Restriction map for
pF1KB6595
(Circular) (Six-base) MAPSORT of: pF1KB6595.seq Check: 902 from: 1 to: 4253
pF1KB6595 4253 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1291 1291
Size: 4253
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 2078 2510 3257 3525 167
Size: 1911 432 747 268 895
Fragments arranged by size:
1911 895 747 432 268
AflIII A'CryG_T
Cuts at: 1605 2709 3686 1605
Size: 1104 977 2172
Fragments arranged by size:
2172 1104 977
AgeI A'CCGG_T
Cuts at: 1183 2641 1183
Size: 1458 2795
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 446 478 446
Size: 32 4221
AlwNI CAG_nnn'CTG
Cuts at: 1558 3125 1558
Size: 1567 2686
ApaLI G'TGCA_C
Cuts at: 3023 3023
Size: 4253
ApoI r'AATT_y
Cuts at: 509 1067 1231 1257 3606 3862 509
Size: 558 164 26 2349 256 900
Fragments arranged by size:
2349 900 558 256 164 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 848 1273 848
Size: 425 3828
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 231 264 231
Size: 33 4220
BamHI G'GATC_C
Cuts at: 231 1278 231
Size: 1047 3206
BanI G'GyrC_C
Cuts at: 1269 1683 1931 1966 1269
Size: 414 248 35 3556
Fragments arranged by size:
3556 414 248 35
BanII G_rGCy'C
Cuts at: 1267 2297 1267
Size: 1030 3223
BbeI G_GCGC'C
Cuts at: 1687 1935 1687
Size: 248 4005
BbsI GAAGACnn'nnnn_
Cuts at: 358 1115 358
Size: 757 3496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1001 1035 1001
Size: 34 4219
BciVI GTATCCnnnnn_n'
Cuts at: 232 2151 2912 4253 232
Size: 1919 761 1341 232
Fragments arranged by size:
1919 1341 761 232
BclI T'GATC_A
Cuts at: 1773 1773
Size: 4253
BfrBI ATG'CAT
Cuts at: 649 649
Size: 4253
BglII A'GATC_T
Cuts at: 1768 3805 1768
Size: 2037 2216
BlpI GC'TnA_GC
Cuts at: 1365 1365
Size: 4253
Bme1580I G_kGCm'C
Cuts at: 1878 1971 3027 1878
Size: 93 1056 3104
Fragments arranged by size:
3104 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1465 1546 1881 4025 1465
Size: 81 335 2144 1693
Fragments arranged by size:
2144 1693 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 2308 2800 3098 1429
Size: 879 492 298 2584
Fragments arranged by size:
2584 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3957 3957
Size: 4253
BsaAI yAC'GTr
Cuts at: 1606 1606
Size: 4253
BsaBI GATnn'nnATC
Cuts at: 6 1767 3804 6
Size: 1761 2037 455
Fragments arranged by size:
2037 1761 455
BsaHI Gr'CG_yC
Cuts at: 1684 1932 2634 4130 1684
Size: 248 702 1496 1807
Fragments arranged by size:
1807 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1183 1442 1963 2641 2915 3062 3473 1183
Size: 259 521 678 274 147 411 1963
Fragments arranged by size:
1963 678 521 411 274 259 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 200 200
Size: 4253
BseYI C'CCAG_C
Cuts at: 1097 1678 2436 3013 3509 1097
Size: 581 758 577 496 1841
Fragments arranged by size:
1841 758 581 577 496
BsiEI CG_ry'CG
Cuts at: 104 1795 1841 3049 104
Size: 1691 46 1208 1308
Fragments arranged by size:
1691 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1267 2045 2235 3027 1267
Size: 778 190 792 2493
Fragments arranged by size:
2493 792 778 190
BsmI GAATG_Cn'
Cuts at: 304 304
Size: 4253
Bsp1286I G_dGCh'C
Cuts at: 1267 1878 1971 2045 2235 2297 3027 1267
Size: 611 93 74 190 62 730 2493
Fragments arranged by size:
2493 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3473 3473
Size: 4253
BspHI T'CATG_A
Cuts at: 3429 3429
Size: 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1303 1517 1819 2200 1303
Size: 214 302 381 3356
Fragments arranged by size:
3356 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2545 2599 4083 4248 50
Size: 2495 54 1484 165 55
Fragments arranged by size:
2495 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 268 448 2165 268
Size: 180 1717 2356
Fragments arranged by size:
2356 1717 180
BsrFI r'CCGG_y
Cuts at: 1183 2251 2432 2641 1183
Size: 1068 181 209 2795
Fragments arranged by size:
2795 1068 209 181
BssSI C'ACGA_G
Cuts at: 2524 2882 2524
Size: 358 3895
BstAPI GCAn_nnn'nTGC
Cuts at: 1630 1630
Size: 4253
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstYI r'GATC_y
Cuts at: 1 231 778 1278 1745 1768 2103 2349 3361
Size: 230 547 500 467 23 335 246 1012
Cuts at: 3361 3447 3459 3805 1
Size: 86 12 346 449
Fragments arranged by size:
1012 547 500 467 449 346 335 246
230 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 212 212
Size: 4253
BtgI C'CryG_G
Cuts at: 107 436 107
Size: 329 3924
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2203 2384 2547 2203
Size: 181 163 3909
Fragments arranged by size:
3909 181 163
BtsI GCAGTG_nn'
Cuts at: 1496 1496
Size: 4253
DraI TTT'AAA
Cuts at: 1252 1252
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 1959 2817 1959
Size: 858 3395
EaeI y'GGCC_r
Cuts at: 1838 2012 2403 2430 3775 1838
Size: 174 391 27 1345 2316
Fragments arranged by size:
2316 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1838 1838
Size: 4253
EarI CTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 2771 2917 4063 2461
Size: 310 146 1146 2651
Fragments arranged by size:
2651 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1265 1265
Size: 4253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 2078 2510 3257 3525 167
Size: 1911 432 747 268 895
Fragments arranged by size:
1911 895 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 587 587
Size: 4253
EcoO109I rG'GnC_Cy
Cuts at: 895 1392 895
Size: 497 3756
EcoRI G'AATT_C
Cuts at: 509 1257 509
Size: 748 3505
EcoRV GAT'ATC
Cuts at: 497 497
Size: 4253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 4253
FspI TGC'GCA
Cuts at: 2034 2034
Size: 4253
HaeII r_GCGC'y
Cuts at: 1687 1935 2957 3936 1687
Size: 248 1022 979 2004
Fragments arranged by size:
2004 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 216 248 2095 2125 2127 2157 216
Size: 32 1847 30 2 30 2312
Fragments arranged by size:
2312 1847 32 30 30 2
HincII GTy'rAC
Cuts at: 204 1292 204
Size: 1088 3165
HpaI GTT'AAC
Cuts at: 204 204
Size: 4253
KasI G'GCGC_C
Cuts at: 1683 1931 1683
Size: 248 4005
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MfeI C'AATT_G
Cuts at: 552 552
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2924 3108 2924
Size: 184 4069
MscI TGG'CCA
Cuts at: 2014 2014
Size: 4253
MslI CAynn'nnrTG
Cuts at: 441 654 2242 2369 441
Size: 213 1588 127 2325
Fragments arranged by size:
2325 1588 213 127
MspA1I CmG'CkG
Cuts at: 1364 1678 2038 3051 3296 1364
Size: 314 360 1013 245 2321
Fragments arranged by size:
2321 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2434 2434
Size: 4253
NarI GG'CG_CC
Cuts at: 1684 1932 1684
Size: 248 4005
NcoI C'CATG_G
Cuts at: 107 436 107
Size: 329 3924
NgoMIV G'CCGG_C
Cuts at: 2432 2432
Size: 4253
NsiI A_TGCA'T
Cuts at: 651 651
Size: 4253
NspI r_CATG'y
Cuts at: 1306 1806 2713 1306
Size: 500 907 2846
Fragments arranged by size:
2846 907 500
PciI A'CATG_T
Cuts at: 2709 2709
Size: 4253
PfoI T'CCnGG_A
Cuts at: 234 234
Size: 4253
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4221
PpuMI rG'GwC_Cy
Cuts at: 895 895
Size: 4253
PstI C_TGCA'G
Cuts at: 1300 1985 1300
Size: 685 3568
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 1678 2038 1678
Size: 360 3893
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 1267 1267
Size: 4253
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SfcI C'TryA_G
Cuts at: 33 965 1296 1981 2974 3165 33
Size: 932 331 685 993 191 1121
Fragments arranged by size:
1121 993 932 685 331 191
SfoI GGC'GCC
Cuts at: 1685 1933 1685
Size: 248 4005
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 1275 1275
Size: 4253
SmlI C'TyrA_G
Cuts at: 1408 2323 2815 3077 1408
Size: 915 492 262 2584
Fragments arranged by size:
2584 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
SspI AAT'ATT
Cuts at: 562 1047 1166 562
Size: 485 119 3649
Fragments arranged by size:
3649 485 119
StyI C'CwwG_G
Cuts at: 107 436 953 1387 1741 107
Size: 329 517 434 354 2619
Fragments arranged by size:
2619 517 434 354 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1836 1969 2428 2637 1836
Size: 133 459 209 3452
Fragments arranged by size:
3452 459 209 133
TatI w'GTAC_w
Cuts at: 172 594 3768 172
Size: 422 3174 657
Fragments arranged by size:
3174 657 422
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 4221
Tth111I GACn'n_nGTC
Cuts at: 2050 2050
Size: 4253
XbaI T'CTAG_A
Cuts at: 1284 1284
Size: 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1748 1748
Size: 4253
XmaI C'CCGG_G
Cuts at: 1273 1273
Size: 4253
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BbvCI BglI BmgBI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII FseI FspAI HindIII NdeI
NheI NotI NruI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI