Restriction map for
pF1KB6606
(Circular) (Six-base) MAPSORT of: pF1KB6606.seq Check: 6278 from: 1 to: 4406
pF1KB6606 4406 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 175 175
Size: 4406
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 2231 2663 3410 3678 727
Size: 1504 432 747 268 1455
Fragments arranged by size:
1504 1455 747 432 268
AflIII A'CryG_T
Cuts at: 1758 2862 3839 1758
Size: 1104 977 2325
Fragments arranged by size:
2325 1104 977
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 410 410
Size: 4406
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 631 663 631
Size: 32 4374
AlwNI CAG_nnn'CTG
Cuts at: 267 1236 1711 3278 267
Size: 969 475 1567 1395
Fragments arranged by size:
1567 1395 969 475
ApaI G_GGCC'C
Cuts at: 976 976
Size: 4406
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 391 1410 3759 4015 391
Size: 1019 2349 256 782
Fragments arranged by size:
2349 1019 782 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 137 221 755 1298 1345 1426 137
Size: 84 534 543 47 81 3117
Fragments arranged by size:
3117 543 534 84 81 47
BamHI G'GATC_C
Cuts at: 1226 1431 1226
Size: 205 4201
BanI G'GyrC_C
Cuts at: 163 315 342 1422 1836 2084 2119 163
Size: 152 27 1080 414 248 35 2450
Fragments arranged by size:
2450 1080 414 248 152 35 27
BanII G_rGCy'C
Cuts at: 976 1307 1420 2450 976
Size: 331 113 1030 2932
Fragments arranged by size:
2932 1030 331 113
BbeI G_GCGC'C
Cuts at: 346 1840 2088 346
Size: 1494 248 2664
Fragments arranged by size:
2664 1494 248
BbsI GAAGACnn'nnnn_
Cuts at: 519 1167 1269 1370 519
Size: 648 102 101 3555
Fragments arranged by size:
3555 648 102 101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 357 391 357
Size: 34 4372
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 1926 1926
Size: 4406
BglI GCCn_nnn'nGGC
Cuts at: 934 934
Size: 4406
BglII A'GATC_T
Cuts at: 662 1921 3958 662
Size: 1259 2037 1110
Fragments arranged by size:
2037 1259 1110
BlpI GC'TnA_GC
Cuts at: 1518 1518
Size: 4406
Bme1580I G_kGCm'C
Cuts at: 320 755 976 2031 2124 3180 320
Size: 435 221 1055 93 1056 1546
Fragments arranged by size:
1546 1056 1055 435 221 93
BmrI ACTGGGnnnn_n'
Cuts at: 742 1618 1699 2034 4178 742
Size: 876 81 335 2144 970
Fragments arranged by size:
2144 970 876 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1129 1291 1348 622
Size: 507 162 57 3680
Fragments arranged by size:
3680 507 162 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1582 2461 2953 3251 676
Size: 906 879 492 298 1831
Fragments arranged by size:
1831 906 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1237 4110 1237
Size: 2873 1533
BsaAI yAC'GTr
Cuts at: 1759 1759
Size: 4406
BsaBI GATnn'nnATC
Cuts at: 6 661 1920 3957 6
Size: 655 1259 2037 455
Fragments arranged by size:
2037 1259 655 455
BsaHI Gr'CG_yC
Cuts at: 343 1066 1837 2085 2787 4283 343
Size: 723 771 248 702 1496 466
Fragments arranged by size:
1496 771 723 702 466 248
BsaWI w'CCGG_w
Cuts at: 282 1595 2116 2794 3068 3215 3626 282
Size: 1313 521 678 274 147 411 1062
Fragments arranged by size:
1313 1062 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 631 661 631
Size: 30 4376
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1060 1087 1134 1345 1060
Size: 27 47 211 4121
Fragments arranged by size:
4121 211 47 27
BseYI C'CCAG_C
Cuts at: 113 274 887 1127 1831 2589 3166 3662 113
Size: 161 613 240 704 758 577 496 857
Fragments arranged by size:
857 758 704 613 577 496 240 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 893 406
Size: 487 3919
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 404 1200 1307 1420 2198 2388 3180 404
Size: 796 107 113 778 190 792 1630
Fragments arranged by size:
1630 796 792 778 190 113 107
BsmBI CGTCTCn'nnnn_
Cuts at: 634 1057 634
Size: 423 3983
Bsp1286I G_dGCh'C
Cuts at: 320 404 755 976 1200 1307 1420 2031 2124
Size: 84 351 221 224 107 113 611 93
Cuts at: 2124 2198 2388 2450 3180 320
Size: 74 190 62 730 1546
Fragments arranged by size:
1546 730 611 351 224 221 190 113
107 93 84 74 62
BspEI T'CCGG_A
Cuts at: 3626 3626
Size: 4406
BspHI T'CATG_A
Cuts at: 728 1200 3582 728
Size: 472 2382 1552
Fragments arranged by size:
2382 1552 472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 175 770 1382 1456 1670 1972 2353 175
Size: 595 612 74 214 302 381 2228
Fragments arranged by size:
2228 612 595 381 302 214 74
BsrBI CCG'CTC
Cuts at: 50 477 2698 2752 4236 4401 50
Size: 427 2221 54 1484 165 55
Fragments arranged by size:
2221 1484 427 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2318 2318
Size: 4406
BsrFI r'CCGG_y
Cuts at: 212 2404 2585 2794 212
Size: 2192 181 209 1824
Fragments arranged by size:
2192 1824 209 181
BsrGI T'GTAC_A
Cuts at: 491 491
Size: 4406
BssSI C'ACGA_G
Cuts at: 2677 3035 2677
Size: 358 4048
BstAPI GCAn_nnn'nTGC
Cuts at: 384 408 1783 384
Size: 24 1375 3007
Fragments arranged by size:
3007 1375 24
BstBI TT'CG_AA
Cuts at: 2767 2767
Size: 4406
BstEII G'GTnAC_C
Cuts at: 831 831
Size: 4406
BstXI CCAn_nnnn'nTGG
Cuts at: 132 312 1188 132
Size: 180 876 3350
Fragments arranged by size:
3350 876 180
BstYI r'GATC_y
Cuts at: 1 662 879 1226 1265 1431 1898 1921 2256
Size: 661 217 347 39 166 467 23 335
Cuts at: 2256 2502 3514 3600 3612 3958 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 661 467 449 347 346 335 246
217 166 86 39 23 12
BtgI C'CryG_G
Cuts at: 618 1271 618
Size: 653 3753
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2356 2537 2700 2356
Size: 181 163 4062
Fragments arranged by size:
4062 181 163
BtsI GCAGTG_nn'
Cuts at: 231 1649 231
Size: 1418 2988
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DraIII CAC_nnn'GTG
Cuts at: 408 624 408
Size: 216 4190
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 785 1991 2165 2556 2583 3928 785
Size: 1206 174 391 27 1345 1263
Fragments arranged by size:
1345 1263 1206 391 174 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 1055 2429 2639 1055
Size: 1374 210 2822
Fragments arranged by size:
2822 1374 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1270 2614 2924 3070 4216 1270
Size: 1344 310 146 1146 1460
Fragments arranged by size:
1460 1344 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1305 1418 1305
Size: 113 4293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 727 1129 1291 1348 2231 2663 3410 3678
Size: 105 402 162 57 883 432 747 268
Cuts at: 3678 622
Size: 1350
Fragments arranged by size:
1350 883 747 432 402 268 162 105
57
EcoNI CCTnn'n_nnAGG
Cuts at: 766 1345 766
Size: 579 3827
EcoO109I rG'GnC_Cy
Cuts at: 152 204 225 327 480 759 771 972 973
Size: 52 21 102 153 279 12 201 1
Cuts at: 973 1168 1233 1545 152
Size: 195 65 312 3013
Fragments arranged by size:
3013 312 279 201 195 153 102 65
52 21 12 1
EcoRI G'AATT_C
Cuts at: 1410 1410
Size: 4406
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 387 543 2425 387
Size: 156 1882 2368
Fragments arranged by size:
2368 1882 156
FspI TGC'GCA
Cuts at: 451 2187 451
Size: 1736 2670
HaeII r_GCGC'y
Cuts at: 346 1840 2088 3110 4089 346
Size: 1494 248 1022 979 663
Fragments arranged by size:
1494 1022 979 663 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 903 935 2248 2278 2280 2310 903
Size: 32 1313 30 2 30 2999
Fragments arranged by size:
2999 1313 32 30 30 2
HincII GTy'rAC
Cuts at: 738 1445 738
Size: 707 3699
KasI G'GCGC_C
Cuts at: 342 1836 2084 342
Size: 1494 248 2664
Fragments arranged by size:
2664 1494 248
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3077 3261 3077
Size: 184 4222
MscI TGG'CCA
Cuts at: 787 2167 787
Size: 1380 3026
MslI CAynn'nnrTG
Cuts at: 1186 2395 2522 1186
Size: 1209 127 3070
Fragments arranged by size:
3070 1209 127
MspA1I CmG'CkG
Cuts at: 244 501 1101 1517 1831 2191 3204 3449 244
Size: 257 600 416 314 360 1013 245 1201
Fragments arranged by size:
1201 1013 600 416 360 314 257 245
NaeI GCC'GGC
Cuts at: 214 2587 214
Size: 2373 2033
NarI GG'CG_CC
Cuts at: 343 1837 2085 343
Size: 1494 248 2664
Fragments arranged by size:
2664 1494 248
NgoMIV G'CCGG_C
Cuts at: 212 2585 212
Size: 2373 2033
NspI r_CATG'y
Cuts at: 1459 1959 2866 1459
Size: 500 907 2999
Fragments arranged by size:
2999 907 500
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4406
PfoI T'CCnGG_A
Cuts at: 599 599
Size: 4406
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 631 663 3545 3577 631
Size: 32 2882 32 1460
Fragments arranged by size:
2882 1460 32 32
PpuMI rG'GwC_Cy
Cuts at: 204 327 480 759 771 204
Size: 123 153 279 12 3839
Fragments arranged by size:
3839 279 153 123 12
PspOMI G'GGCC_C
Cuts at: 972 972
Size: 4406
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1072 1104 1072
Size: 32 4374
PstI C_TGCA'G
Cuts at: 238 380 1241 1323 1379 1453 2138 238
Size: 142 861 82 56 74 685 2506
Fragments arranged by size:
2506 861 685 142 82 74 56
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 244 501 1101 1831 2191 244
Size: 257 600 730 360 2459
Fragments arranged by size:
2459 730 600 360 257
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1307 1420 1307
Size: 113 4293
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 1241 1453 1241
Size: 212 4194
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SexAI A'CCwGG_T
Cuts at: 842 842
Size: 4406
SfcI C'TryA_G
Cuts at: 33 147 234 376 1237 1319 1375 1449 2134
Size: 114 87 142 861 82 56 74 685
Cuts at: 2134 3127 3318 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 861 685 191 142 114 87
82 74 56
SfiI GGCCn_nnn'nGGCC
Cuts at: 934 934
Size: 4406
SfoI GGC'GCC
Cuts at: 344 1838 2086 344
Size: 1494 248 2664
Fragments arranged by size:
2664 1494 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 757 1300 1428 757
Size: 543 128 3735
Fragments arranged by size:
3735 543 128
SmlI C'TyrA_G
Cuts at: 691 1561 2476 2968 3230 691
Size: 870 915 492 262 1867
Fragments arranged by size:
1867 915 870 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StuI AGG'CCT
Cuts at: 143 143
Size: 4406
StyI C'CwwG_G
Cuts at: 305 518 1540 1894 305
Size: 213 1022 354 2817
Fragments arranged by size:
2817 1022 354 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1989 2122 2581 2790 1989
Size: 133 459 209 3605
Fragments arranged by size:
3605 459 209 133
TatI w'GTAC_w
Cuts at: 491 3921 491
Size: 3430 976
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4374
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 309 693 1254 1901 309
Size: 384 561 647 2814
Fragments arranged by size:
2814 647 561 384
XmaI C'CCGG_G
Cuts at: 755 1298 1426 755
Size: 543 128 3735
Fragments arranged by size:
3735 543 128
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BssHII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI SacII SanDI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI