Restriction map for
pF1KB6577
(Circular) (Six-base) MAPSORT of: pF1KB6577.seq Check: 95 from: 1 to: 4244
pF1KB6577 4244 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1121 1282 1121
Size: 161 4083
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 860 2069 2501 3248 3516 611
Size: 249 1209 432 747 268 1339
Fragments arranged by size:
1339 1209 747 432 268 249
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AlwNI CAG_nnn'CTG
Cuts at: 373 1549 3116 373
Size: 1176 1567 1501
Fragments arranged by size:
1567 1501 1176
ApaLI G'TGCA_C
Cuts at: 3014 3014
Size: 4244
ApoI r'AATT_y
Cuts at: 136 234 448 1141 1248 3597 3853 136
Size: 98 214 693 107 2349 256 527
Fragments arranged by size:
2349 693 527 256 214 107 98
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 1264 1264
Size: 4244
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 1182 1260 1674 1922 1957 1182
Size: 78 414 248 35 3469
Fragments arranged by size:
3469 414 248 78 35
BanII G_rGCy'C
Cuts at: 1258 2288 1258
Size: 1030 3214
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BciVI GTATCCnnnnn_n'
Cuts at: 318 2142 2903 4244 318
Size: 1824 761 1341 318
Fragments arranged by size:
1824 1341 761 318
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 1356 1356
Size: 4244
Bme1580I G_kGCm'C
Cuts at: 1869 1962 3018 1869
Size: 93 1056 3095
Fragments arranged by size:
3095 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 680 1456 1537 1872 4016 680
Size: 776 81 335 2144 908
Fragments arranged by size:
2144 908 776 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 2299 2791 3089 1420
Size: 879 492 298 2575
Fragments arranged by size:
2575 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3948 3948
Size: 4244
BsaAI yAC'GTr
Cuts at: 1597 1597
Size: 4244
BsaBI GATnn'nnATC
Cuts at: 6 1758 3795 6
Size: 1752 2037 455
Fragments arranged by size:
2037 1752 455
BsaHI Gr'CG_yC
Cuts at: 1675 1923 2625 4121 1675
Size: 248 702 1496 1798
Fragments arranged by size:
1798 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1433 1954 2632 2906 3053 3464 1433
Size: 521 678 274 147 411 2213
Fragments arranged by size:
2213 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1669 2427 3004 3500 1669
Size: 758 577 496 2413
Fragments arranged by size:
2413 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1786 1832 3040 104
Size: 1682 46 1208 1308
Fragments arranged by size:
1682 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 294 1258 2036 2226 3018 294
Size: 964 778 190 792 1520
Fragments arranged by size:
1520 964 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 421 421
Size: 4244
Bsp1286I G_dGCh'C
Cuts at: 294 1258 1869 1962 2036 2226 2288 3018 294
Size: 964 611 93 74 190 62 730 1520
Fragments arranged by size:
1520 964 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3464 3464
Size: 4244
BspHI T'CATG_A
Cuts at: 3420 3420
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1294 1508 1810 2191 1294
Size: 214 302 381 3347
Fragments arranged by size:
3347 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2156 2156
Size: 4244
BsrFI r'CCGG_y
Cuts at: 2242 2423 2632 2242
Size: 181 209 3854
Fragments arranged by size:
3854 209 181
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 382 681 1621 382
Size: 299 940 3005
Fragments arranged by size:
3005 940 299
BstBI TT'CG_AA
Cuts at: 231 2605 231
Size: 2374 1870
BstEII G'GTnAC_C
Cuts at: 613 613
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 794 794
Size: 4244
BstYI r'GATC_y
Cuts at: 1 1269 1736 1759 2094 2340 3352 3438 3450
Size: 1268 467 23 335 246 1012 86 12
Cuts at: 3450 3796 1
Size: 346 449
Fragments arranged by size:
1268 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 330 330
Size: 4244
BtgI C'CryG_G
Cuts at: 107 1151 107
Size: 1044 3200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 531 1487 531
Size: 956 3288
DraI TTT'AAA
Cuts at: 1098 1243 1098
Size: 145 4099
DraIII CAC_nnn'GTG
Cuts at: 904 904
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 1829 2003 2394 2421 3766 1829
Size: 174 391 27 1345 2307
Fragments arranged by size:
2307 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2452 2762 2908 4054 2452
Size: 310 146 1146 2642
Fragments arranged by size:
2642 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 860 2069 2501 3248 3516 611
Size: 249 1209 432 747 268 1339
Fragments arranged by size:
1339 1209 747 432 268 249
EcoO109I rG'GnC_Cy
Cuts at: 1383 1383
Size: 4244
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 345 2263 345
Size: 1918 2326
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 1678 1926 2948 3927 1678
Size: 248 1022 979 1995
Fragments arranged by size:
1995 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 361 393 466 498 1024 1056 2086
Size: 32 130 32 73 32 526 32 1030
Cuts at: 2086 2116 2118 2148 199
Size: 30 2 30 2295
Fragments arranged by size:
2295 1030 526 130 73 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 402 1283 402
Size: 881 3363
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 149 543 1128 2915 3099 149
Size: 394 585 1787 184 1294
Fragments arranged by size:
1787 1294 585 394 184
MscI TGG'CCA
Cuts at: 2005 2005
Size: 4244
MslI CAynn'nnrTG
Cuts at: 2233 2360 2233
Size: 127 4117
MspA1I CmG'CkG
Cuts at: 1355 1669 2029 3042 3287 1355
Size: 314 360 1013 245 2312
Fragments arranged by size:
2312 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 4244
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NcoI C'CATG_G
Cuts at: 107 1151 107
Size: 1044 3200
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 4244
NspI r_CATG'y
Cuts at: 435 688 1297 1797 2704 435
Size: 253 609 500 907 1975
Fragments arranged by size:
1975 907 609 500 253
PasI CC'CwG_GG
Cuts at: 406 406
Size: 4244
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 4212
PstI C_TGCA'G
Cuts at: 1291 1976 1291
Size: 685 3559
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 1669 2029 1669
Size: 360 3884
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 1287 1972 2965 3156 33
Size: 1254 685 993 191 1121
Fragments arranged by size:
1254 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 1399 2314 2806 3068 1399
Size: 915 492 262 2575
Fragments arranged by size:
2575 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
StuI AGG'CCT
Cuts at: 918 918
Size: 4244
StyI C'CwwG_G
Cuts at: 107 949 1151 1378 1732 107
Size: 842 202 227 354 2619
Fragments arranged by size:
2619 842 354 227 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 521 601 1827 1960 2419 2628 521
Size: 80 1226 133 459 209 2137
Fragments arranged by size:
2137 1226 459 209 133 80
TatI w'GTAC_w
Cuts at: 3759 3759
Size: 4244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 273 305 3761 3793 273
Size: 32 3456 32 724
Fragments arranged by size:
3456 724 32 32
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1739 1739
Size: 4244
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI