Restriction map for
pF1KB6623
(Circular) (Six-base) MAPSORT of: pF1KB6623.seq Check: 5740 from: 1 to: 4352
pF1KB6623 4352 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1390 1390
Size: 4352
Acc65I G'GTAC_C
Cuts at: 1368 1368
Size: 4352
AclI AA'CG_TT
Cuts at: 4192 4192
Size: 4352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 421 2177 2609 3356 3624 236
Size: 185 1756 432 747 268 964
Fragments arranged by size:
1756 964 747 432 268 185
AflIII A'CryG_T
Cuts at: 1704 2808 3785 1704
Size: 1104 977 2271
Fragments arranged by size:
2271 1104 977
AgeI A'CCGG_T
Cuts at: 2740 2740
Size: 4352
AlwNI CAG_nnn'CTG
Cuts at: 1657 3224 1657
Size: 1567 2785
ApaLI G'TGCA_C
Cuts at: 3122 3122
Size: 4352
ApoI r'AATT_y
Cuts at: 183 892 1356 3705 3961 183
Size: 709 464 2349 256 574
Fragments arranged by size:
2349 709 574 464 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4352
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
AvaI C'yCGr_G
Cuts at: 1372 1372
Size: 4352
BamHI G'GATC_C
Cuts at: 1377 1377
Size: 4352
BanI G'GyrC_C
Cuts at: 1368 1782 2030 2065 1368
Size: 414 248 35 3655
Fragments arranged by size:
3655 414 248 35
BanII G_rGCy'C
Cuts at: 153 251 454 1366 2396 153
Size: 98 203 912 1030 2109
Fragments arranged by size:
2109 1030 912 203 98
BbeI G_GCGC'C
Cuts at: 1786 2034 1786
Size: 248 4104
BbsI GAAGACnn'nnnn_
Cuts at: 439 706 853 935 1152 1268 439
Size: 267 147 82 217 116 3523
Fragments arranged by size:
3523 267 217 147 116 82
BciVI GTATCCnnnnn_n'
Cuts at: 151 270 2250 3011 4352 151
Size: 119 1980 761 1341 151
Fragments arranged by size:
1980 1341 761 151 119
BclI T'GATC_A
Cuts at: 1872 1872
Size: 4352
BglI GCCn_nnn'nGGC
Cuts at: 334 334
Size: 4352
BglII A'GATC_T
Cuts at: 1867 3904 1867
Size: 2037 2315
BlpI GC'TnA_GC
Cuts at: 1464 1464
Size: 4352
Bme1580I G_kGCm'C
Cuts at: 1171 1977 2070 3126 1171
Size: 806 93 1056 2397
Fragments arranged by size:
2397 1056 806 93
BmgBI CAC'GTC
Cuts at: 273 273
Size: 4352
BmrI ACTGGGnnnn_n'
Cuts at: 312 1564 1645 1980 4124 312
Size: 1252 81 335 2144 540
Fragments arranged by size:
2144 1252 540 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1292 2719 1292
Size: 1427 2925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 4352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1009 1528 2407 2899 3197 985
Size: 24 519 879 492 298 2140
Fragments arranged by size:
2140 879 519 492 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 4056 4056
Size: 4352
BsaAI yAC'GTr
Cuts at: 1705 1705
Size: 4352
BsaBI GATnn'nnATC
Cuts at: 6 1866 3903 6
Size: 1860 2037 455
Fragments arranged by size:
2037 1860 455
BsaHI Gr'CG_yC
Cuts at: 1783 2031 2733 4229 1783
Size: 248 702 1496 1906
Fragments arranged by size:
1906 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1307 1541 2062 2740 3014 3161 3572 1307
Size: 234 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 234 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1225 1225
Size: 4352
BseYI C'CCAG_C
Cuts at: 1777 2535 3112 3608 1777
Size: 758 577 496 2521
Fragments arranged by size:
2521 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1894 1940 3148 104
Size: 1790 46 1208 1308
Fragments arranged by size:
1790 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1366 2144 2334 3126 1366
Size: 778 190 792 2592
Fragments arranged by size:
2592 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 153 251 454 1171 1366 1977 2070 2144 2334
Size: 98 203 717 195 611 93 74 190
Cuts at: 2334 2396 3126 153
Size: 62 730 1379
Fragments arranged by size:
1379 730 717 611 203 195 190 98
93 74 62
BspEI T'CCGG_A
Cuts at: 1307 3572 1307
Size: 2265 2087
BspHI T'CATG_A
Cuts at: 3528 3528
Size: 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 719 1402 1616 1918 2299 719
Size: 683 214 302 381 2772
Fragments arranged by size:
2772 683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2644 2698 4182 4347 50
Size: 2594 54 1484 165 55
Fragments arranged by size:
2594 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 620 2264 620
Size: 1644 2708
BsrFI r'CCGG_y
Cuts at: 2350 2531 2740 2350
Size: 181 209 3962
Fragments arranged by size:
3962 209 181
BssSI C'ACGA_G
Cuts at: 2623 2981 2623
Size: 358 3994
BstAPI GCAn_nnn'nTGC
Cuts at: 1729 1729
Size: 4352
BstBI TT'CG_AA
Cuts at: 2713 2713
Size: 4352
BstXI CCAn_nnnn'nTGG
Cuts at: 444 633 444
Size: 189 4163
BstYI r'GATC_y
Cuts at: 1 1377 1844 1867 2202 2448 3460 3546 3558
Size: 1376 467 23 335 246 1012 86 12
Cuts at: 3558 3904 1
Size: 346 449
Fragments arranged by size:
1376 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1124 1124
Size: 4352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2302 2483 2646 2302
Size: 181 163 4008
Fragments arranged by size:
4008 181 163
BtsI GCAGTG_nn'
Cuts at: 1595 1595
Size: 4352
DraI TTT'AAA
Cuts at: 1351 1351
Size: 4352
DrdI GACnn_nn'nnGTC
Cuts at: 2058 2916 2058
Size: 858 3494
EaeI y'GGCC_r
Cuts at: 326 1937 2111 2502 2529 3874 326
Size: 1611 174 391 27 1345 804
Fragments arranged by size:
1611 1345 804 391 174 27
EagI C'GGCC_G
Cuts at: 1937 1937
Size: 4352
EarI CTCTTCn'nnn_
Cuts at: 1004 2375 2585 1004
Size: 1371 210 2771
Fragments arranged by size:
2771 1371 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2560 2870 3016 4162 2560
Size: 310 146 1146 2750
Fragments arranged by size:
2750 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1364 1364
Size: 4352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 236 421 907 2177 2609 3356 3624 236
Size: 185 486 1270 432 747 268 964
Fragments arranged by size:
1270 964 747 486 432 268 185
EcoO109I rG'GnC_Cy
Cuts at: 1491 1491
Size: 4352
EcoRI G'AATT_C
Cuts at: 1356 1356
Size: 4352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4352
FspI TGC'GCA
Cuts at: 2133 2133
Size: 4352
HaeII r_GCGC'y
Cuts at: 1020 1786 2034 3056 4035 1020
Size: 766 248 1022 979 1337
Fragments arranged by size:
1337 1022 979 766 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 1060 1092 2194 2224 2226 2256 484
Size: 32 544 32 1102 30 2 30 2580
Fragments arranged by size:
2580 1102 544 32 32 30 30 2
HincII GTy'rAC
Cuts at: 477 1391 477
Size: 914 3438
HindIII A'AGCT_T
Cuts at: 361 361
Size: 4352
KasI G'GCGC_C
Cuts at: 1782 2030 1782
Size: 248 4104
KpnI G_GTAC'C
Cuts at: 1372 1372
Size: 4352
MfeI C'AATT_G
Cuts at: 311 311
Size: 4352
MluI A'CGCG_T
Cuts at: 3785 3785
Size: 4352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1103 3023 3207 1070
Size: 33 1920 184 2215
Fragments arranged by size:
2215 1920 184 33
MscI TGG'CCA
Cuts at: 328 2113 328
Size: 1785 2567
MslI CAynn'nnrTG
Cuts at: 139 1129 2341 2468 139
Size: 990 1212 127 2023
Fragments arranged by size:
2023 1212 990 127
MspA1I CmG'CkG
Cuts at: 1463 1777 2137 3150 3395 1463
Size: 314 360 1013 245 2420
Fragments arranged by size:
2420 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2533 2533
Size: 4352
NarI GG'CG_CC
Cuts at: 1783 2031 1783
Size: 248 4104
NcoI C'CATG_G
Cuts at: 1124 1124
Size: 4352
NgoMIV G'CCGG_C
Cuts at: 2531 2531
Size: 4352
NspI r_CATG'y
Cuts at: 1405 1905 2812 1405
Size: 500 907 2945
Fragments arranged by size:
2945 907 500
PasI CC'CwG_GG
Cuts at: 153 153
Size: 4352
PciI A'CATG_T
Cuts at: 2808 2808
Size: 4352
PflMI CCAn_nnn'nTGG
Cuts at: 324 324
Size: 4352
PfoI T'CCnGG_A
Cuts at: 1061 1061
Size: 4352
PmeI GTTT'AAAC
Cuts at: 1351 1351
Size: 4352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 4320
PsiI TTA'TAA
Cuts at: 422 422
Size: 4352
PstI C_TGCA'G
Cuts at: 812 1399 2084 812
Size: 587 685 3080
Fragments arranged by size:
3080 685 587
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4352
PvuII CAG'CTG
Cuts at: 1777 2137 1777
Size: 360 3992
RsrII CG'GwC_CG
Cuts at: 2547 2547
Size: 4352
SacI G_AGCT'C
Cuts at: 1366 1366
Size: 4352
SalI G'TCGA_C
Cuts at: 1389 1389
Size: 4352
SapI GCTCTTCn'nnn_
Cuts at: 2375 2585 2375
Size: 210 4142
SbfI CC_TGCA'GG
Cuts at: 1399 1399
Size: 4352
ScaI AGT'ACT
Cuts at: 3869 3869
Size: 4352
SfcI C'TryA_G
Cuts at: 33 808 1395 2080 3073 3264 33
Size: 775 587 685 993 191 1121
Fragments arranged by size:
1121 993 775 685 587 191
SfoI GGC'GCC
Cuts at: 1784 2032 1784
Size: 248 4104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
SmaI CCC'GGG
Cuts at: 1374 1374
Size: 4352
SmlI C'TyrA_G
Cuts at: 1000 1024 1507 2422 2914 3176 1000
Size: 24 483 915 492 262 2176
Fragments arranged by size:
2176 915 492 483 262 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4352
SphI G_CATG'C
Cuts at: 1405 1905 1405
Size: 500 3852
StyI C'CwwG_G
Cuts at: 332 437 1124 1486 1840 332
Size: 105 687 362 354 2844
Fragments arranged by size:
2844 687 362 354 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 210 305 1935 2068 2527 2736 210
Size: 95 1630 133 459 209 1826
Fragments arranged by size:
1826 1630 459 209 133 95
TatI w'GTAC_w
Cuts at: 3867 3867
Size: 4352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4320
Tth111I GACn'n_nGTC
Cuts at: 2149 2149
Size: 4352
XbaI T'CTAG_A
Cuts at: 1383 1383
Size: 4352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1847 1847
Size: 4352
XmaI C'CCGG_G
Cuts at: 1372 1372
Size: 4352
XmnI GAAnn'nnTTC
Cuts at: 221 221
Size: 4352
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI