Restriction map for
pF1KB6583
(Circular) (Six-base) MAPSORT of: pF1KB6583.seq Check: 1149 from: 1 to: 4244
pF1KB6583 4244 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1176 1176
Size: 4244
AatII G_ACGT'C
Cuts at: 1022 1022
Size: 4244
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2501 3248 3516 2069
Size: 432 747 268 2797
Fragments arranged by size:
2797 747 432 268
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AleI CACnn'nnGTG
Cuts at: 202 361 202
Size: 159 4085
AlwNI CAG_nnn'CTG
Cuts at: 633 1549 3116 633
Size: 916 1567 1761
Fragments arranged by size:
1761 1567 916
ApaI G_GGCC'C
Cuts at: 352 991 352
Size: 639 3605
ApaLI G'TGCA_C
Cuts at: 3014 3014
Size: 4244
ApoI r'AATT_y
Cuts at: 219 1248 3597 3853 219
Size: 1029 2349 256 610
Fragments arranged by size:
2349 1029 610 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 179 224 955 1207 1264 179
Size: 45 731 252 57 3159
Fragments arranged by size:
3159 731 252 57 45
AvrII C'CTAG_G
Cuts at: 1134 1134
Size: 4244
BamHI G'GATC_C
Cuts at: 713 1269 713
Size: 556 3688
BanI G'GyrC_C
Cuts at: 436 1213 1260 1674 1922 1957 436
Size: 777 47 414 248 35 2723
Fragments arranged by size:
2723 777 414 248 47 35
BanII G_rGCy'C
Cuts at: 241 352 964 991 1258 2288 241
Size: 111 612 27 267 1030 2197
Fragments arranged by size:
2197 1030 612 267 111 27
BbeI G_GCGC'C
Cuts at: 440 1217 1678 1926 440
Size: 777 461 248 2758
Fragments arranged by size:
2758 777 461 248
BciVI GTATCCnnnnn_n'
Cuts at: 2142 2903 4244 2142
Size: 761 1341 2142
Fragments arranged by size:
2142 1341 761
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BglI GCCn_nnn'nGGC
Cuts at: 959 959
Size: 4244
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 1356 1356
Size: 4244
Bme1580I G_kGCm'C
Cuts at: 322 352 991 1869 1962 3018 322
Size: 30 639 878 93 1056 1548
Fragments arranged by size:
1548 1056 878 639 93 30
BmgBI CAC'GTC
Cuts at: 923 923
Size: 4244
BmrI ACTGGGnnnn_n'
Cuts at: 832 1456 1537 1872 4016 832
Size: 624 81 335 2144 1060
Fragments arranged by size:
2144 1060 624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 562 739 843 140
Size: 422 177 104 3541
Fragments arranged by size:
3541 422 177 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1420 2299 2791 3089 727
Size: 693 879 492 298 1882
Fragments arranged by size:
1882 879 693 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 565 3948 565
Size: 3383 861
BsaAI yAC'GTr
Cuts at: 1597 1597
Size: 4244
BsaBI GATnn'nnATC
Cuts at: 6 1758 3795 6
Size: 1752 2037 455
Fragments arranged by size:
2037 1752 455
BsaHI Gr'CG_yC
Cuts at: 112 437 1019 1214 1675 1923 2625 4121 112
Size: 325 582 195 461 248 702 1496 235
Fragments arranged by size:
1496 702 582 461 325 248 235 195
BsaWI w'CCGG_w
Cuts at: 1433 1954 2632 2906 3053 3464 1433
Size: 521 678 274 147 411 2213
Fragments arranged by size:
2213 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1069 1072 1099 1108 1138 1069
Size: 3 27 9 30 4175
Fragments arranged by size:
4175 30 27 9 3
BseYI C'CCAG_C
Cuts at: 351 984 1669 2427 3004 3500 351
Size: 633 685 758 577 496 1095
Fragments arranged by size:
1095 758 685 633 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 1162
Size: 4244
BsiEI CG_ry'CG
Cuts at: 104 522 872 1786 1832 3040 104
Size: 418 350 914 46 1208 1308
Fragments arranged by size:
1308 1208 914 418 350 46
BsiHKAI G_wGCw'C
Cuts at: 1258 2036 2226 3018 1258
Size: 778 190 792 2484
Fragments arranged by size:
2484 792 778 190
BsmI GAATG_Cn'
Cuts at: 259 259
Size: 4244
Bsp1286I G_dGCh'C
Cuts at: 241 322 352 964 991 1258 1869 1962 2036
Size: 81 30 612 27 267 611 93 74
Cuts at: 2036 2226 2288 3018 241
Size: 190 62 730 1467
Fragments arranged by size:
1467 730 612 611 267 190 93 81
74 62 30 27
BspEI T'CCGG_A
Cuts at: 3464 3464
Size: 4244
BspHI T'CATG_A
Cuts at: 3420 3420
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 506 1176 1294 1508 1810 2191 506
Size: 670 118 214 302 381 2559
Fragments arranged by size:
2559 670 381 302 214 118
BsrBI CCG'CTC
Cuts at: 50 1233 2536 2590 4074 4239 50
Size: 1183 1303 54 1484 165 55
Fragments arranged by size:
1484 1303 1183 165 55 54
BsrDI GCAATG_nn'
Cuts at: 461 2156 461
Size: 1695 2549
BsrFI r'CCGG_y
Cuts at: 556 2242 2423 2632 556
Size: 1686 181 209 2168
Fragments arranged by size:
2168 1686 209 181
BssSI C'ACGA_G
Cuts at: 673 2515 2873 673
Size: 1842 358 2044
Fragments arranged by size:
2044 1842 358
BstAPI GCAn_nnn'nTGC
Cuts at: 463 1621 463
Size: 1158 3086
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstEII G'GTnAC_C
Cuts at: 969 969
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 363 453 363
Size: 90 4154
BstYI r'GATC_y
Cuts at: 1 656 713 818 833 1269 1736 1759 2094
Size: 655 57 105 15 436 467 23 335
Cuts at: 2094 2340 3352 3438 3450 3796 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 655 467 449 436 346 335 246
105 86 57 23 15 12
BtgI C'CryG_G
Cuts at: 107 197 965 107
Size: 90 768 3386
Fragments arranged by size:
3386 768 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 209 320 1487 209
Size: 111 1167 2966
Fragments arranged by size:
2966 1167 111
DraI TTT'AAA
Cuts at: 779 1243 779
Size: 464 3780
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 443 519 680 869 1829 2003 2394 2421 3766
Size: 76 161 189 960 174 391 27 1345
Cuts at: 3766 443
Size: 921
Fragments arranged by size:
1345 960 921 391 189 174 161 76
27
EagI C'GGCC_G
Cuts at: 519 869 1829 519
Size: 350 960 2934
Fragments arranged by size:
2934 960 350
EarI CTCTTCn'nnn_
Cuts at: 284 1057 2267 2477 284
Size: 773 1210 210 2051
Fragments arranged by size:
2051 1210 773 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 220 2452 2762 2908 4054 220
Size: 2232 310 146 1146 410
Fragments arranged by size:
2232 1146 410 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 562 739 843 2069 2501 3248 3516 140
Size: 422 177 104 1226 432 747 268 868
Fragments arranged by size:
1226 868 747 432 422 268 177 104
EcoO109I rG'GnC_Cy
Cuts at: 988 1383 988
Size: 395 3849
EcoRI G'AATT_C
Cuts at: 219 1248 219
Size: 1029 3215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 790 2263 790
Size: 1473 2771
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 440 1217 1678 1926 2948 3927 440
Size: 777 461 248 1022 979 757
Fragments arranged by size:
1022 979 777 757 461 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 325 357 582 614 907 939 2086 2116 2118
Size: 32 225 32 293 32 1147 30 2
Cuts at: 2118 2148 325
Size: 30 2421
Fragments arranged by size:
2421 1147 293 225 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
KasI G'GCGC_C
Cuts at: 436 1213 1674 1922 436
Size: 777 461 248 2758
Fragments arranged by size:
2758 777 461 248
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3099 2915
Size: 184 4060
MscI TGG'CCA
Cuts at: 445 2005 445
Size: 1560 2684
MslI CAynn'nnrTG
Cuts at: 202 361 451 2233 2360 202
Size: 159 90 1782 127 2086
Fragments arranged by size:
2086 1782 159 127 90
MspA1I CmG'CkG
Cuts at: 189 540 685 1355 1669 2029 3042 3287 189
Size: 351 145 670 314 360 1013 245 1146
Fragments arranged by size:
1146 1013 670 360 351 314 245 145
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 4244
NarI GG'CG_CC
Cuts at: 437 1214 1675 1923 437
Size: 777 461 248 2758
Fragments arranged by size:
2758 777 461 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4244
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 4244
NspI r_CATG'y
Cuts at: 1297 1797 2704 1297
Size: 500 907 2837
Fragments arranged by size:
2837 907 500
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PflMI CCAn_nnn'nTGG
Cuts at: 678 710 984 678
Size: 32 274 3938
Fragments arranged by size:
3938 274 32
PfoI T'CCnGG_A
Cuts at: 156 626 659 716 156
Size: 470 33 57 3684
Fragments arranged by size:
3684 470 57 33
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 4212
PspOMI G'GGCC_C
Cuts at: 348 987 348
Size: 639 3605
PstI C_TGCA'G
Cuts at: 1291 1976 1291
Size: 685 3559
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 540 1669 2029 540
Size: 1129 360 2755
Fragments arranged by size:
2755 1129 360
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 214 1287 1972 2965 3156 33
Size: 181 1073 685 993 191 1121
Fragments arranged by size:
1121 1073 993 685 191 181
SfoI GGC'GCC
Cuts at: 438 1215 1676 1924 438
Size: 777 461 248 2758
Fragments arranged by size:
2758 777 461 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 226 1266 226
Size: 1040 3204
SmlI C'TyrA_G
Cuts at: 179 742 1399 2314 2806 3068 179
Size: 563 657 915 492 262 1355
Fragments arranged by size:
1355 915 657 563 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
StyI C'CwwG_G
Cuts at: 107 344 689 1134 1378 1732 107
Size: 237 345 445 244 354 2619
Fragments arranged by size:
2619 445 354 345 244 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1827 1960 2419 2628 1827
Size: 133 459 209 3443
Fragments arranged by size:
3443 459 209 133
TatI w'GTAC_w
Cuts at: 3759 3759
Size: 4244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4212
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 714 1739 714
Size: 1025 3219
XhoI C'TCGA_G
Cuts at: 179 179
Size: 4244
XmaI C'CCGG_G
Cuts at: 224 1264 224
Size: 1040 3204
ZraI GAC'GTC
Cuts at: 1020 1020
Size: 4244
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI BaeI BbsI
BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI