Restriction map for
pF1KB6616
(Circular) (Six-base) MAPSORT of: pF1KB6616.seq Check: 5867 from: 1 to: 4412
pF1KB6616 4412 bp
With 182 enzymes: SgfI *
June 30, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 742 742
Size: 4412
AccI GT'mk_AC
Cuts at: 1450 1450
Size: 4412
Acc65I G'GTAC_C
Cuts at: 1428 1428
Size: 4412
AclI AA'CG_TT
Cuts at: 4252 4252
Size: 4412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1076 2237 2669 3416 3684 1076
Size: 1161 432 747 268 1804
Fragments arranged by size:
1804 1161 747 432 268
AflIII A'CryG_T
Cuts at: 176 1764 2868 3845 176
Size: 1588 1104 977 743
Fragments arranged by size:
1588 1104 977 743
AgeI A'CCGG_T
Cuts at: 664 1097 2800 664
Size: 433 1703 2276
Fragments arranged by size:
2276 1703 433
AhdI GACnn_n'nnGTC
Cuts at: 822 822
Size: 4412
AlwNI CAG_nnn'CTG
Cuts at: 132 1717 3284 132
Size: 1585 1567 1260
Fragments arranged by size:
1585 1567 1260
ApaI G_GGCC'C
Cuts at: 1338 1338
Size: 4412
ApaLI G'TGCA_C
Cuts at: 625 3182 625
Size: 2557 1855
ApoI r'AATT_y
Cuts at: 401 1416 3765 4021 401
Size: 1015 2349 256 792
Fragments arranged by size:
2349 1015 792 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
AvaI C'yCGr_G
Cuts at: 772 930 1432 772
Size: 158 502 3752
Fragments arranged by size:
3752 502 158
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 564 597 564
Size: 33 4379
BamHI G'GATC_C
Cuts at: 368 1437 368
Size: 1069 3343
BanI G'GyrC_C
Cuts at: 670 1119 1209 1428 1842 2090 2125 670
Size: 449 90 219 414 248 35 2957
Fragments arranged by size:
2957 449 414 248 219 90 35
BanII G_rGCy'C
Cuts at: 754 1084 1338 1426 2456 754
Size: 330 254 88 1030 2710
Fragments arranged by size:
2710 1030 330 254 88
BbeI G_GCGC'C
Cuts at: 1846 2094 1846
Size: 248 4164
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3071 4412 2310
Size: 761 1341 2310
Fragments arranged by size:
2310 1341 761
BclI T'GATC_A
Cuts at: 1932 1932
Size: 4412
BglI GCCn_nnn'nGGC
Cuts at: 1354 1354
Size: 4412
BglII A'GATC_T
Cuts at: 1927 3964 1927
Size: 2037 2375
BlpI GC'TnA_GC
Cuts at: 1306 1524 1306
Size: 218 4194
Bme1580I G_kGCm'C
Cuts at: 629 1070 1214 1338 2037 2130 3186 629
Size: 441 144 124 699 93 1056 1855
Fragments arranged by size:
1855 1056 699 441 144 124 93
BmrI ACTGGGnnnn_n'
Cuts at: 547 1624 1705 2040 4184 547
Size: 1077 81 335 2144 775
Fragments arranged by size:
2144 1077 775 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 4412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 619
Size: 4412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 997 1588 2467 2959 3257 331
Size: 666 591 879 492 298 1486
Fragments arranged by size:
1486 879 666 591 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4116 4116
Size: 4412
BsaAI yAC'GTr
Cuts at: 1765 1765
Size: 4412
BsaBI GATnn'nnATC
Cuts at: 6 1926 3963 6
Size: 1920 2037 455
Fragments arranged by size:
2037 1920 455
BsaHI Gr'CG_yC
Cuts at: 1843 2091 2793 4289 1843
Size: 248 702 1496 1966
Fragments arranged by size:
1966 1496 702 248
BsaWI w'CCGG_w
Cuts at: 664 1034 1097 1601 2122 2800 3074 3221 3632
Size: 370 63 504 521 678 274 147 411
Cuts at: 3632 664
Size: 1444
Fragments arranged by size:
1444 678 521 504 411 370 274 147
63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 307 337 784 814 307
Size: 30 447 30 3905
Fragments arranged by size:
3905 447 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 429 1246 429
Size: 817 3595
BseYI C'CCAG_C
Cuts at: 132 270 829 1837 2595 3172 3668 132
Size: 138 559 1008 758 577 496 876
Fragments arranged by size:
1008 876 758 577 559 496 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 694 1123 345
Size: 349 429 3634
Fragments arranged by size:
3634 429 349
BsiEI CG_ry'CG
Cuts at: 104 1954 2000 3208 104
Size: 1850 46 1208 1308
Fragments arranged by size:
1850 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 629 1312 1426 2204 2394 3186 629
Size: 683 114 778 190 792 1855
Fragments arranged by size:
1855 792 778 683 190 114
BsmBI CGTCTCn'nnnn_
Cuts at: 310 310
Size: 4412
Bsp1286I G_dGCh'C
Cuts at: 629 754 1070 1084 1214 1312 1338 1426 2037
Size: 125 316 14 130 98 26 88 611
Cuts at: 2037 2130 2204 2394 2456 3186 629
Size: 93 74 190 62 730 1855
Fragments arranged by size:
1855 730 611 316 190 130 125 98
93 88 74 62 26 14
BspEI T'CCGG_A
Cuts at: 1034 3632 1034
Size: 2598 1814
BspHI T'CATG_A
Cuts at: 3588 3588
Size: 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 614 659 742 968 1145 1395 1462 1676
Size: 339 45 83 226 177 250 67 214
Cuts at: 1676 1978 2359 275
Size: 302 381 2328
Fragments arranged by size:
2328 381 339 302 250 226 214 177
83 67 45
BsrBI CCG'CTC
Cuts at: 50 951 2704 2758 4242 4407 50
Size: 901 1753 54 1484 165 55
Fragments arranged by size:
1753 1484 901 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2324 2324
Size: 4412
BsrFI r'CCGG_y
Cuts at: 664 987 1097 1382 2410 2591 2800 664
Size: 323 110 285 1028 181 209 2276
Fragments arranged by size:
2276 1028 323 285 209 181 110
BssSI C'ACGA_G
Cuts at: 2683 3041 2683
Size: 358 4054
BstAPI GCAn_nnn'nTGC
Cuts at: 522 1358 1789 522
Size: 836 431 3145
Fragments arranged by size:
3145 836 431
BstBI TT'CG_AA
Cuts at: 872 2773 872
Size: 1901 2511
BstEII G'GTnAC_C
Cuts at: 834 834
Size: 4412
BstXI CCAn_nnnn'nTGG
Cuts at: 114 789 114
Size: 675 3737
BstYI r'GATC_y
Cuts at: 1 341 368 1013 1376 1437 1904 1927 2262
Size: 340 27 645 363 61 467 23 335
Cuts at: 2262 2508 3520 3606 3618 3964 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 645 467 449 363 346 340 335
246 86 61 27 23 12
BtgI C'CryG_G
Cuts at: 107 567 854 107
Size: 460 287 3665
Fragments arranged by size:
3665 460 287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2362 2543 2706 2362
Size: 181 163 4068
Fragments arranged by size:
4068 181 163
BtsI GCAGTG_nn'
Cuts at: 706 1655 706
Size: 949 3463
DraI TTT'AAA
Cuts at: 1411 1411
Size: 4412
DrdI GACnn_nn'nnGTC
Cuts at: 2118 2976 2118
Size: 858 3554
EaeI y'GGCC_r
Cuts at: 698 989 1997 2171 2562 2589 3934 698
Size: 291 1008 174 391 27 1345 1176
Fragments arranged by size:
1345 1176 1008 391 291 174 27
EagI C'GGCC_G
Cuts at: 1997 1997
Size: 4412
EarI CTCTTCn'nnn_
Cuts at: 461 1171 1223 2435 2645 461
Size: 710 52 1212 210 2228
Fragments arranged by size:
2228 1212 710 210 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 739 1339 1353 1369 2620 2930 3076 4222 739
Size: 600 14 16 1251 310 146 1146 929
Fragments arranged by size:
1251 1146 929 600 310 146 16 14
EcoICRI GAG'CTC
Cuts at: 1424 1424
Size: 4412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1076 2237 2669 3416 3684 619
Size: 457 1161 432 747 268 1347
Fragments arranged by size:
1347 1161 747 457 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1267 1267
Size: 4412
EcoO109I rG'GnC_Cy
Cuts at: 264 1134 1161 1221 1260 1335 1551 264
Size: 870 27 60 39 75 216 3125
Fragments arranged by size:
3125 870 216 75 60 39 27
EcoRI G'AATT_C
Cuts at: 1416 1416
Size: 4412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 991 2431 991
Size: 1440 2972
FspI TGC'GCA
Cuts at: 2193 2193
Size: 4412
HaeII r_GCGC'y
Cuts at: 1257 1846 2094 3116 4095 1257
Size: 589 248 1022 979 1574
Fragments arranged by size:
1574 1022 979 589 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 784 808 816 840 2254 2284 2286
Size: 32 607 24 8 24 1414 30 2
Cuts at: 2286 2316 145
Size: 30 2241
Fragments arranged by size:
2241 1414 607 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 1451 1451
Size: 4412
KasI G'GCGC_C
Cuts at: 1842 2090 1842
Size: 248 4164
KpnI G_GTAC'C
Cuts at: 1432 1432
Size: 4412
MluI A'CGCG_T
Cuts at: 3845 3845
Size: 4412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 710 3083 3267 710
Size: 2373 184 1855
Fragments arranged by size:
2373 1855 184
MscI TGG'CCA
Cuts at: 2173 2173
Size: 4412
MslI CAynn'nnrTG
Cuts at: 112 431 1114 2401 2528 112
Size: 319 683 1287 127 1996
Fragments arranged by size:
1996 1287 683 319 127
MspA1I CmG'CkG
Cuts at: 132 444 585 1028 1305 1523 1837 2197 3210
Size: 312 141 443 277 218 314 360 1013
Cuts at: 3210 3455 132
Size: 245 1089
Fragments arranged by size:
1089 1013 443 360 314 312 277 245
218 141
NaeI GCC'GGC
Cuts at: 1384 2593 1384
Size: 1209 3203
NarI GG'CG_CC
Cuts at: 1843 2091 1843
Size: 248 4164
NcoI C'CATG_G
Cuts at: 107 107
Size: 4412
NgoMIV G'CCGG_C
Cuts at: 1382 2591 1382
Size: 1209 3203
NspI r_CATG'y
Cuts at: 141 516 1465 1965 2872 141
Size: 375 949 500 907 1681
Fragments arranged by size:
1681 949 907 500 375
PciI A'CATG_T
Cuts at: 2868 2868
Size: 4412
PflMI CCAn_nnn'nTGG
Cuts at: 447 447
Size: 4412
PmeI GTTT'AAAC
Cuts at: 1411 1411
Size: 4412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4380
PpuMI rG'GwC_Cy
Cuts at: 264 1134 1161 1221 264
Size: 870 27 60 3455
Fragments arranged by size:
3455 870 60 27
PspOMI G'GGCC_C
Cuts at: 1334 1334
Size: 4412
PstI C_TGCA'G
Cuts at: 1459 2144 1459
Size: 685 3727
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4412
PvuII CAG'CTG
Cuts at: 132 444 585 1028 1305 1837 2197 132
Size: 312 141 443 277 532 360 2347
Fragments arranged by size:
2347 532 443 360 312 277 141
RsrII CG'GwC_CG
Cuts at: 2607 2607
Size: 4412
SacI G_AGCT'C
Cuts at: 1426 1426
Size: 4412
SalI G'TCGA_C
Cuts at: 1449 1449
Size: 4412
SapI GCTCTTCn'nnn_
Cuts at: 2435 2645 2435
Size: 210 4202
SbfI CC_TGCA'GG
Cuts at: 1459 1459
Size: 4412
ScaI AGT'ACT
Cuts at: 499 3929 499
Size: 3430 982
SfcI C'TryA_G
Cuts at: 33 1455 2140 3133 3324 33
Size: 1422 685 993 191 1121
Fragments arranged by size:
1422 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1844 2092 1844
Size: 248 4164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
SgrAI Cr'CCGG_yG
Cuts at: 664 664
Size: 4412
SmaI CCC'GGG
Cuts at: 932 1434 932
Size: 502 3910
SmlI C'TyrA_G
Cuts at: 346 976 1567 2482 2974 3236 346
Size: 630 591 915 492 262 1522
Fragments arranged by size:
1522 915 630 591 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4412
SphI G_CATG'C
Cuts at: 516 1465 1965 516
Size: 949 500 2963
Fragments arranged by size:
2963 949 500
StuI AGG'CCT
Cuts at: 1357 1357
Size: 4412
StyI C'CwwG_G
Cuts at: 107 1091 1268 1546 1900 107
Size: 984 177 278 354 2619
Fragments arranged by size:
2619 984 354 278 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1995 2128 2587 2796 1995
Size: 133 459 209 3611
Fragments arranged by size:
3611 459 209 133
TatI w'GTAC_w
Cuts at: 497 3927 497
Size: 3430 982
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 307 339 3929 3961 307
Size: 32 3590 32 758
Fragments arranged by size:
3590 758 32 32
Tth111I GACn'n_nGTC
Cuts at: 2209 2209
Size: 4412
XbaI T'CTAG_A
Cuts at: 1443 1443
Size: 4412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1907 114
Size: 1793 2619
XmaI C'CCGG_G
Cuts at: 930 1432 930
Size: 502 3910
XmnI GAAnn'nnTTC
Cuts at: 547 547
Size: 4412
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI ZraI