Restriction map for
pF1KB6570
(Circular) (Six-base) MAPSORT of: pF1KB6570.seq Check: 3073 from: 1 to: 4313
pF1KB6570 4313 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 913 1128 913
Size: 215 4098
AccI GT'mk_AC
Cuts at: 530 1351 530
Size: 821 3492
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 1153 2138 2570 3317 3585 1147
Size: 6 985 432 747 268 1875
Fragments arranged by size:
1875 985 747 432 268 6
AfeI AGC'GCT
Cuts at: 717 717
Size: 4313
AflIII A'CryG_T
Cuts at: 1118 1665 2769 3746 1118
Size: 547 1104 977 1685
Fragments arranged by size:
1685 1104 977 547
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AleI CACnn'nnGTG
Cuts at: 1003 1228 1003
Size: 225 4088
AlwNI CAG_nnn'CTG
Cuts at: 584 1618 3185 584
Size: 1034 1567 1712
Fragments arranged by size:
1712 1567 1034
ApaI G_GGCC'C
Cuts at: 1030 1030
Size: 4313
ApaLI G'TGCA_C
Cuts at: 436 970 3083 436
Size: 534 2113 1666
Fragments arranged by size:
2113 1666 534
ApoI r'AATT_y
Cuts at: 1317 3666 3922 1317
Size: 2349 256 1708
Fragments arranged by size:
2349 1708 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 670 1333 670
Size: 663 3650
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 804 1329 1743 1991 2026 804
Size: 525 414 248 35 3091
Fragments arranged by size:
3091 525 414 248 35
BanII G_rGCy'C
Cuts at: 574 1030 1327 2357 574
Size: 456 297 1030 2530
Fragments arranged by size:
2530 1030 456 297
BbeI G_GCGC'C
Cuts at: 1747 1995 1747
Size: 248 4065
BbvCI CC'TCA_GC
Cuts at: 424 486 424
Size: 62 4251
BciVI GTATCCnnnnn_n'
Cuts at: 2211 2972 4313 2211
Size: 761 1341 2211
Fragments arranged by size:
2211 1341 761
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 1163 1425 1163
Size: 262 4051
Bme1580I G_kGCm'C
Cuts at: 440 809 974 1030 1938 2031 3087 440
Size: 369 165 56 908 93 1056 1666
Fragments arranged by size:
1666 1056 908 369 165 93 56
BmrI ACTGGGnnnn_n'
Cuts at: 186 1525 1606 1941 4085 186
Size: 1339 81 335 2144 414
Fragments arranged by size:
2144 1339 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 248 2680 248
Size: 2432 1881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 402
Size: 4313
Bpu10I CC'TnA_GC
Cuts at: 223 424 486 573 223
Size: 201 62 87 3963
Fragments arranged by size:
3963 201 87 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1489 2368 2860 3158 1489
Size: 879 492 298 2644
Fragments arranged by size:
2644 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 760 4017 760
Size: 3257 1056
BsaAI yAC'GTr
Cuts at: 441 1666 441
Size: 1225 3088
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 1744 1992 2694 4190 1744
Size: 248 702 1496 1867
Fragments arranged by size:
1867 1496 702 248
BsaWI w'CCGG_w
Cuts at: 296 1502 2023 2701 2975 3122 3533 296
Size: 1206 521 678 274 147 411 1076
Fragments arranged by size:
1206 1076 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 207 334 474 207
Size: 127 140 4046
Fragments arranged by size:
4046 140 127
BseYI C'CCAG_C
Cuts at: 856 866 1738 2496 3073 3569 856
Size: 10 872 758 577 496 1600
Fragments arranged by size:
1600 872 758 577 496 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 4313
BsiEI CG_ry'CG
Cuts at: 104 1855 1901 3109 104
Size: 1751 46 1208 1308
Fragments arranged by size:
1751 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 440 701 974 1327 2105 2295 3087 440
Size: 261 273 353 778 190 792 1666
Fragments arranged by size:
1666 792 778 353 273 261 190
Bsp1286I G_dGCh'C
Cuts at: 440 574 701 809 974 1030 1327 1938 2031
Size: 134 127 108 165 56 297 611 93
Cuts at: 2031 2105 2295 2357 3087 440
Size: 74 190 62 730 1666
Fragments arranged by size:
1666 730 611 297 190 165 134 127
108 93 74 62 56
BspEI T'CCGG_A
Cuts at: 3533 3533
Size: 4313
BspHI T'CATG_A
Cuts at: 3489 3489
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 913 1128 1363 1577 1879 2260 913
Size: 215 235 214 302 381 2966
Fragments arranged by size:
2966 381 302 235 215 214
BsrBI CCG'CTC
Cuts at: 50 2605 2659 4143 4308 50
Size: 2555 54 1484 165 55
Fragments arranged by size:
2555 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2225 2225
Size: 4313
BsrFI r'CCGG_y
Cuts at: 150 1146 2311 2492 2701 150
Size: 996 1165 181 209 1762
Fragments arranged by size:
1762 1165 996 209 181
BsrGI T'GTAC_A
Cuts at: 142 142
Size: 4313
BssHII G'CGCG_C
Cuts at: 811 811
Size: 4313
BssSI C'ACGA_G
Cuts at: 2584 2942 2584
Size: 358 3955
BstAPI GCAn_nnn'nTGC
Cuts at: 584 1690 584
Size: 1106 3207
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstYI r'GATC_y
Cuts at: 1 362 1338 1805 1828 2163 2409 3421 3507
Size: 361 976 467 23 335 246 1012 86
Cuts at: 3507 3519 3865 1
Size: 12 346 449
Fragments arranged by size:
1012 976 467 449 361 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 337 464 743 800 990 1184 107
Size: 230 127 279 57 190 194 3236
Fragments arranged by size:
3236 279 230 194 190 127 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 144 203 636 2263 2444 2607 144
Size: 59 433 1627 181 163 1850
Fragments arranged by size:
1850 1627 433 181 163 59
BtsI GCAGTG_nn'
Cuts at: 1556 1556
Size: 4313
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DraIII CAC_nnn'GTG
Cuts at: 969 969
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 2019 2877 2019
Size: 858 3455
EaeI y'GGCC_r
Cuts at: 312 1181 1898 2072 2463 2490 3835 312
Size: 869 717 174 391 27 1345 790
Fragments arranged by size:
1345 869 790 717 391 174 27
EagI C'GGCC_G
Cuts at: 1898 1898
Size: 4313
EarI CTCTTCn'nnn_
Cuts at: 188 2336 2546 188
Size: 2148 210 1955
Fragments arranged by size:
2148 1955 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 835 2521 2831 2977 4123 835
Size: 1686 310 146 1146 1025
Fragments arranged by size:
1686 1146 1025 310 146
EcoICRI GAG'CTC
Cuts at: 1325 1325
Size: 4313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 1147 1153 2138 2570 3317 3585 402
Size: 745 6 985 432 747 268 1130
Fragments arranged by size:
1130 985 747 745 432 268 6
EcoO109I rG'GnC_Cy
Cuts at: 405 1027 1240 1279 1452 405
Size: 622 213 39 173 3266
Fragments arranged by size:
3266 622 213 173 39
EcoRI G'AATT_C
Cuts at: 1317 1317
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FseI GG_CCGG'CC
Cuts at: 1150 1150
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 151 584 719 1747 1995 3017 3996 151
Size: 433 135 1028 248 1022 979 468
Fragments arranged by size:
1028 1022 979 468 433 248 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2155 2185 2187 2217 2155
Size: 30 2 30 4251
Fragments arranged by size:
4251 30 30 2
HincII GTy'rAC
Cuts at: 156 1352 156
Size: 1196 3117
KasI G'GCGC_C
Cuts at: 1743 1991 1743
Size: 248 4065
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2984 3168 2984
Size: 184 4129
MscI TGG'CCA
Cuts at: 1183 2074 1183
Size: 891 3422
MslI CAynn'nnrTG
Cuts at: 706 1003 1228 2302 2429 706
Size: 297 225 1074 127 2590
Fragments arranged by size:
2590 1074 297 225 127
MspA1I CmG'CkG
Cuts at: 126 339 368 411 428 490 833 870 1162
Size: 213 29 43 17 62 343 37 292
Cuts at: 1162 1303 1424 1738 2098 3111 3356 126
Size: 141 121 314 360 1013 245 1083
Fragments arranged by size:
1083 1013 360 343 314 292 245 213
141 121 62 43 37 29 17
NaeI GCC'GGC
Cuts at: 1148 2494 1148
Size: 1346 2967
NarI GG'CG_CC
Cuts at: 1744 1992 1744
Size: 248 4065
NcoI C'CATG_G
Cuts at: 107 1184 107
Size: 1077 3236
NgoMIV G'CCGG_C
Cuts at: 1146 2492 1146
Size: 1346 2967
NspI r_CATG'y
Cuts at: 753 1366 1866 2773 753
Size: 613 500 907 2293
Fragments arranged by size:
2293 907 613 500
PciI A'CATG_T
Cuts at: 2769 2769
Size: 4313
PflMI CCAn_nnn'nTGG
Cuts at: 950 1275 950
Size: 325 3988
PfoI T'CCnGG_A
Cuts at: 839 1250 839
Size: 411 3902
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PmlI CAC'GTG
Cuts at: 441 441
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 405 1279 405
Size: 874 3439
PspOMI G'GGCC_C
Cuts at: 1026 1026
Size: 4313
PstI C_TGCA'G
Cuts at: 347 1139 1360 2045 347
Size: 792 221 685 2615
Fragments arranged by size:
2615 792 685 221
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 428 870 1162 1303 1738 2098 428
Size: 442 292 141 435 360 2643
Fragments arranged by size:
2643 442 435 360 292 141
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 1327 1327
Size: 4313
SacII CC_GC'GG
Cuts at: 340 340
Size: 4313
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SanDI GG'GwC_CC
Cuts at: 405 405
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 188 2336 2546 188
Size: 2148 210 1955
Fragments arranged by size:
2148 1955 210
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 175 3830 175
Size: 3655 658
SexAI A'CCwGG_T
Cuts at: 965 965
Size: 4313
SfcI C'TryA_G
Cuts at: 33 343 1135 1356 2041 3034 3225 33
Size: 310 792 221 685 993 191 1121
Fragments arranged by size:
1121 993 792 685 310 221 191
SfoI GGC'GCC
Cuts at: 1745 1993 1745
Size: 248 4065
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 1468 2383 2875 3137 1468
Size: 915 492 262 2644
Fragments arranged by size:
2644 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 753 1366 1866 753
Size: 613 500 3200
Fragments arranged by size:
3200 613 500
StuI AGG'CCT
Cuts at: 349 349
Size: 4313
StyI C'CwwG_G
Cuts at: 107 1001 1184 1447 1801 107
Size: 894 183 263 354 2619
Fragments arranged by size:
2619 894 354 263 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1896 2029 2488 2697 1896
Size: 133 459 209 3512
Fragments arranged by size:
3512 459 209 133
TatI w'GTAC_w
Cuts at: 142 173 3828 142
Size: 31 3655 627
Fragments arranged by size:
3655 627 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4281
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1808 1808
Size: 4313
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
XmnI GAAnn'nnTTC
Cuts at: 457 457
Size: 4313
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PspXI
PsrI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI