Restriction map for
pF1KB6604
(Circular) (Six-base) MAPSORT of: pF1KB6604.seq Check: 9409 from: 1 to: 4406
pF1KB6604 4406 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1444 1444
Size: 4406
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 423 1181 1378 2231 2663 3410 3678 183
Size: 240 758 197 853 432 747 268 911
Fragments arranged by size:
911 853 758 747 432 268 240 197
AflIII A'CryG_T
Cuts at: 1758 2862 3839 1758
Size: 1104 977 2325
Fragments arranged by size:
2325 1104 977
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1328 1328
Size: 4406
AlwNI CAG_nnn'CTG
Cuts at: 1251 1711 3278 1251
Size: 460 1567 2379
Fragments arranged by size:
2379 1567 460
ApaI G_GGCC'C
Cuts at: 401 454 917 401
Size: 53 463 3890
Fragments arranged by size:
3890 463 53
ApaLI G'TGCA_C
Cuts at: 217 3176 217
Size: 2959 1447
ApoI r'AATT_y
Cuts at: 541 625 1410 3759 4015 541
Size: 84 785 2349 256 932
Fragments arranged by size:
2349 932 785 256 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 222 1426 222
Size: 1204 3202
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 732 765 732
Size: 33 4373
BamHI G'GATC_C
Cuts at: 1431 1431
Size: 4406
BanI G'GyrC_C
Cuts at: 135 162 239 342 1278 1422 1836 2084 2119
Size: 27 77 103 936 144 414 248 35
Cuts at: 2119 135
Size: 2422
Fragments arranged by size:
2422 936 414 248 144 103 77 35
27
BanII G_rGCy'C
Cuts at: 401 454 917 1420 2450 401
Size: 53 463 503 1030 2357
Fragments arranged by size:
2357 1030 503 463 53
BbeI G_GCGC'C
Cuts at: 139 243 1840 2088 139
Size: 104 1597 248 2457
Fragments arranged by size:
2457 1597 248 104
BbsI GAAGACnn'nnnn_
Cuts at: 429 429
Size: 4406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4372
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 1926 1926
Size: 4406
BglI GCCn_nnn'nGGC
Cuts at: 1060 1060
Size: 4406
BglII A'GATC_T
Cuts at: 1921 3958 1921
Size: 2037 2369
BlpI GC'TnA_GC
Cuts at: 1518 1518
Size: 4406
Bme1580I G_kGCm'C
Cuts at: 165 221 401 454 917 1283 2031 2124 3180
Size: 56 180 53 463 366 748 93 1056
Cuts at: 3180 165
Size: 1391
Fragments arranged by size:
1391 1056 748 463 366 180 93 56
53
BmrI ACTGGGnnnn_n'
Cuts at: 819 1618 1699 2034 4178 819
Size: 799 81 335 2144 1047
Fragments arranged by size:
2144 1047 799 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 237 300 2773 237
Size: 63 2473 1870
Fragments arranged by size:
2473 1870 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 685 307
Size: 378 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1582 2461 2953 3251 1582
Size: 879 492 298 2737
Fragments arranged by size:
2737 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1121 4110 1121
Size: 2989 1417
BsaAI yAC'GTr
Cuts at: 114 1759 114
Size: 1645 2761
BsaBI GATnn'nnATC
Cuts at: 6 1920 3957 6
Size: 1914 2037 455
Fragments arranged by size:
2037 1914 455
BsaHI Gr'CG_yC
Cuts at: 136 240 494 1024 1837 2085 2787 4283 136
Size: 104 254 530 813 248 702 1496 259
Fragments arranged by size:
1496 813 702 530 259 254 248 104
BsaWI w'CCGG_w
Cuts at: 575 629 677 980 1595 2116 2794 3068 3215
Size: 54 48 303 615 521 678 274 147
Cuts at: 3215 3626 575
Size: 411 1355
Fragments arranged by size:
1355 678 615 521 411 303 274 147
54 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 781 400
Size: 381 4025
BseYI C'CCAG_C
Cuts at: 1831 2589 3166 3662 1831
Size: 758 577 496 2575
Fragments arranged by size:
2575 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 1264
Size: 4406
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 221 701 710 1420 2198 2388 3180 221
Size: 480 9 710 778 190 792 1447
Fragments arranged by size:
1447 792 778 710 480 190 9
BsmBI CGTCTCn'nnnn_
Cuts at: 664 850 664
Size: 186 4220
Bsp1286I G_dGCh'C
Cuts at: 165 221 401 454 701 710 917 1283 1420
Size: 56 180 53 247 9 207 366 137
Cuts at: 1420 2031 2124 2198 2388 2450 3180 165
Size: 611 93 74 190 62 730 1391
Fragments arranged by size:
1391 730 611 366 247 207 190 180
137 93 74 62 56 53 9
BspEI T'CCGG_A
Cuts at: 629 677 3626 629
Size: 48 2949 1409
Fragments arranged by size:
2949 1409 48
BspHI T'CATG_A
Cuts at: 506 3582 506
Size: 3076 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1456 1670 1972 2353 1456
Size: 214 302 381 3509
Fragments arranged by size:
3509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 258 1016 2698 2752 4236 4401 50
Size: 208 758 1682 54 1484 165 55
Fragments arranged by size:
1682 1484 758 208 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2318 2318
Size: 4406
BsrFI r'CCGG_y
Cuts at: 236 2404 2585 2794 236
Size: 2168 181 209 1848
Fragments arranged by size:
2168 1848 209 181
BsrGI T'GTAC_A
Cuts at: 365 365
Size: 4406
BssSI C'ACGA_G
Cuts at: 523 1105 2677 3035 523
Size: 582 1572 358 1894
Fragments arranged by size:
1894 1572 582 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1783 1783
Size: 4406
BstBI TT'CG_AA
Cuts at: 2767 2767
Size: 4406
BstXI CCAn_nnnn'nTGG
Cuts at: 381 381
Size: 4406
BstYI r'GATC_y
Cuts at: 1 278 593 632 726 1431 1898 1921 2256
Size: 277 315 39 94 705 467 23 335
Cuts at: 2256 2502 3514 3600 3612 3958 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 705 467 449 346 335 315 277
246 94 86 39 23 12
BtgI C'CryG_G
Cuts at: 374 823 956 986 374
Size: 449 133 30 3794
Fragments arranged by size:
3794 449 133 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1137 2356 2537 2700 1137
Size: 1219 181 163 2843
Fragments arranged by size:
2843 1219 181 163
BtsI GCAGTG_nn'
Cuts at: 121 1649 121
Size: 1528 2878
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DraIII CAC_nnn'GTG
Cuts at: 813 813
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 191 405 461 1052 1991 2165 2556 2583 3928
Size: 214 56 591 939 174 391 27 1345
Cuts at: 3928 191
Size: 669
Fragments arranged by size:
1345 939 669 591 391 214 174 56
27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 736 2429 2639 736
Size: 1693 210 2503
Fragments arranged by size:
2503 1693 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 174 2614 2924 3070 4216 174
Size: 2440 310 146 1146 364
Fragments arranged by size:
2440 1146 364 310 146
EcoICRI GAG'CTC
Cuts at: 1418 1418
Size: 4406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 307 423 685 1181 1378 2231 2663 3410
Size: 124 116 262 496 197 853 432 747
Cuts at: 3410 3678 183
Size: 268 911
Fragments arranged by size:
911 853 747 496 432 268 262 197
124 116
EcoO109I rG'GnC_Cy
Cuts at: 450 451 914 1545 450
Size: 1 463 631 3311
Fragments arranged by size:
3311 631 463 1
EcoRI G'AATT_C
Cuts at: 541 625 1410 541
Size: 84 785 3537
Fragments arranged by size:
3537 785 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 453 561 948 2425 453
Size: 108 387 1477 2434
Fragments arranged by size:
2434 1477 387 108
FspI TGC'GCA
Cuts at: 2187 2187
Size: 4406
HaeII r_GCGC'y
Cuts at: 139 157 243 1840 2088 3110 4089 139
Size: 18 86 1597 248 1022 979 456
Fragments arranged by size:
1597 1022 979 456 248 86 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1327 1359 2248 2278 2280 2310 1327
Size: 32 889 30 2 30 3423
Fragments arranged by size:
3423 889 32 30 30 2
HincII GTy'rAC
Cuts at: 519 1445 519
Size: 926 3480
KasI G'GCGC_C
Cuts at: 135 239 1836 2084 135
Size: 104 1597 248 2457
Fragments arranged by size:
2457 1597 248 104
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1370 3077 3261 1370
Size: 1707 184 2515
Fragments arranged by size:
2515 1707 184
MscI TGG'CCA
Cuts at: 463 1054 2167 463
Size: 591 1113 2702
Fragments arranged by size:
2702 1113 591
MslI CAynn'nnrTG
Cuts at: 112 352 379 505 2395 2522 112
Size: 240 27 126 1890 127 1996
Fragments arranged by size:
1996 1890 240 127 126 27
MspA1I CmG'CkG
Cuts at: 825 1517 1831 2191 3204 3449 825
Size: 692 314 360 1013 245 1782
Fragments arranged by size:
1782 1013 692 360 314 245
NaeI GCC'GGC
Cuts at: 2587 2587
Size: 4406
NarI GG'CG_CC
Cuts at: 136 240 1837 2085 136
Size: 104 1597 248 2457
Fragments arranged by size:
2457 1597 248 104
NcoI C'CATG_G
Cuts at: 374 374
Size: 4406
NgoMIV G'CCGG_C
Cuts at: 2585 2585
Size: 4406
NspI r_CATG'y
Cuts at: 1459 1959 2866 1459
Size: 500 907 2999
Fragments arranged by size:
2999 907 500
PasI CC'CwG_GG
Cuts at: 447 447
Size: 4406
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PflMI CCAn_nnn'nTGG
Cuts at: 813 813
Size: 4406
PfoI T'CCnGG_A
Cuts at: 650 701 650
Size: 51 4355
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PmlI CAC'GTG
Cuts at: 114 114
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 4374
PshAI GACnn'nnGTC
Cuts at: 1252 1252
Size: 4406
PspOMI G'GGCC_C
Cuts at: 397 450 913 397
Size: 53 463 3890
Fragments arranged by size:
3890 463 53
PspXI vC'TCGA_Gb
Cuts at: 222 222
Size: 4406
PstI C_TGCA'G
Cuts at: 845 1453 2138 845
Size: 608 685 3113
Fragments arranged by size:
3113 685 608
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 1831 2191 1831
Size: 360 4046
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1420 1420
Size: 4406
SacII CC_GC'GG
Cuts at: 826 826
Size: 4406
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SfcI C'TryA_G
Cuts at: 33 841 1449 2134 3127 3318 33
Size: 808 608 685 993 191 1121
Fragments arranged by size:
1121 993 808 685 608 191
SfoI GGC'GCC
Cuts at: 137 241 1838 2086 137
Size: 104 1597 248 2457
Fragments arranged by size:
2457 1597 248 104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SgrAI Cr'CCGG_yG
Cuts at: 236 236
Size: 4406
SmaI CCC'GGG
Cuts at: 1428 1428
Size: 4406
SmlI C'TyrA_G
Cuts at: 222 1561 2476 2968 3230 222
Size: 1339 915 492 262 1398
Fragments arranged by size:
1398 1339 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StuI AGG'CCT
Cuts at: 1093 1093
Size: 4406
StyI C'CwwG_G
Cuts at: 374 1540 1894 374
Size: 1166 354 2886
Fragments arranged by size:
2886 1166 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 360 566 801 1989 2122 2581 2790 360
Size: 206 235 1188 133 459 209 1976
Fragments arranged by size:
1976 1188 459 235 209 206 133
TatI w'GTAC_w
Cuts at: 365 3921 365
Size: 3556 850
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 155 187 3923 3955 155
Size: 32 3736 32 606
Fragments arranged by size:
3736 606 32 32
Tth111I GACn'n_nGTC
Cuts at: 502 2203 502
Size: 1701 2705
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 354 1901 354
Size: 1547 2859
XhoI C'TCGA_G
Cuts at: 222 222
Size: 4406
XmaI C'CCGG_G
Cuts at: 1426 1426
Size: 4406
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PpuMI PsiI PsrI SanDI
SexAI SfiI SnaBI SrfI SspI SwaI XmnI ZraI