Restriction map for
pF1KB6645
(Circular) (Six-base) MAPSORT of: pF1KB6645.seq Check: 3908 from: 1 to: 4370
pF1KB6645 4370 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 349 349
Size: 4370
AccI GT'mk_AC
Cuts at: 1408 1408
Size: 4370
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1113 2195 2627 3374 3642 268
Size: 845 1082 432 747 268 996
Fragments arranged by size:
1082 996 845 747 432 268
AflIII A'CryG_T
Cuts at: 233 1722 2826 3803 233
Size: 1489 1104 977 800
Fragments arranged by size:
1489 1104 977 800
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 955 1276 955
Size: 321 4049
AlwNI CAG_nnn'CTG
Cuts at: 1675 3242 1675
Size: 1567 2803
ApaLI G'TGCA_C
Cuts at: 3140 3140
Size: 4370
ApoI r'AATT_y
Cuts at: 1374 3723 3979 1374
Size: 2349 256 1765
Fragments arranged by size:
2349 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 1390 1390
Size: 4370
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 625 741 834 1386 1800 2048 2083 625
Size: 116 93 552 414 248 35 2912
Fragments arranged by size:
2912 552 414 248 116 93 35
BanII G_rGCy'C
Cuts at: 329 1384 2414 329
Size: 1055 1030 2285
Fragments arranged by size:
2285 1055 1030
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BbsI GAAGACnn'nnnn_
Cuts at: 642 706 1087 1105 642
Size: 64 381 18 3907
Fragments arranged by size:
3907 381 64 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1212 1246 1212
Size: 34 4336
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3029 4370 2268
Size: 761 1341 2268
Fragments arranged by size:
2268 1341 761
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BglII A'GATC_T
Cuts at: 1226 1885 3922 1226
Size: 659 2037 1674
Fragments arranged by size:
2037 1674 659
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4370
Bme1580I G_kGCm'C
Cuts at: 300 953 1255 1995 2088 3144 300
Size: 653 302 740 93 1056 1526
Fragments arranged by size:
1526 1056 740 653 302 93
BmgBI CAC'GTC
Cuts at: 1011 1011
Size: 4370
BmrI ACTGGGnnnn_n'
Cuts at: 1302 1582 1663 1998 4142 1302
Size: 280 81 335 2144 1530
Fragments arranged by size:
2144 1530 335 280 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1003
Size: 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1054 1306 1546 2425 2917 3215 982
Size: 72 252 240 879 492 298 2137
Fragments arranged by size:
2137 879 492 298 252 240 72
BsaI GGTCTCn'nnnn_
Cuts at: 676 875 4074 676
Size: 199 3199 972
Fragments arranged by size:
3199 972 199
BsaAI yAC'GTr
Cuts at: 1035 1723 1035
Size: 688 3682
BsaBI GATnn'nnATC
Cuts at: 6 781 1884 3921 6
Size: 775 1103 2037 455
Fragments arranged by size:
2037 1103 775 455
BsaHI Gr'CG_yC
Cuts at: 1801 2049 2751 4247 1801
Size: 248 702 1496 1924
Fragments arranged by size:
1924 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3032 3179 3590 1559
Size: 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 220 250 787 817 220
Size: 30 537 30 3773
Fragments arranged by size:
3773 537 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 520 886 919 1345 1348 517
Size: 3 366 33 426 3 3539
Fragments arranged by size:
3539 426 366 33 3 3
BseYI C'CCAG_C
Cuts at: 214 322 918 1200 1795 2553 3130 3626 214
Size: 108 596 282 595 758 577 496 958
Fragments arranged by size:
958 758 596 595 577 496 282 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 915 915
Size: 4370
BsiEI CG_ry'CG
Cuts at: 104 959 1912 1958 3166 104
Size: 855 953 46 1208 1308
Fragments arranged by size:
1308 1208 953 855 46
BsiHKAI G_wGCw'C
Cuts at: 1384 2162 2352 3144 1384
Size: 778 190 792 2610
Fragments arranged by size:
2610 792 778 190
BsiWI C'GTAC_G
Cuts at: 1031 1031
Size: 4370
BsmI GAATG_Cn'
Cuts at: 423 423
Size: 4370
Bsp1286I G_dGCh'C
Cuts at: 300 329 953 1255 1384 1995 2088 2162 2352
Size: 29 624 302 129 611 93 74 190
Cuts at: 2352 2414 3144 300
Size: 62 730 1526
Fragments arranged by size:
1526 730 624 611 302 190 129 93
74 62 29
BspEI T'CCGG_A
Cuts at: 3590 3590
Size: 4370
BspHI T'CATG_A
Cuts at: 3546 3546
Size: 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 349 893 1420 1634 1936 2317 349
Size: 544 527 214 302 381 2402
Fragments arranged by size:
2402 544 527 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2662 2716 4200 4365 50
Size: 2612 54 1484 165 55
Fragments arranged by size:
2612 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 308 1248 2282 308
Size: 940 1034 2396
Fragments arranged by size:
2396 1034 940
BsrFI r'CCGG_y
Cuts at: 2368 2549 2758 2368
Size: 181 209 3980
Fragments arranged by size:
3980 209 181
BssSI C'ACGA_G
Cuts at: 2641 2999 2641
Size: 358 4012
BstAPI GCAn_nnn'nTGC
Cuts at: 365 1747 365
Size: 1382 2988
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstEII G'GTnAC_C
Cuts at: 1176 1176
Size: 4370
BstXI CCAn_nnnn'nTGG
Cuts at: 315 757 315
Size: 442 3928
BstYI r'GATC_y
Cuts at: 1 1040 1139 1226 1395 1862 1885 2220 2466
Size: 1039 99 87 169 467 23 335 246
Cuts at: 2466 3478 3564 3576 3922 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1039 1012 467 449 346 335 246 169
99 87 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1328 1328
Size: 4370
BtgI C'CryG_G
Cuts at: 185 953 1247 185
Size: 768 294 3308
Fragments arranged by size:
3308 768 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1287 2320 2501 2664 1287
Size: 1033 181 163 2993
Fragments arranged by size:
2993 1033 181 163
BtsI GCAGTG_nn'
Cuts at: 1613 1613
Size: 4370
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 640 2076 2934 640
Size: 1436 858 2076
Fragments arranged by size:
2076 1436 858
EaeI y'GGCC_r
Cuts at: 779 858 956 1283 1955 2129 2520 2547 3892
Size: 79 98 327 672 174 391 27 1345
Cuts at: 3892 779
Size: 1257
Fragments arranged by size:
1345 1257 672 391 327 174 98 79
27
EagI C'GGCC_G
Cuts at: 956 1955 956
Size: 999 3371
EarI CTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 2888 3034 4180 2578
Size: 310 146 1146 2768
Fragments arranged by size:
2768 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1382 1382
Size: 4370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 1003 1113 2195 2627 3374 3642 268
Size: 735 110 1082 432 747 268 996
Fragments arranged by size:
1082 996 747 735 432 268 110
EcoNI CCTnn'n_nnAGG
Cuts at: 481 481
Size: 4370
EcoO109I rG'GnC_Cy
Cuts at: 1509 1509
Size: 4370
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 300 2389 300
Size: 2089 2281
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 1804 2052 3074 4053 1804
Size: 248 1022 979 2121
Fragments arranged by size:
2121 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 787 819 2212 2242 2244 2274 787
Size: 32 1393 30 2 30 2883
Fragments arranged by size:
2883 1393 32 30 30 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4370
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1298 3041 3225 1298
Size: 1743 184 2443
Fragments arranged by size:
2443 1743 184
MscI TGG'CCA
Cuts at: 781 1285 2131 781
Size: 504 846 3020
Fragments arranged by size:
3020 846 504
MslI CAynn'nnrTG
Cuts at: 313 427 2359 2486 313
Size: 114 1932 127 2197
Fragments arranged by size:
2197 1932 127 114
MspA1I CmG'CkG
Cuts at: 1481 1795 2155 3168 3413 1481
Size: 314 360 1013 245 2438
Fragments arranged by size:
2438 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4370
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4370
NspI r_CATG'y
Cuts at: 237 1281 1423 1923 2830 237
Size: 1044 142 500 907 1777
Fragments arranged by size:
1777 1044 907 500 142
PasI CC'CwG_GG
Cuts at: 293 293
Size: 4370
PciI A'CATG_T
Cuts at: 233 2826 233
Size: 2593 1777
PflMI CCAn_nnn'nTGG
Cuts at: 1173 1173
Size: 4370
PfoI T'CCnGG_A
Cuts at: 980 980
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 4338
PshAI GACnn'nnGTC
Cuts at: 688 688
Size: 4370
PstI C_TGCA'G
Cuts at: 539 593 1417 2102 539
Size: 54 824 685 2807
Fragments arranged by size:
2807 824 685 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 1795 2155 1795
Size: 360 4010
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1384 1384
Size: 4370
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 535 589 1413 2098 3091 3282 33
Size: 502 54 824 685 993 191 1121
Fragments arranged by size:
1121 993 824 685 502 191 54
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 997 1069 1321 1525 2440 2932 3194 997
Size: 72 252 204 915 492 262 2173
Fragments arranged by size:
2173 915 492 262 252 204 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StuI AGG'CCT
Cuts at: 476 476
Size: 4370
StyI C'CwwG_G
Cuts at: 1504 1858 1504
Size: 354 4016
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1953 2086 2545 2754 1953
Size: 133 459 209 3569
Fragments arranged by size:
3569 459 209 133
TatI w'GTAC_w
Cuts at: 3885 3885
Size: 4370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4338
Tth111I GACn'n_nGTC
Cuts at: 2167 2167
Size: 4370
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 183 1865 183
Size: 1682 2688
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
XmnI GAAnn'nnTTC
Cuts at: 118 259 118
Size: 141 4229
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsmBI
BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI