Restriction map for
pF1KB6651
(Circular) (Six-base) MAPSORT of: pF1KB6651.seq Check: 8238 from: 1 to: 4373
pF1KB6651 4373 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1411 1411
Size: 4373
Acc65I G'GTAC_C
Cuts at: 1389 1389
Size: 4373
AclI AA'CG_TT
Cuts at: 4213 4213
Size: 4373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 2198 2630 3377 3645 665
Size: 1533 432 747 268 1393
Fragments arranged by size:
1533 1393 747 432 268
AflIII A'CryG_T
Cuts at: 1725 2829 3806 1725
Size: 1104 977 2292
Fragments arranged by size:
2292 1104 977
AgeI A'CCGG_T
Cuts at: 2761 2761
Size: 4373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1176 1176
Size: 4373
AlwNI CAG_nnn'CTG
Cuts at: 443 895 1678 3245 443
Size: 452 783 1567 1571
Fragments arranged by size:
1571 1567 783 452
ApaLI G'TGCA_C
Cuts at: 3143 3143
Size: 4373
ApoI r'AATT_y
Cuts at: 498 1377 3726 3982 498
Size: 879 2349 256 889
Fragments arranged by size:
2349 889 879 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4373
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
AvaI C'yCGr_G
Cuts at: 1393 1393
Size: 4373
BamHI G'GATC_C
Cuts at: 1398 1398
Size: 4373
BanI G'GyrC_C
Cuts at: 1389 1803 2051 2086 1389
Size: 414 248 35 3676
Fragments arranged by size:
3676 414 248 35
BanII G_rGCy'C
Cuts at: 1048 1070 1387 2417 1048
Size: 22 317 1030 3004
Fragments arranged by size:
3004 1030 317 22
BbeI G_GCGC'C
Cuts at: 1807 2055 1807
Size: 248 4125
BbsI GAAGACnn'nnnn_
Cuts at: 533 533
Size: 4373
BbvCI CC'TCA_GC
Cuts at: 718 718
Size: 4373
BciVI GTATCCnnnnn_n'
Cuts at: 2271 3032 4373 2271
Size: 761 1341 2271
Fragments arranged by size:
2271 1341 761
BclI T'GATC_A
Cuts at: 110 270 789 816 1893 110
Size: 160 519 27 1077 2590
Fragments arranged by size:
2590 1077 519 160 27
BfrBI ATG'CAT
Cuts at: 985 985
Size: 4373
BglII A'GATC_T
Cuts at: 1257 1888 3925 1257
Size: 631 2037 1705
Fragments arranged by size:
2037 1705 631
BlpI GC'TnA_GC
Cuts at: 1485 1485
Size: 4373
Bme1580I G_kGCm'C
Cuts at: 1998 2091 3147 1998
Size: 93 1056 3224
Fragments arranged by size:
3224 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 685 1585 1666 2001 4145 685
Size: 900 81 335 2144 913
Fragments arranged by size:
2144 913 900 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 1092 1358 1372 744
Size: 348 266 14 3745
Fragments arranged by size:
3745 348 266 14
Bpu10I CC'TnA_GC
Cuts at: 718 718
Size: 4373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 1143 1549 2428 2920 3218 224
Size: 919 406 879 492 298 1379
Fragments arranged by size:
1379 919 879 492 406 298
BsaI GGTCTCn'nnnn_
Cuts at: 482 1339 4077 482
Size: 857 2738 778
Fragments arranged by size:
2738 857 778
BsaAI yAC'GTr
Cuts at: 1726 1726
Size: 4373
BsaBI GATnn'nnATC
Cuts at: 6 1887 3924 6
Size: 1881 2037 455
Fragments arranged by size:
2037 1881 455
BsaHI Gr'CG_yC
Cuts at: 1804 2052 2754 4250 1804
Size: 248 702 1496 1927
Fragments arranged by size:
1927 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1562 2083 2761 3035 3182 3593 1562
Size: 521 678 274 147 411 2342
Fragments arranged by size:
2342 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 443 1288 1798 2556 3133 3629 443
Size: 845 510 758 577 496 1187
Fragments arranged by size:
1187 845 758 577 510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 1074 555
Size: 519 3854
BsiEI CG_ry'CG
Cuts at: 104 1915 1961 3169 104
Size: 1811 46 1208 1308
Fragments arranged by size:
1811 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1048 1387 2165 2355 3147 1048
Size: 339 778 190 792 2274
Fragments arranged by size:
2274 792 778 339 190
BsmI GAATG_Cn'
Cuts at: 655 985 655
Size: 330 4043
BsmBI CGTCTCn'nnnn_
Cuts at: 871 1294 871
Size: 423 3950
Bsp1286I G_dGCh'C
Cuts at: 1048 1070 1387 1998 2091 2165 2355 2417 3147
Size: 22 317 611 93 74 190 62 730
Cuts at: 3147 1048
Size: 2274
Fragments arranged by size:
2274 730 611 317 190 93 74 62
22
BspEI T'CCGG_A
Cuts at: 3593 3593
Size: 4373
BspHI T'CATG_A
Cuts at: 3549 3549
Size: 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1423 1637 1939 2320 1423
Size: 214 302 381 3476
Fragments arranged by size:
3476 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2665 2719 4203 4368 50
Size: 2615 54 1484 165 55
Fragments arranged by size:
2615 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 909 2285 909
Size: 1376 2997
BsrFI r'CCGG_y
Cuts at: 2371 2552 2761 2371
Size: 181 209 3983
Fragments arranged by size:
3983 209 181
BssSI C'ACGA_G
Cuts at: 2644 3002 2644
Size: 358 4015
BstAPI GCAn_nnn'nTGC
Cuts at: 1750 1750
Size: 4373
BstBI TT'CG_AA
Cuts at: 2734 2734
Size: 4373
BstYI r'GATC_y
Cuts at: 1 1257 1398 1865 1888 2223 2469 3481 3567
Size: 1256 141 467 23 335 246 1012 86
Cuts at: 3567 3579 3925 1
Size: 12 346 449
Fragments arranged by size:
1256 1012 467 449 346 335 246 141
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 599 599
Size: 4373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2323 2504 2667 2323
Size: 181 163 4029
Fragments arranged by size:
4029 181 163
BtsI GCAGTG_nn'
Cuts at: 567 1046 1616 567
Size: 479 570 3324
Fragments arranged by size:
3324 570 479
DraI TTT'AAA
Cuts at: 1372 1372
Size: 4373
DrdI GACnn_nn'nnGTC
Cuts at: 2079 2937 2079
Size: 858 3515
EaeI y'GGCC_r
Cuts at: 434 1958 2132 2523 2550 3895 434
Size: 1524 174 391 27 1345 912
Fragments arranged by size:
1524 1345 912 391 174 27
EagI C'GGCC_G
Cuts at: 1958 1958
Size: 4373
EarI CTCTTCn'nnn_
Cuts at: 2396 2606 2396
Size: 210 4163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2581 2891 3037 4183 2581
Size: 310 146 1146 2771
Fragments arranged by size:
2771 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1046 1385 1046
Size: 339 4034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 744 1092 1358 1372 2198 2630 3377 3645
Size: 79 348 266 14 826 432 747 268
Cuts at: 3645 665
Size: 1393
Fragments arranged by size:
1393 826 747 432 348 268 266 79
14
EcoO109I rG'GnC_Cy
Cuts at: 782 1512 782
Size: 730 3643
EcoRI G'AATT_C
Cuts at: 1377 1377
Size: 4373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 580 2392 580
Size: 1812 2561
FspI TGC'GCA
Cuts at: 2154 2154
Size: 4373
HaeII r_GCGC'y
Cuts at: 1807 2055 3077 4056 1807
Size: 248 1022 979 2124
Fragments arranged by size:
2124 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2215 2245 2247 2277 2215
Size: 30 2 30 4311
Fragments arranged by size:
4311 30 30 2
HincII GTy'rAC
Cuts at: 753 1412 753
Size: 659 3714
HpaI GTT'AAC
Cuts at: 753 753
Size: 4373
KasI G'GCGC_C
Cuts at: 1803 2051 1803
Size: 248 4125
KpnI G_GTAC'C
Cuts at: 1393 1393
Size: 4373
MfeI C'AATT_G
Cuts at: 491 491
Size: 4373
MluI A'CGCG_T
Cuts at: 3806 3806
Size: 4373
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3044 3228 3044
Size: 184 4189
MscI TGG'CCA
Cuts at: 436 2134 436
Size: 1698 2675
MslI CAynn'nnrTG
Cuts at: 2362 2489 2362
Size: 127 4246
MspA1I CmG'CkG
Cuts at: 443 795 1484 1798 2158 3171 3416 443
Size: 352 689 314 360 1013 245 1400
Fragments arranged by size:
1400 1013 689 360 352 314 245
NaeI GCC'GGC
Cuts at: 2554 2554
Size: 4373
NarI GG'CG_CC
Cuts at: 1804 2052 1804
Size: 248 4125
NgoMIV G'CCGG_C
Cuts at: 2552 2552
Size: 4373
NsiI A_TGCA'T
Cuts at: 987 987
Size: 4373
NspI r_CATG'y
Cuts at: 127 695 1426 1926 2833 127
Size: 568 731 500 907 1667
Fragments arranged by size:
1667 907 731 568 500
PciI A'CATG_T
Cuts at: 2829 2829
Size: 4373
PflMI CCAn_nnn'nTGG
Cuts at: 443 443
Size: 4373
PfoI T'CCnGG_A
Cuts at: 480 480
Size: 4373
PmeI GTTT'AAAC
Cuts at: 1372 1372
Size: 4373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 409 441 3512 3544 409
Size: 32 3071 32 1238
Fragments arranged by size:
3071 1238 32 32
PstI C_TGCA'G
Cuts at: 416 1420 2105 416
Size: 1004 685 2684
Fragments arranged by size:
2684 1004 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4373
PvuII CAG'CTG
Cuts at: 443 795 1798 2158 443
Size: 352 1003 360 2658
Fragments arranged by size:
2658 1003 360 352
RsrII CG'GwC_CG
Cuts at: 2568 2568
Size: 4373
SacI G_AGCT'C
Cuts at: 1048 1387 1048
Size: 339 4034
SalI G'TCGA_C
Cuts at: 1410 1410
Size: 4373
SapI GCTCTTCn'nnn_
Cuts at: 2396 2606 2396
Size: 210 4163
SbfI CC_TGCA'GG
Cuts at: 1420 1420
Size: 4373
ScaI AGT'ACT
Cuts at: 3890 3890
Size: 4373
SfcI C'TryA_G
Cuts at: 33 412 521 624 1416 2101 3094 3285 33
Size: 379 109 103 792 685 993 191 1121
Fragments arranged by size:
1121 993 792 685 379 191 109 103
SfoI GGC'GCC
Cuts at: 1805 2053 1805
Size: 248 4125
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4373
SmaI CCC'GGG
Cuts at: 1395 1395
Size: 4373
SmlI C'TyrA_G
Cuts at: 239 1122 1528 2443 2935 3197 239
Size: 883 406 915 492 262 1415
Fragments arranged by size:
1415 915 883 492 406 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4373
SphI G_CATG'C
Cuts at: 695 1426 1926 695
Size: 731 500 3142
Fragments arranged by size:
3142 731 500
StyI C'CwwG_G
Cuts at: 1507 1861 1507
Size: 354 4019
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1956 2089 2548 2757 1956
Size: 133 459 209 3572
Fragments arranged by size:
3572 459 209 133
TatI w'GTAC_w
Cuts at: 3888 3888
Size: 4373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3890 3922 3890
Size: 32 4341
Tth111I GACn'n_nGTC
Cuts at: 2170 2170
Size: 4373
XbaI T'CTAG_A
Cuts at: 294 1404 294
Size: 1110 3263
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1868 114
Size: 1754 2619
XmaI C'CCGG_G
Cuts at: 1393 1393
Size: 4373
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BsaXI
BseRI BsiWI BsrGI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
NcoI NdeI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI