Restriction map for
pF1KB6581
(Circular) (Six-base) MAPSORT of: pF1KB6581.seq Check: 9498 from: 1 to: 4244
pF1KB6581 4244 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AatII G_ACGT'C
Cuts at: 1073 1073
Size: 4244
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 801 4084 801
Size: 3283 961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 2069 2501 3248 3516 322
Size: 1747 432 747 268 1050
Fragments arranged by size:
1747 1050 747 432 268
AflII C'TTAA_G
Cuts at: 1062 1062
Size: 4244
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AlwNI CAG_nnn'CTG
Cuts at: 1549 3116 1549
Size: 1567 2677
ApaI G_GGCC'C
Cuts at: 495 495
Size: 4244
ApaLI G'TGCA_C
Cuts at: 3014 3014
Size: 4244
ApoI r'AATT_y
Cuts at: 607 859 1039 1078 1248 3597 3853 607
Size: 252 180 39 170 2349 256 998
Fragments arranged by size:
2349 998 256 252 180 170 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 224 413 488 1264 224
Size: 189 75 776 3204
Fragments arranged by size:
3204 776 189 75
BamHI G'GATC_C
Cuts at: 1050 1269 1050
Size: 219 4025
BanI G'GyrC_C
Cuts at: 1260 1674 1922 1957 1260
Size: 414 248 35 3547
Fragments arranged by size:
3547 414 248 35
BanII G_rGCy'C
Cuts at: 495 1258 2288 495
Size: 763 1030 2451
Fragments arranged by size:
2451 1030 763
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BbsI GAAGACnn'nnnn_
Cuts at: 714 714
Size: 4244
BciVI GTATCCnnnnn_n'
Cuts at: 1191 2142 2903 4244 1191
Size: 951 761 1341 1191
Fragments arranged by size:
1341 1191 951 761
BclI T'GATC_A
Cuts at: 383 1764 383
Size: 1381 2863
BglI GCCn_nnn'nGGC
Cuts at: 499 499
Size: 4244
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 1356 1356
Size: 4244
Bme1580I G_kGCm'C
Cuts at: 495 1869 1962 3018 495
Size: 1374 93 1056 1721
Fragments arranged by size:
1721 1374 1056 93
BmgBI CAC'GTC
Cuts at: 1191 1191
Size: 4244
BmrI ACTGGGnnnn_n'
Cuts at: 625 1456 1537 1872 4016 625
Size: 831 81 335 2144 853
Fragments arranged by size:
2144 853 831 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 837 1015 1204 1420 2299 2791 3089 837
Size: 178 189 216 879 492 298 1992
Fragments arranged by size:
1992 879 492 298 216 189 178
BsaI GGTCTCn'nnnn_
Cuts at: 3948 3948
Size: 4244
BsaAI yAC'GTr
Cuts at: 1597 1597
Size: 4244
BsaBI GATnn'nnATC
Cuts at: 6 313 1758 3795 6
Size: 307 1445 2037 455
Fragments arranged by size:
2037 1445 455 307
BsaHI Gr'CG_yC
Cuts at: 600 1070 1675 1923 2625 4121 600
Size: 470 605 248 702 1496 723
Fragments arranged by size:
1496 723 702 605 470 248
BsaWI w'CCGG_w
Cuts at: 1097 1433 1954 2632 2906 3053 3464 1097
Size: 336 521 678 274 147 411 1877
Fragments arranged by size:
1877 678 521 411 336 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 1012 367
Size: 645 3599
BseYI C'CCAG_C
Cuts at: 267 1669 2427 3004 3500 267
Size: 1402 758 577 496 1011
Fragments arranged by size:
1402 1011 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1786 1832 3040 104
Size: 1682 46 1208 1308
Fragments arranged by size:
1682 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1258 2036 2226 3018 1258
Size: 778 190 792 2484
Fragments arranged by size:
2484 792 778 190
BsmI GAATG_Cn'
Cuts at: 591 591
Size: 4244
BsmBI CGTCTCn'nnnn_
Cuts at: 1061 1061
Size: 4244
Bsp1286I G_dGCh'C
Cuts at: 495 1258 1869 1962 2036 2226 2288 3018 495
Size: 763 611 93 74 190 62 730 1721
Fragments arranged by size:
1721 763 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1097 3464 1097
Size: 2367 1877
BspHI T'CATG_A
Cuts at: 3420 3420
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1294 1508 1810 2191 1294
Size: 214 302 381 3347
Fragments arranged by size:
3347 381 302 214
BsrBI CCG'CTC
Cuts at: 50 284 2536 2590 4074 4239 50
Size: 234 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 234 165 55 54
BsrDI GCAATG_nn'
Cuts at: 960 2156 960
Size: 1196 3048
BsrFI r'CCGG_y
Cuts at: 596 2242 2423 2632 596
Size: 1646 181 209 2208
Fragments arranged by size:
2208 1646 209 181
BssHII G'CGCG_C
Cuts at: 342 344 346 342
Size: 2 2 4240
Fragments arranged by size:
4240 2 2
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 1230 1621 1230
Size: 391 3853
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 460 460
Size: 4244
BstYI r'GATC_y
Cuts at: 1 257 1050 1269 1736 1759 2094 2340 3352
Size: 256 793 219 467 23 335 246 1012
Cuts at: 3352 3438 3450 3796 1
Size: 86 12 346 449
Fragments arranged by size:
1012 793 467 449 346 335 256 246
219 86 23 12
BtgI C'CryG_G
Cuts at: 107 321 497 107
Size: 214 176 3854
Fragments arranged by size:
3854 214 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 1056 1148 1487 1056
Size: 92 339 3813
Fragments arranged by size:
3813 339 92
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 1829 2003 2394 2421 3766 1829
Size: 174 391 27 1345 2307
Fragments arranged by size:
2307 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 349 1162 2452 2762 2908 4054 127
Size: 222 813 1290 310 146 1146 317
Fragments arranged by size:
1290 1146 813 317 310 222 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 2069 2501 3248 3516 322
Size: 1747 432 747 268 1050
Fragments arranged by size:
1747 1050 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 492 1383 492
Size: 891 3353
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 312 1005 2263 312
Size: 693 1258 2293
Fragments arranged by size:
2293 1258 693
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 1678 1926 2948 3927 1678
Size: 248 1022 979 1995
Fragments arranged by size:
1995 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2086 2116 2118 2148 2086
Size: 30 2 30 4182
Fragments arranged by size:
4182 30 30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
HindIII A'AGCT_T
Cuts at: 421 421
Size: 4244
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3099 2915
Size: 184 4060
MscI TGG'CCA
Cuts at: 2005 2005
Size: 4244
MslI CAynn'nnrTG
Cuts at: 2233 2360 2233
Size: 127 4117
MspA1I CmG'CkG
Cuts at: 575 1355 1669 2029 3042 3287 575
Size: 780 314 360 1013 245 1532
Fragments arranged by size:
1532 1013 780 360 314 245
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 4244
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NcoI C'CATG_G
Cuts at: 107 497 107
Size: 390 3854
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 4244
NspI r_CATG'y
Cuts at: 1297 1797 2704 1297
Size: 500 907 2837
Fragments arranged by size:
2837 907 500
PasI CC'CwG_GG
Cuts at: 434 434
Size: 4244
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PflMI CCAn_nnn'nTGG
Cuts at: 513 513
Size: 4244
PfoI T'CCnGG_A
Cuts at: 638 638
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 4212
PshAI GACnn'nnGTC
Cuts at: 599 599
Size: 4244
PspOMI G'GGCC_C
Cuts at: 491 491
Size: 4244
PstI C_TGCA'G
Cuts at: 1291 1976 1291
Size: 685 3559
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 1669 2029 1669
Size: 360 3884
RsrII CG'GwC_CG
Cuts at: 131 2439 131
Size: 2308 1936
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SexAI A'CCwGG_T
Cuts at: 866 866
Size: 4244
SfcI C'TryA_G
Cuts at: 33 372 1234 1287 1972 2965 3156 33
Size: 339 862 53 685 993 191 1121
Fragments arranged by size:
1121 993 862 685 339 191 53
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 490 1266 490
Size: 776 3468
SmlI C'TyrA_G
Cuts at: 413 852 994 1062 1219 1399 2314 2806 3068
Size: 439 142 68 157 180 915 492 262
Cuts at: 3068 413
Size: 1589
Fragments arranged by size:
1589 915 492 439 262 180 157 142
68
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
SspI AAT'ATT
Cuts at: 634 634
Size: 4244
StyI C'CwwG_G
Cuts at: 107 200 497 507 1378 1732 107
Size: 93 297 10 871 354 2619
Fragments arranged by size:
2619 871 354 297 93 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 607 1827 1960 2419 2628 607
Size: 1220 133 459 209 2223
Fragments arranged by size:
2223 1220 459 209 133
TatI w'GTAC_w
Cuts at: 3759 3759
Size: 4244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4212
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1739 1739
Size: 4244
XhoI C'TCGA_G
Cuts at: 413 413
Size: 4244
XmaI C'CCGG_G
Cuts at: 488 1264 488
Size: 776 3468
ZraI GAC'GTC
Cuts at: 1071 1071
Size: 4244
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BsaXI
BsgI BsiWI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PpuMI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XmnI