Restriction map for
pF1KB6634
(Circular) (Six-base) MAPSORT of: pF1KB6634.seq Check: 262 from: 1 to: 4433
pF1KB6634 4433 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 759 810 1200 759
Size: 51 390 3992
Fragments arranged by size:
3992 390 51
AccI GT'mk_AC
Cuts at: 1471 1471
Size: 4433
Acc65I G'GTAC_C
Cuts at: 1449 1449
Size: 4433
AclI AA'CG_TT
Cuts at: 1427 4273 1427
Size: 2846 1587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 2258 2690 3437 3705 919
Size: 1339 432 747 268 1647
Fragments arranged by size:
1647 1339 747 432 268
AflIII A'CryG_T
Cuts at: 284 1785 2889 3866 284
Size: 1501 1104 977 851
Fragments arranged by size:
1501 1104 977 851
AgeI A'CCGG_T
Cuts at: 2821 2821
Size: 4433
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 757 1126 757
Size: 369 4064
AlwNI CAG_nnn'CTG
Cuts at: 188 1215 1302 1738 3305 188
Size: 1027 87 436 1567 1316
Fragments arranged by size:
1567 1316 1027 436 87
ApaI G_GGCC'C
Cuts at: 127 127
Size: 4433
ApaLI G'TGCA_C
Cuts at: 3203 3203
Size: 4433
ApoI r'AATT_y
Cuts at: 1437 3786 4042 1437
Size: 2349 256 1828
Fragments arranged by size:
2349 1828 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
AvaI C'yCGr_G
Cuts at: 174 708 1130 1275 1390 1453 174
Size: 534 422 145 115 63 3154
Fragments arranged by size:
3154 534 422 145 115 63
BamHI G'GATC_C
Cuts at: 737 1458 737
Size: 721 3712
BanI G'GyrC_C
Cuts at: 553 712 822 1449 1863 2111 2146 553
Size: 159 110 627 414 248 35 2840
Fragments arranged by size:
2840 627 414 248 159 110 35
BanII G_rGCy'C
Cuts at: 127 1391 1447 2477 127
Size: 1264 56 1030 2083
Fragments arranged by size:
2083 1264 1030 56
BbeI G_GCGC'C
Cuts at: 557 1867 2115 557
Size: 1310 248 2875
Fragments arranged by size:
2875 1310 248
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3092 4433 2331
Size: 761 1341 2331
Fragments arranged by size:
2331 1341 761
BclI T'GATC_A
Cuts at: 1953 1953
Size: 4433
BglI GCCn_nnn'nGGC
Cuts at: 154 802 901 1150 154
Size: 648 99 249 3437
Fragments arranged by size:
3437 648 249 99
BglII A'GATC_T
Cuts at: 1948 3985 1948
Size: 2037 2396
BlpI GC'TnA_GC
Cuts at: 1545 1545
Size: 4433
Bme1580I G_kGCm'C
Cuts at: 127 537 827 2058 2151 3207 127
Size: 410 290 1231 93 1056 1353
Fragments arranged by size:
1353 1231 1056 410 290 93
BmgBI CAC'GTC
Cuts at: 287 317 426 287
Size: 30 109 4294
Fragments arranged by size:
4294 109 30
BmrI ACTGGGnnnn_n'
Cuts at: 1645 1726 2061 4205 1645
Size: 81 335 2144 1873
Fragments arranged by size:
2144 1873 335 81
BmtI G_CTAG'C
Cuts at: 748 748
Size: 4433
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 4433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 579
Size: 4433
Bpu10I CC'TnA_GC
Cuts at: 1198 1263 1198
Size: 65 4368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1609 2488 2980 3278 1609
Size: 879 492 298 2764
Fragments arranged by size:
2764 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4137 4137
Size: 4433
BsaAI yAC'GTr
Cuts at: 1786 1786
Size: 4433
BsaBI GATnn'nnATC
Cuts at: 6 1947 3984 6
Size: 1941 2037 455
Fragments arranged by size:
2037 1941 455
BsaHI Gr'CG_yC
Cuts at: 523 554 682 867 1864 2112 2814 4310 523
Size: 31 128 185 997 248 702 1496 646
Fragments arranged by size:
1496 997 702 646 248 185 128 31
BsaWI w'CCGG_w
Cuts at: 1622 2143 2821 3095 3242 3653 1622
Size: 521 678 274 147 411 2402
Fragments arranged by size:
2402 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 982 1012 982
Size: 30 4403
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1263 1263
Size: 4433
BseYI C'CCAG_C
Cuts at: 188 380 597 612 915 927 1029 1098 1295
Size: 192 217 15 303 12 102 69 197
Cuts at: 1295 1858 2616 3193 3689 188
Size: 563 758 577 496 932
Fragments arranged by size:
932 758 577 563 496 303 217 197
192 102 69 15 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 787 246
Size: 541 3892
BsiEI CG_ry'CG
Cuts at: 104 200 1402 1975 2021 3229 104
Size: 96 1202 573 46 1208 1308
Fragments arranged by size:
1308 1208 1202 573 96 46
BsiHKAI G_wGCw'C
Cuts at: 1447 2225 2415 3207 1447
Size: 778 190 792 2673
Fragments arranged by size:
2673 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 805 805
Size: 4433
Bsp1286I G_dGCh'C
Cuts at: 127 537 827 1391 1447 2058 2151 2225 2415
Size: 410 290 564 56 611 93 74 190
Cuts at: 2415 2477 3207 127
Size: 62 730 1353
Fragments arranged by size:
1353 730 611 564 410 290 190 93
74 62 56
BspEI T'CCGG_A
Cuts at: 3653 3653
Size: 4433
BspHI T'CATG_A
Cuts at: 3609 3609
Size: 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 491 759 810 1094 1200 1483 1697 1999 2380
Size: 268 51 284 106 283 214 302 381
Cuts at: 2380 491
Size: 2544
Fragments arranged by size:
2544 381 302 284 283 268 214 106
51
BsrBI CCG'CTC
Cuts at: 50 571 2725 2779 4263 4428 50
Size: 521 2154 54 1484 165 55
Fragments arranged by size:
2154 1484 521 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2345 2345
Size: 4433
BsrFI r'CCGG_y
Cuts at: 351 787 2431 2612 2821 351
Size: 436 1644 181 209 1963
Fragments arranged by size:
1963 1644 436 209 181
BssHII G'CGCG_C
Cuts at: 391 391
Size: 4433
BssSI C'ACGA_G
Cuts at: 607 2704 3062 607
Size: 2097 358 1978
Fragments arranged by size:
2097 1978 358
BstAPI GCAn_nnn'nTGC
Cuts at: 765 1215 1810 765
Size: 450 595 3388
Fragments arranged by size:
3388 595 450
BstBI TT'CG_AA
Cuts at: 2794 2794
Size: 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 792 792
Size: 4433
BstYI r'GATC_y
Cuts at: 1 737 1458 1925 1948 2283 2529 3541 3627
Size: 736 721 467 23 335 246 1012 86
Cuts at: 3627 3639 3985 1
Size: 12 346 449
Fragments arranged by size:
1012 736 721 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1382 107
Size: 1275 3158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 201 2383 2564 2727 201
Size: 2182 181 163 1907
Fragments arranged by size:
2182 1907 181 163
BtsI GCAGTG_nn'
Cuts at: 1162 1676 1162
Size: 514 3919
DraI TTT'AAA
Cuts at: 1432 1432
Size: 4433
DrdI GACnn_nn'nnGTC
Cuts at: 2139 2997 2139
Size: 858 3575
EaeI y'GGCC_r
Cuts at: 197 233 353 625 639 656 794 965 1399
Size: 36 120 272 14 17 138 171 434
Cuts at: 1399 2018 2192 2583 2610 3955 197
Size: 619 174 391 27 1345 675
Fragments arranged by size:
1345 675 619 434 391 272 174 171
138 120 36 27 17 14
EagI C'GGCC_G
Cuts at: 197 1399 2018 197
Size: 1202 619 2612
Fragments arranged by size:
2612 1202 619
EarI CTCTTCn'nnn_
Cuts at: 316 1015 1078 2456 2666 316
Size: 699 63 1378 210 2083
Fragments arranged by size:
2083 1378 699 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2641 2951 3097 4243 2641
Size: 310 146 1146 2831
Fragments arranged by size:
2831 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1445 1445
Size: 4433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 919 2258 2690 3437 3705 579
Size: 340 1339 432 747 268 1307
Fragments arranged by size:
1339 1307 747 432 340 268
EcoNI CCTnn'n_nnAGG
Cuts at: 732 1207 732
Size: 475 3958
EcoO109I rG'GnC_Cy
Cuts at: 830 894 1326 1572 830
Size: 64 432 246 3691
Fragments arranged by size:
3691 432 246 64
EcoRI G'AATT_C
Cuts at: 1437 1437
Size: 4433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4433
FspI TGC'GCA
Cuts at: 280 517 1168 2214 280
Size: 237 651 1046 2499
Fragments arranged by size:
2499 1046 651 237
HaeII r_GCGC'y
Cuts at: 381 443 557 1099 1140 1867 2115 3137 4116
Size: 62 114 542 41 727 248 1022 979
Cuts at: 4116 381
Size: 698
Fragments arranged by size:
1022 979 727 698 542 248 114 62
41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 982 1014 1234 1246 1266 1278 2275 2305 2307
Size: 32 220 12 20 12 997 30 2
Cuts at: 2307 2337 982
Size: 30 3078
Fragments arranged by size:
3078 997 220 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 1472 1472
Size: 4433
KasI G'GCGC_C
Cuts at: 553 1863 2111 553
Size: 1310 248 2875
Fragments arranged by size:
2875 1310 248
KpnI G_GTAC'C
Cuts at: 1453 1453
Size: 4433
MluI A'CGCG_T
Cuts at: 3866 3866
Size: 4433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 3104 3288 314
Size: 2790 184 1459
Fragments arranged by size:
2790 1459 184
MscI TGG'CCA
Cuts at: 641 658 796 967 2194 641
Size: 17 138 171 1227 2880
Fragments arranged by size:
2880 1227 171 138 17
MslI CAynn'nnrTG
Cuts at: 790 2422 2549 790
Size: 1632 127 2674
Fragments arranged by size:
2674 1632 127
MspA1I CmG'CkG
Cuts at: 185 501 1181 1221 1299 1335 1351 1384 1544
Size: 316 680 40 78 36 16 33 160
Cuts at: 1544 1858 2218 3231 3476 185
Size: 314 360 1013 245 1142
Fragments arranged by size:
1142 1013 680 360 316 314 245 160
78 40 36 33 16
NaeI GCC'GGC
Cuts at: 353 2614 353
Size: 2261 2172
NarI GG'CG_CC
Cuts at: 554 1864 2112 554
Size: 1310 248 2875
Fragments arranged by size:
2875 1310 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4433
NdeI CA'TA_TG
Cuts at: 718 718
Size: 4433
NgoMIV G'CCGG_C
Cuts at: 351 2612 351
Size: 2261 2172
NheI G'CTAG_C
Cuts at: 744 744
Size: 4433
NotI GC'GGCC_GC
Cuts at: 197 1399 197
Size: 1202 3231
NspI r_CATG'y
Cuts at: 936 1486 1986 2893 936
Size: 550 500 907 2476
Fragments arranged by size:
2476 907 550 500
PasI CC'CwG_GG
Cuts at: 841 841
Size: 4433
PciI A'CATG_T
Cuts at: 2889 2889
Size: 4433
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4401
PpuMI rG'GwC_Cy
Cuts at: 830 1326 830
Size: 496 3937
PspOMI G'GGCC_C
Cuts at: 123 123
Size: 4433
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 355 387 355
Size: 32 4401
PstI C_TGCA'G
Cuts at: 439 736 821 1480 2165 439
Size: 297 85 659 685 2707
Fragments arranged by size:
2707 685 659 297 85
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4433
PvuII CAG'CTG
Cuts at: 1299 1858 2218 1299
Size: 559 360 3514
Fragments arranged by size:
3514 559 360
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4433
SacI G_AGCT'C
Cuts at: 1447 1447
Size: 4433
SacII CC_GC'GG
Cuts at: 1385 1385
Size: 4433
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4433
SapI GCTCTTCn'nnn_
Cuts at: 316 2456 2666 316
Size: 2140 210 2083
Fragments arranged by size:
2140 2083 210
SbfI CC_TGCA'GG
Cuts at: 1480 1480
Size: 4433
ScaI AGT'ACT
Cuts at: 3950 3950
Size: 4433
SexAI A'CCwGG_T
Cuts at: 946 946
Size: 4433
SfcI C'TryA_G
Cuts at: 33 435 732 817 1204 1476 2161 3154 3345
Size: 402 297 85 387 272 685 993 191
Cuts at: 3345 33
Size: 1121
Fragments arranged by size:
1121 993 685 402 387 297 272 191
85
SfiI GGCCn_nnn'nGGCC
Cuts at: 802 901 1150 802
Size: 99 249 4085
Fragments arranged by size:
4085 249 99
SfoI GGC'GCC
Cuts at: 555 1865 2113 555
Size: 1310 248 2875
Fragments arranged by size:
2875 1310 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
SmaI CCC'GGG
Cuts at: 176 1132 1455 176
Size: 956 323 3154
Fragments arranged by size:
3154 956 323
SmlI C'TyrA_G
Cuts at: 1588 2503 2995 3257 1588
Size: 915 492 262 2764
Fragments arranged by size:
2764 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4433
SphI G_CATG'C
Cuts at: 1486 1986 1486
Size: 500 3933
StuI AGG'CCT
Cuts at: 805 1007 805
Size: 202 4231
StyI C'CwwG_G
Cuts at: 107 1567 1921 107
Size: 1460 354 2619
Fragments arranged by size:
2619 1460 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 180 372 1336 2016 2149 2608 2817 180
Size: 192 964 680 133 459 209 1796
Fragments arranged by size:
1796 964 680 459 209 192 133
TatI w'GTAC_w
Cuts at: 3948 3948
Size: 4433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1400 1432 3950 3982 1400
Size: 32 2518 32 1851
Fragments arranged by size:
2518 1851 32 32
Tth111I GACn'n_nGTC
Cuts at: 2230 2230
Size: 4433
XbaI T'CTAG_A
Cuts at: 1464 1464
Size: 4433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 297 912 1928 297
Size: 615 1016 2802
Fragments arranged by size:
2802 1016 615
XmaI C'CCGG_G
Cuts at: 174 1130 1453 174
Size: 956 323 3154
Fragments arranged by size:
3154 956 323
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BsiWI BsmI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspXI SanDI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI