Restriction map for
pF1KB6586
(Circular) (Six-base) MAPSORT of: pF1KB6586.seq Check: 120 from: 1 to: 4331
pF1KB6586 4331 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 927 927
Size: 4331
AccI GT'mk_AC
Cuts at: 524 1369 524
Size: 845 3486
Acc65I G'GTAC_C
Cuts at: 804 1347 804
Size: 543 3788
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 2156 2588 3335 3603 436
Size: 1720 432 747 268 1164
Fragments arranged by size:
1720 1164 747 432 268
AflII C'TTAA_G
Cuts at: 670 979 670
Size: 309 4022
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 388 388
Size: 4331
AlwNI CAG_nnn'CTG
Cuts at: 381 1636 3203 381
Size: 1255 1567 1509
Fragments arranged by size:
1567 1509 1255
ApaLI G'TGCA_C
Cuts at: 1150 3101 1150
Size: 1951 2380
ApoI r'AATT_y
Cuts at: 1021 1335 3684 3940 1021
Size: 314 2349 256 1412
Fragments arranged by size:
2349 1412 314 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 179 1003 1351 179
Size: 824 348 3159
Fragments arranged by size:
3159 824 348
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 217 757 804 1347 1761 2009 2044 217
Size: 540 47 543 414 248 35 2504
Fragments arranged by size:
2504 543 540 414 248 47 35
BanII G_rGCy'C
Cuts at: 1039 1345 2375 1039
Size: 306 1030 2995
Fragments arranged by size:
2995 1030 306
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 562 1077 562
Size: 515 3816
BbvCI CC'TCA_GC
Cuts at: 967 967
Size: 4331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 670 675 704 709 90
Size: 34 546 5 29 5 3712
Fragments arranged by size:
3712 546 34 29 5 5
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 698 1851 698
Size: 1153 3178
BglI GCCn_nnn'nGGC
Cuts at: 226 226
Size: 4331
BglII A'GATC_T
Cuts at: 1846 3883 1846
Size: 2037 2294
BlpI GC'TnA_GC
Cuts at: 487 1443 487
Size: 956 3375
Bme1580I G_kGCm'C
Cuts at: 1154 1956 2049 3105 1154
Size: 802 93 1056 2380
Fragments arranged by size:
2380 1056 802 93
BmrI ACTGGGnnnn_n'
Cuts at: 911 1058 1543 1624 1959 4103 911
Size: 147 485 81 335 2144 1139
Fragments arranged by size:
2144 1139 485 335 147 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 403 1069 388
Size: 15 666 3650
Fragments arranged by size:
3650 666 15
Bpu10I CC'TnA_GC
Cuts at: 967 967
Size: 4331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2386 2878 3176 1507
Size: 879 492 298 2662
Fragments arranged by size:
2662 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4035 4035
Size: 4331
BsaAI yAC'GTr
Cuts at: 780 1684 780
Size: 904 3427
BsaBI GATnn'nnATC
Cuts at: 6 376 830 1845 3882 6
Size: 370 454 1015 2037 455
Fragments arranged by size:
2037 1015 455 454 370
BsaHI Gr'CG_yC
Cuts at: 1762 2010 2712 4208 1762
Size: 248 702 1496 1885
Fragments arranged by size:
1885 1496 702 248
BsaWI w'CCGG_w
Cuts at: 848 1520 2041 2719 2993 3140 3551 848
Size: 672 521 678 274 147 411 1628
Fragments arranged by size:
1628 678 672 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 112 112
Size: 4331
BseYI C'CCAG_C
Cuts at: 232 289 355 1756 2514 3091 3587 232
Size: 57 66 1401 758 577 496 976
Fragments arranged by size:
1401 976 758 577 496 66 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 4331
BsiEI CG_ry'CG
Cuts at: 104 695 1873 1919 3127 104
Size: 591 1178 46 1208 1308
Fragments arranged by size:
1308 1208 1178 591 46
BsiHKAI G_wGCw'C
Cuts at: 1154 1345 2123 2313 3105 1154
Size: 191 778 190 792 2380
Fragments arranged by size:
2380 792 778 191 190
Bsp1286I G_dGCh'C
Cuts at: 1039 1154 1345 1956 2049 2123 2313 2375 3105
Size: 115 191 611 93 74 190 62 730
Cuts at: 3105 1039
Size: 2265
Fragments arranged by size:
2265 730 611 191 190 115 93 74
62
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 536 927 1381 1595 1897 2278 536
Size: 391 454 214 302 381 2589
Fragments arranged by size:
2589 454 391 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2623 2677 4161 4326 50
Size: 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 504 693 897 2243 504
Size: 189 204 1346 2592
Fragments arranged by size:
2592 1346 204 189
BsrFI r'CCGG_y
Cuts at: 2329 2510 2719 2329
Size: 181 209 3941
Fragments arranged by size:
3941 209 181
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 1708 1708
Size: 4331
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 333 333
Size: 4331
BstYI r'GATC_y
Cuts at: 1 377 1356 1823 1846 2181 2427 3439 3525
Size: 376 979 467 23 335 246 1012 86
Cuts at: 3525 3537 3883 1
Size: 12 346 449
Fragments arranged by size:
1012 979 467 449 376 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 149 149
Size: 4331
BtgI C'CryG_G
Cuts at: 221 896 1202 221
Size: 675 306 3350
Fragments arranged by size:
3350 675 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 1574 1574
Size: 4331
DraI TTT'AAA
Cuts at: 723 1330 723
Size: 607 3724
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 335 1916 2090 2481 2508 3853 335
Size: 1581 174 391 27 1345 813
Fragments arranged by size:
1581 1345 813 391 174 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 121 187 1306 2354 2564 121
Size: 66 1119 1048 210 1888
Fragments arranged by size:
1888 1119 1048 210 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2539 2849 2995 4141 2539
Size: 310 146 1146 2729
Fragments arranged by size:
2729 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1343 1343
Size: 4331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 403 436 1069 2156 2588 3335 3603 388
Size: 15 33 633 1087 432 747 268 1116
Fragments arranged by size:
1116 1087 747 633 432 268 33 15
EcoO109I rG'GnC_Cy
Cuts at: 155 204 269 387 876 1265 1470 155
Size: 49 65 118 489 389 205 3016
Fragments arranged by size:
3016 489 389 205 118 65 49
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 1765 2013 3035 4014 1765
Size: 248 1022 979 2082
Fragments arranged by size:
2082 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2173 2203 2205 2235 2173
Size: 30 2 30 4269
Fragments arranged by size:
4269 30 30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
HindIII A'AGCT_T
Cuts at: 741 741
Size: 4331
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 808 1351 808
Size: 543 3788
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3186 3002
Size: 184 4147
MscI TGG'CCA
Cuts at: 337 2092 337
Size: 1755 2576
MslI CAynn'nnrTG
Cuts at: 215 325 370 2320 2447 215
Size: 110 45 1950 127 2099
Fragments arranged by size:
2099 1950 127 110 45
MspA1I CmG'CkG
Cuts at: 283 486 1047 1195 1442 1756 2116 3129 3374
Size: 203 561 148 247 314 360 1013 245
Cuts at: 3374 283
Size: 1240
Fragments arranged by size:
1240 1013 561 360 314 247 245 203
148
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NspI r_CATG'y
Cuts at: 1384 1884 2791 1384
Size: 500 907 2924
Fragments arranged by size:
2924 907 500
PasI CC'CwG_GG
Cuts at: 348 348
Size: 4331
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PfoI T'CCnGG_A
Cuts at: 380 842 380
Size: 462 3869
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PpuMI rG'GwC_Cy
Cuts at: 204 876 204
Size: 672 3659
PspXI vC'TCGA_Gb
Cuts at: 1003 1003
Size: 4331
PstI C_TGCA'G
Cuts at: 533 606 865 1378 2063 533
Size: 73 259 513 685 2801
Fragments arranged by size:
2801 685 513 259 73
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 486 1047 1756 2116 486
Size: 561 709 360 2701
Fragments arranged by size:
2701 709 561 360
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 1345 1345
Size: 4331
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SanDI GG'GwC_CC
Cuts at: 876 876
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 121 2354 2564 121
Size: 2233 210 1888
Fragments arranged by size:
2233 1888 210
SbfI CC_TGCA'GG
Cuts at: 533 865 1378 533
Size: 332 513 3486
Fragments arranged by size:
3486 513 332
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SexAI A'CCwGG_T
Cuts at: 515 515
Size: 4331
SfcI C'TryA_G
Cuts at: 33 264 529 602 861 1374 2059 3052 3243
Size: 231 265 73 259 513 685 993 191
Cuts at: 3243 33
Size: 1121
Fragments arranged by size:
1121 993 685 513 265 259 231 191
73
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 181 1353 181
Size: 1172 3159
SmlI C'TyrA_G
Cuts at: 670 979 1003 1486 2401 2893 3155 670
Size: 309 24 483 915 492 262 1846
Fragments arranged by size:
1846 915 492 483 309 262 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
SspI AAT'ATT
Cuts at: 1214 1214
Size: 4331
StyI C'CwwG_G
Cuts at: 272 885 1305 1465 1819 272
Size: 613 420 160 354 2784
Fragments arranged by size:
2784 613 420 354 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 775 1914 2047 2506 2715 775
Size: 1139 133 459 209 2391
Fragments arranged by size:
2391 1139 459 209 133
TatI w'GTAC_w
Cuts at: 3846 3846
Size: 4331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4299
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 345 472 1826 345
Size: 127 1354 2850
Fragments arranged by size:
2850 1354 127
XhoI C'TCGA_G
Cuts at: 1003 1003
Size: 4331
XmaI C'CCGG_G
Cuts at: 179 1351 179
Size: 1172 3159
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PsrI SacII SfiI SgrAI SnaBI SrfI StuI
SwaI XmnI ZraI