Restriction map for
pF1KB6632
(Circular) (Six-base) MAPSORT of: pF1KB6632.seq Check: 6774 from: 1 to: 4433
pF1KB6632 4433 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 429 483 1262 429
Size: 54 779 3600
Fragments arranged by size:
3600 779 54
AccI GT'mk_AC
Cuts at: 1471 1471
Size: 4433
Acc65I G'GTAC_C
Cuts at: 1449 1449
Size: 4433
AclI AA'CG_TT
Cuts at: 4273 4273
Size: 4433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 970 2258 2690 3437 3705 292
Size: 678 1288 432 747 268 1020
Fragments arranged by size:
1288 1020 747 678 432 268
AfeI AGC'GCT
Cuts at: 126 126
Size: 4433
AflIII A'CryG_T
Cuts at: 371 1034 1785 2889 3866 371
Size: 663 751 1104 977 938
Fragments arranged by size:
1104 977 938 751 663
AgeI A'CCGG_T
Cuts at: 2821 2821
Size: 4433
AlwNI CAG_nnn'CTG
Cuts at: 952 1738 3305 952
Size: 786 1567 2080
Fragments arranged by size:
2080 1567 786
ApaLI G'TGCA_C
Cuts at: 3203 3203
Size: 4433
ApoI r'AATT_y
Cuts at: 1043 1437 3786 4042 1043
Size: 394 2349 256 1434
Fragments arranged by size:
2349 1434 394 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
AvaI C'yCGr_G
Cuts at: 243 820 1453 243
Size: 577 633 3223
Fragments arranged by size:
3223 633 577
BamHI G'GATC_C
Cuts at: 710 906 1458 710
Size: 196 552 3685
Fragments arranged by size:
3685 552 196
BanI G'GyrC_C
Cuts at: 145 360 397 751 781 885 1231 1449 1863
Size: 215 37 354 30 104 346 218 414
Cuts at: 1863 2111 2146 145
Size: 248 35 2432
Fragments arranged by size:
2432 414 354 346 248 218 215 104
37 35 30
BanII G_rGCy'C
Cuts at: 212 1447 2477 212
Size: 1235 1030 2168
Fragments arranged by size:
2168 1235 1030
BbeI G_GCGC'C
Cuts at: 149 1867 2115 149
Size: 1718 248 2467
Fragments arranged by size:
2467 1718 248
BbsI GAAGACnn'nnnn_
Cuts at: 337 337
Size: 4433
BbvCI CC'TCA_GC
Cuts at: 1259 1340 1388 1259
Size: 81 48 4304
Fragments arranged by size:
4304 81 48
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3092 4433 2331
Size: 761 1341 2331
Fragments arranged by size:
2331 1341 761
BclI T'GATC_A
Cuts at: 1953 1953
Size: 4433
BglI GCCn_nnn'nGGC
Cuts at: 196 196
Size: 4433
BglII A'GATC_T
Cuts at: 1412 1948 3985 1412
Size: 536 2037 1860
Fragments arranged by size:
2037 1860 536
BlpI GC'TnA_GC
Cuts at: 128 1545 128
Size: 1417 3016
Bme1580I G_kGCm'C
Cuts at: 242 365 786 1236 2058 2151 3207 242
Size: 123 421 450 822 93 1056 1468
Fragments arranged by size:
1468 1056 822 450 421 123 93
BmgBI CAC'GTC
Cuts at: 1035 1035
Size: 4433
BmrI ACTGGGnnnn_n'
Cuts at: 403 1385 1645 1726 2061 4205 403
Size: 982 260 81 335 2144 631
Fragments arranged by size:
2144 982 631 335 260 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 4433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 334 520 859 929 983 1134 226
Size: 108 186 339 70 54 151 3525
Fragments arranged by size:
3525 339 186 151 108 70 54
Bpu10I CC'TnA_GC
Cuts at: 1259 1334 1340 1388 1259
Size: 75 6 48 4304
Fragments arranged by size:
4304 75 48 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1609 2488 2980 3278 1609
Size: 879 492 298 2764
Fragments arranged by size:
2764 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1215 4137 1215
Size: 2922 1511
BsaAI yAC'GTr
Cuts at: 1786 1786
Size: 4433
BsaBI GATnn'nnATC
Cuts at: 6 1947 3984 6
Size: 1941 2037 455
Fragments arranged by size:
2037 1941 455
BsaHI Gr'CG_yC
Cuts at: 119 146 1864 2112 2814 4310 119
Size: 27 1718 248 702 1496 242
Fragments arranged by size:
1718 1496 702 248 242 27
BsaWI w'CCGG_w
Cuts at: 707 1622 2143 2821 3095 3242 3653 707
Size: 915 521 678 274 147 411 1487
Fragments arranged by size:
1487 915 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 627 657 1132 1162 627
Size: 30 475 30 3898
Fragments arranged by size:
3898 475 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 885 971 1357 885
Size: 86 386 3961
Fragments arranged by size:
3961 386 86
BseYI C'CCAG_C
Cuts at: 186 191 364 727 931 1081 1858 2616 3193
Size: 5 173 363 204 150 777 758 577
Cuts at: 3193 3689 186
Size: 496 930
Fragments arranged by size:
930 777 758 577 496 363 204 173
150 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 1024 193
Size: 831 3602
BsiEI CG_ry'CG
Cuts at: 104 1975 2021 3229 104
Size: 1871 46 1208 1308
Fragments arranged by size:
1871 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 945 1447 2225 2415 3207 945
Size: 502 778 190 792 2171
Fragments arranged by size:
2171 792 778 502 190
Bsp1286I G_dGCh'C
Cuts at: 212 242 365 786 945 1236 1447 2058 2151
Size: 30 123 421 159 291 211 611 93
Cuts at: 2151 2225 2415 2477 3207 212
Size: 74 190 62 730 1438
Fragments arranged by size:
1438 730 611 421 291 211 190 159
123 93 74 62 30
BspEI T'CCGG_A
Cuts at: 3653 3653
Size: 4433
BspHI T'CATG_A
Cuts at: 573 1164 3609 573
Size: 591 2445 1397
Fragments arranged by size:
2445 1397 591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 429 483 1262 1483 1697 1999 2380 429
Size: 54 779 221 214 302 381 2482
Fragments arranged by size:
2482 779 381 302 221 214 54
BsrBI CCG'CTC
Cuts at: 50 1170 2725 2779 4263 4428 50
Size: 1120 1555 54 1484 165 55
Fragments arranged by size:
1555 1484 1120 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1071 2345 1071
Size: 1274 3159
BsrFI r'CCGG_y
Cuts at: 487 2431 2612 2821 487
Size: 1944 181 209 2099
Fragments arranged by size:
2099 1944 209 181
BssSI C'ACGA_G
Cuts at: 2704 3062 2704
Size: 358 4075
BstAPI GCAn_nnn'nTGC
Cuts at: 1810 1810
Size: 4433
BstBI TT'CG_AA
Cuts at: 2794 2794
Size: 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4433
BstYI r'GATC_y
Cuts at: 1 710 906 1412 1458 1925 1948 2283 2529
Size: 709 196 506 46 467 23 335 246
Cuts at: 2529 3541 3627 3639 3985 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 709 506 467 449 346 335 246
196 86 46 23 12
Bsu36I CC'TnA_GG
Cuts at: 1128 1128
Size: 4433
BtgI C'CryG_G
Cuts at: 107 165 899 107
Size: 58 734 3641
Fragments arranged by size:
3641 734 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2383 2564 2727 2383
Size: 181 163 4089
Fragments arranged by size:
4089 181 163
BtsI GCAGTG_nn'
Cuts at: 1676 1676
Size: 4433
DraI TTT'AAA
Cuts at: 1432 1432
Size: 4433
DraIII CAC_nnn'GTG
Cuts at: 171 781 171
Size: 610 3823
DrdI GACnn_nn'nnGTC
Cuts at: 2139 2997 2139
Size: 858 3575
EaeI y'GGCC_r
Cuts at: 381 2018 2192 2583 2610 3955 381
Size: 1637 174 391 27 1345 859
Fragments arranged by size:
1637 1345 859 391 174 27
EagI C'GGCC_G
Cuts at: 2018 2018
Size: 4433
EarI CTCTTCn'nnn_
Cuts at: 989 1098 1274 2456 2666 989
Size: 109 176 1182 210 2756
Fragments arranged by size:
2756 1182 210 176 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1261 1305 2641 2951 3097 4243 1261
Size: 44 1336 310 146 1146 1451
Fragments arranged by size:
1451 1336 1146 310 146 44
EcoICRI GAG'CTC
Cuts at: 1445 1445
Size: 4433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 292 334 520 859 929 970 983 1134
Size: 66 42 186 339 70 41 13 151
Cuts at: 1134 2258 2690 3437 3705 226
Size: 1124 432 747 268 954
Fragments arranged by size:
1124 954 747 432 339 268 186 151
70 66 42 41 13
EcoNI CCTnn'n_nnAGG
Cuts at: 880 880
Size: 4433
EcoO109I rG'GnC_Cy
Cuts at: 387 873 1365 1572 387
Size: 486 492 207 3248
Fragments arranged by size:
3248 492 486 207
EcoRI G'AATT_C
Cuts at: 1437 1437
Size: 4433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4433
FspI TGC'GCA
Cuts at: 2214 2214
Size: 4433
HaeII r_GCGC'y
Cuts at: 128 149 1867 2115 3137 4116 128
Size: 21 1718 248 1022 979 445
Fragments arranged by size:
1718 1022 979 445 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 627 659 702 734 1132 1164 1297 1329 2275
Size: 32 43 32 398 32 133 32 946
Cuts at: 2275 2305 2307 2337 627
Size: 30 2 30 2723
Fragments arranged by size:
2723 946 398 133 43 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1472 1472
Size: 4433
KasI G'GCGC_C
Cuts at: 145 1863 2111 145
Size: 1718 248 2467
Fragments arranged by size:
2467 1718 248
KpnI G_GTAC'C
Cuts at: 1453 1453
Size: 4433
MluI A'CGCG_T
Cuts at: 3866 3866
Size: 4433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3104 3288 3104
Size: 184 4249
MscI TGG'CCA
Cuts at: 2194 2194
Size: 4433
MslI CAynn'nnrTG
Cuts at: 340 2422 2549 340
Size: 2082 127 2224
Fragments arranged by size:
2224 2082 127
MspA1I CmG'CkG
Cuts at: 1117 1263 1544 1858 2218 3231 3476 1117
Size: 146 281 314 360 1013 245 2074
Fragments arranged by size:
2074 1013 360 314 281 245 146
NaeI GCC'GGC
Cuts at: 489 2614 489
Size: 2125 2308
NarI GG'CG_CC
Cuts at: 146 1864 2112 146
Size: 1718 248 2467
Fragments arranged by size:
2467 1718 248
NcoI C'CATG_G
Cuts at: 107 899 107
Size: 792 3641
NgoMIV G'CCGG_C
Cuts at: 487 2612 487
Size: 2125 2308
NspI r_CATG'y
Cuts at: 375 451 1486 1986 2893 375
Size: 76 1035 500 907 1915
Fragments arranged by size:
1915 1035 907 500 76
PciI A'CATG_T
Cuts at: 371 2889 371
Size: 2518 1915
PflMI CCAn_nnn'nTGG
Cuts at: 972 1076 1306 972
Size: 104 230 4099
Fragments arranged by size:
4099 230 104
PfoI T'CCnGG_A
Cuts at: 536 1028 1217 536
Size: 492 189 3752
Fragments arranged by size:
3752 492 189
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4401
PspXI vC'TCGA_Gb
Cuts at: 243 243
Size: 4433
PstI C_TGCA'G
Cuts at: 418 884 1293 1480 2165 418
Size: 466 409 187 685 2686
Fragments arranged by size:
2686 685 466 409 187
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4433
PvuII CAG'CTG
Cuts at: 1117 1263 1858 2218 1117
Size: 146 595 360 3332
Fragments arranged by size:
3332 595 360 146
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4433
SacI G_AGCT'C
Cuts at: 1447 1447
Size: 4433
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4433
SapI GCTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4223
SbfI CC_TGCA'GG
Cuts at: 1480 1480
Size: 4433
ScaI AGT'ACT
Cuts at: 3950 3950
Size: 4433
SfcI C'TryA_G
Cuts at: 33 414 880 1289 1476 2161 3154 3345 33
Size: 381 466 409 187 685 993 191 1121
Fragments arranged by size:
1121 993 685 466 409 381 191 187
SfoI GGC'GCC
Cuts at: 147 1865 2113 147
Size: 1718 248 2467
Fragments arranged by size:
2467 1718 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
SmaI CCC'GGG
Cuts at: 1455 1455
Size: 4433
SmlI C'TyrA_G
Cuts at: 243 1588 2503 2995 3257 243
Size: 1345 915 492 262 1419
Fragments arranged by size:
1419 1345 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4433
SphI G_CATG'C
Cuts at: 1486 1986 1486
Size: 500 3933
StyI C'CwwG_G
Cuts at: 107 899 1567 1921 107
Size: 792 668 354 2619
Fragments arranged by size:
2619 792 668 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 385 2016 2149 2608 2817 385
Size: 1631 133 459 209 2001
Fragments arranged by size:
2001 1631 459 209 133
TatI w'GTAC_w
Cuts at: 3948 3948
Size: 4433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3950 3982 3950
Size: 32 4401
Tth111I GACn'n_nGTC
Cuts at: 2230 2230
Size: 4433
XbaI T'CTAG_A
Cuts at: 1464 1464
Size: 4433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1928 1928
Size: 4433
XhoI C'TCGA_G
Cuts at: 243 243
Size: 4433
XmaI C'CCGG_G
Cuts at: 1453 1453
Size: 4433
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI