Restriction map for
pF1KB6642
(Circular) (Six-base) MAPSORT of: pF1KB6642.seq Check: 2594 from: 1 to: 4442
pF1KB6642 4442 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 565 565
Size: 4442
AccI GT'mk_AC
Cuts at: 1211 1480 1211
Size: 269 4173
Acc65I G'GTAC_C
Cuts at: 1458 1458
Size: 4442
AclI AA'CG_TT
Cuts at: 4282 4282
Size: 4442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 2267 2699 3446 3714 218
Size: 2049 432 747 268 946
Fragments arranged by size:
2049 946 747 432 268
AfeI AGC'GCT
Cuts at: 754 754
Size: 4442
AflIII A'CryG_T
Cuts at: 1184 1794 2898 3875 1184
Size: 610 1104 977 1751
Fragments arranged by size:
1751 1104 977 610
AgeI A'CCGG_T
Cuts at: 2830 2830
Size: 4442
AlwNI CAG_nnn'CTG
Cuts at: 498 933 1747 3314 498
Size: 435 814 1567 1626
Fragments arranged by size:
1626 1567 814 435
ApaI G_GGCC'C
Cuts at: 587 741 1330 587
Size: 154 589 3699
Fragments arranged by size:
3699 589 154
ApaLI G'TGCA_C
Cuts at: 552 1138 3212 552
Size: 586 2074 1782
Fragments arranged by size:
2074 1782 586
ApoI r'AATT_y
Cuts at: 1368 1446 3795 4051 1368
Size: 78 2349 256 1759
Fragments arranged by size:
2349 1759 256 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 4442
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
AvaI C'yCGr_G
Cuts at: 133 586 1331 1462 133
Size: 453 745 131 3113
Fragments arranged by size:
3113 745 453 131
BamHI G'GATC_C
Cuts at: 1467 1467
Size: 4442
BanI G'GyrC_C
Cuts at: 1219 1458 1872 2120 2155 1219
Size: 239 414 248 35 3506
Fragments arranged by size:
3506 414 248 239 35
BanII G_rGCy'C
Cuts at: 206 452 587 741 1061 1330 1456 2486 206
Size: 246 135 154 320 269 126 1030 2162
Fragments arranged by size:
2162 1030 320 269 246 154 135 126
BbeI G_GCGC'C
Cuts at: 1876 2124 1876
Size: 248 4194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 490 524 90
Size: 34 366 34 4008
Fragments arranged by size:
4008 366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2340 3101 4442 2340
Size: 761 1341 2340
Fragments arranged by size:
2340 1341 761
BclI T'GATC_A
Cuts at: 1962 1962
Size: 4442
BglI GCCn_nnn'nGGC
Cuts at: 440 797 440
Size: 357 4085
BglII A'GATC_T
Cuts at: 1957 3994 1957
Size: 2037 2405
BlpI GC'TnA_GC
Cuts at: 1554 1554
Size: 4442
Bme1580I G_kGCm'C
Cuts at: 492 556 587 741 1142 1178 1330 2067 2160
Size: 64 31 154 401 36 152 737 93
Cuts at: 2160 3216 492
Size: 1056 1718
Fragments arranged by size:
1718 1056 737 401 154 152 93 64
36 31
BmgBI CAC'GTC
Cuts at: 1185 1185
Size: 4442
BmrI ACTGGGnnnn_n'
Cuts at: 1654 1735 2070 4214 1654
Size: 81 335 2144 1882
Fragments arranged by size:
2144 1882 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 4442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 840 1372 840
Size: 532 3910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1618 2497 2989 3287 1618
Size: 879 492 298 2773
Fragments arranged by size:
2773 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 143 1077 4146 143
Size: 934 3069 439
Fragments arranged by size:
3069 934 439
BsaAI yAC'GTr
Cuts at: 1341 1795 1341
Size: 454 3988
BsaBI GATnn'nnATC
Cuts at: 6 1956 3993 6
Size: 1950 2037 455
Fragments arranged by size:
2037 1950 455
BsaHI Gr'CG_yC
Cuts at: 1873 2121 2823 4319 1873
Size: 248 702 1496 1996
Fragments arranged by size:
1996 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1631 2152 2830 3104 3251 3662 1631
Size: 521 678 274 147 411 2411
Fragments arranged by size:
2411 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 688 718 973 1003 688
Size: 30 255 30 4127
Fragments arranged by size:
4127 255 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 176 176
Size: 4442
BseYI C'CCAG_C
Cuts at: 514 687 1867 2625 3202 3698 514
Size: 173 1180 758 577 496 1258
Fragments arranged by size:
1258 1180 758 577 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 484
Size: 4442
BsiEI CG_ry'CG
Cuts at: 104 1318 1984 2030 3238 104
Size: 1214 666 46 1208 1308
Fragments arranged by size:
1308 1214 1208 666 46
BsiHKAI G_wGCw'C
Cuts at: 556 991 1142 1456 2234 2424 3216 556
Size: 435 151 314 778 190 792 1782
Fragments arranged by size:
1782 792 778 435 314 190 151
BsmI GAATG_Cn'
Cuts at: 226 226
Size: 4442
Bsp1286I G_dGCh'C
Cuts at: 206 452 492 556 587 741 991 1061 1142
Size: 246 40 64 31 154 250 70 81
Cuts at: 1142 1178 1330 1456 2067 2160 2234 2424 2486
Size: 36 152 126 611 93 74 190 62
Cuts at: 2486 3216 206
Size: 730 1432
Fragments arranged by size:
1432 730 611 250 246 190 154 152
126 93 81 74 70 64 62 40
36 31
BspEI T'CCGG_A
Cuts at: 3662 3662
Size: 4442
BspHI T'CATG_A
Cuts at: 1376 3618 1376
Size: 2242 2200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 565 653 1492 1706 2008 2389 565
Size: 88 839 214 302 381 2618
Fragments arranged by size:
2618 839 381 302 214 88
BsrBI CCG'CTC
Cuts at: 50 356 415 704 1167 2734 2788 4272 4437
Size: 306 59 289 463 1567 54 1484 165
Cuts at: 4437 50
Size: 55
Fragments arranged by size:
1567 1484 463 306 289 165 59 55
54
BsrDI GCAATG_nn'
Cuts at: 2354 2354
Size: 4442
BsrFI r'CCGG_y
Cuts at: 1317 2440 2621 2830 1317
Size: 1123 181 209 2929
Fragments arranged by size:
2929 1123 209 181
BssSI C'ACGA_G
Cuts at: 2713 3071 2713
Size: 358 4084
BstAPI GCAn_nnn'nTGC
Cuts at: 1819 1819
Size: 4442
BstBI TT'CG_AA
Cuts at: 2803 2803
Size: 4442
BstEII G'GTnAC_C
Cuts at: 568 922 1359 568
Size: 354 437 3651
Fragments arranged by size:
3651 437 354
BstYI r'GATC_y
Cuts at: 1 1193 1238 1467 1934 1957 2292 2538 3550
Size: 1192 45 229 467 23 335 246 1012
Cuts at: 3550 3636 3648 3994 1
Size: 86 12 346 449
Fragments arranged by size:
1192 1012 467 449 346 335 246 229
86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 188 293 188
Size: 105 4337
BtgI C'CryG_G
Cuts at: 1017 1017
Size: 4442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2392 2573 2736 2392
Size: 181 163 4098
Fragments arranged by size:
4098 181 163
BtsI GCAGTG_nn'
Cuts at: 258 837 909 1685 258
Size: 579 72 776 3015
Fragments arranged by size:
3015 776 579 72
DraI TTT'AAA
Cuts at: 1441 1441
Size: 4442
DrdI GACnn_nn'nnGTC
Cuts at: 2148 3006 2148
Size: 858 3584
EaeI y'GGCC_r
Cuts at: 935 1315 2027 2201 2592 2619 3964 935
Size: 380 712 174 391 27 1345 1413
Fragments arranged by size:
1413 1345 712 391 380 174 27
EagI C'GGCC_G
Cuts at: 1315 2027 1315
Size: 712 3730
EarI CTCTTCn'nnn_
Cuts at: 194 2465 2675 194
Size: 2271 210 1961
Fragments arranged by size:
2271 1961 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2650 2960 3106 4252 2650
Size: 310 146 1146 2840
Fragments arranged by size:
2840 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1454 1454
Size: 4442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 840 1372 2267 2699 3446 3714 218
Size: 622 532 895 432 747 268 946
Fragments arranged by size:
946 895 747 622 532 432 268
EcoO109I rG'GnC_Cy
Cuts at: 243 391 495 691 737 738 773 1327 1424
Size: 148 104 196 46 1 35 554 97
Cuts at: 1424 1581 243
Size: 157 3104
Fragments arranged by size:
3104 554 196 157 148 104 97 46
35 1
EcoRI G'AATT_C
Cuts at: 1368 1446 1368
Size: 78 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4442
FspI TGC'GCA
Cuts at: 980 2223 980
Size: 1243 3199
FspAI rTGC'GCAy
Cuts at: 980 980
Size: 4442
HaeII r_GCGC'y
Cuts at: 157 756 1298 1876 2124 3146 4125 157
Size: 599 542 578 248 1022 979 474
Fragments arranged by size:
1022 979 599 578 542 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 808 840 955 987 2284 2314 2316 2346 808
Size: 32 115 32 1297 30 2 30 2904
Fragments arranged by size:
2904 1297 115 32 32 30 30 2
HincII GTy'rAC
Cuts at: 273 885 1011 1481 273
Size: 612 126 470 3234
Fragments arranged by size:
3234 612 470 126
KasI G'GCGC_C
Cuts at: 1872 2120 1872
Size: 248 4194
KpnI G_GTAC'C
Cuts at: 1462 1462
Size: 4442
MluI A'CGCG_T
Cuts at: 3875 3875
Size: 4442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1127 3113 3297 1127
Size: 1986 184 2272
Fragments arranged by size:
2272 1986 184
MscI TGG'CCA
Cuts at: 937 2203 937
Size: 1266 3176
MslI CAynn'nnrTG
Cuts at: 815 2431 2558 815
Size: 1616 127 2699
Fragments arranged by size:
2699 1616 127
MspA1I CmG'CkG
Cuts at: 361 467 635 662 687 878 930 1031 1049
Size: 106 168 27 25 191 52 101 18
Cuts at: 1049 1085 1180 1553 1867 2227 3240 3485 361
Size: 36 95 373 314 360 1013 245 1318
Fragments arranged by size:
1318 1013 373 360 314 245 191 168
106 101 95 52 36 27 25 18
NaeI GCC'GGC
Cuts at: 2623 2623
Size: 4442
NarI GG'CG_CC
Cuts at: 1873 2121 1873
Size: 248 4194
NcoI C'CATG_G
Cuts at: 1017 1017
Size: 4442
NgoMIV G'CCGG_C
Cuts at: 2621 2621
Size: 4442
NspI r_CATG'y
Cuts at: 1495 1995 2902 1495
Size: 500 907 3035
Fragments arranged by size:
3035 907 500
PciI A'CATG_T
Cuts at: 2898 2898
Size: 4442
PflMI CCAn_nnn'nTGG
Cuts at: 933 933
Size: 4442
PmeI GTTT'AAAC
Cuts at: 1441 1441
Size: 4442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4410
PpuMI rG'GwC_Cy
Cuts at: 243 391 773 243
Size: 148 382 3912
Fragments arranged by size:
3912 382 148
PshAI GACnn'nnGTC
Cuts at: 1207 1207
Size: 4442
PspOMI G'GGCC_C
Cuts at: 583 737 1326 583
Size: 154 589 3699
Fragments arranged by size:
3699 589 154
PstI C_TGCA'G
Cuts at: 832 1489 2174 832
Size: 657 685 3100
Fragments arranged by size:
3100 685 657
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4442
PvuII CAG'CTG
Cuts at: 467 687 930 1867 2227 467
Size: 220 243 937 360 2682
Fragments arranged by size:
2682 937 360 243 220
RsrII CG'GwC_CG
Cuts at: 2637 2637
Size: 4442
SacI G_AGCT'C
Cuts at: 1456 1456
Size: 4442
SalI G'TCGA_C
Cuts at: 1479 1479
Size: 4442
SanDI GG'GwC_CC
Cuts at: 243 391 243
Size: 148 4294
SapI GCTCTTCn'nnn_
Cuts at: 194 2465 2675 194
Size: 2271 210 1961
Fragments arranged by size:
2271 1961 210
SbfI CC_TGCA'GG
Cuts at: 1489 1489
Size: 4442
ScaI AGT'ACT
Cuts at: 3959 3959
Size: 4442
SfcI C'TryA_G
Cuts at: 33 828 1485 2170 3163 3354 33
Size: 795 657 685 993 191 1121
Fragments arranged by size:
1121 993 795 685 657 191
SfoI GGC'GCC
Cuts at: 1874 2122 1874
Size: 248 4194
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
SmaI CCC'GGG
Cuts at: 135 1333 1464 135
Size: 1198 131 3113
Fragments arranged by size:
3113 1198 131
SmlI C'TyrA_G
Cuts at: 1597 2512 3004 3266 1597
Size: 915 492 262 2773
Fragments arranged by size:
2773 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4442
SphI G_CATG'C
Cuts at: 1495 1995 1495
Size: 500 3942
StuI AGG'CCT
Cuts at: 298 298
Size: 4442
StyI C'CwwG_G
Cuts at: 1017 1576 1930 1017
Size: 559 354 3529
Fragments arranged by size:
3529 559 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2025 2158 2617 2826 2025
Size: 133 459 209 3641
Fragments arranged by size:
3641 459 209 133
TatI w'GTAC_w
Cuts at: 1398 3957 1398
Size: 2559 1883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 301 333 973 1005 3959 3991 301
Size: 32 640 32 2954 32 752
Fragments arranged by size:
2954 752 640 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2239 2239
Size: 4442
XbaI T'CTAG_A
Cuts at: 1473 1473
Size: 4442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 535 1050 1937 535
Size: 515 887 3040
Fragments arranged by size:
3040 887 515
XmaI C'CCGG_G
Cuts at: 133 1331 1462 133
Size: 1198 131 3113
Fragments arranged by size:
3113 1198 131
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI