size: 34 208 34 4187
Fragments arranged by size:
4187 208 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3122 4463 2361
Size: 761 1341 2361
Fragments arranged by size:
2361 1341 761
BclI T'GATC_A
Cuts at: 1983 1983
Size: 4463
BglI GCCn_nnn'nGGC
Cuts at: 278 808 1174 278
Size: 530 366 3567
Fragments arranged by size:
3567 530 366
BglII A'GATC_T
Cuts at: 1978 4015 1978
Size: 2037 2426
BlpI GC'TnA_GC
Cuts at: 383 520 1575 383
Size: 137 1055 3271
Fragments arranged by size:
3271 1055 137
Bme1580I G_kGCm'C
Cuts at: 634 675 2088 2181 3237 634
Size: 41 1413 93 1056 1860
Fragments arranged by size:
1860 1413 1056 93 41
BmgBI CAC'GTC
Cuts at: 210 732 1212 210
Size: 522 480 3461
Fragments arranged by size:
3461 522 480
BmrI ACTGGGnnnn_n'
Cuts at: 1675 1756 2091 4235 1675
Size: 81 335 2144 1903
Fragments arranged by size:
2144 1903 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 229 994 2830 229
Size: 765 1836 1862
Fragments arranged by size:
1862 1836 765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1042
Size: 4463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1447 1639 2518 3010 3308 967
Size: 480 192 879 492 298 2122
Fragments arranged by size:
2122 879 492 480 298 192
BsaI GGTCTCn'nnnn_
Cuts at: 1040 1114 4167 1040
Size: 74 3053 1336
Fragments arranged by size:
3053 1336 74
BsaAI yAC'GTr
Cuts at: 1816 1816
Size: 4463
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 788 1123 1289 1385 1894 2142 2844 4340 788
Size: 335 166 96 509 248 702 1496 911
Fragments arranged by size:
1496 911 702 509 335 248 166 96
BsaWI w'CCGG_w
Cuts at: 136 1652 2173 2851 3125 3272 3683 136
Size: 1516 521 678 274 147 411 916
Fragments arranged by size:
1516 916 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 892 970 547
Size: 345 78 4040
Fragments arranged by size:
4040 345 78
BseYI C'CCAG_C
Cuts at: 288 668 738 963 1888 2646 3223 3719 288
Size: 380 70 225 925 758 577 496 1032
Fragments arranged by size:
1032 925 758 577 496 380 225 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1435 874
Size: 561 3902
BsiEI CG_ry'CG
Cuts at: 104 2005 2051 3259 104
Size: 1901 46 1208 1308
Fragments arranged by size:
1901 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 215 436 491 572 1433 1477 2255 2445 3237
Size: 221 55 81 861 44 778 190 792
Cuts at: 3237 215
Size: 1441
Fragments arranged by size:
1441 861 792 778 221 190 81 55
44
BsmBI CGTCTCn'nnnn_
Cuts at: 200 722 200
Size: 522 3941
Bsp1286I G_dGCh'C
Cuts at: 215 436 491 572 634 675 893 1433 1477
Size: 221 55 81 62 41 218 540 44
Cuts at: 1477 2088 2181 2255 2445 2507 3237 215
Size: 611 93 74 190 62 730 1441
Fragments arranged by size:
1441 730 611 540 221 218 190 93
81 74 62 62 55 44 41
BspEI T'CCGG_A
Cuts at: 3683 3683
Size: 4463
BspHI T'CATG_A
Cuts at: 824 1361 3639 824
Size: 537 2278 1648
Fragments arranged by size:
2278 1648 537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 947 1513 1727 2029 2410 197
Size: 750 566 214 302 381 2250
Fragments arranged by size:
2250 750 566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 387 2755 2809 4293 4458 50
Size: 337 2368 54 1484 165 55
Fragments arranged by size:
2368 1484 337 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1068 2375 1068
Size: 1307 3156
BsrFI r'CCGG_y
Cuts at: 2461 2642 2851 2461
Size: 181 209 4073
Fragments arranged by size:
4073 209 181
BssSI C'ACGA_G
Cuts at: 1444 2734 3092 1444
Size: 1290 358 2815
Fragments arranged by size:
2815 1290 358
BstAPI GCAn_nnn'nTGC
Cuts at: 189 1840 189
Size: 1651 2812
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstXI CCAn_nnnn'nTGG
Cuts at: 1160 1160
Size: 4463
BstYI r'GATC_y
Cuts at: 1 1488 1955 1978 2313 2559 3571 3657 3669
Size: 1487 467 23 335 246 1012 86 12
Cuts at: 3669 4015 1
Size: 346 449
Fragments arranged by size:
1487 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1172 1172
Size: 4463
BtgI C'CryG_G
Cuts at: 168 357 1010 168
Size: 189 653 3621
Fragments arranged by size:
3621 653 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2413 2594 2757 2413
Size: 181 163 4119
Fragments arranged by size:
4119 181 163
BtsI GCAGTG_nn'
Cuts at: 173 791 1706 173
Size: 618 915 2930
Fragments arranged by size:
2930 915 618
DraI TTT'AAA
Cuts at: 1462 1462
Size: 4463
DraIII CAC_nnn'GTG
Cuts at: 461 461
Size: 4463
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3027 2169
Size: 858 3605
EaeI y'GGCC_r
Cuts at: 130 224 914 1088 2048 2222 2613 2640 3985
Size: 94 690 174 960 174 391 27 1345
Cuts at: 3985 130
Size: 608
Fragments arranged by size:
1345 960 690 608 391 174 174 94
27
EagI C'GGCC_G
Cuts at: 2048 2048
Size: 4463
EarI CTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 983 2671 2981 3127 4273 983
Size: 1688 310 146 1146 1173
Fragments arranged by size:
1688 1173 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1475 1475
Size: 4463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 833 1042 1303 2288 2720 3467 3735 712
Size: 121 209 261 985 432 747 268 1440
Fragments arranged by size:
1440 985 747 432 268 261 209 121
EcoO109I rG'GnC_Cy
Cuts at: 186 237 672 1176 1602 186
Size: 51 435 504 426 3047
Fragments arranged by size:
3047 504 435 426 51
EcoRI G'AATT_C
Cuts at: 1274 1467 1274
Size: 193 4270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 180 2482 180
Size: 2302 2161
FspI TGC'GCA
Cuts at: 2244 2244
Size: 4463
HaeII r_GCGC'y
Cuts at: 1897 2145 3167 4146 1897
Size: 248 1022 979 2214
Fragments arranged by size:
2214 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2305 2335 2337 2367 2305
Size: 30 2 30 4401
Fragments arranged by size:
4401 30 30 2
HincII GTy'rAC
Cuts at: 1502 1502
Size: 4463
KasI G'GCGC_C
Cuts at: 1893 2141 1893
Size: 248 4215
KpnI G_GTAC'C
Cuts at: 332 1483 332
Size: 1151 3312
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3134 3318 3134
Size: 184 4279
MscI TGG'CCA
Cuts at: 132 916 1090 2224 132
Size: 784 174 1134 2371
Fragments arranged by size:
2371 1134 784 174
MslI CAynn'nnrTG
Cuts at: 220 2452 2579 220
Size: 2232 127 2104
Fragments arranged by size:
2232 2104 127
MspA1I CmG'CkG
Cuts at: 561 665 1574 1888 2248 3261 3506 561
Size: 104 909 314 360 1013 245 1518
Fragments arranged by size:
1518 1013 909 360 314 245 104
NaeI GCC'GGC
Cuts at: 2644 2644
Size: 4463
NarI GG'CG_CC
Cuts at: 1894 2142 1894
Size: 248 4215
NcoI C'CATG_G
Cuts at: 1010 1010
Size: 4463
NgoMIV G'CCGG_C
Cuts at: 2642 2642
Size: 4463
NspI r_CATG'y
Cuts at: 1516 2016 2923 1516
Size: 500 907 3056
Fragments arranged by size:
3056 907 500
PciI A'CATG_T
Cuts at: 2919 2919
Size: 4463
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4431
PpuMI rG'GwC_Cy
Cuts at: 186 237 186
Size: 51 4412
PshAI GACnn'nnGTC
Cuts at: 1106 1106
Size: 4463
PspOMI G'GGCC_C
Cuts at: 671 671
Size: 4463
PstI C_TGCA'G
Cuts at: 704 1510 2195 704
Size: 806 685 2972
Fragments arranged by size:
2972 806 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 561 1888 2248 561
Size: 1327 360 2776
Fragments arranged by size:
2776 1327 360
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 1477 1477
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SexAI A'CCwGG_T
Cuts at: 848 1454 848
Size: 606 3857
SfcI C'TryA_G
Cuts at: 33 700 1408 1506 2191 3184 3375 33
Size: 667 708 98 685 993 191 1121
Fragments arranged by size:
1121 993 708 685 667 191 98
SfoI GGC'GCC
Cuts at: 1895 2143 1895
Size: 248 4215
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 1485 1485
Size: 4463
SmlI C'TyrA_G
Cuts at: 982 1426 1618 2533 3025 3287 982
Size: 444 192 915 492 262 2158
Fragments arranged by size:
2158 915 492 444 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 1516 2016 1516
Size: 500 3963
StuI AGG'CCT
Cuts at: 413 802 413
Size: 389 4074
StyI C'CwwG_G
Cuts at: 233 349 917 1010 1091 1115 1597 1951 233
Size: 116 568 93 81 24 482 354 2745
Fragments arranged by size:
2745 568 482 354 116 93 81 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 944 1147 2046 2179 2638 2847 944
Size: 203 899 133 459 209 2560
Fragments arranged by size:
2560 899 459 209 203 133
TatI w'GTAC_w
Cuts at: 158 539 908 3978 158
Size: 381 369 3070 643
Fragments arranged by size:
3070 643 381 369
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3980 4012 3980
Size: 32 4431
Tth111I GACn'n_nGTC
Cuts at: 1291 2260 1291
Size: 969 3494
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 264 1017 1958 264
Size: 753 941 2769
Fragments arranged by size:
2769 941 753
XmaI C'CCGG_G
Cuts at: 1483 1483
Size: 4463
XmnI GAAnn'nnTTC
Cuts at: 1192 1192
Size: 4463
ZraI GAC'GTC
Cuts at: 1290 1290
Size: 4463
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI