Restriction map for
pF1KB6650
(Circular) (Six-base) MAPSORT of: pF1KB6650.seq Check: 9074 from: 1 to: 4451
pF1KB6650 4451 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 221 221
Size: 4451
AccI GT'mk_AC
Cuts at: 1489 1489
Size: 4451
Acc65I G'GTAC_C
Cuts at: 1467 1467
Size: 4451
AclI AA'CG_TT
Cuts at: 4291 4291
Size: 4451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 790 1178 2276 2708 3455 3723 604
Size: 186 388 1098 432 747 268 1332
Fragments arranged by size:
1332 1098 747 432 388 268 186
AflIII A'CryG_T
Cuts at: 223 1427 1803 2907 3884 223
Size: 1204 376 1104 977 790
Fragments arranged by size:
1204 1104 977 790 376
AgeI A'CCGG_T
Cuts at: 2839 2839
Size: 4451
AlwNI CAG_nnn'CTG
Cuts at: 582 1756 3323 582
Size: 1174 1567 1710
Fragments arranged by size:
1710 1567 1174
ApaLI G'TGCA_C
Cuts at: 3221 3221
Size: 4451
ApoI r'AATT_y
Cuts at: 1455 3804 4060 1455
Size: 2349 256 1846
Fragments arranged by size:
2349 1846 256
AseI AT'TA_AT
Cuts at: 20 1015 20
Size: 995 3456
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
AvaI C'yCGr_G
Cuts at: 952 1471 952
Size: 519 3932
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 579 612 579
Size: 33 4418
BamHI G'GATC_C
Cuts at: 1476 1476
Size: 4451
BanI G'GyrC_C
Cuts at: 1467 1881 2129 2164 1467
Size: 414 248 35 3754
Fragments arranged by size:
3754 414 248 35
BanII G_rGCy'C
Cuts at: 638 1465 2495 638
Size: 827 1030 2594
Fragments arranged by size:
2594 1030 827
BbeI G_GCGC'C
Cuts at: 1885 2133 1885
Size: 248 4203
BbsI GAAGACnn'nnnn_
Cuts at: 304 506 1427 304
Size: 202 921 3328
Fragments arranged by size:
3328 921 202
BciVI GTATCCnnnnn_n'
Cuts at: 1264 2349 3110 4451 1264
Size: 1085 761 1341 1264
Fragments arranged by size:
1341 1264 1085 761
BclI T'GATC_A
Cuts at: 1326 1971 1326
Size: 645 3806
BglII A'GATC_T
Cuts at: 1388 1412 1966 4003 1388
Size: 24 554 2037 1836
Fragments arranged by size:
2037 1836 554 24
BlpI GC'TnA_GC
Cuts at: 632 1563 632
Size: 931 3520
Bme1580I G_kGCm'C
Cuts at: 2076 2169 3225 2076
Size: 93 1056 3302
Fragments arranged by size:
3302 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1663 1744 2079 4223 1663
Size: 81 335 2144 1891
Fragments arranged by size:
2144 1891 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 4451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 589 230
Size: 359 4092
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 809 1627 2506 2998 3296 809
Size: 818 879 492 298 1964
Fragments arranged by size:
1964 879 818 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 109 4155 109
Size: 4046 405
BsaAI yAC'GTr
Cuts at: 1428 1804 1428
Size: 376 4075
BsaBI GATnn'nnATC
Cuts at: 6 277 787 1965 4002 6
Size: 271 510 1178 2037 455
Fragments arranged by size:
2037 1178 510 455 271
BsaHI Gr'CG_yC
Cuts at: 218 1882 2130 2832 4328 218
Size: 1664 248 702 1496 341
Fragments arranged by size:
1664 1496 702 341 248
BsaWI w'CCGG_w
Cuts at: 1640 2161 2839 3113 3260 3671 1640
Size: 521 678 274 147 411 2420
Fragments arranged by size:
2420 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 203 233 203
Size: 30 4421
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 667 127
Size: 540 3911
BseYI C'CCAG_C
Cuts at: 242 812 1273 1395 1876 2634 3211 3707 242
Size: 570 461 122 481 758 577 496 986
Fragments arranged by size:
986 758 577 570 496 481 461 122
BsiEI CG_ry'CG
Cuts at: 104 1993 2039 3247 104
Size: 1889 46 1208 1308
Fragments arranged by size:
1889 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 638 1465 2243 2433 3225 638
Size: 827 778 190 792 1864
Fragments arranged by size:
1864 827 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 638 1465 2076 2169 2243 2433 2495 3225 638
Size: 827 611 93 74 190 62 730 1864
Fragments arranged by size:
1864 827 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3671 3671
Size: 4451
BspHI T'CATG_A
Cuts at: 3627 3627
Size: 4451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 407 1501 1715 2017 2398 407
Size: 1094 214 302 381 2460
Fragments arranged by size:
2460 1094 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1281 2743 2797 4281 4446 50
Size: 1231 1462 54 1484 165 55
Fragments arranged by size:
1484 1462 1231 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1001 2363 1001
Size: 1362 3089
BsrFI r'CCGG_y
Cuts at: 2449 2630 2839 2449
Size: 181 209 4061
Fragments arranged by size:
4061 209 181
BssSI C'ACGA_G
Cuts at: 2722 3080 2722
Size: 358 4093
BstAPI GCAn_nnn'nTGC
Cuts at: 1828 1828
Size: 4451
BstBI TT'CG_AA
Cuts at: 2812 2812
Size: 4451
BstYI r'GATC_y
Cuts at: 1 126 1388 1412 1476 1943 1966 2301 2547
Size: 125 1262 24 64 467 23 335 246
Cuts at: 2547 3559 3645 3657 4003 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1262 1012 467 449 346 335 246 125
86 64 24 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2401 2582 2745 2401
Size: 181 163 4107
Fragments arranged by size:
4107 181 163
BtsI GCAGTG_nn'
Cuts at: 1694 1694
Size: 4451
DraI TTT'AAA
Cuts at: 1450 1450
Size: 4451
DrdI GACnn_nn'nnGTC
Cuts at: 2157 3015 2157
Size: 858 3593
EaeI y'GGCC_r
Cuts at: 2036 2210 2601 2628 3973 2036
Size: 174 391 27 1345 2514
Fragments arranged by size:
2514 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2036 2036
Size: 4451
EarI CTCTTCn'nnn_
Cuts at: 530 1117 2474 2684 530
Size: 587 1357 210 2297
Fragments arranged by size:
2297 1357 587 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2659 2969 3115 4261 2659
Size: 310 146 1146 2849
Fragments arranged by size:
2849 1146 310 146
EcoICRI GAG'CTC
Cuts at: 636 1463 636
Size: 827 3624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 589 604 790 1178 2276 2708 3455 3723
Size: 359 15 186 388 1098 432 747 268
Cuts at: 3723 230
Size: 958
Fragments arranged by size:
1098 958 747 432 388 359 268 186
15
EcoNI CCTnn'n_nnAGG
Cuts at: 403 403
Size: 4451
EcoO109I rG'GnC_Cy
Cuts at: 1590 1590
Size: 4451
EcoRI G'AATT_C
Cuts at: 1455 1455
Size: 4451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 488 1431 2470 488
Size: 943 1039 2469
Fragments arranged by size:
2469 1039 943
FspI TGC'GCA
Cuts at: 2232 2232
Size: 4451
HaeII r_GCGC'y
Cuts at: 1885 2133 3155 4134 1885
Size: 248 1022 979 2202
Fragments arranged by size:
2202 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 203 235 2293 2323 2325 2355 203
Size: 32 2058 30 2 30 2299
Fragments arranged by size:
2299 2058 32 30 30 2
HincII GTy'rAC
Cuts at: 821 1490 821
Size: 669 3782
HindIII A'AGCT_T
Cuts at: 389 844 389
Size: 455 3996
KasI G'GCGC_C
Cuts at: 1881 2129 1881
Size: 248 4203
KpnI G_GTAC'C
Cuts at: 1471 1471
Size: 4451
MfeI C'AATT_G
Cuts at: 1368 1368
Size: 4451
MluI A'CGCG_T
Cuts at: 3884 3884
Size: 4451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 668 3122 3306 668
Size: 2454 184 1813
Fragments arranged by size:
2454 1813 184
MscI TGG'CCA
Cuts at: 2212 2212
Size: 4451
MslI CAynn'nnrTG
Cuts at: 322 2440 2567 322
Size: 2118 127 2206
Fragments arranged by size:
2206 2118 127
MspA1I CmG'CkG
Cuts at: 142 593 816 888 1562 1876 2236 3249 3494
Size: 451 223 72 674 314 360 1013 245
Cuts at: 3494 142
Size: 1099
Fragments arranged by size:
1099 1013 674 451 360 314 245 223
72
NaeI GCC'GGC
Cuts at: 2632 2632
Size: 4451
NarI GG'CG_CC
Cuts at: 1882 2130 1882
Size: 248 4203
NcoI C'CATG_G
Cuts at: 107 107
Size: 4451
NgoMIV G'CCGG_C
Cuts at: 2630 2630
Size: 4451
NspI r_CATG'y
Cuts at: 227 627 1504 2004 2911 227
Size: 400 877 500 907 1767
Fragments arranged by size:
1767 907 877 500 400
PciI A'CATG_T
Cuts at: 223 2907 223
Size: 2684 1767
PflMI CCAn_nnn'nTGG
Cuts at: 1249 1249
Size: 4451
PfoI T'CCnGG_A
Cuts at: 1221 1221
Size: 4451
PmeI GTTT'AAAC
Cuts at: 1450 1450
Size: 4451
PmlI CAC'GTG
Cuts at: 1428 1428
Size: 4451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4419
PspXI vC'TCGA_Gb
Cuts at: 952 952
Size: 4451
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 409 441 409
Size: 32 4419
PstI C_TGCA'G
Cuts at: 1082 1498 2183 1082
Size: 416 685 3350
Fragments arranged by size:
3350 685 416
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4451
PvuII CAG'CTG
Cuts at: 142 593 816 888 1876 2236 142
Size: 451 223 72 988 360 2357
Fragments arranged by size:
2357 988 451 360 223 72
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 4451
SacI G_AGCT'C
Cuts at: 638 1465 638
Size: 827 3624
SalI G'TCGA_C
Cuts at: 1488 1488
Size: 4451
SapI GCTCTTCn'nnn_
Cuts at: 2474 2684 2474
Size: 210 4241
SbfI CC_TGCA'GG
Cuts at: 1498 1498
Size: 4451
ScaI AGT'ACT
Cuts at: 3968 3968
Size: 4451
SfcI C'TryA_G
Cuts at: 33 667 1078 1400 1494 2179 3172 3363 33
Size: 634 411 322 94 685 993 191 1121
Fragments arranged by size:
1121 993 685 634 411 322 191 94
SfoI GGC'GCC
Cuts at: 1883 2131 1883
Size: 248 4203
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
SmaI CCC'GGG
Cuts at: 1473 1473
Size: 4451
SmlI C'TyrA_G
Cuts at: 824 952 1606 2521 3013 3275 824
Size: 128 654 915 492 262 2000
Fragments arranged by size:
2000 915 654 492 262 128
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4451
SphI G_CATG'C
Cuts at: 1504 2004 1504
Size: 500 3951
StyI C'CwwG_G
Cuts at: 107 404 1334 1585 1939 107
Size: 297 930 251 354 2619
Fragments arranged by size:
2619 930 354 297 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 256 2034 2167 2626 2835 256
Size: 1778 133 459 209 1872
Fragments arranged by size:
1872 1778 459 209 133
TatI w'GTAC_w
Cuts at: 3966 3966
Size: 4451
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 779 811 3968 4000 779
Size: 32 3157 32 1230
Fragments arranged by size:
3157 1230 32 32
Tth111I GACn'n_nGTC
Cuts at: 2248 2248
Size: 4451
XbaI T'CTAG_A
Cuts at: 1482 1482
Size: 4451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1946 1946
Size: 4451
XhoI C'TCGA_G
Cuts at: 952 952
Size: 4451
XmaI C'CCGG_G
Cuts at: 1471 1471
Size: 4451
ZraI GAC'GTC
Cuts at: 219 219
Size: 4451
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PasI PpuMI PshAI PsiI PspOMI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI