Restriction map for
pF1KB6617
(Circular) (Six-base) MAPSORT of: pF1KB6617.seq Check: 8864 from: 1 to: 4349
pF1KB6617 4349 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 177 448 951 177
Size: 271 503 3575
Fragments arranged by size:
3575 503 271
AccI GT'mk_AC
Cuts at: 1387 1387
Size: 4349
Acc65I G'GTAC_C
Cuts at: 448 1365 448
Size: 917 3432
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 733 2174 2606 3353 3621 316
Size: 417 1441 432 747 268 1044
Fragments arranged by size:
1441 1044 747 432 417 268
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1144 1144
Size: 4349
AlwNI CAG_nnn'CTG
Cuts at: 972 1050 1654 3221 972
Size: 78 604 1567 2100
Fragments arranged by size:
2100 1567 604 78
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 1353 3702 3958 1353
Size: 2349 256 1744
Fragments arranged by size:
2349 1744 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 326 589 1369 326
Size: 263 780 3306
Fragments arranged by size:
3306 780 263
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 448 460 1274 1365 1779 2027 2062 448
Size: 12 814 91 414 248 35 2735
Fragments arranged by size:
2735 814 414 248 91 35 12
BanII G_rGCy'C
Cuts at: 149 217 1044 1190 1363 2393 149
Size: 68 827 146 173 1030 2105
Fragments arranged by size:
2105 1030 827 173 146 68
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbsI GAAGACnn'nnnn_
Cuts at: 513 513
Size: 4349
BbvCI CC'TCA_GC
Cuts at: 514 514
Size: 4349
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 764 773 1006 1205 764
Size: 9 233 199 3908
Fragments arranged by size:
3908 233 199 9
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 1075 1461 1075
Size: 386 3963
Bme1580I G_kGCm'C
Cuts at: 465 1974 2067 3123 465
Size: 1509 93 1056 1691
Fragments arranged by size:
1691 1509 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1561 1642 1977 4121 1561
Size: 81 335 2144 1789
Fragments arranged by size:
2144 1789 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 231 2716 231
Size: 2485 1864
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 226 355 376 556 726 913 133
Size: 93 129 21 180 170 187 3569
Fragments arranged by size:
3569 187 180 170 129 93 21
Bpu10I CC'TnA_GC
Cuts at: 487 514 487
Size: 27 4322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1525 2404 2896 3194 1069
Size: 456 879 492 298 2224
Fragments arranged by size:
2224 879 492 456 298
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 581 1780 2028 2730 4226 581
Size: 1199 248 702 1496 704
Fragments arranged by size:
1496 1199 704 702 248
BsaWI w'CCGG_w
Cuts at: 875 1538 2059 2737 3011 3158 3569 875
Size: 663 521 678 274 147 411 1655
Fragments arranged by size:
1655 678 663 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 464 494 464
Size: 30 4319
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 811 1072 1192 811
Size: 261 120 3968
Fragments arranged by size:
3968 261 120
BseYI C'CCAG_C
Cuts at: 243 464 762 975 992 1774 2532 3109 3605
Size: 221 298 213 17 782 758 577 496
Cuts at: 3605 243
Size: 987
Fragments arranged by size:
987 782 758 577 496 298 221 213
17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1075 985
Size: 90 4259
BsiEI CG_ry'CG
Cuts at: 104 1891 1937 3145 104
Size: 1787 46 1208 1308
Fragments arranged by size:
1787 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 217 1190 1363 2141 2331 3123 217
Size: 973 173 778 190 792 1443
Fragments arranged by size:
1443 973 792 778 190 173
BsmBI CGTCTCn'nnnn_
Cuts at: 1174 1174
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 149 217 465 1044 1190 1363 1974 2067 2141
Size: 68 248 579 146 173 611 93 74
Cuts at: 2141 2331 2393 3123 149
Size: 190 62 730 1375
Fragments arranged by size:
1375 730 611 579 248 190 173 146
93 74 68 62
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 932 3525 932
Size: 2593 1756
BspMI ACCTGCnnnn'nnnn_
Cuts at: 177 439 448 951 1399 1613 1915 2296 177
Size: 262 9 503 448 214 302 381 2230
Fragments arranged by size:
2230 503 448 381 302 262 214 9
BsrBI CCG'CTC
Cuts at: 50 1319 2641 2695 4179 4344 50
Size: 1269 1322 54 1484 165 55
Fragments arranged by size:
1484 1322 1269 165 55 54
BsrDI GCAATG_nn'
Cuts at: 181 504 2261 181
Size: 323 1757 2269
Fragments arranged by size:
2269 1757 323
BsrFI r'CCGG_y
Cuts at: 830 1232 2347 2528 2737 830
Size: 402 1115 181 209 2442
Fragments arranged by size:
2442 1115 402 209 181
BssSI C'ACGA_G
Cuts at: 1165 2620 2978 1165
Size: 1455 358 2536
Fragments arranged by size:
2536 1455 358
BstAPI GCAn_nnn'nTGC
Cuts at: 431 972 1726 431
Size: 541 754 3054
Fragments arranged by size:
3054 754 541
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstYI r'GATC_y
Cuts at: 1 1047 1374 1841 1864 2199 2445 3457 3543
Size: 1046 327 467 23 335 246 1012 86
Cuts at: 3543 3555 3901 1
Size: 12 346 449
Fragments arranged by size:
1046 1012 467 449 346 335 327 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1270 1270
Size: 4349
BtgI C'CryG_G
Cuts at: 107 746 782 882 107
Size: 639 36 100 3574
Fragments arranged by size:
3574 639 100 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2299 2480 2643 2299
Size: 181 163 4005
Fragments arranged by size:
4005 181 163
BtsI GCAGTG_nn'
Cuts at: 783 835 1592 783
Size: 52 757 3540
Fragments arranged by size:
3540 757 52
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DraIII CAC_nnn'GTG
Cuts at: 1155 1155
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 743 832 983 998 1052 1934 2108 2499 2526
Size: 89 151 15 54 882 174 391 27
Cuts at: 2526 3871 743
Size: 1345 1221
Fragments arranged by size:
1345 1221 882 391 174 151 89 54
27 15
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 205 2372 2582 205
Size: 2167 210 1972
Fragments arranged by size:
2167 1972 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 909 2557 2867 3013 4159 909
Size: 1648 310 146 1146 1099
Fragments arranged by size:
1648 1146 1099 310 146
EcoICRI GAG'CTC
Cuts at: 215 1188 1361 215
Size: 973 173 3203
Fragments arranged by size:
3203 973 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 226 316 355 376 556 726 733 913
Size: 93 90 39 21 180 170 7 180
Cuts at: 913 2174 2606 3353 3621 133
Size: 1261 432 747 268 861
Fragments arranged by size:
1261 861 747 432 268 180 180 170
93 90 39 21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 906 958 906
Size: 52 4297
EcoO109I rG'GnC_Cy
Cuts at: 531 552 888 1242 1292 1488 531
Size: 21 336 354 50 196 3392
Fragments arranged by size:
3392 354 336 196 50 21
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1353 2368 1353
Size: 1015 3334
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 234 847 973 1012 1783 2031 3053 4032 234
Size: 613 126 39 771 248 1022 979 551
Fragments arranged by size:
1022 979 771 613 551 248 126 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 464 496 2191 2221 2223 2253 464
Size: 32 1695 30 2 30 2560
Fragments arranged by size:
2560 1695 32 30 30 2
HincII GTy'rAC
Cuts at: 687 1388 687
Size: 701 3648
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 452 1369 452
Size: 917 3432
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 3020 3204 188
Size: 2832 184 1333
Fragments arranged by size:
2832 1333 184
MscI TGG'CCA
Cuts at: 985 1000 2110 985
Size: 15 1110 3224
Fragments arranged by size:
3224 1110 15
MslI CAynn'nnrTG
Cuts at: 572 745 2338 2465 572
Size: 173 1593 127 2456
Fragments arranged by size:
2456 1593 173 127
MspA1I CmG'CkG
Cuts at: 153 1134 1460 1774 2134 3147 3392 153
Size: 981 326 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 981 360 326 314 245
NaeI GCC'GGC
Cuts at: 1234 2530 1234
Size: 1296 3053
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 107 782 107
Size: 675 3674
NgoMIV G'CCGG_C
Cuts at: 1232 2528 1232
Size: 1296 3053
NspI r_CATG'y
Cuts at: 1402 1902 2809 1402
Size: 500 907 2942
Fragments arranged by size:
2942 907 500
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PflMI CCAn_nnn'nTGG
Cuts at: 992 1050 992
Size: 58 4291
PfoI T'CCnGG_A
Cuts at: 353 353
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 888 1292 888
Size: 404 3945
PspXI vC'TCGA_Gb
Cuts at: 589 589
Size: 4349
PstI C_TGCA'G
Cuts at: 610 821 842 1133 1241 1283 1396 2081 610
Size: 211 21 291 108 42 113 685 2878
Fragments arranged by size:
2878 685 291 211 113 108 42 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 153 1774 2134 153
Size: 1621 360 2368
Fragments arranged by size:
2368 1621 360
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 217 1190 1363 217
Size: 973 173 3203
Fragments arranged by size:
3203 973 173
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SanDI GG'GwC_CC
Cuts at: 1292 1292
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 205 2372 2582 205
Size: 2167 210 1972
Fragments arranged by size:
2167 1972 210
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 606 817 838 1129 1237 1279 1392 2077
Size: 573 211 21 291 108 42 113 685
Cuts at: 2077 3070 3261 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 573 291 211 191 113
108 42 21
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 328 1371 328
Size: 1043 3306
SmlI C'TyrA_G
Cuts at: 589 1084 1504 2419 2911 3173 589
Size: 495 420 915 492 262 1765
Fragments arranged by size:
1765 915 495 492 420 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StyI C'CwwG_G
Cuts at: 107 435 782 1483 1837 107
Size: 328 347 701 354 2619
Fragments arranged by size:
2619 701 354 347 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 952 967 984 1932 2065 2524 2733 952
Size: 15 17 948 133 459 209 2568
Fragments arranged by size:
2568 948 459 209 133 17 15
TatI w'GTAC_w
Cuts at: 3864 3864
Size: 4349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 880 993 1008 1844 880
Size: 113 15 836 3385
Fragments arranged by size:
3385 836 113 15
XhoI C'TCGA_G
Cuts at: 589 589
Size: 4349
XmaI C'CCGG_G
Cuts at: 326 1369 326
Size: 1043 3306
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI